A new Cosmocercoides species (Ascaridida: Cosmocercidae), C. wuyiensis n. sp., from the Asiatic frog Amolops wuyiensis (Amphibia: Anura)

2019 ◽  
Vol 94 ◽  
Author(s):  
Y. Liu ◽  
Q. Yu ◽  
Y.-L. Shu ◽  
J.-H. Zhao ◽  
J.-Y. Fang ◽  
...  
Keyword(s):  
18S Rdna ◽  
Phylogenetic Analyses ◽  
Nematode Species ◽  
Coi Gene ◽  
Male Body ◽  
Lizard Species ◽  
Nucleotide Divergence ◽  
Distinguishing Features ◽  
Morphological And Molecular Characterization

Abstract We identified and characterized a new cosmocercid nematode species, Cosmocercoides wuyiensis n. sp., through microscopic examination and sequencing of the partial small ribosomal RNA gene (18S rDNA), internal transcribed spacer (ITS) and mitochondrial cytochrome c oxidase subunit 1 (COI) genes. The new species was isolated from the intestine of the Asiatic frog Amolops wuyiensis Liu and Hu, 1975 captured from four localities of the Anhui province in south-east China. Among the 25 recorded species of the Cosmocercoides genus, the morphology of C. wuyiensis n. sp. is closest to that of C. kiliwai and C. malayensis, which were isolated from various Mexican frog and Malaysian lizard species, respectively. However, C. wuyiensis n. sp. displayed several distinguishing features, such as small size of the male body, two spicules of unequal lengths in the male, small gubernaculum, pre-, ad- and post-cloacal caudal rosette papillae in the ratio of 18–24:2:6 and simple papillae in the ratio of 14:multiple:4, circle and number of punctation in each rosette at 1:11–16, sharply conical tail-end and the presence of lateral alae and somatic papillae in both sexes. BLAST and the phylogenetic analyses of the 18S rDNA and ITS sequences indicated that C. wuyiensis n. sp. belonged to the genus Cosmocercoides, while that of the COI gene sequence of C. wuyiensis n. sp. showed 16.36% nucleotide divergence with C. pulcher and 47.99% nucleotide divergence with C. qingtianensis. The morphological and molecular characterization of C. wuyiensis n. sp. provides new taxonomic data for this genus.

Morphological and molecular characterization of Ortleppascaris sinensis sp. nov. (Nematoda: Ascaridoidea) from the Chinese alligator Alligator sinensis

2015 ◽  
Vol 90 (3) ◽  
pp. 303-311 ◽  
Author(s):  
J.H. Zhao ◽  
S.S. Wang ◽  
G.J. Tu ◽  
Y.K. Zhou ◽  
X.B. Wu
Keyword(s):  
Electron Microscopic Examination ◽  
Nematode Species ◽  
Small Subunit ◽  
Internal Transcribed Spacers ◽  
Coi Gene ◽  
Electron Microscopic ◽  
Chinese Alligator ◽  
Alligator Sinensis ◽  
Morphological And Molecular Characterization

AbstractA new nematode species, Ortleppascaris sinensis sp. nov. (Ascaridoidea), is described from specimens found in the stomach and intestine of the Chinese alligator Alligator sinensis Fauvel, 1879 (Crocodilian: Alligatoridae) in the National Nature Reserve of Chinese Alligator (Chinese Crocodile Lake) in Anhui Province, China. This is the first description of O. sinensis sp. nov. in both China and this crocodile host, increasing its distribution in South Asia as well as expanding the number of helminths known to infect this crocodile. The detailed description of O. sinensis sp. nov., based on light and scanning electron microscopic examination, provides new taxonomic data for this species, and we also report sequences of the internal transcribed spacers (ITS), small subunit DNA segments (18S) and the cytochrome oxidase I (COI) gene.

Morphological and molecular characterization of Trophurus wuhuensis n. sp. (Nematoda: Telotylenchinae) from soil associated with Cinnamomum camphora in China

2017 ◽  
Vol 92 (5) ◽  
pp. 637-644
Author(s):  
Y. Li ◽  
J. Du ◽  
Z.Y. Wang ◽  
K. Wang ◽  
H.L. Li
Keyword(s):  
Ribosomal Dna ◽  
18S Rdna ◽  
Nematode Species ◽  
Cinnamomum Camphora ◽  
Lateral Field ◽  
Plant Nematode ◽  
Internal Transcribed Spacer Sequences ◽  
Morphological And Molecular Characterization ◽  
Partial 18S

AbstractA new plant nematode species, Trophurus wuhuensis n. sp., was collected from the soil associated with Cinnamomum camphora in Wuhu, Anhui Province, China. The new species is characterized by having a female with a slender body 660.5–801.5 μm in length, stylet 12–14 μm long, knobs directed laterad, lateral field marked by short and scattered grooves, post-vulval uterine sac shorter than vulval body diameter, post-rectal intestinal sac absent, tail cylindroid, terminus with deep wrinkles; and male with a pointed tail terminus and spicules 16–18 μm long. The internal transcribed spacer sequences of ribosomal DNA (ITS rDNA) and partial 18S ribosomal DNA (18S rDNA) from T. wuhuensis n. sp. were amplified and sequenced. A phylogenetic analysis based on sequences of 18S rDNA fragments is given in this study.

Morphological and molecular characterization of Paractinolaimus sahandi n. sp. (Nematoda: Actinolaimidae) from the Sahand Mountains in Iran

2010 ◽  
Vol 85 (3) ◽  
pp. 276-282 ◽  
Author(s):  
M. Pedram ◽  
G. Niknam ◽  
M.T. Vinciguerra ◽  
W. Ye ◽  
R.T. Robbins
Keyword(s):  
New Species ◽  
Molecular Characterization ◽  
Outer Layer ◽  
18S Rdna ◽  
Soil Samples ◽  
28S Rdna ◽  
First Report ◽  
Morphological And Molecular Characterization

AbstractParactinolaimus sahandi n. sp., found in wet soil samples collected from the rhizosphere of grasses of Sahand Mountains, Iran, is described. This new species is characterized by its long body (3.5–4.7 mm), high a value (74.5–88.5), anterior location of posterior subventral nuclei, occupying 62.5–68.0% of glandularium distance, the presence of 1–4 pre- and 1–3 post-vulval papillae and numerous tiny, not innervated papillae in front and behind the vulva in the outer layer of cuticle; common functional males in the population, with 62.5–81.3 μm long spicules and 15–17 ventromedian supplements. The new species, which is the only one in the genus showing the advulval cuticular tiny papillae and is unusually slender, is compared to four species of Paractinolaimus, namely P. macrolaimus, P. longidrilus, P. spanithelus and P. rafiqi. The ribosomal 18S rDNA (1246 bp sequenced) and 28S rDNA D2/D3 region (844 bp sequenced) of P. sahandi n. sp. were sequenced for molecular characterization. Sequences of the 18S and 28S D2/D3 of P. sahandi n. sp. have distinct differences from those of the only sequenced P. macrolaimus, with 6 bp differences in 18S and 38 bp differences and five gaps in 28S. This is the first report of the occurrence of members of Actinolaimidae in Iran.

scholarly journals Morphological and molecular characterization of Haplorchoides mehrai Pande and Shukla 1976 (Digenea: Heterophyidae) from Chiang Mai province

2018 ◽  
Vol 55 (4) ◽  
pp. 334-342
Author(s):  
K. Apiwong ◽  
Ch. Wongsawad ◽  
P. Butboonchoo
Keyword(s):  
Phylogenetic Analysis ◽  
New Record ◽  
Adult Stage ◽  
Morphological Characteristics ◽  
Molecular Characteristic ◽  
Coi Gene ◽  
Chiang Mai ◽  
Chiang Mai Province ◽  
Morphological And Molecular Characterization

Abstract Cyprinoid fish in Chiang Mai province has been reported the presence of a large number of metacercariae, particularly the metacercariae of Haplorchoides and those not identified to species. This study aims to investigate morphological and molecular characteristic of the minute intestinal fluke H. mehrai metacercariae in two cyprinoid fish species from Chom Thong district, Chiang Mai province, Thailand: the Tinfoil barb (Barbonymus schwanenfeldii) and the White eye barb (Cyclocheilichthys repasson). A total of 180 fish (90 from B. schwanenfeldii and 90 from C. repasson) were collected over three seasons: cool, hot and the rainy season (December 2015 to August 2016). Fish were examined for H. mehrai metacercariae infection, including areas such as muscle and the inner side of body scales, by using a light microscope. The prevalence of H. mehrai metacercariae in B. schwanenfeldii and C. repasson was 73.33 % and 100 % respectively. Haplorchoides metacercariae were identified as H. mehrai based on the morphological characteristics; the position of the acetabulum and the number and arrangement of the acetabular spines. Phylogenetic analysis based on Cytochrome c Oxidase subunit I (COI) gene showed that H. mehrai metacercariae from B. schwanenfeldii and C. repasson were the same species as the adult stage of H. mehrai from Hemibagrus nemurus and Mystus multiradiatus. Both morphological and molecular characteristic could indicate that Haplorchoides metacercariae originated from this study were H. mehrai. Furthermore, it is a new record of the minute intestinal fluke Haplorchoides mehrai in Chiang Mai Province

scholarly journals Phenotypic and genetic characterization of date palm cultivars resistant to bayoud disease

2021 ◽  
Vol 8 (4) ◽  
Author(s):  
Said Boudeffeur ◽  
Abdelkhader Ameur Ameur ◽  
M M Aci ◽  
Hafida Khelafi ◽  
Abdelkader Laaboudi ◽  
...  
Keyword(s):  
Date Palm ◽  
Phylogenetic Analyses ◽  
Principal Component ◽  
Natural Resistance ◽  
Sequence Comparisons ◽  
Bayoud Disease ◽  
Phenotypic And Genetic Characterization ◽  
Simple Sequence ◽  
Morphological And Molecular Characterization

Taqerbucht cultivars of date palm are well known by their natural resistance against devastating fungus Bayoud disease. In order to know, if these accessions have the same genetic and morphological profile or each of them constitutes a separate cultivar, we carried out a morphological and molecular characterization and we compared four Taqerbucht (Tq.) date palm cultivars from the southwestern region of Algeria: Tq.hamra cultivar (red fruits), Tq. safra cultivar (yellow fruits), Tq.beïda (white fruits) and Tq.kahla cultivar (black fruits). Seventy one phenotypic characteristics, including 33 quantitative and 38 qualitative traits, have been selected for comparison. Principal component analysis (PCA) and multi-component clustering were used to analyze and compare the data. The results suggest that the four cultivars can be classified into distinct groups. One group contains one cultivar, the Tq.kahla and another group contains the three other cultivars (Tq.safra, Tq.beïda and Tq. hamra). Based on phylogenetic analyses and sequence comparisons, the cultivar Tq. kahla seems to be divergent from the cultivar Tq.hamra, whereas the two cultivars Tq.Safra and Tq.beïda are close to each other. Using 16 Simple Sequence Repeat (SSR) genetic markers to analyze genetic diversity among the cultivars, we found that 13 markers were detectable in 31 allele's loci, and the number of alleles per locus varied from 1–4 with an average of 2.38 alleles per locus. Expected heterozygosity (He) values ranged from 0.375–0.500 and observed heterozygosity (Ho) values from 0.750–1.000.

Morphological and Molecular Characterization of Heterobothrium indicus n. sp. (Monogenea: Diclidophoridae) and Its Phylogenetic Status

2021 ◽  
Author(s):  
A.K. Verma
Keyword(s):  
New Species ◽  
18S Rdna ◽  
Arabian Sea ◽  
28S Rdna ◽  
Coi Gene ◽  
Western Coast ◽  
Monogenean Parasite ◽  
Morphological Studies ◽  
Phylogenetic Status

Background: A new species Heterobothrium indicus n. sp. was isolated from the gills of Upeneus moluccensis (Bleeker, 1855) from the Western Coast of India in Arabian Sea region. The monogenean parasite differs from other congeners by morphological features like the presence of asymmetrical haptoral region, 4th pair of clamp smallest than other three pairs of clamps, genital atrium with 8 hooks, number of testes 29-37 and absence of isthmus.Methods: During the survey of marine fishes at Arabian Sea region, new species of monogenean parasite was isolated from the gills of marine fish Upeneus moluccensis. The parasites were morphologically characterized with the help of light and phase contrast microscopy. 18S rDNA, 28S rDNA, mt COI, ITS1+5.8S and ITS2+5.8S gene regions of parasites were amplified, sequenced and compared with other diclidophorid taxa using different bioinformatics tools.Result: Phylogenetic tree analyses (NJ, ME and MP methods) of 18S rDNA, 28S rDNA and mt COI gene regions are complementing the morphological studies and clearly suggested the placement of this new species under subfamily Choricotylinae, family Diclidophoridae.

Morphological and molecular characterization of Hemicycliophora subbotini n. sp. (Tylenchida: Hemicycliophoridae) from China

Zootaxa ◽  
2018 ◽  
Vol 4433 (1) ◽  
pp. 161 ◽  
Author(s):  
MUNAWAR MARIA ◽  
RUIHANG CAI ◽  
NAN QU ◽  
PABLO CASTILLO ◽  
JINGWU ZHENG
Keyword(s):  
New Species ◽  
Phylogenetic Analyses ◽  
Oral Disc ◽  
Pharyngeal Bulb ◽  
En Face ◽  
The Status ◽  
A New Species ◽  
Morphological And Molecular Characterization ◽  
Lateral Plates

Sheath nematodes of the genus Hemicycliophora are migratory root-ectoparasites of many crops, but the damage is documented for only a few species. Hemicycliophora subbotini n. sp., isolated in Hangzhou, Zhejiang, China, from the rhizosphere of camphor tree (Cinnamomum camphora (L.) Presl), is described and characterized morphologically and molecularly with important morphological details elucidated by SEM studies. The new species can be characterized by the conoid lip region, with bulging oral disc protruding beyond the first annulus in some specimens. Oral disc narrow ovate, amphidial apertures covered by lateral plates, lying in the same plane as oral disc when observed en face. Stylet 118 (108–125) μm long and flexible, stylet knobs concave shaped having a slight cavity. The excretory pore is located 1–2 annuli posterior to the base of pharyngeal bulb. Vulval lips slightly modified with small vulval sleeve, spermatheca rounded to oblong. Anus located 18 (15–22) annuli posterior to vulva. Tail elongated, with conoid or slightly offset spike having a narrowly rounded terminus. The study provides the first description of a new Hemicycliophora species from China. Phylogenetic analyses based on D2–D3 expansion segments of the 28S and ITS fragments revealed the new species forming a separate clade from other Hemicycliophora species which supported the status as a new species of this genus. 

Meloidogyne lopezi n. sp. (Nematoda: Meloidogynidae), a new root-knot nematode associated with coffee (Coffea arabica L.) in Costa Rica, its diagnosis and phylogenetic relationship with other coffee-parasitising Meloidogyne species

Nematology ◽  
2014 ◽  
Vol 16 (6) ◽  
pp. 643-661 ◽  
Author(s):  
Danny A. Humphreys-Pereira ◽  
Danny A. Humphreys-Pereira ◽  
Lorena Flores-Chaves ◽  
Danny A. Humphreys-Pereira ◽  
Lorena Flores-Chaves ◽  
...  
Keyword(s):  
Costa Rica ◽  
16S Rrna ◽  
Coffea Arabica ◽  
Phylogenetic Analyses ◽  
Mitochondrial Gene ◽  
Restriction Enzymes ◽  
Nematode Species ◽  
Male Body ◽  
Root Knot Nematode ◽  
Pcr Rflp

Coffee (Coffea arabica L. cv. Catuai) seedlings with abundant small root galls caused by an unknown root-knot nematode were found in southern Costa Rica. Morphology, esterase and malate dehydrogenase isozyme phenotypes and DNA markers differentiated this nematode from known Meloidogyne spp. A new species, M. lopezi n. sp., with common name Costa Rican root-knot nematode, is suggested. Meloidogyne lopezi n. sp. is distinguished from other coffee-associated Meloidogyne spp. by size of female lips and stylet, male body length and stylet and second-stage juvenile body and tail morphology. The region of the mitochondrial genome between COII and 16S rRNA showed a unique amplicon size of 1370 bp, and digestions with restriction enzymes HinfI, AluI, DraI and DraIII revealed characteristic PCR-RFLP patterns that differed from the tropical root-knot nematode species M. arabicida, M. incognita, M. izalcoensis, M. javanica and M. paranaensis. Characterisation of the protein-coding map-1 gene and phylogenetic analyses suggested that M. lopezi n. sp. might reproduce by mitotic parthenogenesis. Phylogenies estimated using Bayesian analyses based on the region between the COII and 16S rRNA mitochondrial genes, as well as the 18S and 28S ribosomal nuclear genes, indicated that M. lopezi n. sp. is closely related to other tropical Meloidogyne spp. that infect coffee, especially M. arabicida, M. izalcoensis and M. paranaensis from Central and South America. Isozyme analyses and PCR-RFLP of the COII-16S rRNA mitochondrial gene region enable a clear diagnostic differentiation between these species.

scholarly journals Morphological and Molecular Characterization of Nothotylenchus medians and N. similis (Nematoda: Anguinidae) from Southern Alberta, Canada

Horticulturae ◽  
2022 ◽  
Vol 8 (1) ◽  
pp. 74
Author(s):  
Maria Munawar ◽  
Atta Ur Rahman ◽  
Pablo Castillo ◽  
Dmytro P. Yevtushenko
Keyword(s):  
Molecular Characterization ◽  
Phylogenetic Analyses ◽  
Original Description ◽  
Distribution Data ◽  
Plant Parts ◽  
Full Diversity ◽  
Canadian Population ◽  
Inventory Survey ◽  
Morphological And Molecular Characterization

The nematode family, Anguinidae, is a diverse group of polyphagous nematodes, generally known as fungal feeders or parasites of aerial plant parts. Here, we present the morphological and molecular characterization of adult females of two Nothotylenchus species, N. medians and N. similis, along with host association and geographical distribution data of the genus. Both species are recorded as new reports from Canada and designated as reference populations for future studies. Morphological or morphometrical variation was not observed in the Canadian population of N. medians and N. similis, in comparison with the original description. Phylogenetic analyses based on 18S and D2–D3 of 28S genes placed both species within Anguinidae. Since the biology of the genus Nothotylenchus has not been rigorously characterized, the habitat and distribution information presented in this study will shed some light on the ecology of these nematodes. Notably, the detection of N. medians and N. similis in our nematode inventory survey indicates that considerable Nothotylenchus diversity is hidden in these soils. Consequently, increased surveys and more in-depth research are needed to explore the full diversity of anguinids inhabiting these cultivated areas.

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