Steinernema khuongin. sp. (Panagrolaimomorpha, Steinernematidae), a new entomopathogenic nematode species from Florida, USA

2018 ◽  
Vol 93 (2) ◽  
pp. 226-241 ◽  
Author(s):  
S.P. Stock ◽  
R. Campos-Herrera ◽  
F.E. El-Borai ◽  
L.W. Duncan
Keyword(s):  
New Species ◽  
Sequence Data ◽  
Novel Species ◽  
Entomopathogenic Nematode ◽  
Biological Species ◽  
Morphological Data ◽  
Biological Species Concept ◽  
The Novel ◽  
Ecological Data ◽  
Cross Hybridization

AbstractIn this study, molecular (ribosomal sequence data), morphological and cross-hybridization properties were used to identify a newSteinernemasp. from Florida, USA. Molecular and morphological data provided evidence for placing the novel species into Clade V, or the ‘glaseri-group’ ofSteinernemaspp. Within this clade, analysis of sequence data of the rDNA genes, 28S and internal transcribed spacer (ITS), depicted the novel species as a distinctive entity and closely related toS. glaseriandS. cubanum.Additionally, cross-hybridization assays showed that the new species is unable to interbreed with either of the latter two species, reinforcing its uniqueness from a biological species concept standpoint. Key morphological diagnostic characters forS. khuongin. sp. include the mean morphometric features of the third-stage infective juveniles: total body length (average: 1066 μm), tail length (average: 65 μm), location of the excretory pore (average: 80.5 μm) and the values ofc(average: 16.4),D% (average: 60.5),E% (average: 126) andH% (average: 46.6). Additionally, males can be differentiated fromS. glaseriandS. cubanumby the values of several ratios:D% (average: 68),E% (average: 323) and SW% (average: 120). The natural distribution of this species in Florida encompasses both natural areas and citrus groves, primarily in shallow groundwater ecoregions designated as ‘flatwoods’. The morphological, molecular, phylogenetic and ecological data associated with this nematode support its identity as a new species in theS. glaseri-group.

Steinernema innovationi n. sp. (Panagrolaimomorpha: Steinernematidae), a new entomopathogenic nematode species from South Africa

2014 ◽  
Vol 89 (4) ◽  
pp. 415-427 ◽  
Author(s):  
H. Çimen ◽  
M.-M. Lee ◽  
J. Hatting ◽  
S. Hazir ◽  
S.P. Stock
Keyword(s):  
South Africa ◽  
Sequence Data ◽  
Entomopathogenic Nematode ◽  
Mitochondrial Gene ◽  
Tail Length ◽  
Nematode Species ◽  
Total Body Length ◽  
Morphological Data ◽  
Cross Hybridization ◽  
Molecular Sequence Data

AbstractMorphological and molecular sequence data were combined with cross-hybridization studies and used to identify a new Steinernema sp. from Free State, South Africa. Molecular and morphological data indicate that the new species belongs to the ‘glaseri-group’ of Steinernema spp. Key morphological diagnostic characters for S. innovationi n. sp. include the morphometric features of the third-stage infective juveniles: total body length = 1054 (1000–1103) μm, tail length = 108 (97–117) μm, location of the excretory pore = 88 (82–91) μm, and D% = 58 (54–63), E% = 115 (104–137) and H% = 43 (37–46). Additionally, the morphology of the spicules and gubernaculum of the first-generation males are considered key diagnostic traits. Steinernema innovationi n. sp. was also characterized by analysis of both rDNA and mitochondrial gene sequence data, which further indicate the uniqueness of this Steinernema species.

scholarly journals A new planthopper species in the genus Omolicna (Hemiptera: Auchenorrhyncha: Derbidae) from the Reserva Privada el Silencio de Los Angeles Cloud Forest in Costa Rica

Zootaxa ◽  
2021 ◽  
Vol 4975 (2) ◽  
pp. 357-368
Author(s):  
MARCO A. ZUMBADO ECHAVARRIA ◽  
EDWIN A. BARRANTES BARRANTES ◽  
CHARLES R. BARTLETT ◽  
ERICKA E. HELMICK ◽  
BRIAN W. BAHDER
Keyword(s):  
New Species ◽  
Costa Rica ◽  
Los Angeles ◽  
Cytochrome C Oxidase ◽  
Sequence Data ◽  
Novel Species ◽  
Cloud Forest ◽  
Light Trapping ◽  
Phylogenetic Analyses ◽  
The Novel

Recent survey work on palms in Costa Rica has resulted in the discovery of several new species of Derbidae, especially in the Cenchreini. During a recent expedition, specimens collected by light trapping at the Hotel Villa Blanca (cloud forest) were determined to be a novel species of Omolicna Fennah. Sequence data was generated for the novel taxon for the cytochrome c oxidase subunit I (COI) and 18S loci. Pairwise analyses and phylogenetic analyses support placement of the novel taxon in Omolicna. 

Heterorhabditis gerrardi n. sp. (Nematoda: Heterorhabditidae): the hidden host of Photorhabdus asymbiotica (Enterobacteriaceae: γ-Proteobacteria)

2009 ◽  
Vol 83 (4) ◽  
pp. 309-320 ◽  
Author(s):  
K.L. Plichta ◽  
S.A. Joyce ◽  
D. Clarke ◽  
N. Waterfield ◽  
S.P. Stock
Keyword(s):  
New Species ◽  
Sequence Data ◽  
Entomopathogenic Nematode ◽  
Tail Length ◽  
Its Region ◽  
Nematode Species ◽  
Diagnostic Features ◽  
Cross Hybridization ◽  
Group V ◽  
Terminal Set

AbstractA new entomopathogenic nematode species from Australia, Heterorhabditis gerrardi n. sp. (Nematoda: Heterorhabditidae) is described. Morphological and molecular studies together with cross-hybridization tests indicated that this nematode represents a new undescribed species, closely related to members in the ‘indica-group’. However, the new species can be distinguished from other species in this genus by a combination of several qualitative and quantitative morphological traits. Key diagnostic features include: body size and excretory pore position of the third-stage infective juveniles; male bursa with a reduction of bursal rays, usually affecting the terminal set of papillae, with symmetrical or asymmetrical loss of one or two pairs; vulva of hermaphrodites more anteriorly located than in other species in the indica-group (V% average: 43), with non-protruding or slightly protruding lips, and longer tail length (average: 106 μm). The new species can be further characterized by molecular traits of sequence data from the internal transcribed spacer (ITS) region of ribosomal DNA. Additionally, the bacterial symbiont of this new species, Photorhabdus asymbiotica Kingscliff strain, was phenotypically characterized and compared with other P. asymbiotica strains. The Kingscliff strain revealed many characters not present in other strains of this species. We hypothesize that the newly found traits may contribute to the maintenance of this mutualistic association of the bacterium with its nematode host.

­­­Laccaria rubroalba sp. nov. (Hydnangiaceae, Agaricales) from Southwestern China

Phytotaxa ◽  
2016 ◽  
Vol 284 (1) ◽  
pp. 41 ◽  
Author(s):  
XIA LUO ◽  
LEI YE ◽  
JIE CHEN ◽  
SAMANTHA C. KARUNARATHNA ◽  
JIANCHU XU ◽  
...  
Keyword(s):  
New Species ◽  
Sequence Data ◽  
Novel Species ◽  
Morphological Characteristics ◽  
Molecular Data ◽  
Southwestern China ◽  
Its Sequence ◽  
The Novel ◽  
Closely Related Species ◽  
Line Drawings

The novel species Laccaria rubroalba is described from Southwestern China by using both morphological characteristics and molecular data. It is characterized by small basidiomata; reddish white pileus when moist or young, white to pale when dry; 4-spored basidia and globose to broadly ellipsoid, hyaline, moderately echinulate basidiospores. Phylogenetic relationships inferred from ITS sequence data confirmed the separation of this new species from other closely related species in the genus Laccaria. The new species is compared with similar taxa. A description, line drawings and colour photographs of the new species, and phylogenetic tree to show the placement of the new species are provided.

scholarly journals Mismatch induced speciation in Salmonella : model and data

2006 ◽  
Vol 361 (1475) ◽  
pp. 2045-2053 ◽  
Author(s):  
Daniel Falush ◽  
Mia Torpdahl ◽  
Xavier Didelot ◽  
Donald F Conrad ◽  
Daniel J Wilson ◽  
...  
Keyword(s):  
New Species ◽  
Natural Selection ◽  
Dna Sequence ◽  
Computer Simulations ◽  
De Novo ◽  
Sequence Data ◽  
Biological Species ◽  
Species Boundaries ◽  
Population Subdivision ◽  
Geographical Population

In bacteria, DNA sequence mismatches act as a barrier to recombination between distantly related organisms and can potentially promote the cohesion of species. We have performed computer simulations which show that the homology dependence of recombination can cause de novo speciation in a neutrally evolving population once a critical population size has been exceeded. Our model can explain the patterns of divergence and genetic exchange observed in the genus Salmonella , without invoking either natural selection or geographical population subdivision. If this model was validated, based on extensive sequence data, it would imply that the named subspecies of Salmonella enterica correspond to good biological species, making species boundaries objective. However, multilocus sequence typing data, analysed using several conventional tools, provide a misleading impression of relationships within S. enterica subspecies enterica and do not provide the resolution to establish whether new species are presently being formed.

scholarly journals Heterorhabditis noenieputensis n. sp. (Rhabditida: Heterorhabditidae), a new entomopathogenic nematode from South Africa

2012 ◽  
Vol 88 (2) ◽  
pp. 139-151 ◽  
Author(s):  
A.P. Malan ◽  
R. Knoetze ◽  
L. Tiedt
Keyword(s):  
South Africa ◽  
New Species ◽  
Internal Transcribed Spacer ◽  
Morphological Traits ◽  
Sequence Data ◽  
Entomopathogenic Nematode ◽  
The Body ◽  
Species Comparison ◽  
Citrus Orchard ◽  
A New Species

AbstractA new entomopathogenic nematode in the genus Heterorhabditis is described from South Africa, from two singular isolates found 1000 km from each other, from beneath a fig tree and in a citrus orchard, respectively. Morphological and molecular studies indicate both isolates to be the same and a new undescribed Heterorhabditis species. Comparison of sequences of the internal transcribed spacer (ITS) rDNA and the D2D3 region of the 28S rDNA gene with available sequences of other described species within the genus, indicate the two isolates as a new species. Phylogenetic analysis of the sequence data concerned placed the new species, H. noenieputensis n. sp., closest to H. indica and H. gerrardi in the indica-group. The new species, H. noenieputensis n. sp., is distinguished from other species in the genus by a combination of several morphological traits of the males and the infective juveniles (IJs). The new species differs from all other species previously described, as regards the body length of the IJs, except for H. indica and H. taysearae, in which the IJ is smaller. The IJ also differs from that of H. indica in the length of the oesophagus, the body diameter, the length of the tail and the E%. In addition, males of H. noenieputensis n. sp. differ from their closest relative, H. indica, in the position of the excretory pore, SW% and D%; and from H. gerrardi in the length of the oesophagus and SW%. The seventh pair of genital papillae of H. noenieputensis n. sp. are normally developed, while for H. indica they are often branched or swollen at the base, while 8 and 9 are usually absent in both species.

New subgenus and new species of marine benthic ostracods of genus Doloria (Ostracoda; Myodocopina) from the Southern Ocean

Zootaxa ◽  
2012 ◽  
Vol 3356 (1) ◽  
pp. 1 ◽  
Author(s):  
VLADIMIR G. CHAVTUR ◽  
SIMONE N. BRANDÃO ◽  
ALEXANDER G. BASHMANOV
Keyword(s):  
New Species ◽  
Southern Ocean ◽  
Deep Sea ◽  
Novel Species ◽  
The Other ◽  
The Novel ◽  
Atlantic Sector ◽  
New Subgenus ◽  
Fifth Limb ◽  
Water Depths

The project ANDEEP was designed to fill gaps in the knowledge of the biodiversity of the Southern Ocean deep sea. Threeoceanographic cruises (ANDEEP I, II and III) were undertaken in 2002 and 2005 in the Atlantic Sector of the SouthernOcean. Hundreds of samples were collected from 40 stations with water depths ranging from 748 to 6,348 m. Investiga-tions were carried out on a broad range of taxa, including bacteria, meio-, macroand megafauna. Approximately 5000ostracods were collected, which included 29 specimens of Doloria (Dolorietta) subgen. nov.The subdivision of the genus Doloria Skogsberg, 1920 into two subgenera, Doloria (Doloria) and the novel subgenusDoloria (Dolorietta), is based on differences in the structures of the fifth limb, the armature of the sensory bristle on the5th segment of the antennula, and the number of bristles on the 4th endite of the sixth limb. Four new species in the newsubgenus were identified from the ANDEEP samples. The new subgenus and the four novel species are described andillustrated. Two of these new species are named Doloria (Dolorietta) antarctica sp. nov. and Doloria (Dolorietta) sextafiliformis sp. nov., but the other two species are left in open nomenclature (i.e. Doloria (Dolorietta) sp. nov. 1 and Doloria(Dolorietta) sp. nov. 2) because only juveniles were found in our material. Keys to the subgenera and species of Doloria are provided.

scholarly journals A new species of the blind cave gudgeon Milyeringa (Pisces: Gobioidei, Eleotridae) from Barrow Island, Western Australia, with a redescription of M. veritas Whitley

Zootaxa ◽  
2013 ◽  
Vol 3616 (2) ◽  
pp. 135-150 ◽  
Author(s):  
HELEN K. LARSON ◽  
RALPH FOSTER ◽  
WILLIAM F. HUMPHREYS ◽  
MARK I. STEVENS
Keyword(s):  
New Species ◽  
Dna Sequence ◽  
Western Australia ◽  
Sequence Data ◽  
Morphological Data ◽  
Unique Form ◽  
Dna Sequence Data ◽  
The Third ◽  
Blind Cave ◽  
A New Species

A new species of the eyeless eleotrid genus Milyeringa is described from wells sunk on Barrow Island, Western Australia. Milyeringa justitia n. sp. is the third species of the genus to be named. Morphological data and cytochrome c oxidase subunit I (COI) DNA sequence data from a wide sample of localities at which the genus occurs was used to evaluate relationships and species limits. Milyeringa veritas is redescribed, and M. brooksi is synonymised with M. veritas. The unique form and ecology of these fishes, plus the threats to their survival, warrants immediate and continuing attention in management.

Tetraploa aquatica (Tetraplosphaeriaceae), a new freshwater fungal species from Yunnan Province, China

Phytotaxa ◽  
2020 ◽  
Vol 459 (2) ◽  
pp. 181-189
Author(s):  
WEN-LI LI ◽  
DAN-FENG BAO ◽  
D. JAYARAMA BHAT ◽  
HONG-YAN SU
Keyword(s):  
New Species ◽  
Yunnan Province ◽  
Sequence Data ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Fungal Species ◽  
Asexual Morph ◽  
Data Support ◽  
Decaying Wood

Asexual morph members of Tetraplosphaeriaceae (Pleosporales, Dothideomycetes) are generally hyphomycetes and usually found as saprobes occurring on various hosts in lentic habitats. Two isolates of a novel species, Tetraploa aquatica sp. nov., was collected from submerged decaying wood in Dulong river and a stream of Gaoligongshan mountain in Yunnan Province, China. The new species is characterized by short-cylindrical conidia, composed of 4-euseptate, short-cylindrical vertical columns which are verrucose at the base and with 4-setose vertical septate appendages. Tetraploa aquatica can be easily distinguished from other Tetraploa species by their columns which are 2–3-septate, aguttulate, vertical, setose appendaged which tend to remain parallel to one another apically. Phylogenetic analyses of combined LSU, ITS and SSU sequence data support its natural placement in Tetraploa. Detailed description and illustrations of this species and comparisons with other morphologically similar taxa in Tetraploa are provided.

Taxonomy, morphology and molecular systematics of three oligotrich ciliates, including a description of Apostrombidium parakielum spec. nov. (Ciliophora, Oligotrichia)

2013 ◽  
Vol 63 (Pt_3) ◽  
pp. 1179-1191 ◽  
Author(s):  
Wen Song ◽  
Jiamei Li ◽  
Weiwei Liu ◽  
Jiamei Jiang ◽  
Khaled A. S. Al- Rasheid ◽  
...  
Keyword(s):  
Sequence Data ◽  
Novel Species ◽  
Small Subunit ◽  
Rrna Gene ◽  
Ssu Rrna ◽  
The Novel ◽  
Small Subunit Rrna ◽  
Ssu Rrna Gene ◽  
Single Clade ◽  
Ssu Rrna Gene Sequence

Three oligotrich ciliates, Apostrombidium parakielum spec. nov., Novistrombidium apsheronicum (Alekperov & Asadullayeva, 1997) Agatha, 2003 and Novistrombidium testaceum (Anigstein, 1914) Song & Bradbury, 1998 were collected from the coastal waters of China and their morphology and small-subunit rRNA (SSU rRNA) gene sequences were studied. The novel species can be recognized by the combination of its obconical body shape, 14–16 anterior and 6–8 ventral membranelles, somatic kinety in three parts and conspicuously long dorsal cilia. Based on the data obtained for this novel species, an improved diagnosis of the genus Apostrombidium is supplied. Descriptions of the population of N. apsheronicum and N. testaceum collected in this study are also provided and compared with the existing descriptions. In addition, the phylogenetic positions of these three species are inferred from their SSU rRNA gene sequence data. The results indicate that the genus Apostrombidium, the systematics of which has not previously been discussed using molecular information, clusters with Varistrombidium kielum and Omegastrombidium elegans, whereas N. testaceum and N. apsheronicum form a single clade.

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