New and known species of the genus Pungentus Thorne & Swanger, 1936 (Dorylaimida, Nordiidae) from Iran

Abstract This contribution provides morphological and molecular data for one new and one known species of the genus Pungentus. The first species, P. azarbaijanensis n. sp., was recovered from the rhizospheric soil of grasses, collected in West Azarbaijan province, Iran, and was characterized by 2082–2365 μm long females having an angular lip region separated from the rest of the body by a constriction, 33–35 μm long odontostyle, vulva at 43.5–51.0%, 27.0–29.5 μm long rounded-conoid tail, and males unknown. It was compared morphologically with five species: P. angulosus, P. crassus, P. marietani, P. parapungens and P. pungens, which have didelphic-amphidelphic female reproductive system, body longer than 1.5 mm, and odontostyle longer than 20 μm. The second species, P. engadinensis, was recovered in three different regions of Iran (Mazandaran, Semnan and East Azarbaijan provinces). It was compared morphologically with some other populations reported all over the world. Besides morphological studies, molecular phylogenetic studies using partial sequences of 28S rDNA D2-D3 fragments were performed, and the phylogenetic relations of the two Iranian populations with other species and genera were discussed.

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The occurrence of three species of the genus Oscheius Andrássy, 1976 (Nematoda: Rhabditida) in Iran

Journal of Plant Protection Research ◽

10.1515/jppr-2017-0035 ◽

2017 ◽

Vol 57 (3) ◽

pp. 248-255 ◽

Cited By ~ 4

Author(s):

Ahmad Valizadeh ◽

Shila Goldasteh ◽

Zahra Rafiei-Karahroodi ◽

Majid Pedram

Keyword(s):

Morphological Characters ◽

Molecular Data ◽

Parasitic Nematodes ◽

Bayesian Posterior Probability ◽

Agricultural Pests ◽

Phylogenetic Studies ◽

Dead Trees ◽

Molecular Phylogenetic ◽

Morphological And Molecular Data ◽

First Time

AbstractDue to importance and effectiveness of some entomopathogenic or insect parasitic nematodes in controlling of agricultural pests, or pests of non-agricultural plants, a study was conducted in order to identify the species of this group of nematodes in city of Tehran. As the result, three species belonging to the genusOscheiuswere recovered in association with bark samples having the bark beetle galleries. Morphological and molecular data were provided for two recently recovered species of the genus, namelyO. necromenusandO. onirici. Molecular data were also provided for a recently recovered isolate ofO. tipulae. All three species were recovered in association with bark samples collected from dead trees in the city of Tehran. Morphological characters and morphometric data of the two aforementioned species are in accordance with the data given in their original descriptions. One recovered individual from a small bark sample characterized by its short body length was sequenced for its 28S and internal transcribed spacer (ITS) rDNA loci, and the results of BLAST search using the newly obtained partial sequences revealed that it belonged toO. tipulae. Molecular phylogenetic studies revealed recently sequenced Iranian populations ofO. oniriciandO. tipulaeforming a clade with other isolates/populations of these species in ITS tree with maximal Bayesian posterior probability (BPP), and presently sequenced isolates ofO. tipulaeandO. necromenusform a clade with other isolates of these species in 28S tree. The two speciesO. oniriciandO. necromenuswere reported in Iran for the first time.

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Heterorhabditis mexicana n. sp. (Rhabditida: Heterorhabditidae) from Tamaulipas, Mexico, and morphological studies of the bursa of Heterorhabditis spp.

Nematology ◽

10.1163/1568541041218031 ◽

2004 ◽

Vol 6 (2) ◽

pp. 231-244 ◽

Cited By ~ 27

Author(s):

Khuong Nguyen ◽

David Sharpiro-Ilan ◽

Robin Stuart ◽

Clay McCoy ◽

Rosalind James ◽

...

Keyword(s):

New Species ◽

Its Region ◽

Molecular Data ◽

The Body ◽

Sister Taxon ◽

Infective Juvenile ◽

Body Width ◽

Morphological Studies ◽

Morphological And Molecular Data ◽

A New Species

Abstract A new species of nematode in the genus Heterorhabditis was found in the northern part of the state of Tamaulipas, Mexico. Morphological and molecular data indicate that this nematode is a new species. The new species is described as Heterorhabditis mexicana n. sp. and is a sister taxon to H. indica. Heterorhabditis mexicana n. sp. is morphologically similar to H. bacteriophora, H. brevicaudis and H. indica and can be distinguished from these species mainly by male and female characters. Of the examined specimens of H. mexicana n. sp., 70% of males have eight pairs of bursal papillae, compared to nine in all other species. The ratio of the gubernaculum to spicule length (GS ratio) is higher than that of H. bacteriophora, H. brevicaudis and H. indica and the length of the spicule relative to anal body width (SW) is lower than all other species. For females, the vulval form of the new species is quite different from that of those species with a similar morphology (i. e ., H. bacteriophora and H. indica) and more closely resembles that of H. zealandica. The new species can also be distinguished from H. megidis, H. zealandica and H. marelatus by the body length, pharynx length of the infective juvenile and D% (distance from anterior end/pharynx length × 100), GS and SW ratios of males. In the ITS region of the rDNA tandem repeating unit, H. mexicana n. sp. has evolved 13 autapomorphic nucleotide character states, differing from its sister taxon H. indica at 113 aligned positions. The morphological and molecular data are sufficient to identify cladogenesis and delimit H. mexicana n. sp. as evolving independently from the other members of the genus.

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First record of the genus Parasitorhabditis Fuchs, 1937 (Rhabditida, Nematoda) from Iran with notes on morphological and molecular characters of the Iranian population of P. obtusa (Fuchs, 1915) Chitwood & Chitwood, 1950

Zootaxa ◽

10.11646/zootaxa.4353.3.13 ◽

2017 ◽

Vol 4353 (3) ◽

pp. 591

Author(s):

AHMAD VALIZADEH ◽

SHILA GOLDASTEH ◽

ZAHRA RAFIEI-KARAHROODI ◽

MAJID PEDRAM

Keyword(s):

Bark Beetles ◽

Nematode Species ◽

First Record ◽

Morphological Characters ◽

Molecular Data ◽

The Body ◽

Bayesian Posterior Probability ◽

Molecular Phylogenetic ◽

Morphological And Molecular Data ◽

Mediterranean Pine

Parasitorhabditis obtusa is reported from Iran for the first time. The studied population is characterised with its morphological and molecular data. Morphological characters of the recovered population is in agreement with the currently available data of the species. In molecular phylogenetic studies using partial sequences of 28S rDNA D2/D3 fragment, the species formed a clade with two other isolates of the species with maximal Bayesian posterior probability (1.00) and maximum likelihood bootstrap values (100). The species was in association with bark samples of dead coniferous trees having galleries of bark beetles. It was also recovered from inside of the body of the Mediterranean pine beetle, Orthotomicus erosus, representing a new insect host for this nematode species.

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The systematics of Boulengerula fischeri (Amphibia: Gymnophiona: Caeciliidae) based on morphological and molecular data

Zootaxa ◽

10.11646/zootaxa.2767.1.2 ◽

2011 ◽

Vol 2767 (1) ◽

pp. 14 ◽

Cited By ~ 5

Author(s):

DAVID J. GOWER ◽

ANNA PAPADOPOULOU ◽

THOMAS M. DOHERTY-BONE ◽

FABIO PUPIN ◽

DIEGO SAN MAURO ◽

...

Keyword(s):

Nuclear Gene ◽

Molecular Data ◽

Morphological Characterization ◽

Phylogenetic Position ◽

Nominal Species ◽

Phylogenetic Studies ◽

The Status ◽

Molecular Phylogenetic ◽

New Material ◽

Morphological And Molecular Data

Eight new specimens are reported for the caecilian amphibian Boulengerula fischeri, a species known in detail previously only from its holotype collected in 1987. The new material was collected at the type locality in Rwanda in 2009, and is used to expand and refine the morphological characterization of the species. Mitochondrial and nuclear gene sequences were used to assess the phylogenetic position of B. fischeri in the context of a recent molecular phylogeny of six of the seven other species of Boulengerula (from Kenya and Tanzania). Among nominal species, only B. denhardti remains to be included in molecular phylogenetic studies of Boulengerula. Boulengerula is recovered as monophyletic, with either B. fischeri or (more probably) B. boulengeri sister to all other sampled species. There are at least three deep lineages within Boulengerula: (1) B. boulengeri, (2) B. fischeri, and (3) all other Eastern Arc Mountain and Coastal Forest species from Kenya and Tanzania. The status of Afrocaecilia, a genus erected by Taylor in 1968 to contain all Boulengerula except B. boulengeri, is not yet resolved.

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Morphological and molecular data of two species of the rare genera Thada Thorne, 1941 and Tenunemellus Siddiqi, 1986 (Nematoda: Tylenchidae) from Iran

Journal of Helminthology ◽

10.1017/s0022149x20000279 ◽

2020 ◽

Vol 94 ◽

Author(s):

M. Hosseinvand ◽

A. Eskandari ◽

R. Ghaderi ◽

A. Karegar

Keyword(s):

New Species ◽

Ribosomal Dna ◽

Large Subunit ◽

Populus Euphratica ◽

Molecular Data ◽

Small Subunit ◽

Phylogenetic Studies ◽

Molecular Phylogenetic ◽

Morphological Differences ◽

Morphological And Molecular Data

Abstract Thada populus n. sp. was found in the rhizosphere of Populus euphratica in the city of Dezful, south-western Iran. The new species is characterized by its 365–453 μm body length, cuticle with transverse annuli 0.9–1.2 μm wide, lateral fields with four lines, lip region low with one or two annuli, 7.0–8.5 μm wide at base, longitudinal or slightly sigmoid amphidial slit, delicate stylet 8.4–9.8 μm long with posteriorly directed knobs, dorsal pharyngeal gland opening at 1.0–1.5 μm posterior to the stylet knobs, non-muscular and valve-less median bulb, pyriform and offset basal bulb, almost oval spermatheca, posterior position of vulva (V = 75–79%) without lateral membrane, short post-vulval uterine sac and conical tail with finely to broadly rounded terminus. Morphological differences of the new species with those of the type species, Thada striata, are discussed. Molecular phylogenetic studies of the new species using partial sequences of small subunit ribosomal DNA revealed that the new species formed a clade with Malenchus spp., Filenchus facultativus, F. fungivorus and Filenchus sp. in Bayesian inference. Morphological and molecular phylogenetic studies were also performed on Tenunemellus indicus, the second recovered species, the latter analysis using partial sequences of large subunit ribosomal DNA. In the inferred Bayesian tree, T. indicus formed a clade with Labrys fuzhouensis, Lelenchus leptosoma from the Netherlands, Malenchus spp. and Filenchus discrepans.

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Morphological and molecular characterization of an enigmatic clinostomid trematode (Digenea: Clinostomidae) parasitic as metacercariae in the body cavity of freshwater fishes (Cichlidae) across Middle America

Journal of Helminthology ◽

10.1017/s0022149x18000445 ◽

2018 ◽

Vol 93 (04) ◽

pp. 461-474 ◽

Cited By ~ 5

Author(s):

R. Briosio-Aguilar ◽

M. García-Varela ◽

D.I Hernández-Mena ◽

M. Rubio-Godoy ◽

G. Pérez-Ponce de León

Keyword(s):

Phylogenetic Relationships ◽

Freshwater Fishes ◽

Molecular Data ◽

Body Cavity ◽

The Body ◽

Valid Species ◽

The Family ◽

Middle America ◽

Molecular Phylogenetic ◽

Morphological And Molecular Data

AbstractThe family Clinostomidae Lühe, 1901 contains 29 species allocated to seven genera, of whichClinostomumLeidy, 1856 is the most diverse, withc.14 valid species. The diversity ofClinostomumhas been assessed, combining morphological and molecular data. The genetic library for species in this genus has increased steadily, although there is little or no information for the other genera included in the family. Molecular phylogenetic relationships among the genera of clinostomids have not been assessed, and their classification is still based on morphological traits. The monotypicIthyoclinostomumwas described from a fish-eating bird in Brazil, and its metacercariae have been found in several locations in South America, parasitizing erythrinid freshwater fishes. We collected unusually large metacercariae from the body cavity of cichlids in several locations across Middle America. These metacercariae exhibited some resemblance toIthyoclinostomum, although several differences prevent their inclusion inIthyoclinostomum dimorphum, casting doubt on their taxonomic identification. The main objective of this paper was to characterize the metacercariae collected in cichlids using both morphology and molecular data from three molecular markers, and to assess the molecular phylogenetic relationships among the genera of Clinostomidae to establish the position of the newly generated sequences. We took a conservative position and tentatively placed the metacercariae as belonging toIthyoclinostomum.

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Two new combinations in the genus Panus (Panaceae, Polyporales) based on morphology and molecular phylogeny

Phytotaxa ◽

10.11646/phytotaxa.514.3.8 ◽

2021 ◽

Vol 514 (3) ◽

pp. 287-294

Author(s):

VINJUSHA N. ◽

T. K. ARUN KUMAR

Keyword(s):

Molecular Phylogeny ◽

Molecular Data ◽

New Combinations ◽

Phylogenetic Studies ◽

Molecular Phylogenetic ◽

Morphological And Molecular Data ◽

Taxonomic Studies

Panus is a genus of the order Polyporales with a gilled hymenium. Although Panus was earlier considered as a sub genus of the genus Lentinus, molecular phylogenetic studies support the independent status of the genus. As a result, many species were transferred from Lentinus to Panus. Many more species of Lentinus that are more related to Panus, still await critical taxonomic studies and formal transfers. Morphological and molecular data obtained from L. bambusinus and L. roseus reveal that these are Panus species. Two new combinations in the genus Panus are proposed here.

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Redescription of Paradiplozoon hemiculteri (Monogenea, Diplozoidae) from the type host Hemiculter leucisculus, with neotype designation

Parasite ◽

10.1051/parasite/2018004 ◽

2018 ◽

Vol 25 ◽

pp. 4 ◽

Cited By ~ 3

Author(s):

Dagmar Jirsová ◽

Xuejuan Ding ◽

Kristína Civáňová ◽

Eliška Jirounková ◽

Jana Ilgová ◽

...

Keyword(s):

Southern China ◽

Phylogenetic Analyses ◽

Posterior Part ◽

Original Description ◽

Molecular Data ◽

The Body ◽

Type Host ◽

Hemiculter Leucisculus ◽

Morphological And Molecular Data ◽

Median Plate

Paradiplozoon hemiculteri (Ling, 1973), a member of the Diplozoidae, parasitizes the gills of Asian fish. Not only is the type material unavailable for this species, the original description was poor and somewhat conflicting, and adequate molecular data were not available. What is more, the available morphological and molecular data are inconsistent and fluctuate significantly. Here, we present a redescription of P. hemiculteri based on morphological and molecular data from new isolates collected from the type host, the sharpbelly Hemiculter leucisculus (Basilewsky, 1855), captured at the neotype locality (Shaoguan, Guangdong Province, southern China); a neotype for P. hemiculteri was designated from this collection. The length and width of the body, buccal suckers, pharynx, attachment clamps, sickle and the central hook handle were all measured and the shape of the anterior and posterior part of the median plate and anterior and posterior joining sclerites accurately documented. Phylogenetic analyses based on the sequences of the second rDNA internal transcribed spacer (ITS2) indicated that all new samples clustered together and differed clearly from sequences attributed to P. hemiculteri, which are deposited in GenBank. Our results confirm that P. hemiculteri is the only diplozoid that has demonstrably been found on the gills of H. leucisculus to date.

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Aymoreana (Nelsonioideae, Acanthaceae), a New Genus Endemic to Brazil

Systematic Botany ◽

10.1600/036364421x16128061189530 ◽

2021 ◽

Vol 46 (1) ◽

pp. 211-217

Author(s):

Denise Monte Braz ◽

Thomas F. Daniel ◽

Carrie Kiel ◽

Anna Gao ◽

Sagrika Jawadi ◽

...

Keyword(s):

New Genus ◽

Molecular Data ◽

Ecological Data ◽

Conservation Assessment ◽

Eastern Brazil ◽

Molecular Phylogenetic Tree ◽

Molecular Phylogenetic ◽

Southern Bahia ◽

Previously Treated ◽

Morphological And Molecular Data

Abstract—A species previously treated in Staurogyne (S. nitida) is elevated to the category of a new genus of Acanthaceae, subfamily Nelsonioideae, based on morphological and molecular data. The sole species, Aymoreana nitida, occurs in the Atlantic Forest of eastern Brazil, from southern Bahia to northern Espírito Santo. Aymoreana differs from other genera of Nelsonioideae by the combination of the calyx with subequal segments, the slightly zygomorphic corolla, the four didynamous stamens, and the asymmetric gynoecium. Morphological information is accompanied by a molecular phylogenetic tree, ecological data, a preliminary conservation assessment, and illustrations.

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Morphological studies and molecular data on a new marine ciliate, Apokeronopsis sinica n. sp. (Ciliophora: Urostylida), from the South China Sea

Zootaxa ◽

10.11646/zootaxa.2005.1.5 ◽

2009 ◽

Vol 2005 (1) ◽

pp. 57-66 ◽

Cited By ~ 5

Author(s):

WEIWEI LIU ◽

JIQIU LI ◽

SHAN GAO ◽

CHEN SHAO ◽

JUN GONG ◽

...

Keyword(s):

Molecular Data ◽

Small Subunit ◽

Rrna Gene ◽

Small Subunit Rrna ◽

Cortical Granules ◽

Marine Ciliate ◽

Morphological Studies ◽

Small Subunit Rrna Gene ◽

Morphological And Molecular Data

The morphology of a new marine urostylid ciliate, Apokeronopsis sinica n. sp., collected from the Clear Water Bay, Hong Kong, was investigated. Additionally, the SSrRNA gene was sequenced in order to make a comparison at molecular level. Based on both morphological and molecular data, descriptions and comparisons with its congeners are provided. Apokeronopsis sinica is characterized by: body size about 150–200 × 50–65 µm in vivo; two kinds of cortical granules; about 20 cirri in frontal area which form the non-typical bicorona; 2 frontoterminal, ca. 4 buccal and 10 transverse cirri; midventral complex consists of 21–32 pairs of cirri; on average 35 right and 30 left marginal cirri; about 50 membranelles; invariably 3 dorsal kineties. A key to the identification of the known Apokeronopsis species was suggested. The small subunit rRNA gene sequence differences between Apokeronopsis sinica and its congeners range from 1.64% to 3.72%.

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