The systematics of Boulengerula fischeri (Amphibia: Gymnophiona: Caeciliidae) based on morphological and molecular data

Zootaxa ◽  
2011 ◽  
Vol 2767 (1) ◽  
pp. 14 ◽  
Author(s):  
DAVID J. GOWER ◽  
ANNA PAPADOPOULOU ◽  
THOMAS M. DOHERTY-BONE ◽  
FABIO PUPIN ◽  
DIEGO SAN MAURO ◽  
...  

Eight new specimens are reported for the caecilian amphibian Boulengerula fischeri, a species known in detail previously only from its holotype collected in 1987. The new material was collected at the type locality in Rwanda in 2009, and is used to expand and refine the morphological characterization of the species. Mitochondrial and nuclear gene sequences were used to assess the phylogenetic position of B. fischeri in the context of a recent molecular phylogeny of six of the seven other species of Boulengerula (from Kenya and Tanzania). Among nominal species, only B. denhardti remains to be included in molecular phylogenetic studies of Boulengerula. Boulengerula is recovered as monophyletic, with either B. fischeri or (more probably) B. boulengeri sister to all other sampled species. There are at least three deep lineages within Boulengerula: (1) B. boulengeri, (2) B. fischeri, and (3) all other Eastern Arc Mountain and Coastal Forest species from Kenya and Tanzania. The status of Afrocaecilia, a genus erected by Taylor in 1968 to contain all Boulengerula except B. boulengeri, is not yet resolved.

2006 ◽  
Vol 51 (4) ◽  
Author(s):  
Stephen Curran ◽  
Vasyl Tkach ◽  
Robin Overstreet

AbstractThe type material of Polylekithum ictaluri, P. halli, and Maculifer chandleri was examined from the United States National Parasite Museum, and we determined that the material was conspecific, making P. halli and M. chandleri junior subjective synonyms of P. ictaluri. Polylekithum catahoulensis sp. nov. was described from material collected from catfishes at the Catahoula Wildlife Refuge, LaSalle Parish, Louisiana, USA, and compared with P. ictaluri collected from catfishes in Reelfoot Lake, Obion County, Tennessee, USA, and the Pearl River, Hancock County, Mississippi, USA. Polylekithum catahoulensis had smaller eggs (77–88 μm long by 51–63 μm wide vs. 94–108 μm by 52–76 μm) and a longer forebody (35–41% of overall body length vs. 29–34%). Comparison of more than 2,400 bp long fragments of nuclear ribosomal DNA (complete ITS and partial 28S regions) strongly supported the status of P. catahoulensis as a new species. Molecular phylogenetic analysis of 28S rDNA gene sequences from Polylekithum as well as representative species from Allocreadiidae, Atractotrematidae, Brachycoeliidae, Callodistomidae, Dicrocoeliidae, Encyclometridae, Gorgoderidae, Haploporidae, Opecoelidae, Plagiorchiidae, and Telorchiidae rooted by Monorchiidae and Lissorchiidae demonstrated that of the families tested, Polylekithum was most closely related to Encyclometridae as a gorgoderoid and not to Allocreadiidae as previously reported. Morphological features of three South American allocreadiids, Allocreadium patagonicum, P. percai, and A. pichi were inconsistent with generic diagnoses of Allocreadium and Polylekithum, so we suggested they belonged in a single unnamed genus similar to Creptotrema. Polylekithum catlai from India was assessed from the description and failed to conform to the generic diagnosis of Polylekithum. Morphology of Caudouterina suggested a close relationship with Polylekithum and not Allocreadiidae.


Biologia ◽  
2011 ◽  
Vol 66 (4) ◽  
Author(s):  
Christina Bock ◽  
Marie Pažoutová ◽  
Lothar Krienitz

AbstractFollowing traditional morphological concepts, the genus Coronastrum is considered to be a rare member of the Scenedesmaceae (Chorophyceae). This classification may be called into question when molecular data are taken into account as well. Recent molecular phylogenetic studies revealed the polyphyletic origin of the family Scenedesmaceae within the Chlorophyceae and Trebouxiophyceae. In a combined approach of morphological analyses, SSU/ITS rRNA gene phylogeny and comparison of the ITS secondary structure, we analysed the systematics of Coronastrum strains available in public strain collections. Our molecular analyses revealed a new subclade within the Chlorella clade of the Chlorellaceae consisting of Coronastrum ellipsoideum, two strains with Dictyosphaerium-like morphology and one strain which fits the description of the genus Parachlorella. Four additional strains formed together a new lineage within the genus Parachlorella in the Parachlorella clade of the Chlorellaceae. These strains differ from the already known Parachlorella species in complementary base changes within the ITS2 and are here described for the first time as Parachlorella hussii sp. nov.


2019 ◽  
Vol 94 ◽  
Author(s):  
F. Heydari ◽  
F. Gharibzadeh ◽  
E. Pourjam ◽  
M. Pedram

Abstract This contribution provides morphological and molecular data for one new and one known species of the genus Pungentus. The first species, P. azarbaijanensis n. sp., was recovered from the rhizospheric soil of grasses, collected in West Azarbaijan province, Iran, and was characterized by 2082–2365 μm long females having an angular lip region separated from the rest of the body by a constriction, 33–35 μm long odontostyle, vulva at 43.5–51.0%, 27.0–29.5 μm long rounded-conoid tail, and males unknown. It was compared morphologically with five species: P. angulosus, P. crassus, P. marietani, P. parapungens and P. pungens, which have didelphic-amphidelphic female reproductive system, body longer than 1.5 mm, and odontostyle longer than 20 μm. The second species, P. engadinensis, was recovered in three different regions of Iran (Mazandaran, Semnan and East Azarbaijan provinces). It was compared morphologically with some other populations reported all over the world. Besides morphological studies, molecular phylogenetic studies using partial sequences of 28S rDNA D2-D3 fragments were performed, and the phylogenetic relations of the two Iranian populations with other species and genera were discussed.


2017 ◽  
Vol 57 (3) ◽  
pp. 248-255 ◽  
Author(s):  
Ahmad Valizadeh ◽  
Shila Goldasteh ◽  
Zahra Rafiei-Karahroodi ◽  
Majid Pedram

AbstractDue to importance and effectiveness of some entomopathogenic or insect parasitic nematodes in controlling of agricultural pests, or pests of non-agricultural plants, a study was conducted in order to identify the species of this group of nematodes in city of Tehran. As the result, three species belonging to the genusOscheiuswere recovered in association with bark samples having the bark beetle galleries. Morphological and molecular data were provided for two recently recovered species of the genus, namelyO. necromenusandO. onirici. Molecular data were also provided for a recently recovered isolate ofO. tipulae. All three species were recovered in association with bark samples collected from dead trees in the city of Tehran. Morphological characters and morphometric data of the two aforementioned species are in accordance with the data given in their original descriptions. One recovered individual from a small bark sample characterized by its short body length was sequenced for its 28S and internal transcribed spacer (ITS) rDNA loci, and the results of BLAST search using the newly obtained partial sequences revealed that it belonged toO. tipulae. Molecular phylogenetic studies revealed recently sequenced Iranian populations ofO. oniriciandO. tipulaeforming a clade with other isolates/populations of these species in ITS tree with maximal Bayesian posterior probability (BPP), and presently sequenced isolates ofO. tipulaeandO. necromenusform a clade with other isolates of these species in 28S tree. The two speciesO. oniriciandO. necromenuswere reported in Iran for the first time.


2019 ◽  
Vol 94 ◽  
Author(s):  
S.V. Shchenkov ◽  
S.A. Denisova ◽  
G.A. Kremnev ◽  
A.A. Dobrovolskij

Abstract The phylogenetic position of most xiphidiocercariae from subgroups Cercariae virgulae and Cercariae microcotylae remains unknown or unclear, even at the family level. In this paper, we studied the morphology and molecular phylogeny of 15 microcotylous and virgulate cercariae (11 new and four previously described ones). Based on morphological and molecular data, we suggested five distinct morphological types of xiphidiocercariae, which are a practical alternative to Cercariae virgulae and Cercariae microcotylae subgroups. Four of these types correspond to actual digenean taxa (Microphallidae, Lecithodendriidae, Pleurogenidae and Prosthogonimidae), while the fifth is represented by Cercaria nigrospora Wergun, 1957, which we classified on the basis of molecular data for the first time. We reassessed the relative importance of morphological characters used for the classification of virgulate and microcotylous cercariae, and discussed the main evolutionary trends within xiphidiocercariae. Now stylet cercariae can be reliably placed into several sub-taxa of Microphalloidea on the basis of their morphological features.


Phytotaxa ◽  
2017 ◽  
Vol 311 (3) ◽  
pp. 270 ◽  
Author(s):  
LIN ZHU ◽  
JIA-HUI XING ◽  
BAO-KAI CUI

Based on phylogenetic studies, the Inonotus linteus complex has been recently divided into two genera, Sanghuangporus and Tropicoporus. During investigations on the species diversity of the Inonotus linteus complex from China, a new species, Sanghuangporus quercicola sp. nov., is described based on morphological and molecular data. Morphologically, it is characterized by perennial, pileate basidiomata, a heterogeneous hyphal system with monomitic in context and dimitic in trama, and broadly subglobose to ovoid, thick-walled basidiospores measuring as 3–3.9 × 2.4–2.8 μm. Phylogenetically, the status of S. quercicola is strongly supported based on sequences of the nuclear internal transcribed spacer (ITS) regions.


2020 ◽  
Vol 94 ◽  
Author(s):  
M. Hosseinvand ◽  
A. Eskandari ◽  
R. Ghaderi ◽  
A. Karegar

Abstract Thada populus n. sp. was found in the rhizosphere of Populus euphratica in the city of Dezful, south-western Iran. The new species is characterized by its 365–453 μm body length, cuticle with transverse annuli 0.9–1.2 μm wide, lateral fields with four lines, lip region low with one or two annuli, 7.0–8.5 μm wide at base, longitudinal or slightly sigmoid amphidial slit, delicate stylet 8.4–9.8 μm long with posteriorly directed knobs, dorsal pharyngeal gland opening at 1.0–1.5 μm posterior to the stylet knobs, non-muscular and valve-less median bulb, pyriform and offset basal bulb, almost oval spermatheca, posterior position of vulva (V = 75–79%) without lateral membrane, short post-vulval uterine sac and conical tail with finely to broadly rounded terminus. Morphological differences of the new species with those of the type species, Thada striata, are discussed. Molecular phylogenetic studies of the new species using partial sequences of small subunit ribosomal DNA revealed that the new species formed a clade with Malenchus spp., Filenchus facultativus, F. fungivorus and Filenchus sp. in Bayesian inference. Morphological and molecular phylogenetic studies were also performed on Tenunemellus indicus, the second recovered species, the latter analysis using partial sequences of large subunit ribosomal DNA. In the inferred Bayesian tree, T. indicus formed a clade with Labrys fuzhouensis, Lelenchus leptosoma from the Netherlands, Malenchus spp. and Filenchus discrepans.


Phytotaxa ◽  
2014 ◽  
Vol 178 (4) ◽  
pp. 287
Author(s):  
Mehmet Fırat ◽  
Barış Özüdoğru ◽  
Burcu Tarıkahya Hacıoğlu ◽  
Ali Savaş Bülbül ◽  
Ihsan Ali Al-Shehbaz ◽  
...  

A general review of the taxonomic status of Thlaspi past and present is given, and a critical evaluations of its segregates based on both morphological and molecular data are  presented. ITS molecular phylogenetic study of Thlaspi aghricum and related species, as well as seed-coat morphology and anatomy strongly support the placement of the species in Noccaea. The new combination N. aghrica is proposed, and detailed description and distribution of the species are given.


Phytotaxa ◽  
2021 ◽  
Vol 514 (3) ◽  
pp. 287-294
Author(s):  
VINJUSHA N. ◽  
T. K. ARUN KUMAR

Panus is a genus of the order Polyporales with a gilled hymenium. Although Panus was earlier considered as a sub genus of the genus Lentinus, molecular phylogenetic studies support the independent status of the genus. As a result, many species were transferred from Lentinus to Panus. Many more species of Lentinus that are more related to Panus, still await critical taxonomic studies and formal transfers. Morphological and molecular data obtained from L. bambusinus and L. roseus reveal that these are Panus species. Two new combinations in the genus Panus are proposed here.


Phytotaxa ◽  
2018 ◽  
Vol 357 (3) ◽  
pp. 189
Author(s):  
ZHI-JIAN YIN ◽  
NORBERT KILIAN ◽  
BING-ZHANG LI ◽  
HONG-YAN SUN ◽  
MING-XU ZHAO ◽  
...  

Melanoseris jilongensis from SW Xizang, China, is described as a species new to science and illustrated, and its threat status is also assessed. It morphologically resembles M. macrorhiza, but differs in particular in leaf morphology, indumentum and achene characters. Molecular phylogenetic analysis based on nrITS and plastid DNA sequences revealed discordance between both DNA datasets as well as between morphological similarity and phylogenetic position. As in other cases of Sino-Himalayan taxa of the Melanoseris lineage, an event of reticulation in the speciation of M. jilongensis seems to have been taken place.


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