scholarly journals Morphological and molecular characterization of Aporcella femina sp. n. (Dorylaimida, Aporcelaimidae) from Nigeria

2021 ◽  
Vol 95 ◽  
Author(s):  
M. Rashidifard ◽  
T.T. Bello ◽  
H. Fourie ◽  
D.L. Coyne ◽  
R. Peña-Santiago
Keyword(s):  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Small Subunit ◽  
Female Genital System ◽  
Close Relationship ◽  
Relationship Of ◽  
A New Species ◽  
Morphological And Molecular Characterization ◽  
Female Genital

Abstract A new species of the genus Aporcella collected from a watermelon field in Nigeria is described, including its morphological and molecular (small subunit (SSU) and large subunit (LSU) ribosomal DNA (rDNA)) characterization. Aporcella femina sp. n. is distinguished by its 3.21–3.64 mm-long body, inner cuticle layer with fine but distinct transverse striation, lip region offset by deep constriction, 22–25 μm broad, odontostyle 20–26 μm, neck 661–811 μm long, pharyngeal expansion occupying 52–56% of the total neck length, female genital system didelphic–amphidelphic, uterus 191–350 μm or 1.9–3.3 mid-body diameters long, V = 52–57, tail short and convex conoid (35–48 μm, c = 72–98, c′ = 0.7–0.9) and males absent. Phylogenetic analyses based on the partial sequence of SSU and LSU (D2–D3) rDNA revealed a close relationship of A. femina sp. n. with other Aporcella species, confirming the monophyly of the genus as well as its association to a clade made of several taxa characterized by the absence of pars refringens vaginae.

scholarly journals Morphology, genetic characterization and molecular phylogeny of pinworm Skrjabinema longicaudatum n. sp. (Oxyurida: Oxyuridae) from the endangered Tibetan antelope Pantholops hodgsonii (Abel) (Artiodactyla: Bovidae)

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Yi-Fan Cao ◽  
Hui-Xia Chen ◽  
Yang Li ◽  
Dang-Wei Zhou ◽  
Shi-Long Chen ◽  
...  
Keyword(s):  
New Species ◽  
Ribosomal Dna ◽  
Dna Sequences ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Small Subunit ◽  
Sister Relationship ◽  
Tibetan Antelope ◽  
A New Species

Abstract Background The Tibetan antelope Pantholops hodgsonii (Abel) (Artiodactyla: Bovidae) is an endangered species of mammal endemic to the Qinghai-Tibetan Plateau. Parasites and parasitic diseases are considered to be important threats in the conservation of the Tibetan antelope. However, our present knowledge of the composition of the parasites of the Tibetan antelope remains limited. Methods Large numbers of nematode parasites were collected from a dead Tibetan antelope. The morphology of these nematode specimens was observed using light and scanning electron microscopy. The nuclear and mitochondrial DNA sequences, i.e. small subunit ribosomal DNA (18S), large subunit ribosomal DNA (28S), internal transcribed spacer (ITS) and cytochrome c oxidase subunit 1 (cox1), were amplified and sequenced for molecular identification. Moreover, phylogenetic analyses were performed using maximum likelihood (ML) inference based on 28S and 18S + 28S + cox1 sequence data, respectively, in order to clarify the systematic status of these nematodes. Results Integrated morphological and genetic evidence reveals these nematode specimens to be a new species of pinworm Skrjabinema longicaudatum (Oxyurida: Oxyuridae). There was no intraspecific nucleotide variation between different individuals of S. longicaudatum n. sp. in the partial 18S, 28S, ITS and cox1 sequences. However, a high level of nucleotide divergence was revealed between the new species and its congeners in 28S (8.36%) and ITS (20.3–23.7%) regions, respectively. Molecular phylogenetic results suggest that the genus Skrjabinema should belong to the subfamily Oxyurinae (Oxyuroidea: Oxyuridae), instead of the subfamily Syphaciidae or Skrjabinemiinae in the traditional classification, as it formed a sister relationship to the genus Oxyuris. Conclusions A new species of pinworm Skrjabinema longicaudatum n. sp. (Oxyurida: Oxyuridae) is described. Skrjabinema longicaudatum n. sp. represents the first species of Oxyurida (pinworm) and the fourth nematode species reported from the Tibetan antelope. Our results contribute to the knowledge of the species diversity of parasites from the Tibetan antelope, and clarify the systematic position of the genus Skrjabinema.

Morphological and molecular data on a new species of Plagiorhynchus Lühe, 1911 (Acanthocephala: Plagiorhynchidae) from the long-billed curlew (Numenius americanus) from northern Mexico

2019 ◽  
Vol 94 ◽  
Author(s):  
M. García-Varela ◽  
J.-K. Park ◽  
J.S. Hernández-Orts ◽  
C.D. Pinacho-Pinacho
Keyword(s):  
New Species ◽  
Marine Mammals ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Molecular Data ◽  
Small Subunit ◽  
Nuclear Ribosomal Dna ◽  
Northern Mexico ◽  
Numenius Americanus ◽  
A New Species

Abstract A new species of the genus Plagiorhynchus Lühe, 1911 from the intestine of the long-billed curlew (Numenius americanus) from northern Mexico is described. Plagiorhynchus (Plagiorhynchus) aznari n. sp. is morphologically distinguished from other congeneric species from the Americas by having a trunk expanded anteriorly and a cylindrical proboscis, armed with 19 longitudinal rows of hooks, with 14–15 hooks each row. Nearly complete sequences of the small subunit and large subunit of the nuclear ribosomal DNA of the new species were determined and compared with available sequences from GenBank. Phylogenetic analyses inferred from the two molecular markers consistently showed that P. (Plagiorhynchus) aznari n. sp. is closely related to P. (Plagiorhynchus) allisonae, and this clade is sister to a clade formed by P. (Prosthorhynchus) transversus and P. (Prosthorhynchus) cylindraceus from Plagiorhynchidae. The new species represents the second record of the genus in Mexico and the fourth species in the Americas. The phylogenetic relationships among the members of the order Polymorphida in this study provide significant insights into the evolution of ecological associations between parasites and their definitive hosts. Our analyses suggest that the colonization of marine mammals, fish-eating birds and waterfowl in Polymorphidae might have occurred independently, from a common ancestor of Centrorhynchidae and Plagiorhynchidae that colonized terrestrial birds and mammals.

Morphological and molecular characterization of Lenonchium zanjanense sp. n. (Dorylaimida, Nordiidae) from Iran, with new insights into the phylogeny of the genus and a revision of its taxonomy

2019 ◽  
Vol 94 ◽  
Author(s):  
M. Asgari ◽  
A. Eskandari ◽  
H.M. Karani ◽  
J. Abolafia ◽  
R. Peña-Santiago
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Natural Habitat ◽  
Genital System ◽  
Female Genital System ◽  
Morphological And Molecular Characterization ◽  
Scanning Electron ◽  
Female Genital

Abstract The new species Lenonchium zanjanense sp. n. is described from a natural habitat of Zanjan province, Iran, including line, light microscopy and scanning electron microscopy illustrations and a molecular (18S, 28S) study. It is characterized by its 3.50–4.51 mm long body, rounded lip region, continuous and 13.5–15.5 µm broad, odontostyle 21–24 µm long, neck 362–490 µm long, double guiding ring, pharyngeal expansion 190–285 µm long, female genital system didelphic–amphidelphic, uterus simple and 185–320 µm long or 3.4–5.9 times the corresponding body diameter, vulva nearly equatorial (V = 45–53), tail conical-elongated to filiform (90–165 µm, c = 23–43, c′ = 2.4–5.3) with three or four mucro-like projections at the tip, spicules 58–64 µm long and 16–21 contiguous ventromedian supplements ending at the level of the anterior end of the spicules. The taxonomy of the genus is updated, with an emended diagnosis, list of species, key to their identification and a compendium of their main morphometrics. Lenonchium asterocaudatum is regarded as identical and a junior synonym of L. denticaudatum. New insights into the phylogeny of the group are also provided, and the classification of Lenonchium within Nordiidae is seriously questioned.

Filsoniana lhasanensis sp. nov. from Tibet, China

Mycotaxon ◽  
2020 ◽  
Vol 134 (4) ◽  
pp. 663-675
Author(s):  
Xue-Mei Wen ◽  
Hurnisa Shahidin ◽  
Abdulla Abbas
Keyword(s):  
New Species ◽  
Internal Transcribed Spacer ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Small Subunit ◽  
Dark Orange ◽  
A New Species

A new species, Filsoniana lhasanensis, is described from Tibet, China, where it grows on rocks at altitudes 4200–4432 m. The lichen is characterised by a poorly developed or absent dark orange areolate thallus, lecanorine to zeorine orange to deep-orange aggregated apothecia, and richly branched and anastomosed paraphyses. DNA was obtained for three gene loci—nuclear internal transcribed spacer (ITS), nuclear large subunit (LSU), and mitochondrial small subunit (SSU). Phylogenetic analyses support the taxon as a new species of Filsoniana .

Morphological and molecular characterization of Hemicycliophora subbotini n. sp. (Tylenchida: Hemicycliophoridae) from China

Zootaxa ◽  
2018 ◽  
Vol 4433 (1) ◽  
pp. 161 ◽  
Author(s):  
MUNAWAR MARIA ◽  
RUIHANG CAI ◽  
NAN QU ◽  
PABLO CASTILLO ◽  
JINGWU ZHENG
Keyword(s):  
New Species ◽  
Phylogenetic Analyses ◽  
Oral Disc ◽  
Pharyngeal Bulb ◽  
En Face ◽  
The Status ◽  
A New Species ◽  
Morphological And Molecular Characterization ◽  
Lateral Plates

Sheath nematodes of the genus Hemicycliophora are migratory root-ectoparasites of many crops, but the damage is documented for only a few species. Hemicycliophora subbotini n. sp., isolated in Hangzhou, Zhejiang, China, from the rhizosphere of camphor tree (Cinnamomum camphora (L.) Presl), is described and characterized morphologically and molecularly with important morphological details elucidated by SEM studies. The new species can be characterized by the conoid lip region, with bulging oral disc protruding beyond the first annulus in some specimens. Oral disc narrow ovate, amphidial apertures covered by lateral plates, lying in the same plane as oral disc when observed en face. Stylet 118 (108–125) μm long and flexible, stylet knobs concave shaped having a slight cavity. The excretory pore is located 1–2 annuli posterior to the base of pharyngeal bulb. Vulval lips slightly modified with small vulval sleeve, spermatheca rounded to oblong. Anus located 18 (15–22) annuli posterior to vulva. Tail elongated, with conoid or slightly offset spike having a narrowly rounded terminus. The study provides the first description of a new Hemicycliophora species from China. Phylogenetic analyses based on D2–D3 expansion segments of the 28S and ITS fragments revealed the new species forming a separate clade from other Hemicycliophora species which supported the status as a new species of this genus. 

Latronema whataitai sp. n. (Nematoda: Selachinematidae) from intertidal sediments of New Zealand, with notes on relationships within the family based on preliminary 18S and D2-D3 phylogenetic analyses

Nematology ◽  
2015 ◽  
Vol 17 (8) ◽  
pp. 941-952 ◽  
Author(s):  
Daniel Leduc ◽  
Zeng Zhao
Keyword(s):  
New Zealand ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Small Subunit ◽  
Rrna Genes ◽  
Intertidal Sediments ◽  
Molecular Sequences ◽  
Close Relationship ◽  
Central Tooth ◽  
Family Based

Latronema whataitai sp. n. is described from intertidal sediments of Hataitai Beach, North Island of New Zealand, and its placement within the Selachinematidae investigated using 18S and D2-D3 molecular sequences. Latronema whataitai sp. n. is characterised by relatively slender body (a = 22-25), outer labial setae and cephalic setae of similar length, anterior buccal cavity with three sets of seven teeth with larger central tooth positioned above the other six, circular amphid with faint outline, males with 12-13 precloacal supplements and spicules with pointed projection near distal end, and tail 2.0-2.6 anal body diam. long. The cuticle ultrastructure of the genus is investigated for the first time using scanning electron microscopy. Molecular phylogenies of near full length small subunit and D2-D3 expansion segments of the large subunit rRNA genes do not provide support for the division of Selachinematidae into the subfamilies Selachinematinae and Choniolaiminae, and do not support a close relationship between Latronema and Richtersia despite some morphological similarities. These preliminary results are based on a small number of molecular sequences, and therefore the current morphology-based classification remains the most tractable system for the Selachinematidae until more comprehensive analyses are conducted.

Molecular phylogeny of two coelomycetous fungal genera with stellate conidia,ProsthemiumandAsterosporium, on Fagales trees

Botany ◽  
2010 ◽  
Vol 88 (12) ◽  
pp. 1057-1071 ◽  
Author(s):  
Kazuaki Tanaka ◽  
Vadim A. Mel’nik ◽  
Maasa Kamiyama ◽  
Kazuyuki Hirayama ◽  
Takashi Shirouzu
Keyword(s):  
Internal Transcribed Spacer ◽  
Large Subunit ◽  
Diagnostic Value ◽  
Small Subunit ◽  
Nuclear Ribosomal Dna ◽  
Internal Transcribed Spacer Region ◽  
Phylogenetic Relatedness ◽  
Close Relationship ◽  
Phylogenetic Affinities ◽  
A New Species

Prosthemium (teleomorph Pleomassaria ) and Asterosporium (teleomorph unknown) are coelomycetous genera with stellate conidia on Fagales trees. Their morphological resemblance suggests their close relationship, but phylogenetic relatedness remains unknown. They have been distinguished on the basis of either conidiomatal morphology (pycnidia in Prosthemium and acervuli in Asterosporium) or their differing conidial septation (euseptate in Prosthemium and distoseptate in Asterosporium). To reveal their phylogenetic affinities and clarify reliable distinguishing phenotypical characters, five species of Prosthemium and two species of Asterosporium were investigated using sequences of the small subunit, large subunit, and internal transcribed spacer region of nuclear ribosomal DNA and β-tubulin gene from 43 isolates of these species. The analyses revealed the following: (i) Asterosporium typified by Asterosporium asterospermum on Fagus is a member of the Sordariomycetes and is distinct from Prosthemium belonging to the Dothideomycetes; (ii) Asterosporium betulinum on Betula species should be excluded from Asterosporium and transferred to Prosthemium as Prosthemium neobetulinum nom. nov.; (iii) conidial septation does not seem to have a diagnostic value, whereas conidiomatal morphology is useful in distinguishing both genera; (iv) the number and length of conidial arms are useful criteria in distinguishing Prosthemium species. A new species, Prosthemium intermedium sp. nov., found on both Betula ermanii and Betula maximowicziana , is described and illustrated.

scholarly journals Morphology, genetic characterization and molecular phylogeny of pinworm Skrjabinema longicaudatum sp. n. (Oxyurida: Oxyuridae) from the endangered Tibetan antelope Pantholops hodgsonii (Abel) (Artiodactyla: Bovidae)

2020 ◽  
Author(s):  
Yi-Fan Cao ◽  
Hui-Xia Chen ◽  
Yang Li ◽  
Dang-Wei Zhou ◽  
Shi-Long Chen ◽  
...  
Keyword(s):  
New Species ◽  
Ribosomal Dna ◽  
Dna Sequences ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Small Subunit ◽  
Sister Relationship ◽  
Tibetan Antelope ◽  
A New Species

Abstract Background: The Tibetan antelope Pantholops hodgsonii (Abel) (Artiodactyla: Bovidae) is an endangered species of mammal endemic to the Qinghai-Tibetan Plateau. Parasites and parasitic diseases are considered to be important threats in the conservation of the Tibetan antelope. However, our present knowledge of the composition of the parasites from the Tibetan antelope remains limited. Methods: Large numbers of nematode parasites were collected from a dead Tibetan antelope. The morphology of these nematode specimens was observed using light and scanning electron microscopy. The nuclear and mitochondrial DNA sequences [i.e. small subunit ribosomal DNA (18S), large subunit ribosomal DNA (28S), internal transcribed spacer (ITS) and cytochrome c oxidase subunit 1 (cox1)] were amplified and sequenced for molecular identification. Moreover, phylogenetic analyses were performed using maximum likelihood (ML) inference based on 28S and 18S + 28S + cox1 sequence data, respectively, in order to clarify the systematic status of these nematodes.Results: Integrated morphological and genetic evidence reveals these nematode specimens to be a new species of pinworm Skrjabinema longicaudatum (Oxyurida: Oxyuridae). There was no intraspecific nucleotide variation between different individuals of S. longicaudatum sp. n. in the partial 18S, 28S, ITS and cox1 sequences. However, a high level of nucleotide divergence was revealed between the new species and its congeners in 28S (8.36%) and ITS (20.3–23.7%) regions, respectively. Molecular phylogenetic results suggest that the genus Skrjabinema should belong to the subfamily Oxyurinae (Oxyuroidea: Oxyuridae), instead of the subfamily Syphaciidae or Skrjabinemiinae in the traditional classification, as it formed a sister relationship to the genus Oxyuris. Conclusions: A new species of pinworm Skrjabinema longicaudatum sp. n. (Oxyurida: Oxyuridae) is described. Skrjabinema longicaudatum sp. n. represents the first species of Oxyurida (pinworm) and the fourth nematode species reported from the Tibetan antelope. Our results contribute to the knowledge of the species diversity of parasites from the Tibetan antelope, and clarify the systematic position of the genus Skrjabinema.

scholarly journals Morphology, genetic characterization and molecular phylogeny of pinworm Skrjabinema longicaudatum sp. n. (Oxyurida: Oxyuridae) from the endangered Tibetan antelope Pantholops hodgsonii (Abel) (Artiodactyla: Bovidae)

2020 ◽  
Author(s):  
Yi-Fan Cao ◽  
Hui-Xia Chen ◽  
Yang Li ◽  
Dang-Wei Zhou ◽  
Shi-Long Chen ◽  
...  
Keyword(s):  
New Species ◽  
Ribosomal Dna ◽  
Dna Sequences ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Small Subunit ◽  
Sister Relationship ◽  
Tibetan Antelope ◽  
A New Species

Abstract Background: The Tibetan antelope Pantholops hodgsonii (Abel) (Artiodactyla: Bovidae) is an endangered species of mammal endemic to the Qinghai-Tibetan Plateau. Parasites and parasitic diseases are considered to be important threats in the conservation of the Tibetan antelope. However, our present knowledge of the composition of the parasites from the Tibetan antelope remains limited. Methods: Large numbers of nematode parasites were collected from a dead Tibetan antelope. The morphology of these nematode specimens was observed using light and scanning electron microscopy. The nuclear and mitochondrial DNA sequences [i.e. small subunit ribosomal DNA (18S), large subunit ribosomal DNA (28S), internal transcribed spacer (ITS) and cytochrome c oxidase subunit 1 (cox1)] were amplified and sequenced for molecular identification. Moreover, phylogenetic analyses were performed using maximum likelihood (ML) inference based on 28S and 18S + 28S + cox1 sequence data, respectively, in order to clarify the systematic status of these nematodes.Results: Integrated morphological and genetic evidence reveals these nematode specimens to be a new species of pinworm Skrjabinema longicaudatum (Oxyurida: Oxyuridae). There was no intraspecific nucleotide variation between different individuals of S. longicaudatum sp. n. in the partial 18S, 28S, ITS and cox1 sequences. However, a high level of nucleotide divergence was revealed between the new species and its congeners in 28S (8.36%) and ITS (20.3–23.7%) regions, respectively. Molecular phylogenetic results suggest that the genus Skrjabinema should belong to the subfamily Oxyurinae (Oxyuroidea: Oxyuridae), instead of the subfamily Syphaciidae or Skrjabinemiinae in the traditional classification, as it formed a sister relationship to the genus Oxyuris. Conclusions: A new species of pinworm Skrjabinema longicaudatum sp. n. (Oxyurida: Oxyuridae) is described. Skrjabinema longicaudatum sp. n. represents the first species of Oxyurida (pinworm) and the fourth nematode species reported from the Tibetan antelope. Our results contribute to the knowledge of the species diversity of parasites from the Tibetan antelope, and clarify the systematic position of the genus Skrjabinema.

scholarly journals Morphology, genetic characterization and molecular phylogeny of pinworm Skrjabinema longicaudatum sp. n. (Oxyurida: Oxyuridae) from the endangered Tibetan antelope Pantholops hodgsonii (Abel) (Artiodactyla: Bovidae)

2020 ◽  
Author(s):  
Yi-Fan Cao ◽  
Hui-Xia Chen ◽  
Yang Li ◽  
Dang-Wei Zhou ◽  
Shi-Long Chen ◽  
...  
Keyword(s):  
New Species ◽  
Ribosomal Dna ◽  
Dna Sequences ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Small Subunit ◽  
Sister Relationship ◽  
Tibetan Antelope ◽  
A New Species

Abstract Background: The Tibetan antelope Pantholops hodgsonii (Abel) (Artiodactyla: Bovidae) is an endangered species of mammal endemic to the Qinghai-Tibetan Plateau. Parasites and parasitic diseases are considered to be important threats in the conservation of the Tibetan antelope. However, our present knowledge of the composition of the parasites of the Tibetan antelope remains limited. Methods: Large numbers of nematode parasites were collected from a dead Tibetan antelope. The morphology of these nematode specimens was observed using light and scanning electron microscopy. The nuclear and mitochondrial DNA sequences, i.e. small subunit ribosomal DNA (18S), large subunit ribosomal DNA (28S), internal transcribed spacer (ITS) and cytochrome c oxidase subunit 1 (cox1), were amplified and sequenced for molecular identification. Moreover, phylogenetic analyses were performed using maximum likelihood (ML) inference based on 28S and 18S + 28S + cox1 sequence data, respectively, in order to clarify the systematic status of these nematodes.Results: Integrated morphological and genetic evidence reveals these nematode specimens to be a new species of pinworm Skrjabinema longicaudatum (Oxyurida: Oxyuridae). There was no intraspecific nucleotide variation between different individuals of S. longicaudatum n. sp. in the partial 18S, 28S, ITS and cox1 sequences. However, a high level of nucleotide divergence was revealed between the new species and its congeners in 28S (8.36%) and ITS (20.3–23.7%) regions, respectively. Molecular phylogenetic results suggest that the genus Skrjabinema should belong to the subfamily Oxyurinae (Oxyuroidea: Oxyuridae), instead of the subfamily Syphaciidae or Skrjabinemiinae in the traditional classification, as it formed a sister relationship to the genus Oxyuris. Conclusions: A new species of pinworm Skrjabinema longicaudatum n. sp. (Oxyurida: Oxyuridae) is described. Skrjabinema longicaudatum n. sp. represents the first species of Oxyurida (pinworm) and the fourth nematode species reported from the Tibetan antelope. Our results contribute to the knowledge of the species diversity of parasites from the Tibetan antelope, and clarify the systematic position of the genus Skrjabinema.

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