scholarly journals On the Non-Specific Stimulation of Agglutinins. With Especial Reference to the Enteric Fevers and Typhus Fever

1929 ◽  
Vol 28 (4) ◽  
pp. 418-448 ◽  
Author(s):  
A. Felix
Keyword(s):  
Qualitative Method ◽  
Published Data ◽  
Enteric Infection ◽  
Widal Test ◽  
Enteric Infections ◽  
The Difference ◽  
Specific Stimulation ◽  
Enteric Group ◽  
Typhus Fever ◽  
Stimulation Of

(1) The review of the published data furnishes additional evidence in support of the view that no technique whatsoever, Dreyer's technique included, based on the quantitative method of the agglutination reaction hitherto used, is capable of affording a differentiation between inoculation and infection agglutinins.(2) These techniques are concerned always exclusively in the demonstration of the labilotropic H agglutinins ofB. typhosusandB. paratyphosusA. and B. and it is the behaviour of these agglutinins that is the responsible factor in producing the phenomena.(3) In various febrile conditions in inoculated individuals these H agglutinins undergo a re-stimulation resulting in a curve of agglutination which is indistinguishable from that due to specific stimulation. The re-stimulation of the labilotropic inoculation agglutinins is of the same nonspecific character (i.e.heterologous) in the course of enteric infections as in the course of other febrile diseases.(4) The observation of this non-specific re-stimulation is independent of the technique used; living bacilli and suspensions preserved with phenol or formalin (Dreyer's technique included) do not in this respect behave differently.(5) The proposed qualitative method for the Widal test depends, in inoculated individuals, exclusively upon the behaviour of the stabilotropic O agglutinins. In their presence it is capable of affording the certain diagnosis of an enteric infection; in their absence the negative result of the test is not conclusive; if T.A.B. vaccine has been used it is only possible to diagnose enteric group without being able to differentiate typhoid from paratyphoid A. or B.; if T. vaccine has been used then A. or B. infection can be differentiated but not T.(1) The conclusions previously arrived at by means of the qualitative method of the Widal test were fully confirmed. By eliminating the labilotropic H agglutinins from any consideration—in the case of previously sensibilised individuals—agglutination due to the specific stimulation in active enteric infection can be distinguished definitely from that due to the nonspecific re-stimulation by various febrile diseases.(2) Normal and immune O agglutinins forB. typhosusandB. paratyphosusA. and B., as well as those forB. proteusX 19, are not liable to non-specific stimulation in the course of various febrile diseases.(3) One more of the supposed differences in nature between the Widal test and the Weil-Felix test is thereby eliminated.(4) The difference in the response to non-specific stimulation shown to exist in stabilotropic and labilotropic agglutination seems more likely to be one of degree than one in nature and needs further investigation.

scholarly journals Double enteric infection (‘la fièvre typhoïde intriquée’). An account of an epidemic

1951 ◽  
Vol 49 (2-3) ◽  
pp. 299-314 ◽  
Author(s):  
A. Batty Shaw ◽  
H. A. F. Mackay
Keyword(s):  
Water Supply ◽  
Polymyxin B ◽  
Morbidity Rate ◽  
Clinical Aspects ◽  
Enteric Infection ◽  
Therapeutic Effects ◽  
Cultural Methods ◽  
Enteric Infections ◽  
Enteric Group ◽  
Fièvre Typhoïde

1. A double enteric infection is defined as the simultaneous infection of an individual or group of individuals with two organisms of the enteric group. The literature of the previously recorded cases and epidemics, in which the diagnosis of double enteric infection has been established by cultural methods, is reviewed.2. An account is given of a double enteric outbreak of seventy–six cases which occurred amongst British troops and police at Acre, Palestine, in 1948. The infection is thought to have been due to contamination of the water supply with sewage during the civil disturbances. The diagnosis was established bacteriologi–cally in seventy-four cases (93.3%);Salm. typhi was isolated in forty–three cases, Salm. paratyphi B in three, and both Salm. typhi and Salm. paratyphi B from twenty–eight cases. There were three fatal cases in the epidemic (mortality rate = 3·94%).3. The morbidity rate from enteric fever among the infantry unit was 60%, and among the Palestine police, 17%. The possible reasons for this difference is discussed, and the conclusion drawn that it was probably due to the Palestine policemen being older men, with longer overseas service and more 'seasoned' to life in subtropical conditions.4. Thirteen cases were treated with polymyxin B (15 mg. 4-hourly for 4-day periods); with this small dose no beneficial therapeutic effects were observed, and ten cases showed evidence of renal damage while under treatment.5. The bacteriology, epidemiology and clinical aspects of double enteric infections are discussed. The diagnosis of a double enteric infection may be established with the greatest certainty by blood culture. Such infections are usually water-borne or milk-borne, and tend to occur when there has been a severe breach of hygiene, e.g. in the contamination of a water supply by sewage. The claim that the prognosis in instances of double enteric infection is worse than with single infections is not supported by the experiences at Acre where the three fatal cases occurred in cases infected with Salm. typhi alone.

scholarly journals Qualitative or Quantitative Methods in the Serological Diagnosis of Enteric Infections?

1932 ◽  
Vol 32 (4) ◽  
pp. 523-528 ◽  
Author(s):  
E. S. Horgan
Keyword(s):  
Qualitative Method ◽  
Quantitative Methods ◽  
Serological Diagnosis ◽  
Enteric Infection ◽  
Enteric Infections

1. “Normal” agglutinins for B. typhosus and B. paratyphosus B appear to be conclusively of the O type.2. Such agglutinins appear to be of consistently low titre and are absent in many sera.3. Normal agglutinins for B. paratyphosus A have not been encountered.4. O agglutinins are readily produced by inoculation of a T.A.B. vaccine, and may rise to as high titres as those encountered during an enteric infection.5. It is impossible to fix any limit for residual O agglutinins in inoculated persons.6. The value of examining for O agglutinins in enteric cases is confirmed.7. Some fallacies of a purely qualitative method are pointed out, and a plea is made for the inclusion of the principles of qualitative receptor analysis in a quantitative Widal.

Antigen-specific stimulation and trans-stimulation of T cells in long-term culture

1979 ◽  
Vol 9 (9) ◽  
pp. 665-670 ◽  
Author(s):  
Andrei A. Augustin ◽  
Michael H. Julius ◽  
Humberto Cosenza
Keyword(s):  
T Cells ◽  
Long Term Culture ◽  
Specific Stimulation ◽  
Stimulation Of

Determination of Resistance to Abrasive Wear. VIII. Influence of Softeners in Tests with the Akron-Croydon and Du Pont Machines

1949 ◽  
Vol 22 (1) ◽  
pp. 259-262
Author(s):  
J. F. Morley
Keyword(s):  
Unit Area ◽  
Published Data ◽  
The Road ◽  
Abrasive Surface ◽  
Abrasive Power ◽  
The Difference ◽  
On The Road ◽  
The Right ◽  
Abrasion Testing

Abstract These experiments indicate that softeners can influence abrasion resistance, as measured by laboratory machines, in some manner other than by altering the stress-strain properties of the rubber. One possible explanation is that the softener acts as a lubricant to the abrasive surface. Since this surface, in laboratory abrasion-testing machines, is relatively small, and comes repeatedly into contact with the rubber under test, it seems possible that it may become coated with a thin layer of softener that reduces its abrasive power. It would be interesting in this connection to try an abrasive machine in which a long continuous strip of abrasive material was used, no part of it being used more than once, so as to eliminate or minimize this lubricating effect. The fact that the effect of the softener is more pronounced on the du Pont than on the Akron-Croydon machine lends support to the lubrication hypothesis, because on the former machine the rate of wear per unit area of abrasive is much greater. Thus in the present tests the volume of rubber abraded per hr. per sq. cm. of abrasive surface ranges from 0.03 to 0.11 cc. on the du Pont machine and from 0.0035 to 0.0045 cc. on the Akron-Croydon machine. On the other hand, if the softener acts as a lubricant, it would be expected to reduce considerably the friction between the abrasive and the rubber and hence the energy used in dragging the rubber over the abrasive surface. The energy figures given in the right-hand columns of Tables 1 and 3, however, show that there is relatively little variation between the different rubbers. As a test of the lubrication hypothesis, it would be of interest to vary the conditions of test so that approximately the same amount of rubber per unit area of abrasive is abraded in a given time on both machines; this should show whether the phenomena observed under the present test conditions are due solely to the difference in rate of wear or to an inherent difference in the type of wear on the two machines. This could most conveniently be done by considerably reducing the load on the du Pont machine. In the original work on this machine the load was standardized at 8 pounds, but no figures are quoted to show how abrasion loss varies with the load. As an addition to the present investigation, it is proposed to examine the effect of this variation with special reference to rubbers containing various amounts and types of softener. Published data on the influence of softeners on the road wear of tire rubbers do not indicate anything like such large effects as are shown by the du Pont machine. This throws some doubt on the value of this machine for testing tire tread rubbers, a conclusion which is confirmed by information obtained from other workers.

Variation in Skin Cancer Pattern in the Southwestern Region of Pakistan

2021 ◽  
Vol 15 (12) ◽  
pp. 3402-3404
Author(s):  
Hina , Manzoor ◽  
Najeeb Ahmad ◽  
Zafar H Tanveer ◽  
Khush Naseed Ahmed ◽  
Munir , Ahmed ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Skin Cancer ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Published Data ◽  
Non Melanoma Skin Cancer ◽  
Broad Term ◽  
The Difference ◽  
Field Workers ◽  
Melanoma Skin

Background: Skin cancer is a broad term that refers to a variety of different types of cancer. It is usually recognized as non-melanoma and melanoma skin cancer. In many parts of the world, the prevalence is high, with significant ecological and ethical variation. Objectives: Objective was to determine demographic and histological features of skin cancer in Southwest region of Pakistan. Methodology: This retrospective study was carried out on skin cancer 1169 cases of Centre for Nuclear Medicine and Radiotherapy (CENAR) in Quetta. The data from January 2000 to December 2009 (10Years) was retrieved from record. The aim was to determine the importance of skin cancer in this area, its gender wise distribution and its pathological types. Results: Record of total 9308 cancer patients was retrieved from patients presenting to CENAR Quetta. From 9308 case, 1169(12.5%) patients were of skin cancer which was second most prevalent category of cancer in this area. Prevalence was higher in males with 713(61%) cases as compared to females. Pathologically with 634(54%) cases, the most prevalent category was Squamous cell carcinoma (SCC). Conclusion: Skin cancer is wide-spread type of cancer in patients of south-west region of Pakistan. The findings of this study are not aligned with published data. The difference is because of high altitude of the study area, dry climate and long skin exposure particularly in low socio-economic field workers. Keywords: Skin cancer, gender, Melanoma skin cancer (MSC), Squamous cell carcinoma (SCC), Non-melanoma skin cancer (NMSC), Basal cell carcinoma (BCC),

Enhanced coupling of adenylate cyclase to lipolysis in permeabilized adipocytes from trained rats

1991 ◽  
Vol 71 (1) ◽  
pp. 23-29 ◽  
Author(s):  
T. Izawa ◽  
T. Komabayashi ◽  
T. Mochizuki ◽  
K. Suda ◽  
M. Tsuboi
Keyword(s):  
Adenylate Cyclase ◽  
Dose Response ◽  
Response Curve ◽  
Positive Relationship ◽  
Regression Coefficient ◽  
Permeabilized Cells ◽  
Major Mechanism ◽  
The Difference ◽  
And Control ◽  
Stimulation Of

Digitonin-permeabilized adipocytes were used to study the coupling of adenylate cyclase (AC) to lipolysis in exercise-trained rats. Isoproterenol-(IPR) stimulated lipolysis in permeabilized cells was significantly greater in trained than in control rats. Under essentially identical conditions, the dose-response curve for IPR stimulation of AC activity in the absence of 3-isobutyl-1-methylxanthine was similar in trained and control rats. However, the potency of stimulation by IPR as a percentage of the basal level was greater in trained rats. AC activity and lipolysis in the presence of 3-isobutyl-1-methylxanthine were also significantly greater in trained than in control rats. Least-squares analysis by plotting the log AC vs. lipolysis values showed that the regression coefficient was about three-fold greater in trained than in control rats. The concentration of endogenous adenosine 3′,5′-cyclic monophosphate (cAMP) needed to produce a half-maximal lipolytic response was 18.58 and 10.81 pmol.min-1.10(6) cells-1 in control and trained rats, respectively. Thus a positive relationship existed between lipolysis and AC activity, with a tighter coupling in trained rats. Lipolysis in response to exogenous cAMP tended to be greater in trained than in control rats, and the difference was statistically significant for 50 microM and 10 mM cAMP. Our finding support the concept that the major mechanism of enhanced lipolysis in trained rats was an increase in the activity of enzymatic step(s) distal to cAMP.

Sign in / Sign up

Export Citation Format

Share Document