scholarly journals A whey complement fixation test. Its relation to whey agglutination and isolation of Brucella abortus from the milk of individual cows

1968 ◽  
Vol 66 (1) ◽  
pp. 19-26 ◽  
Author(s):  
I. D. Farrell ◽  
L. Robertson
Keyword(s):  
Brucella Abortus ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Ring Test ◽  
Eradication Programme ◽  
Preliminary Screening ◽  
Milk Samples ◽  
Positive Individual ◽  
The Individual ◽  
Milk Ring Test

SUMMARYTwo hundred and ninety-three milk ring test-positive individual milk samples were examined by whey complement fixation and agglutination tests and the results were compared with the isolation of B. abortus by cultures made from the milk.There was a closer correlation between whey CF tests and isolation of the organism than between whey AG tests and isolation. Brucella organisms were not isolated from 116 MRT-positive milk samples when the whey CF titre was < 1/10; whereas 9/104 (9%) isolations were made from milk when the whey AG titre was < 1/20.Because of its closer correlation with culture results it is suggested that the whey CF test would be valuable in an eradication programme for the detection of infected udders.The limitations of the milk ring test for the examination of individual milk samples are emphasized and it should be used only as a preliminary screening test. In the individual milk samples examined, 32% of the MRT-positives showed no evidence of infection by either the whey AG, whey CF, or culture tests.

Comparison of an indirect ELISA with the Brucella milk ring test for detection of antibodies to Brucella abortus in bulk milk samples

2001 ◽  
Vol 82 (1) ◽  
pp. 55-60 ◽  
Author(s):  
V.R. Vanzini ◽  
N.P. Aguirre ◽  
B.S. Valentini ◽  
S. Torioni de Echaide ◽  
C.I. Lugaresi ◽  
...  
Keyword(s):  
Brucella Abortus ◽  
Indirect Elisa ◽  
Ring Test ◽  
Milk Samples ◽  
Bulk Milk ◽  
Milk Ring Test

scholarly journals BACTERIOLOGICAL & SEROLOGICAL STUDY OF BRUCELLOSIS IN BUFFALO IN BAGHDAD

2006 ◽  
Vol 30 (2) ◽  
pp. 23-30
Author(s):  
S. A. Al-Bayatti
Keyword(s):  
Rose Bengal ◽  
Brucella Abortus ◽  
Agglutination Test ◽  
Ring Test ◽  
Serological Study ◽  
Isolation And Identification ◽  
Milk Samples ◽  
Milk Ring Test

To study the prevalence of brucellosis among buffalo in Baghdad, 144serum samples were collected and tested serologically using Rose Bengal PlateTest (RBPT) and Tube Agglutination Test (TAT), also 44 milk samples testedby Milk Ring Test (MRT). For isolation and identification of Brucella speciesand behavior, 15 buffalo aborted fetuses were used. And for evaluation of thehealth hazzard caused by the consumption of the locally manufactured creamfrom the milk of buffalo, 76 cream samples were used for cultivation.The results of the RBPT, TAT and MRT revealed the prevalence of thedisease in the percentages (38.19%, 29.86% and 20.45%) respectively.It was found that Brucella abortus was the main cause of abortion in thebuffalo (53.33%). There biotypes were the 8th and 3rd.The cream samples manufactured from the milk of buffalo doesn’t showhealth hazzard as a cause of brucellosis in human, because it didn’t revealed anyBrucella isolates in all samples used.

scholarly journals Brucella abortus RB51 and Hot Saline Extract from Brucella ovis as Antigens in a Complement Fixation Test Used To Detect Sheep Vaccinated with Brucella abortus RB51

2001 ◽  
Vol 8 (1) ◽  
pp. 119-122 ◽  
Author(s):  
Rosanna Adone ◽  
Franco Ciuchini
Keyword(s):  
Immune Responses ◽  
Brucella Abortus ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Infected Sheep ◽  
Fixation Test ◽  
Anticomplementary Activity ◽  
Serum Samples ◽  
Saline Extract ◽  
Brucella Ovis

ABSTRACT The efficacy of Brucella abortus RB51 and hot saline extract (HSE) from Brucella ovis as antigens in complement fixation (CF) tests was comparatively evaluated in detecting immune responses of sheep vaccinated with B. abortus strain RB51. For this study, four 5-month-old sheep were vaccinated subcutaneously with 5 × 109 CFU of RB51, and two sheep received saline. Serum samples collected at different times after vaccination were tested for the presence of antibodies to RB51 by a CF test with RB51 as antigen, previously deprived of anticomplementary activity, and with HSE antigen, which already used as the official antigen to detectB. ovis-infected sheep. The results showed that vaccinated sheep developed antibodies which reacted weakly against HSE antigen and these antibodies were detectable for 30 days after vaccination. However, antibodies to RB51 could be detected for a longer period after vaccination by using homologous RB51 antigen in CF tests. In fact, high titers were still present at 110 days postvaccination with RB51 antigen. Sera from sheep naturally infected with B. ovisalso reacted to RB51 but gave lower titers than those detected by HSE antigen. As expected, all sera from RB51-vaccinated sheep remained negative when tested with standard S-type Brucella standard antigens.

Seroprevalence and molecular diagnosis of sheep brucellosis in Dakahlia governorate, Egypt

2021 ◽  
Vol 1 (1) ◽  
pp. 34-39
Author(s):  
Mohamed El-Diasty ◽  
◽  
Rana El-Said ◽  
Adel Abdelkhalek ◽  
◽  
...  
Keyword(s):  
Molecular Diagnosis ◽  
Tissue Sample ◽  
Serological Test ◽  
Ring Test ◽  
Antigen Test ◽  
Isolation Rate ◽  
Serological Tests ◽  
Milk Samples ◽  
Animal Populations ◽  
Milk Ring Test

Brucellosis is an endemic disease among livestock and humans in Egypt. Sheep are the most common type of livestock ruminant in Egypt and considered the fundamental etiology for spreading and maintaining B. melitensis either in human being or animal populations. In the current study, we investigated the seroprevalence of brucellosis in sheep herds reared in Bilqase, one of the biggest cities at Dakahlia governorate in Egypt's Delta region. In total, 610 sheep from seven herds were investigated. Anti-Brucella antibodies were detected in 48 (7.8%) samples tested by Buffered Acidified Plate Antigen Test (BAPAT), in 44 (7.2%) samples tested by Rose Bengal Plate Test (RBPT) and in 41 (6.7%) samples tested by Milk Ring Test (MRT). The isolation rate was 29.6% (16 out of 54 examined samples). Brucella organism was isolated from three aborted fetuses, one tissue sample of slaughtered serologically positive ewe and 12 milk samples. The Abortus Melitensis Ovis Suis-PCR (AMOS-PCR) confirmed all Brucella strains as B. melitensis. More than three successive negative serological tests are required to declare that the infected herd is free from brucellosis. In conclusion, no single serological test could conclusively diagnose brucellosis in endemic areas. Confirmation of results with molecular diagnosis or culture is indispensable in diagnosis. B. melitensis was the prevalent serotype among sheep in Dakahlia governorate

scholarly journals Protective Effect of the Nramp1 BB Genotype against Brucella abortus in the Water Buffalo (Bubalus bubalis)

2006 ◽  
Vol 75 (2) ◽  
pp. 988-996 ◽  
Author(s):  
Rosanna Capparelli ◽  
Flora Alfano ◽  
Maria Grazia Amoroso ◽  
Giorgia Borriello ◽  
Domenico Fenizia ◽  
...  
Keyword(s):  
Brucella Abortus ◽  
Untranslated Region ◽  
Complement Fixation Test ◽  
Water Buffalo ◽  
Complement Fixation ◽  
Bubalus Bubalis ◽  
Lower Number ◽  
Agglutination Test ◽  
Intracellular Bacteria ◽  
Antibacterial Protein

ABSTRACT We tested 413 water buffalo cows (142 cases and 271 controls) for the presence of anti-Brucella abortus antibodies (by the skin test, the agglutination test, and the complement fixation test) and the Nramp1 genotype (by capillary electrophoresis). Four alleles (Nramp1A, -B, -C, and -D) were detected in the 3′ untranslated region of the Nramp1 gene. The BB genotype was represented among only controls, providing evidence that this genotype confers resistance to Brucella abortus. The monocytes from the BB (resistant) subjects displayed a higher basal level of Nramp1 mRNA and a lower number of viable intracellular bacteria than did the monocytes from AA (susceptible) subjects. The higher basal level of the antibacterial protein Nramp1 most probably provides the BB animals with the possibility of controlling bacteria immediately after their entry inside the cell.

scholarly journals Comparison of radioimmunoassay with the complement fixation test and the indirect haemolysis test in the field diagnosis of bovine brucellosis

1983 ◽  
Vol 90 (1) ◽  
pp. 67-70 ◽  
Author(s):  
R. J. Chappel ◽  
J. Hayes
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
Supporting Evidence ◽  
Bovine Brucellosis ◽  
Eradication Programme ◽  
Female Cattle ◽  
The Rose ◽  
Supplementary Test ◽  
Haemolysis Test

SUMMARYSera were collected from female cattle in 118 commercial herds being subjected to a programme to eradicate brucellosis by test and slaughter, in an area in which vaccination of heifer calves with Brucella abortus strain 19 was compulsory. Of 4583 sera positive by the Rose Bengal plate test, the brucellosis radioimmunoassay was positive for 1524, the complement fixation test for 1363 and the indirect haemolysis test for 1141. These figures, and supporting evidence from the eradication programme, suggest that the radioimmunoassay may be a useful supplementary test in problem herds.

scholarly journals Serological, cultural, and molecular evidence of Brucella infection in small ruminants in Pakistan

2015 ◽  
Vol 9 (05) ◽  
pp. 470-475 ◽  
Author(s):  
Shahzad Ali ◽  
Shamim Akhter ◽  
Heinrich Neubauer ◽  
Falk Melzer ◽  
Iahtasham Khan ◽  
...  
Keyword(s):  
Causative Agent ◽  
Brucella Abortus ◽  
Small Ruminants ◽  
Ring Test ◽  
Effective Control ◽  
Molecular Evidence ◽  
Serum Samples ◽  
Qrt Pcr ◽  
Milk Samples ◽  
Eradication Strategy

Introduction: The objectives of the present study were to determine the seroprevalence and identify the causative agent of brucellosis in small ruminants in Pakistan. Methodology: A total of 278 serum and 212 milk samples were collected from sheep and goats that had close contact with seropositive bovine herds. Data related to age, sex, location, and breed were collected on the sampling day. Serum and milk samples were initially screened using two different Rose Bengal plate test (RBPT) antigens and a milk ring test (MRT). Seropositive samples were subjected to bacterial isolation and PCR analysis using Brucella genus-specific (bcsp31) and Brucella species-specific (IS711 for Brucella abortus and Brucella melitensis) quantitative real-time polymerase chain reactions (qRT-PCR). Results: Twenty-four (8.6%) serum samples were positive by RBPT. Twenty (9.4%) animals were positive for Brucella antibodies using MRT. No Brucella isolates were obtained from the examined blood and milk samples. Of the 24 seropositive serum samples, 18 (75%) were positive in the Brucella genus-specific (bcsp31) and Brucella abortus-specific (IS711) qRT-PCR, respectively. Conclusions: Brucella abortus was identified as causative agent of ovine and caprine brucellosis in Pakistan. Results of this study can be used for the development of an effective control and eradication strategy for brucellosis in livestock, especially small ruminants.

THE USE OF PRESERVED MILK SAMPLES IN THE BRUCELLA MILK RING TEST

1977 ◽  
Vol 53 (4) ◽  
pp. 184-186 ◽  
Author(s):  
I. G. BLYTHMAN ◽  
A. J. FORMAN
Keyword(s):  
Ring Test ◽  
Milk Samples ◽  
Milk Ring Test

scholarly journals Complement Fixation Test To Assess Humoral Immunity in Cattle and Sheep Vaccinated with Brucella abortusRB51

1999 ◽  
Vol 6 (6) ◽  
pp. 787-790 ◽  
Author(s):  
Rosanna Adone ◽  
Franco Ciuchini
Keyword(s):  
Humoral Immunity ◽  
Brucella Abortus ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Deficient Mutant ◽  
Anticomplementary Activity ◽  
Bovine Brucellosis ◽  
Serologic Tests ◽  
Rough Strain ◽  
Cattle And Sheep

ABSTRACT The live attenuated Brucella abortus strain RB51 is a rifampin-resistant, lipopolysaccharide (LPS) O-chain-deficient mutant of virulent B. abortus 2308. The reduced O-chain content in RB51 prevents this bacterium from inducing antibodies detectable by the conventional serologic tests for bovine brucellosis diagnosis that mainly identify antibodies to LPS. The absence of available serologic tests for RB51 also complicates the diagnosis of possible RB51 infections in humans exposed to this strain. The purpose of this study was to evaluate the suitability of a complement fixation (CF) test performed with the rough strain B. abortus RB51, previously deprived of anticomplementary activity, in detecting anti-B. abortus RB51 antibodies in cattle and sheep experimentally vaccinated with this strain. The results of this study showed that a CF test with RB51 as the antigen is able to specifically detect antibodies following RB51 vaccination in cattle and sheep. In addition, this method could be a useful tool for detecting B. abortus RB51 infection in humans.

scholarly journals A serological prevalence survey of Brucella abortus in cattle of rural communities in the province of KwaZulu-Natal, South Africa : article

2008 ◽  
Vol 79 (1) ◽  
Author(s):  
U.W. Hesterberg ◽  
R. Bagnall ◽  
K. Perrett ◽  
B. Bosch ◽  
R. Horner ◽  
...  
Keyword(s):  
Rural Communities ◽  
Brucella Abortus ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Primary Sampling Unit ◽  
Prevalence Survey ◽  
Test Sensitivity ◽  
Apparent Prevalence ◽  
Kwazulu Natal ◽  
The Rose

A serological survey of Brucella abortus in cattle originating from communal grazing areas of Kwa Zulu Natal was carried out between March 2001 and December 2003. The survey was designed as a 2-stage survey, considering the diptank as the primary sampling unit. In total 46 025 animals from 446 diptanks of 33 magisterial districts were sampled and tested using the Rose Bengal test and Complement Fixation Test. The apparent prevalence at district level was adjusted for clustering, diagnostic test sensitivity and specificity, and mapped using ArcView version 3.3. The prevalence of brucellosis in communal grazing areas of Kwa-Zulu Natal was found to be 1.45 % (0.84-2.21 %) and varied from 0 to 15.6% between magisterial districts. In 19 of the 33 magisterial districts no serological reactors were observed. A large variation in prevalence was found within diptank areas. Brucellosis was found to be most prevalent in the northeastern area of the province. The findings of the survey are discussed.

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