Involvement of a Fishing Community in the Eradication of the Introduced Cactus Mouse (Peromyscus eremicus cedrosensis) from San Benito Oeste Island, Mexico

San Benito Archipelago is internationally important for the conservation of 13 species of seabirds. San Benito Oeste, the largest and only inhabited island, was declared mammal-free in 2000 after a series of eradications conducted in collaboration between the fishing cooperative Pescadores Nacionales de Abulón, the Mexican conservation organization, Grupo de Ecología y Conservación de Islas, A.C., and the Mexican Government. The archipelago remained mammal-free until 2006, when an unusual invader, the Cedros island cactus mouse (Peromyscus eremicus cedrosensis), was accidentally introduced to San Benito Oeste island. The same collaboration scheme involving locals, conservationists, and authorities was once again put in motion, delivering tangible results. Research informed the mouse eradication strategy, the local community supported the operation, and the mouse eradication was successfully implemented in December 2013. To date (8 years later), no mammals have been recorded in the archipelago, which suggests community-led island biosecurity is working. In addition, this collaborative restoration work contributed to the creation of the Baja California Pacific Islands Biosphere Reserve, protecting 21 islands, including the San Benito Archipelago, and 97 islets in the Mexican Pacific.

Effects of Artificial Aging on Seed Vigor and Physiological Characteristics of the Invasive Alien Plant Aegilops tauschii

The control of invasive plants depends to some extent on the persistence of the soil seed bank. The high temperature (40 °C) accompanied with high humidity (95%) method was used to treat the seeds of the invasive species Aegilops tauschii Coss. The aim of our study was to evaluate the seed vigor and longevity by accelerating aging, to serve as a reference for the evaluation of invasion potential of A. tauschii and corresponding eradication strategy adoption. The results showed that with the extension of aging time, the germination rate (GR), energy (GE), and index (GI) of A. tauschii seeds reduced. All the results were significantly different from the control (CK) since the second day ( p < 0.05). During the aging process, the seed relative water content (RWC), relative electric conductivity (REC), and thiobarbituric acid (TBARS) level increased, while the superoxide dismutase (SOD) gradually decreased after second day of seed aging. In addition, the aging treatment also caused a continuous decrease in the endogenous gibberellin (GA 3 ) content and a continuous increase in the abscisic acid (ABA) content of the seeds. Thus, a sharp decrease in the GA 3 /ABA ratio was evident. Finally, the study revealed that the germination inhibitors of A. tauschii were mainly concentrated in the glumes, which was revealed during seed aging. The results of the comprehensive analysis indicated that the changes of the above-mentioned internal factors eventually led to a rapid decline of the seed vigor of A. tauschii . Based on the results of the aging test, and the distribution characteristics of A. tauschii seeds in the soil seed banks, we recommend that soil solarization is one of the effective methods to eradicate the seed bank of A. tauschii .

Environmental Surveillance for Risk Assessment in the Context of a Phase 2 Clinical Trial of Type 2 Novel Oral Polio Vaccine in Panama

Environmental surveillance was recommended for risk mitigation in a novel oral polio vaccine-2 (nOPV2) clinical trial (M5-ABMG) to monitor excretion, potential circulation, and loss of attenuation of the two nOPV2 candidates. The nOPV2 candidates were developed to address the risk of poliovirus (PV) type 2 circulating vaccine-derived poliovirus (cVDPV) as part of the global eradication strategy. Between November 2018 and January 2020, an environmental surveillance study for the clinical trial was conducted in parallel to the M5-ABMG clinical trial at five locations in Panama. The collection sites were located upstream from local treatment plant inlets, to capture the excreta from trial participants and their community. Laboratory analyses of 49 environmental samples were conducted using the two-phase separation method. Novel OPV2 strains were not detected in sewage samples collected during the study period. However, six samples were positive for Sabin-like type 3 PV, two samples were positive for Sabin-like type 1 PV, and non-polio enteroviruses NPEVs were detected in 27 samples. One of the nOPV2 candidates has been granted Emergency Use Listing by the World Health Organization and initial use started in March 2021. This environmental surveillance study provided valuable risk mitigation information to support the Emergency Use Listing application.

Correction to: Climate-proofing a malaria eradication strategy

An amendment to this paper has been published and can be accessed via the original article.

Climate-proofing a malaria eradication strategy

Two recent initiatives, the World Health Organization (WHO) Strategic Advisory Group on Malaria Eradication and the Lancet Commission on Malaria Eradication, have assessed the feasibility of achieving global malaria eradication and proposed strategies to achieve it. Both reports rely on a climate-driven model of malaria transmission to conclude that long-term trends in climate will assist eradication efforts overall and, consequently, neither prioritize strategies to manage the effects of climate variability and change on malaria programming. This review discusses the pathways via which climate affects malaria and reviews the suitability of climate-driven models of malaria transmission to inform long-term strategies such as an eradication programme. Climate can influence malaria directly, through transmission dynamics, or indirectly, through myriad pathways including the many socioeconomic factors that underpin malaria risk. These indirect effects are largely unpredictable and so are not included in climate-driven disease models. Such models have been effective at predicting transmission from weeks to months ahead. However, due to several well-documented limitations, climate projections cannot accurately predict the medium- or long-term effects of climate change on malaria, especially on local scales. Long-term climate trends are shifting disease patterns, but climate shocks (extreme weather and climate events) and variability from sub-seasonal to decadal timeframes have a much greater influence than trends and are also more easily integrated into control programmes. In light of these conclusions, a pragmatic approach is proposed to assessing and managing the effects of climate variability and change on long-term malaria risk and on programmes to control, eliminate and ultimately eradicate the disease. A range of practical measures are proposed to climate-proof a malaria eradication strategy, which can be implemented today and will ensure that climate variability and change do not derail progress towards eradication.

A Simplified SARS-CoV-2 Pseudovirus Neutralization Assay

COVID-19 is an ongoing pandemic caused by the highly infectious coronavirus SARS-CoV-2 that is engaging worldwide scientific research to find a timely and effective eradication strategy. Great efforts have been put into anti-COVID-19 vaccine generation in an effort to protect the world population and block SARS-CoV-2 spread. To validate the protective efficacy of the vaccination campaign and effectively control the pandemic, it is necessary to quantify the induction of neutralizing antibodies by vaccination, as they have been established to be a correlate of protection. In this work, a SARS-CoV-2 pseudovirus neutralization assay, based on a replication-incompetent lentivirus expressing an adapted form of CoV-2 S protein and an ACE2/TMPRSS2 stably expressing cell line, has been minimized in terms of protocol steps without loss of accuracy. The goal of the present simplified neutralization system is to improve SARS-CoV-2 vaccination campaign by means of an easy and accessible approach to be performed in any medical laboratory, maintaining the sensitivity and quantitative reliability of classical serum neutralization assays. Further, this assay can be easily adapted to different coronavirus variants by simply modifying the pseudotyping vector.

Vaccination as a Control Tool in Bovine Tuberculosis: Social Media Monitoring to Assess Public Response to Government Policy Development and Implementation

Vaccine hesitancy does not only concern human vaccines but incorporates One Health policies also; including vaccination of cattle and badgers as part of the government’s bovine tuberculosis eradication strategy for England. Both digital and social media can propagate healthcare misinformation and thus affect vaccine policy support. The use of social media monitoring to understand real-time public perceptions of One Health policies is crucial to identify misinformation and address public concerns appropriately to achieve successful policy implementation. Digital and social media data surrounding two government announcements regarding the bovine tuberculosis eradication strategy for England were collected and screened using the Meltwater media monitoring platform. Communication patterns were studied using InfraNodus. Twitter analysis was conducted to identify key influencers, public engagement, and trending communications. Online social media activity increased rapidly after each announcement. Initially, badger culling took primary public concern and major influencers were identified. Cattle vaccination dominated discussion after the second announcement, with public perception being influenced by increased online activity from news sites, animal welfare charities, governmental bodies, and medical professionals. The greatest ambiguity towards the strategy was detected within farming communities, with the main disparity existing between cattle vaccination and badger culling opinions. Social media monitoring has potential use in surveying public perception of government policy, both prior to, and after implementation to identify and address areas of miscommunication and misinformation to improve public support for One Health policies.