Genetic population structure of Neanthes succinea (Polychaeta: Nereididae)

1992 ◽  
Vol 72 (3) ◽  
pp. 511-517 ◽  
Author(s):  
M. Abbiati ◽  
F. Maltagliati
Keyword(s):  
Population Structure ◽  
Mediterranean Sea ◽  
Genetic Identity ◽  
Isolated Populations ◽  
Genetic Population ◽  
Discriminant Locus ◽  
Two Samples ◽  
The Mean ◽  
Neanthes Succinea ◽  
F Statistics

Two samples of Neanthes succinea (Frey & Leuckart 1847) from the Mediterranean Sea were investigated. Twenty-one loci were analysed; seven of them were polymorphic in both populations. The level of heterozygosity was 2.2% and 4.4% in populations from the Tyrrhenian and Adriatic coasts respectively. The value of Nei's genetic identity index (1=0.965), together with the mean F(IT) (=0.350), shows that the samples can be considered reproductively isolated populations. F-statistics indicate that ME-1 is the discriminant locus between populations.

The genetic population structure of Thunnus thynnus (Linnaeus, 1758) in the Mediterranean Sea, a controversial issue

2019 ◽  
Vol 35 (2) ◽  
pp. 436-443
Author(s):  
Silvia Livi ◽  
Teresa Romeo ◽  
Sabina De Innocentiis ◽  
Claudia Greco ◽  
Pietro Battaglia ◽  
...  
Keyword(s):  
Population Structure ◽  
Mediterranean Sea ◽  
Controversial Issue ◽  
Genetic Population Structure ◽  
Thunnus Thynnus ◽  
Genetic Population ◽  
The Mediterranean

scholarly journals Rapid development of 56 novel microsatellite markers for the benthic freshwater bug Aphelocheirus aestivalis using Illumina paired-end sequencing data and M13-tailed primers

2020 ◽  
Vol 47 (12) ◽  
pp. 9995-10003
Author(s):  
Agnieszka Kaczmarczyk-Ziemba
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Microsatellite Markers ◽  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Rapid Development ◽  
Whole Genome ◽  
Sequencing Data ◽  
Isolated Populations ◽  
Two Samples

AbstractThe freshwater true bug Aphelocheirus aestivalis (Aphelocheiridae) is widely distributed in Europe but occurs rather locally and often in isolated populations. Moreover, it is threatened with extinction in parts of its range. Unfortunately, little is known about the genetic diversity and population structure due to the lack of molecular tools for this species. Thus, to overcome the limitations, a whole-genome sequencing has been performed to identify polymorphic microsatellite markers for A. aestivalis. The whole-genome sequencing has been performed with the Illumina MiSeq platform. Obtained paired-end reads were processed and overlapped into 2,378,426 sequences, and the subset of 267 sequences containing microsatellite motifs were then used for in silico primer designing. Finally, 56 microsatellite markers were determined and 34 of them were polymorphic. Analyses performed in two samples (collected from Drawa and Gowienica rivers, respectively) showed that the number of alleles per locus ranged from 2 to 21, and the observed and expected heterozygosity varied from 0 to 0.933 and 0.064 to 0.931, respectively. The microsatellite markers developed in the present study provide new suitable tools available for the scientific community to study A. aestivalis population dynamics. The assessment of its genetic diversity and population structure will provide important data, that can be used in population management and conservation efforts, elucidating the broad- and fine-scale population genetic structure of A. aestivalis.

scholarly journals Genetic population structure of European anchovy in the Mediterranean Sea and the Northeast Atlantic Ocean using sequence analysis of the mitochondrial DNA control region

2013 ◽  
Vol 71 (2) ◽  
pp. 391-397 ◽  
Author(s):  
Jordi Viñas ◽  
Núria Sanz ◽  
Luis Peñarrubia ◽  
Rosa-Maria Araguas ◽  
José-Luis García-Marín ◽  
...  
Keyword(s):  
Population Structure ◽  
Atlantic Ocean ◽  
Mediterranean Sea ◽  
Control Region ◽  
Genetic Population ◽  
Mitochondrial Dna Control Region ◽  
European Anchovy ◽  
Northeast Atlantic ◽  
Northeast Atlantic Ocean ◽  
The Mediterranean

Abstract Viñas, J., Sanz, N., Peñarrubia, L., Araguas, R-M., García-Marín, J-L., Roldán, M-I., and Pla, C. 2014. Genetic population structure of European anchovy in the Mediterranean Sea and the Northeast Atlantic Ocean using sequence analysis of the mitochondrial DNA control region. – ICES Journal of Marine Science, 71: 391–397. The European anchovy (Engraulis encrasicolus) exhibits a complex population structure in the Mediterranean Sea and Northeast Atlantic Ocean. To resolve the population genetic structure of this species, we surveyed sequence variability in the mitochondrial (mtDNA) control region in samples (n = 563) from 13 locations in the Northeast Atlantic, the Bay of Biscay, and the Mediterranean Sea. Based on pairwise ΦSTs, SAMOVA, and multidimensional scaling, a complicated population structure composed of multiple populations emerged. Combining these results with those from previous population studies based on mitochondrial and nuclear markers, we identified nine genetically differentiated European anchovy populations: (i) Canary Islands; (ii) Cádiz; (iii) Alborán Sea; (iv) Garona; (v) Arcachon and Donostia; (vi) a large population in the northwestern Mediterranean, including Cadaqués, Gulf of Lyon, Elba, and Sicily; (vii) southern Adriatic; (viii) northern Adriatic; and (ix) Aegean Sea. We suggest that independent management strategies should be implemented for each genetically differentiated population, and, in cases where several fisheries stocks are recognized within an area of genetic homogeneity, each stock should be managed separately.

scholarly journals Genetic structure of natterjack toad (Epidalea calamita) populations in Flanders, Belgium, and its implications for conservation

2019 ◽  
Vol 40 (2) ◽  
pp. 193-205
Author(s):  
Joke Maes ◽  
Arend Raoul Van Oosten ◽  
Natalie Van Houtte ◽  
Erik Matthysen
Keyword(s):  
Population Structure ◽  
Genetic Structure ◽  
Habitat Management ◽  
Natural Populations ◽  
Conservation Priorities ◽  
Evolutionary Potential ◽  
Isolated Populations ◽  
Genetic Population ◽  
Wide Range ◽  
Epidalea Calamita

Abstract Unique evolutionary potential could be lost when a population goes extinct or when individuals are translocated to other existing populations. Therefore, in order to identify priorities and to predict the efficiency and consequences of conservation actions, information is needed on the genetic structure of natural populations. In the urbanized and diverse landscapes of Flanders, Belgium, natterjack toad (Epidalea calamita) populations have been declining over the last decades. Therefore, this species is subjected to a wide range of different types of conservation measures (e.g. habitat management, corridor development, translocations). However, more information is needed on its genetic population structure. In this study, we sampled egg clutches from six populations and studied their genetic structure with six microsatellite markers. In total, 184 samples from 99 different egg strings were genotyped. Observed heterozygosity was generally high, even for the small and isolated populations (overall mean HO = 0.43). The weak clustering by the Bayesian analyses (STRUCTURE, Adegenet and BAPS) does not allow us to make strong conclusions on the population structure. However, the significant ΦST values between the populations underline the importance of genetic information when conservation priorities are discussed. Unique evolutionary potential could be lost when one or more natterjack toad populations would go extinct, and translocation of individuals to other existing populations should be considered with caution.

Pilot study for cystic fibrosis neonatal screening: the Cuban experience

2020 ◽  
Vol 58 (11) ◽  
pp. 1857-1864
Author(s):  
Elisa M. Castells ◽  
Aramis Sánchez ◽  
Amarilys Frómeta ◽  
Yanin Mokdse ◽  
Nelson Ozunas ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Screening Program ◽  
Dried Blood Spots ◽  
Enzyme Linked Immunosorbent Assay ◽  
Perinatal Factors ◽  
Screening Process ◽  
Unequal Variance ◽  
National Network ◽  
Two Samples ◽  
The Mean

AbstractBackgroundIn Cuba, no screening program for cystic fibrosis (CF) has been implemented yet. The ultramicro enzyme-linked immunosorbent assay (UMELISA)® TIR NEONATAL has been developed for the measurement of immunoreactive trypsin (IRT) in dried blood spots on filter paper. The analytical performance of the kit was evaluated in the national network of laboratories.MethodsNewborn dried blood samples (DBS) were evaluated in 16 laboratories. An IRT/IRT/DNA protocol was followed using a cut-off value of 50 ng/mL. The mean, median and percentiles of the distribution were calculated and a two-sample t-test with unequal variance was used for statistical analysis. Influence of perinatal factors on IRT levels was analyzed.ResultsFrom January to June 2018, 6470 newborns were studied, obtaining a mean IRT value of 12.09 ng/mL (ranging 0–358 ng/mL) and a median of 8.99 ng/mL. Fifty-two samples (0.78%) were above the cut-off level and 16 samples (0.24%) were elevated in the re-screening process. One of them was confirmed positive by molecular biology (phe508del/c.3120 + 1G > A), constituting the first newborn screened and diagnosed early in Cuba. Second DBS samples were collected on average at 14 days and processed in the laboratory at 16 days of birth. Significant differences were observed (p < 0.05) when evaluating the influence of gender, birth weight (BW) and gestational age (GA) on the IRT values. Lower IRT concentrations were found in samples processed after 10 days of collection.ConclusionsThe performance of UMELISA® TIR NEONATAL in the laboratories has been satisfactory; hence CF newborn screening (NBS) was extended throughout the country from January 2019.

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