scholarly journals The lysidyl aminoacyl transfer RNA synthetase intron, a new marker for demosponge phylogeographics – case study on Neopetrosia

2015 ◽  
Vol 96 (2) ◽  
pp. 333-339
Author(s):  
Edwin Setiawan ◽  
Nicole J. De Voogd ◽  
John N.A. Hooper ◽  
Gert Wörheide ◽  
Dirk Erpenbeck
Keyword(s):  
Population Studies ◽  
Transfer Rna ◽  
Dispersal Patterns ◽  
Nuclear Introns ◽  
Potential Marker ◽  
Potential Alternative ◽  
Beta Subunit Gene ◽  
Aminoacyl Transfer ◽  
Intraspecific Relationships

Suitable genetic markers for population studies in sponges are necessary to further our understanding of biodiversity and dispersal patterns, and contribute to conservation efforts. Due to the slow mitochondrial substitution rates in demosponges, nuclear introns are among the preferable markers for phylogeographic studies, but so far only the second intron of the ATP synthetase beta subunit-gene (ATPSβ) has been successfully established. In the present study, we analyse the intron of the Lysidyl Aminoacyl Transfer RNA Synthetase (LTRS), another potential marker to study demosponge intraspecific relationships, on samples of Neopetrosia chaliniformis from various locations in the Indo-Pacific and compare its variation with a mitochondrial marker (CO2). LTRS recovers several reciprocal monophyletic groups among the Indo-Pacific N. chaliniformis and provides a potential alternative to ATPSβ.

scholarly journals Floral nectar chitinase is a potential marker for monofloral honey botanical origin authentication: A case study from loquat (Eriobotrya japonica Lindl.)

2019 ◽  
Vol 282 ◽  
pp. 76-83 ◽  
Author(s):  
Yue-Qin Song ◽  
Richard I. Milne ◽  
Hong-Xia Zhou ◽  
Xue-Long Ma ◽  
Jiang-Yu Fang ◽  
...  
Keyword(s):  
Eriobotrya Japonica ◽  
Botanical Origin ◽  
Floral Nectar ◽  
Potential Marker

scholarly journals The effect of hypermethylation on the functional properties of transfer ribonucleic acid. Formation of aminoacyl-transfer-ribonucleic acid

1969 ◽  
Vol 114 (2) ◽  
pp. 429-435 ◽  
Author(s):  
David J. Pillinger ◽  
John Hay ◽  
Ernest Borek
Keyword(s):  
Amino Acids ◽  
Ribonucleic Acid ◽  
Control Sample ◽  
Transfer Rna ◽  
E Coli ◽  
Functional Sites ◽  
The Individual ◽  
Almost All ◽  
Aminoacyl Transfer ◽  
Kinetics Of

1. The ability of chemically hypermethylated Escherichia coli B transfer RNA to accept 19 amino acids was studied and the results were compared with those obtained with a control sample of E. coli B transfer RNA incubated under similar conditions in the absence of methylating agent. 2. There is a marked decrease in the ability of the modified transfer RNA to accept amino acids in almost all instances. 3. The acceptance of cysteine appears to be unique in that it is enhanced in the hypermethylated transfer RNA. 4. More detailed studies on the kinetics of acceptance for six amino acids is presented, emphasizing the variation in response of the individual amino acids. 5. Increasing hypermethylation causes a progressive decrease in the amino acid acceptance. 6. The results are discussed in terms of methylation at functional sites within the transfer RNA and possible conformational alterations to the structure of the macromolecule.

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