The influence of the H-2 complex on responses to infection by Schistosoma mansoni in mice

Parasitology ◽  
1983 ◽  
Vol 86 (1) ◽  
pp. 19-30 ◽  
Author(s):  
Janet T. Jones ◽  
D. M. Mccaffery ◽  
J. R. Kusel
Keyword(s):  
Major Histocompatibility Complex ◽  
Antibody Response ◽  
Schistosoma Mansoni ◽  
Genetic Background ◽  
Worm Burden ◽  
Major Effect ◽  
Schistosomiasis Mansoni ◽  
Histocompatibility Complex ◽  
Faecal Egg Count ◽  
Antibody Titres

SUMMARYIn order to determine whether a given H-2 haplotype has similar effects on responses to schistosomiasis mansoni on different genetic backgrounds, mice of 2 pairs of congenic strains (H-2b and H-2k on BALB/c and C57BL/10 backgrounds) were infected. Worm burdens, mortality, splenomegaly, tissue and faecal egg counts, and antibody titres to worm and egg antigens were measured. The genetic background had a major effect on the genesis of splenomegaly, on the deposition of eggs in the spleen, the maximum faecal egg count, the antibody titre to egg and worm antigens and the rate of generation of antibody response. The H-2 haplotype was shown to consistently influence the maximum faecal egg count and the antibody titres. Worm burden was not influenced by genetic differences between strains and mortality differences were not significant. The data presented here indicate that the effect of the major histocompatibility complex on responses to infection is greatly influenced by the genetic background on which it is expressed.

scholarly journals Major histocompatibility complex recombinant R13 antibody response against bovine red blood cells

2020 ◽  
Vol 99 (10) ◽  
pp. 4804-4808
Author(s):  
N.G. Wilkinson ◽  
R.T. Kopulos ◽  
L.M. Yates ◽  
W.E. Briles ◽  
R.L. Taylor
Keyword(s):  
Major Histocompatibility Complex ◽  
Antibody Response ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Major Histocompatibility ◽  
Histocompatibility Complex

scholarly journals Egg excretion in the initial phase of experimental murine schistosomiasis mansoni: stability and association with worm burden

1995 ◽  
Vol 37 (4) ◽  
pp. 325-329 ◽  
Author(s):  
Regina Lunardi Rocha ◽  
Manoel Otávio da Costa Rocha ◽  
Ênio Roberto Pietra Pedroso ◽  
Enrico Antônio Colosimo ◽  
Paulo Marcos Zech Coelho
Keyword(s):  
Schistosoma Mansoni ◽  
Initial Phase ◽  
Worm Burden ◽  
Chronic Phase ◽  
Infection Intensity ◽  
Schistosome Infection ◽  
Schistosomiasis Mansoni

Stability of faecal egg excretion and correlation with results related to worm burden at the initial phase of schistosomiasis mansoni were observed in two groups of mice infected with different Schistosoma mansoni cercarial burdens, by means of analysis of quantitative parasitological studies and schistosome counts after perfusion. Thus, it may be stated that few quantitative parasitological stool examinations could be sufficient to express the infection intensity at the initial phase, on the same grounds that it was already demonstrated at the chronic phase. Furthermore, it is confirmed that the use of the number of eggs passed in the faeces as a tool to estimate the worm burden at the initial phase of schistosome infection is adequate.

Restoration of the antibody response to sheep erythrocytes in thymectomized Xenopus implanted with MHC-compatible or MHC-incompatible thymus

Development ◽  
1984 ◽  
Vol 84 (1) ◽  
pp. 287-302
Author(s):  
A. J. H. Gearing ◽  
Frances A. Cribbin ◽  
J. D. Horton
Keyword(s):  
Major Histocompatibility Complex ◽  
Antibody Response ◽  
Antibody Production ◽  
Blood Cells ◽  
Model System ◽  
T Helper Cell ◽  
T Helper ◽  
Histocompatibility Complex ◽  
Adult Thymus

These experiments make use of an amphibian model system for investigating the role of the thymus in T helper cell education. Clawed toads (Xenopus laevis), thymectomized at 7 days, are unable to mount an antibody response to thymus-dependent antigens, such as sheep red blood cells (SRBC). When thymectomized larvae are implanted with larval thymuses (either irradiated or non-irradiated), incompatible at the major histocompatibility complex (MHC) or with MHC-compatible or -incompatible ‘adult’ thymuses, their splenic plaque-forming cell response and serum haemolytic antibody production to SRBC are both restored, to some extent. However, levels of mercaptoethanol-resistant antibody were extremely poor in those animals implanted with MHC-incompatible ‘adult’ thymus. Larval thymus implants were shown, by ploidy-labelling studies, to become repopulated with host-derived lymphocytes. Whether or not these lymphocytes acquire their MHC restriction specificities in the thymus awaits clarification.

scholarly journals Immune response genes controlling responsiveness to major transplantation antigens. Specific major histocompatibility complex-linked defect for antibody responses to class I alloantigens.

1982 ◽  
Vol 155 (1) ◽  
pp. 303-320 ◽  
Author(s):  
G W Butcher ◽  
J R Corvalán ◽  
D R Licence ◽  
J C Howard
Keyword(s):  
Major Histocompatibility Complex ◽  
Antibody Response ◽  
Antibody Responses ◽  
Class I ◽  
Control Analysis ◽  
Major Histocompatibility ◽  
Histocompatibility Complex ◽  
Transplantation Antigens ◽  
Ir Genes

We have identified two major histocompatibility complex (MHC)-linked Ir genes that control the antibody response made by rats against class I major alloantigens. We have named these genes Ir-RT1Aa and Ir-RT1Ac. These Ir genes determine responsiveness of the immunized animal in a typical codominant fashion. There is no evidence so far for trans-complementation between low-responder haplotypes. Detailed studies of Ir-RT1Aa indicate that it controls the antibody response to at least two distinct alloantigenic determinants on RT1Aa molecules. These class I molecules thus behave like hapten-carrier conjugates when the response against the carrier is under Ir gene control. Analysis of the origin of alloantibody-forming cells in tetraparental radiation chimeras indicates that Ir-RT1Aa must control the provision of effective help to B cells. In many respects therefore, the properties of Ir-RT1Aa are broadly similar to those described for Ir genes controlling antibody responses to conventional antigens. The existence of apparently conventional Ir genes controlling the antibody response to major alloantigens strongly suggest that the processing of these transmembrane molecules by host antigen-presenting cells is a prerequisite for immune induction, and that it is the MHC of the responder rather than that of the allograft to which T helper cells are restricted in alloimmune responses in vivo.

Variable generation of specific acquired resistance in CBA/Ca mice chronically infected with schistosomiasis mansoni

Parasitology ◽  
1989 ◽  
Vol 99 (3) ◽  
pp. 357-364
Author(s):  
V. S. Delgado ◽  
D. J. Mclaren
Keyword(s):  
Schistosoma Mansoni ◽  
Worm Burden ◽  
Acquired Resistance ◽  
Acquired Immunity ◽  
Infection Model ◽  
Schistosome Infection ◽  
Immune Mechanisms ◽  
Schistosomiasis Mansoni ◽  
Variable Generation ◽  
Worm Migration

SummaryNaive CBA/Ca mice and CBA/Ca mice infected 12 weeks previously with 20 normal cercariae of Schistosoma mansoni were challenged percutaneously with isotopically labelled parasites. Challenge worm migration was followed, in 5 separate experiments, by means of compressed organ autoradiography. In three experiments, 43.1 % of challenge parasites did not arrive in the lungs of infected mice on day 6 as compared to 7.7 % in naive controls, thereby indicating that pre-lung sites constitute the first barrier in resistance to reinfection. A further 15% of the challenge worm burden was lost in the lungs or en route to the liver in the immune animals, and portal perfusion revealed that 25.4% of the challenge was lost in the liver. Two other experiments revealed no comparable phases of pre-liver attrition however; instead resistance was only evident at final perfusion on days 28 or 35. These data reveal the variable generation of specific acquired immunity in mice harbouring a chronic schistosome infection and thus clarify current discrepancies in the literature. The results are discussed in relation to documented evidence for the nature of specific and non-specific immune mechanisms reported to operate at different sites in the infection model of schistosomiasis mansoni.

scholarly journals Two Major Histocompatibility Complex Class I-Restricted Epitopes of the Borna Disease Virus p10 Protein Identified by Cytotoxic T Lymphocytes Induced by DNA-Based Immunization

2003 ◽  
Vol 77 (10) ◽  
pp. 6076-6081 ◽  
Author(s):  
Yoshio Hashimoto ◽  
Horng-Shen Chen ◽  
Cynthia Cunningham ◽  
Tahir H. Malik ◽  
Patrick K. Lai
Keyword(s):  
Major Histocompatibility Complex ◽  
T Lymphocytes ◽  
Antibody Response ◽  
Major Histocompatibility Complex Class ◽  
Disease Virus ◽  
Cytotoxic T Lymphocytes ◽  
Complex Class ◽  
Lewis Rats ◽  
Histocompatibility Complex ◽  
Borna Disease

ABSTRACT Borna disease virus (BDV) infection of Lewis rats is the most studied animal model of Borna disease, an often fatal encephalomyelitis. In this experimental model, BDV-specific CD8+ cytotoxic T lymphocytes (CTLs) play a prominent role in the immunopathogenesis of infection by the noncytolytic, persistent BDV. Of the six open reading frames of BDV, CTLs to BDV X (p10) and the l-polymerase have never been studied. In this study, we used plasmid immunization to investigate the CTL response to BDV X and N. Plasmid-based immunization was a potent CTL inducer in Lewis rats. Anti-X CTLs were primed by a single injection of the p10 cDNA. Two codominant p10 epitopes, M1SSDLRLTLL10 and T8LLELVRRL16, associated with the RT1.Al major histocompatibility complex class I molecules of the Lewis rats, were identified. In addition, immunization with a BDV p40-expressing plasmid confirmed the previously reported RT1.Al-restricted A230SYAQMTTY238 peptide as the CTL target for BDV N. In contrast to the CTL responses, plasmid vaccination was a poor inducer of an antibody response to p10. Three injections of a recombinant eukaryotic expression plasmid of BDV p10 were needed to generate a weak anti-p10 immunoglobulin M response. However, the antibody response could be optimized by a protein boost after priming with cDNA.

scholarly journals Non-Major Histocompatibility Complex Control of Antibody Isotype and Th1 versus Th2 Cytokines during Experimental Infection of Mice with Mycobacterium avium

2001 ◽  
Vol 69 (3) ◽  
pp. 1708-1713 ◽  
Author(s):  
Vijaya Nagabhushanam ◽  
Christina Cheers
Keyword(s):  
Major Histocompatibility Complex ◽  
Antibody Response ◽  
Mycobacterium Avium ◽  
Enzyme Linked Immunosorbent Assay ◽  
Similar Response ◽  
Th2 Cytokines ◽  
Major Histocompatibility ◽  
Major Histocompatibility Complex Haplotype ◽  
Histocompatibility Complex ◽  
Ifn Γ

ABSTRACT Infection of different strains of mice withMycobacterium avium has revealed genetic control of the immunoglobulin isotype induced and of the balance between Th1 and Th2 cytokines. Female BALB/c or C57BL/10 mice were infected intranasally with 105 M. avium organisms. The antibody response was measured over 18 weeks by enzyme-linked immunosorbent assay and Western blotting, while numbers of cytokine-producing cells were assessed at 12 to 15 weeks by ELISPOT assay. Upon infection, C57BL/10 mice produced a clear Th1 response with strong gamma interferon (IFN-γ) production, no interleukin-4 (IL-4), and almost entirely immunoglobulin G2a (IgG2a) antibody. In contrast, BALB/c mice developed T cells producing IL-4, as well as those producing IFN-γ, while the antibody response was a mixture of IgG1 and IgG2a. Antibodies from BALB/c mice were also able to recognize a greater range of antigens than were C56BL/10 mice. B10D2 mice, which carry the BALB/c major histocompatibility complex haplotype on a C57BL/10 background, followed the C57BL/10 cytokine pattern. Mice infected withListeria monocytogenes did not show a similar response dichotomy.

Sign in / Sign up

Export Citation Format

Share Document