Triatoma infestans susceptibility to different Trypanosoma cruzi strains: parasite development and early escape from anterior midgut

Parasitology ◽  
2020 ◽  
pp. 1-7
Author(s):  
Larissa F. Paranaiba ◽  
Rodrigo P. Soares ◽  
Alessandra A. Guarneri
Keyword(s):  
Trypanosoma Cruzi ◽  
Digestive Tract ◽  
Triatoma Infestans ◽  
Parasite Development ◽  
Metacyclic Trypomastigotes ◽  
Posterior Midgut ◽  
Kinetics Of ◽  
Anterior Midgut

Abstract The escape kinetics from the anterior midgut (AM) of Trypanosoma cruzi during the initial steps of infection was assessed in Triatoma infestans, as well as its ability to survive migration in the digestive tract of the vector. All the four strains evaluated survived and reached variable parasite densities. After 49–50 days, YuYu [discrete typing units (DTU) I] strain reached the highest parasite numbers in the rectum followed by Bug (DTU V), CL-Brener (DTU VI) and Dm28c (DTU I). All strains accomplished metacyclogenesis. Bug strain reached the highest numbers of metacyclic trypomastigotes followed by YuYu and CL-Brener/Dm28c. A remarkable parasite reduction in the AM for Bug strain, but not Dm28c was noticed at 72 h of infection. In the posterior midgut + rectum high densities of parasites from both strains were detected at this period indicating the parasites crossed the AM. For Dm28c strain, in infections initiated with trypomastigotes, parasites left AM faster than those starting with epimastigotes. In conclusion, T. cruzi strains from different DTUs were able to infect T. infestans reaching variable parasite densities. The kinetics of migration in the digestive tract may be affected by strain and/or the evolutive form used for infection.

A new antimicrobial protein from the anterior midgut of Triatoma infestans mediates Trypanosoma cruzi establishment by controlling the microbiota

Biochimie ◽  
2016 ◽  
Vol 123 ◽  
pp. 138-143 ◽  
Author(s):  
Diego S. Buarque ◽  
Cícera M. Gomes ◽  
Ricardo N. Araújo ◽  
Marcos H. Pereira ◽  
Roberta C. Ferreira ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Triatoma Infestans ◽  
Antimicrobial Protein ◽  
Anterior Midgut

scholarly journals The Fungal Microbiota of the Digestive Tract of the Chagas Disease Vectors Triatoma infestans Klug, 1834 and Panstrongylus megistus Burmeister, 1835

2020 ◽  
pp. 13-21
Author(s):  
Ingrid dos Santos da Silva ◽  
Mônica de França Guedelha ◽  
Cíntia Alves da Silva ◽  
Lara Cristina Santos ◽  
Angela Cristina Verissimo Junqueira ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Digestive Tract ◽  
Culture Media ◽  
Vector Competence ◽  
Public Health Problem ◽  
Triatoma Infestans ◽  
Nymphal Stage ◽  
Panstrongylus Megistus ◽  
Fungal Strains ◽  
Fungal Microbiota

Chagas is a neglected disease, one of Brazil’s main medical and social problems and a serious public health problem in the Americas, with more recent occurrences in non-endemic countries outside of the Americas. Research into the microbiota of triatomines is relevant because of its potential role in vector competence and as a proposed biological control strategy. Stressing a possible insect-fungal interaction in the development of Trypanosoma cruzi, and considering the lack of studies on the subject, we analyzed the fungal microbiota of the digestive tract of two species considered important vectors of Trypanosoma cruzi: Triatoma infestans and Panstrongylus megistus. Specimens were dissected, digestive tracts macerated and contents serially diluted. Each aliquot was seeded in three culture media. The plates were incubated in type B.O.D. climate chambers for 21 days, after which isolated colonies were morphological characterized and identified. There have been few published studies on the fungal microbiota of the triatomine digestive tract. Comparing the results found here with existing data reveals that the genera Aspergillus and Penicillium are commonly found in the digestive tract of the studied triatomines. Among the several genera identified, the species found in the highest percentages were Aspergillus flavus, Paecilomyces variotii, Penicillium waksmanii, Penicillium raistrickii and Penicillium fellutanum. Quantitative differences in the number of isolated fungal strains were observed according to sex and nymphal stage of the vector. The present findings corroborate those found in the literature, showing that there is a natural fungal microbiota in triatomines. Data revealing quantitative differences in isolated fungal strains found in male, female and nymphs reinforce the idea that their presence is related to physiology and fasting resistance. The secondary metabolite-producing fungi isolated in this work have in their biology great potential to be tested with regard to the establishment of T. cruzi in the digestive tract of its vector.

Colonization of Rhodnius prolixus gut by Trypanosoma cruzi involves an extensive parasite killing

Parasitology ◽  
2016 ◽  
Vol 143 (4) ◽  
pp. 434-443 ◽  
Author(s):  
ROBERTA CARVALHO FERREIRA ◽  
RAFAEL LUIS KESSLER ◽  
MARCELO GUSTAVO LORENZO ◽  
RAFAELA MAGALHÃES MACEDO PAIM ◽  
LUCIANA DE LIMA FERREIRA ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Population Size ◽  
Chagas Disease ◽  
Intestinal Tract ◽  
Parasite Load ◽  
Rhodnius Prolixus ◽  
Insect Vector ◽  
Posterior Midgut ◽  
Development And Differentiation ◽  
Anterior Midgut

SUMMARYTrypanosoma cruzi, the etiological agent of Chagas disease, is ingested by triatomines during their bloodmeal on an infected mammal. Aiming to investigate the development and differentiation of T. cruzi inside the intestinal tract of Rhodnius prolixus at the beginning of infection we fed insects with cultured epimastigotes and blood trypomastigotes from infected mice to determine the amount of recovered parasites after ingestion. Approximately 20% of the ingested parasites was found in the insect anterior midgut (AM) 3 h after feeding. Interestingly, a significant reduction (80%) in the numbers of trypomastigotes was observed after 24 h of infection suggesting that parasites were killed in the AM. Moreover, few parasites were found in that intestinal portion after 96 h of infection. The evaluation of the numbers of parasites in the posterior midgut (PM) at the same periods showed a reduced parasite load, indicating that parasites were not moving from the AM. Additionally, incubation of blood trypomastigotes with extracts from R. prolixus AMs revealed that components of this tissue could induce significant death of T. cruzi. Finally, we observed that differentiation from trypomastigotes to epimastigotes is not completed in the AM; instead we suggest that trypomastigotes change to intermediary forms before their migration to the PM, where differentiation to epimastigotes takes place. The present work clarifies controversial points concerning T. cruzi development in insect vector, showing that parasite suffers a drastic decrease in population size before epimastigonesis accomplishment in PM.

scholarly journals In vitro differentiation of Trypanosoma cruzi epimastigotes into metacyclic trypomastigotes using a biphasic medium

2021 ◽  
Vol 2 (3) ◽  
pp. 100703
Author(s):  
Jessica Rodríguez Durán ◽  
Arturo Muñoz-Calderón ◽  
Karina Andrea Gómez ◽  
Mariana Potenza
Keyword(s):  
Trypanosoma Cruzi ◽  
In Vitro Differentiation ◽  
Metacyclic Trypomastigotes ◽  
Biphasic Medium

scholarly journals Cardiac morbidity due to Chagas' disease in a rural community in Bolivia

1988 ◽  
Vol 101 (3) ◽  
pp. 655-660 ◽  
Author(s):  
Th. Weinke ◽  
K. Ueberrieiter ◽  
M. Alexander
Keyword(s):  
Health Education ◽  
Trypanosoma Cruzi ◽  
Rural Community ◽  
Preventive Measures ◽  
Triatoma Infestans ◽  
Bundle Branch Block ◽  
Cardiac Morbidity ◽  
House Improvement ◽  
Ecg Alterations ◽  
Anterior Hemiblock

SUMMARYThe prevalence of cardiac morbidity due to Change' disease was assessed in a rural community in Central Bolivia. Sixty-nine of 104 persons (66%) were seropositive to Trypanosoma cruzi by two serological methods. Two of 35 (6%) seronegative individuals presented with modest ECG alterations (left anterior hemiblock), but 21 of 69 (30%) seropositives showed modest and severe abnormalities (6 complete right bundle branch block. 2 polyfocal or frequent extrasystoles, 9 ischaemic ST alterations). A high percentage (56%) of domiciliary Triatoma infestans was infected with T. cruzi. There was a significant association between seropositivity and substandard housing. Priority preventive measures should thus include house improvement (to reduce bug infestation) and health education.

scholarly journals Incapacidade de evolução do Trypanosoma cruzi na hemocele de triatomíneos

1982 ◽  
Vol 77 (4) ◽  
pp. 431-436 ◽  
Author(s):  
Nelson J. Alvarenga ◽  
Elizabeth Bronfen
Keyword(s):  
Trypanosoma Cruzi ◽  
Triatoma Infestans

O exame da hemolinfa de triatomíneos (Triatoma infestans e Dipetalogastermaxima) infectados pelo Trypanosoma cruzi há 10, 15, 20 e 30 dias não revelaram a presença do flagelado. Material colhido e lavado da hemocele de D. maxima infectados, também foi negativo. A inoculação de formas sanguíneas do parasita e daquelas obtidas do conteúdo intestinal de triatomíneos na hemocele revelou que os parasitas não foram capazes de manter na hemolinfa uma infecção por mais de 40 dias e que não puderam penetrar no intestino dos triatomíneos. A inoculação de hemolinfa de insetos naturalmente infectados em camundongos recém-nascidos não induziu infecção.

Sign in / Sign up

Export Citation Format

Share Document