The Effect of Siduron Upon Barley Root Metabolism

Weed Science ◽  
1968 ◽  
Vol 16 (3) ◽  
pp. 344-347 ◽  
Author(s):  
Walter E. Splittstoesser

Barley (Hordeum vulgareL. var. Trail) root growth was inhibited at lower concentrations of 1-(2-methylcyclohexyl)-3-phenylurea (siduron) than was shoot growth. The influence of siduron upon root metabolism was assessed with excised roots grown in 0 or 5 ppm siduron. More glucose-U-14C and leucine-U-14C were degraded to CO2and less were incorporated into cell wall material and protein by roots grown in siduron. However, roots grown in siduron incorporated more adenine-8-14C into nucleic acids and degraded less adenine to CO2than roots grown in water. It was suggested that siduron disrupted the normal nucleic acid metabolism of barley roots which was necessary for protein and cell wall synthesis.

1959 ◽  
Vol 5 (6) ◽  
pp. 641-648 ◽  
Author(s):  
R. G. E. Murray ◽  
W. H. Francombe ◽  
B. H. Mayall

Cultures of sensitive stains of Staphylococcus aureus were fixed with osmium tetroxide after 1–5 hours' exposure to various does of pencillin and were embedded in methacrylate for sectioning and electron microscopy. They were compared with untreated, control cultures. The contrast of the cell wall material was untreated, control cultures. The contrast of the cell wall material was increased, by cutting the section of lanthanum nitrate.The cells increased in size and the surrounding cell wall was thinner than normal. The main lesions appeared in the developing cell wall septa, which showed a loss in density and gross irregularity of shape. Some questionable inclusions were seen in the cytoplasm. Lysis was prevented in a medium containing 0.3 M sucrose and the stable spheroplasts retained a recognizable cell wall after 24 hours' exposure to penicillin. However, the septa could not be demonstrated in the cells treated in sucrose medium.Two resistant strains were exposed to penicillin. In one, the cells showed no morphological effects; in the other, there was temporary damage to the cell septa with complete recovery.The observations support the hypothesis that penicillin interferes with the synthesis of a cell wall component and indicate that the main point of cell wall synthesis is at the site of septum formation.


1963 ◽  
Vol 9 (3) ◽  
pp. 337-341 ◽  
Author(s):  
MICHIO OISHI ◽  
SHIGERU KITAYAMA ◽  
HAJIME TAKAHASHI ◽  
BUNJI MARUO

2018 ◽  
Vol 15 (8) ◽  
pp. 513
Author(s):  
Ewen Silvester ◽  
Annaleise R. Klein ◽  
Kerry L. Whitworth ◽  
Ljiljana Puskar ◽  
Mark J. Tobin

Environmental contextSphagnum moss is a widespread species in peatlands globally and responsible for a large fraction of carbon storage in these systems. We used synchrotron infrared microspectroscopy to characterise the acid-base properties of Sphagnum moss and the conditions under which calcium uptake can occur (essential for plant tissue integrity). The work allows a chemical model for Sphagnum distribution in the landscape to be proposed. AbstractSphagnum is one the major moss types responsible for the deposition of organic soils in peatland systems. The cell walls of this moss have a high proportion of carboxylated polysaccharides (polygalacturonic acids), which act as ion exchangers and are likely to be important for the structural integrity of the cell walls. We used synchrotron light source infrared microspectroscopy to characterise the acid-base and calcium complexation properties of the cell walls of Sphagnum cristatum stems, using freshly sectioned tissue confined in a flowing liquid cell with both normal water and D2O media. The Fourier transform infrared spectra of acid and base forms are consistent with those expected for protonated and deprotonated aliphatic carboxylic acids (such as uronic acids). Spectral deconvolution shows that the dominant aliphatic carboxylic groups in this material behave as a monoprotic acid (pKa=4.97–6.04). The cell wall material shows a high affinity for calcium, with a binding constant (K) in the range 103.9–104.7 (1:1 complex). The chemical complexation model developed here allows for the prediction of the chemical environment (e.g. pH, ionic content) under which Ca2+ uptake can occur, and provides an improved understanding for the observed distribution of Sphagnum in the landscape.


1968 ◽  
Vol 259 (2) ◽  
pp. 186-188 ◽  
Author(s):  
D. Neubert ◽  
E. Oberdisse ◽  
H.-J. Merker ◽  
E. K�hler ◽  
B.-R. Balda

1966 ◽  
Vol 241 (12) ◽  
pp. 2876-2880 ◽  
Author(s):  
Hubert S. Loring ◽  
J.E. McLennan ◽  
Tom L. Walters

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