Abstract. In situ measurements in the upper troposphere – lower stratosphere (UTLS) are performed in the framework of the European research infrastructure IAGOS (In-service Aircraft for a Global Observing System) for ozone since 1994 and for carbon monoxide since 2002. The flight tracks cover a wide range of longitudes in the northern extratropics, extending from the North American western coast (125° W) to the eastern Asian coast (135° E), and more recently over the northern Pacific ocean. Different tropical regions are also sampled frequently, such as the Brazilian coast, central and southern Africa, southeastern Asia and the western Maritime Continent. As a result, a new set of climatologies for O3 (Aug. 1994–Dec. 2013) and CO (Dec. 2001–Dec. 2013) in the upper troposphere (UT), tropopause layer and lower stratosphere (LS) are made available, including quasi-global gridded horizontal distributions, and seasonal cycles over eight well sampled regions of interest in the northern extratropics. The seasonal cycles generally show a summertime maximum in O3 and a springtime maximum in CO in the UT, in contrast with the systematic springtime maximum in O3 and the quasi-absence of seasonal cycle of CO in the LS. This study highlights some regional variabilities in the UT notably (i) a west-east difference of O3 in boreal summer with up to 15 ppb more O3 over central Russia compared with northeast America, (ii) a systematic west-east gradient of CO from 60° E to 140° E (especially noticeable in spring and summer with about 5 ppb by 10 degrees longitude), (iii) a broad spring/summer maximum of CO over North East Asia, and (iv) a spring maximum of O3 over Western North America. Thanks to almost 20 years of O3 and 12 years of CO measurements, the IAGOS database is a unique data set to derive trends in the UTLS. Trends in O3 in the UT are positive and statistically significant in most regions, ranging from +0.25 to +0.45 ppb yr−1, characterized by the significant increase of the lowest values of the distribution. No significant trends of O3 are detected in the LS. Trends of CO in the UT, tropopause and LS are all negative and statistically significant. The estimated slopes range from −1.37 to −0.59 ppb yr−1 , with a nearly homogeneous decrease of the lowest values of the monthly distribution (fifth percentile) contrasting with the high inter-regional variability of the highest values (95th percentile).