scholarly journals A Four Star Photometer

1993 ◽  
Vol 136 ◽  
pp. 179-186
Author(s):  
J.C. Valtier ◽  
J.M. Le Contel ◽  
P. Antonelli ◽  
P. Michel ◽  
J.P. Sareyan
Keyword(s):  
Real Time ◽  
Focal Plane ◽  
Ccd Camera ◽  
Simultaneous Acquisition ◽  
Comparison Stars

AbstractA new photometer is presently being developed at the O.C.A. Observatory. It consists of four arms and a CCD camera situated in the focal plane of the telescope. Each arm can move in both directions and support a diaphragm and a liquid optic guide that directs the light to a photomultiplier. The simultaneous acquisition of the four signals enables to obtain magnitude differences between the objects in real time. A typical use of this photometer is to observe at the same time one or two variables, comparison stars and the sky background.

scholarly journals Multi-Modal Multi-Spectral Intravital Microscopic Imaging of Signaling Dynamics in Real-Time during Tumor–Immune Interactions

Cells ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 499
Author(s):  
Tracy W. Liu ◽  
Seth T. Gammon ◽  
David Piwnica-Worms
Keyword(s):  
Molecular Imaging ◽  
Single Cell ◽  
Real Time ◽  
Bioluminescence Imaging ◽  
Emission Spectra ◽  
Ccd Camera ◽  
Microscopic Imaging ◽  
Signaling Dynamics ◽  
Window Chamber

Intravital microscopic imaging (IVM) allows for the study of interactions between immune cells and tumor cells in a dynamic, physiologically relevant system in vivo. Current IVM strategies primarily use fluorescence imaging; however, with the advances in bioluminescence imaging and the development of new bioluminescent reporters with expanded emission spectra, the applications for bioluminescence are extending to single cell imaging. Herein, we describe a molecular imaging window chamber platform that uniquely combines both bioluminescent and fluorescent genetically encoded reporters, as well as exogenous reporters, providing a powerful multi-plex strategy to study molecular and cellular processes in real-time in intact living systems at single cell resolution all in one system. We demonstrate that our molecular imaging window chamber platform is capable of imaging signaling dynamics in real-time at cellular resolution during tumor progression. Importantly, we expand the utility of IVM by modifying an off-the-shelf commercial system with the addition of bioluminescence imaging achieved by the addition of a CCD camera and demonstrate high quality imaging within the reaches of any biology laboratory.

scholarly journals Real-Time Depth From Focus on a Programmable Focal Plane Processor

2018 ◽  
Vol 65 (3) ◽  
pp. 925-934 ◽  
Author(s):  
Julien N. P. Martel ◽  
Lorenz K. Muller ◽  
Stephen J. Carey ◽  
Jonathan Muller ◽  
Yulia Sandamirskaya ◽  
...  
Keyword(s):  
Real Time ◽  
Focal Plane ◽  
Depth From Focus

scholarly journals T A U M O K—Multi-Object Spectroscopy with the Tautenburg Schmidt Telescope

1995 ◽  
Vol 148 ◽  
pp. 52-52
Author(s):  
S. Marx ◽  
H. Lehmann ◽  
M. Pluto ◽  
R. Ziener
Keyword(s):  
Focal Plane ◽  
Ccd Camera ◽  
Entrance Slit ◽  
Pixel Size ◽  
Schmidt Telescope ◽  
Max Planck ◽  
Background Light ◽  
Convex Lens ◽  
Chip Size ◽  
Calar Alto

A multi-object camera for the Tautenburg Schmidt Telescope (134/200/400) was developed and constructed in cooperation with the Max-Planck-Institut für Astronomie in Heidelberg and the Thüringer Landesstemwarte Tautenburg. The experience with the “Spaltspinne” for the 3.5 m Telescope of the Calar Alto Observatory (Pitz 1993) was the foundation of TAUMOK. The technical concept was given by Pitz et al. (1993).Thirty six rods controlled by a processor can be moved in an area with a diameter of 16 cm. This area covers a field of 2.3° in the focal plane which is flattened by a plano-convex lens. Each of 34 rods carry two fibres with diameters of 100/µm with one fibre for the object and another for the background light. The fibres have a length of 7 m and their opposite faces form the entrance slit of a spectrograph. At present, reciprocal dispersions of 3.4, 10, 20 and 40 nm/mm can be realized. A CCD camera with a chip-size of 1152 × 770 pixels (pixel size 22.5/µm) is used as detector. Two of the 36 rods are equipped with image bundles for telescope guiding.

A Real-Time Continuous Flow Polymerase Chain Reactor for DNA Expression Quantification

2007 ◽  
Author(s):  
Michael B. Sayers ◽  
Tara M. Dalton
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Quantitative Pcr ◽  
Real Time Pcr ◽  
Continuous Flow ◽  
Quantitative Polymerase Chain Reaction ◽  
Ccd Camera ◽  
Fluorescent Imaging ◽  
Intensity Level ◽  
Polymerase Chain

Real-time quantitative Polymerase Chain Reaction (PCR) is an extremely sensitive and reliable method for quantifying gene expression, allowing subtle shifts in gene expression to be easily monitored. Currently, stationary real-time PCR is readily achieved using fluorescent labels which increase in fluorescence as the DNA is exponentially amplified. Quantitative PCR is used in a myriad of applications. However currently most commercial real-time PCR devices are batch process stationary well based systems, limiting their throughput. Continuous flow microfluidic PCR devices have allowed for advancement in terms of improved PCR throughput and reduced reagent usage. As part of an overall total analysis system a device integrating all the functional steps of continuous flow realtime quantitative PCR has been designed and fabricated. Initially the PCR reaction mixture is segmented into nano-litre PCR reactors which are then thermally cycled on a two temperature fifty cycle flow-through PCR device, which allows laser induced fluorescent imaging of the nanoreactors. Previous studies into continuous flow PCR have demonstrated endpoint fluorescent measurements, however this research allows PCR nanoreactors to be fluorescently monitored after every PCR thermal cycle. Fluorescent optical monitoring is achieved through laser excitation of the nanoreactors while a Charged Coupled Device (CCD) camera is used to record the fluorescent emissions from the nanoreactors. Intensity analysis of the recorded images is then preformed using MATLAB to accurately determine the fluorescence intensity level, thereby allowing real-time quantitative amplification curves to be generated. This has major advantages over existing continuous flow PCR devices which use endpoint fluorescence and capillary electrophoresis, as the amplification curves allow far more information to be gleaned and allow the initial DNA template concentration to be accurately determined.

scholarly journals MOCAM: A 4k × 4k CCD Mosaic for the Canada-France-Hawaii Telescope Prime Focus

1995 ◽  
Vol 167 ◽  
pp. 213-220
Author(s):  
J. C. Cuillandre ◽  
Y. Melliers ◽  
R. Murowinski ◽  
D. Crampton ◽  
G. Luppino ◽  
...  
Keyword(s):  
Focal Plane ◽  
San Diego ◽  
Ccd Camera ◽  
Astronomical Observatory ◽  
Wide Field ◽  
On Line ◽  
Readout Noise ◽  
The University ◽  
Filter Wheel ◽  
Better Than

MOCAM is a wide field CCD camera, currently nearing completion, which will be offered to the Canada-France-Hawaii Telescope (CFHT) user community in 1995. The project is a collaboration between the CFHT, the Dominion Astronomical Observatory (DAO, Canada), the Institut des Sciences de l'Univers (INSU, France), Laboratoire d'Astrophysique de Toulouse (LAT, France) and the University of Hawaii (UH). In the interests of producing a reliable and effective camera in the shortest time, it was decided to use existing technologies rather than innovative ones. Two-edge buttable 2048 × 2048 15 μm pixel CCDs were obtained from the LORAL aerospace foundry, based on a mask designed by J. Geary at Smithsonian Astrophysical Observatory (SAO). They are mounted in a dewar designed by G. Luppino (UH); the focal plane mounting keeps the mosaic flat to within two pixels and the CCDs are aligned to within two pixels. A mechanical interface designed and fabricated by the DAO holds a 150 mm shutter and a filter wheel which has a positioning repeatability better than five μm.The four CCDs are operated in parallel by a San Diego GenIII controller adapted by LAT. The mosaic is read out in seven minutes and a single 33 Mb FITS file is generated to enable convenient on-line preprocessing. The user will control the system through a single CFHT Pegasus environment session. The camera field is 14′ × 14′ with a 0.″2 pixel sampling and the readout noise is less than seven electrons. The scientific goals of the initiators of the project are studies of distant clusters, deep galaxy counts and quasars surveys.

Measurement on roughness of optical surface by focal plane CCD camera

1998 ◽  
Author(s):  
Jianbai Li ◽  
Xiaoyun Li ◽  
Aihan Ying ◽  
Anqing Zao ◽  
Xiaolin Zhang
Keyword(s):  
Focal Plane ◽  
Ccd Camera ◽  
Optical Surface

Metamaterial-integrated Non-electric Focal Plane Array for Real-time Terahertz Imaging

2017 ◽  
Author(s):  
Yongzheng Wen ◽  
Delin Jia ◽  
Wei Ma ◽  
Yun Feng ◽  
Ming Liu ◽  
...  
Keyword(s):  
Real Time ◽  
Focal Plane ◽  
Focal Plane Array ◽  
Terahertz Imaging
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