Expression of igf-i and gh-receptor (ghr) mrna in liver and skeletal muscle of different pig breeds

1995 ◽  
Vol 1995 ◽  
pp. 183-183
Author(s):  
J.M. Brameld ◽  
J.L. Atkinson ◽  
T.J. Budd ◽  
J.C. Saunders ◽  
A.M. Salter ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Energy Availability ◽  
Pig Breeds ◽  
Gh Receptor ◽  
Age Related ◽  
Igf I ◽  
Class 1 ◽  
Cellular Domain ◽  
Promoter Usage ◽  
Age Related Changes

Previous studies of the porcine GH-IGF axis have demonstrated age related changes in liver, but not LD muscle, in expression of both GH-receptor (GHR) and IGF-I genes (Brameld et al, 1993; 1995), and also effects of energy availability on the expression of IGF-I in liver, but not in LD muscle (Weller et al, 1994). The work described here was carried out to study the expression of IGF-I and GHR mRNA in liver and skeletal muscle from different breeds of pig. The probes used were as described previously (Brameld et al, 1993; 1995; Weller et al, 1993; 1994), and were designed to monitor promoter usage for IGF-I expression, with the two promoters giving rise to class 1 and 2 transcripts, and also expression of the extra-cellular domain of the GH-receptor.

Expression of igf-i and gh-receptor (ghr) mrna in liver and skeletal muscle of different pig breeds

1995 ◽  
Vol 1995 ◽  
pp. 183-183
Author(s):  
J.M. Brameld ◽  
J.L. Atkinson ◽  
T.J. Budd ◽  
J.C. Saunders ◽  
A.M. Salter ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Energy Availability ◽  
Pig Breeds ◽  
Gh Receptor ◽  
Age Related ◽  
Igf I ◽  
Class 1 ◽  
Cellular Domain ◽  
Promoter Usage ◽  
Age Related Changes

Previous studies of the porcine GH-IGF axis have demonstrated age related changes in liver, but not LD muscle, in expression of both GH-receptor (GHR) and IGF-I genes (Brameld et al, 1993; 1995), and also effects of energy availability on the expression of IGF-I in liver, but not in LD muscle (Weller et al, 1994). The work described here was carried out to study the expression of IGF-I and GHR mRNA in liver and skeletal muscle from different breeds of pig. The probes used were as described previously (Brameld et al, 1993; 1995; Weller et al, 1993; 1994), and were designed to monitor promoter usage for IGF-I expression, with the two promoters giving rise to class 1 and 2 transcripts, and also expression of the extra-cellular domain of the GH-receptor.

Ontogenic study of Expression of IGF-I and Gh-Receptor (GHR) mRNA in pig Liver and Skeletal Muscle.

1993 ◽  
Vol 1993 ◽  
pp. 151-151
Author(s):  
J.M. Brameld ◽  
P.A. Weller ◽  
R.S. Gilmour ◽  
P.J. Buttery
Keyword(s):  
Skeletal Muscle ◽  
Cell Types ◽  
Muscle Fibres ◽  
Alternative Promoters ◽  
Pig Liver ◽  
Gh Receptor ◽  
Age Related ◽  
Igf I ◽  
Class 1 ◽  
Stimulation Of

Many of the effects of growth hormone are now thought to be mediated via the stimulation of insulin-like growth factor-I (IGF-I) production by many tissues, especially the liver, with this stimulation being dependent upon the presence of the GH-receptor (GHR). IGF-I gene expression occurs via alternative promoters giving rise to class 1 and 2 transcripts, of which class 2 is thought to be preferentially responsive to GH (Saunders, Dickson, Pell & Gilmour, 1991).The effects of IGF-I include the stimulation of DNA synthesis (mitogenesis) and protein synthesis in most cell types, together with the differentiation of many cell types into mature tissue, including the differentiation of muscle cells into muscle fibres. Thus the GH/IGF-I axis has been found to play a major part in the control of animal growth. For this reason, we studied the age related changes in IGF-I and GHR mRNA expression in pig liver and skeletal muscle.

Understanding Age-Related Changes in Skeletal Muscle Metabolism: Differences Between Females and Males

2016 ◽  
Vol 36 (1) ◽  
pp. 129-156 ◽  
Author(s):  
Brandon J.F. Gheller ◽  
Emily S. Riddle ◽  
Melinda R. Lem ◽  
Anna E. Thalacker-Mercer
Keyword(s):  
Skeletal Muscle ◽  
Muscle Metabolism ◽  
Skeletal Muscle Metabolism ◽  
Age Related ◽  
Age Related Changes

Age-related changes in glutathione availability and skeletal muscle carbonyl content in healthy rats

2004 ◽  
Vol 39 (2) ◽  
pp. 203-210 ◽  
Author(s):  
Laurent Mosoni ◽  
Denis Breuillé ◽  
Caroline Buffière ◽  
Christiane Obled ◽  
Philippe Patureau Mirand
Keyword(s):  
Skeletal Muscle ◽  
Carbonyl Content ◽  
Age Related ◽  
Age Related Changes

The interrelationship between and the regulation of hepatic growth hormone receptors and circulating GH binding protein in the pig

1992 ◽  
Vol 126 (2) ◽  
pp. 155-161 ◽  
Author(s):  
Geoffrey R Ambler ◽  
Bernhard H Breier ◽  
Andrzej Surus ◽  
Hugh T Blair ◽  
Stuart N McCutcheon ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Growth Hormone Receptor ◽  
Hormone Receptors ◽  
Binding Capacity ◽  
Binding Protein ◽  
Somatic Growth ◽  
Gh Receptor ◽  
Age Related ◽  
Igf I ◽  
Serum Gh

We evaluated the interrelationship between, and regulation of, the hepatic growth hormone receptor and serum GH binding protein (GH BP) in pigs treated with recombinant porcine growth hormone (rpGH). Infant and pubertal male pigs (N = 5 per group) received either rpGH 0.15 mg/kg daily or diluent intramuscularly for 12 days. Somatic growth, serum IGF-I and GH BP and [125I]bovine GH (bGH) binding to MgCl2-treated hepatic membrane homogenates were examined. Marked age-related increases were seen in serum GH BP (p<0.001) and [125I]bGH binding to hepatic membranes (p<0.001). GH BP was increased in rpGH treated animals (p = 0.03), from 13.8±1.2 (mean±1 x sem) (controls) to 17.8±2.0% in infants, and from 35.2±2.6 (controls) to 41.8±3.4% in pubertal animals. [125I]bGH binding to hepatic membranes was also increased by rpGH treatment (p<0.05), from 7.0±1.6 (controls) to 15.4±3.6% in infants and from 53.7±7.1 (controls) to 65.1±11.8% in pubertal animals. No significant interaction between age and treatment was seen. Overall, serum GH BP correlated significantly with [125I]bGH membrane capacity (r=0.82, p<0.001), with a correlation of r= 0.83 in the infant animals but no significant correlation in the pubertal animals considered alone (r=0.13). Serum IGF-I correlated significantly with serum GH BP (r=0.93, p<0.001) and [125]bGH membrane binding capacity (r = 0.91, p< 0.001). These observations suggest that serum GH BP levels reflect major changes of hepatic GH receptor status. In addition, the present study demonstrates that the hepatic GH receptor can be induced by GH in the infant pig, despite a developmentally low GH receptor population at this age, suggesting potential efficacy of GH at earlier ages than generally considered.

scholarly journals Age-related changes in skeletal muscle: changes to life-style as a therapy

2018 ◽  
Vol 19 (6) ◽  
pp. 519-536 ◽  
Author(s):  
Rachel McCormick ◽  
Aphrodite Vasilaki
Keyword(s):  
Skeletal Muscle ◽  
Life Style ◽  
Age Related ◽  
Muscle Changes ◽  
Age Related Changes

scholarly journals Age-related changes in collagen synthesis and degradation in rat tissues. Importance of degradation of newly synthesized collagen in regulating collagen production

1991 ◽  
Vol 276 (2) ◽  
pp. 307-313 ◽  
Author(s):  
P K Mays ◽  
R J McAnulty ◽  
J S Campa ◽  
G J Laurent
Keyword(s):  
Skeletal Muscle ◽  
Collagen Synthesis ◽  
Rat Tissues ◽  
Age Related ◽  
Developmental Growth ◽  
Cross Links ◽  
Fractional Synthesis ◽  
Synthesis And Degradation ◽  
Age Related Changes

During developmental growth, collagens are believed to be continuously deposited into an extracellular matrix which is increasingly stabilized by the formation of covalent cross-links throughout life. However, the age-related changes in rates of synthetic and degradative processes are less well understood. In the present study we measured rates of collagen synthesis in vivo using a flooding dose of unlabelled proline given with [14C]proline and determining production of hydroxy[14C]proline. Degradation of newly synthesized collagen was estimated from the amount of free hydroxy [14C]proline in tissues 30 min after injection. Collagen fractional synthesis rates ranged from about 5%/day in skeletal muscle to 20%/day in hearts of rats aged 1 month. At 15 months of age, collagen fractional synthesis rates had decreased markedly in lung and skin, but in skeletal muscle and heart, rates were unchanged. At 24 months of age, synthesis rates had decreased by at least 10-fold in all tissues, compared with rates at 1 month. The proportion of newly synthesized collagen degraded ranged from 6.4 +/- 0.4% in skin to 61.6 +/- 5.0% in heart at 1 month of age. During aging the proportion degraded increased in all tissues to maximal values at 15 months, ranging from 56 +/- 7% in skin to 96 +/- 1% in heart. These data suggest that there are marked age-related changes in rates of collagen metabolism. They also indicate that synthesis is active even in old animals, where the bulk of collagens produced are destined to be degraded.

Regulation of porcine insulin-like growth factor I and growth hormone receptor mRNA expression by energy status

1994 ◽  
Vol 266 (5) ◽  
pp. E776-E785 ◽  
Author(s):  
P. A. Weller ◽  
M. J. Dauncey ◽  
P. C. Bates ◽  
J. M. Brameld ◽  
P. J. Buttery ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Growth Factor ◽  
Growth Rates ◽  
Mrna Levels ◽  
Energy Status ◽  
Factor I ◽  
Receptor Mrna ◽  
Gh Receptor ◽  
Igf I ◽  
Class 1

Regulation of insulin-like growth factor I (IGF-I) and growth hormone (GH) receptor mRNA in liver and muscle by energy status was assessed in 2-mo-old pigs by altering thermoregulatory demand and energy intake over a 5-wk period to produce a range of plasma IGF-I concentrations from 3.5 +/- 0.7 to 28.9 +/- 6.2 nmol/l. These values were related directly to growth rates (0.06 +/- 0.02 to 0.44 +/- 0.01 kg/day) and total hepatic IGF-I mRNA levels. Increased growth rates were accompanied by an increase in hepatic class 1 and class 2 IGF-I mRNA levels and an increase in the ratio of class 2 to class 1 IGF-I mRNA in liver, suggesting a distinct role for class 2 expression in the endocrine growth response. High levels of class 1 transcripts and a virtual absence of class 2 transcripts characterized all muscle tissues examined, and there was no correlation with plasma IGF-I levels. This suggests that growth promotion in response to increased energy status is regulated via endocrine hepatic IGF-I rather than via a paracrine response. The levels of GH receptor mRNA were positively correlated with overall growth rate (P < 0.005) in liver and negatively correlated (P < 0.05) in muscle, indicating distinct tissue-specific effects of energy status.

scholarly journals The Relation of Inflammaging With Skeletal Muscle Properties in Elderly Men

2019 ◽  
Vol 13 (2) ◽  
pp. 155798831984193 ◽  
Author(s):  
Agnieszka Zembron-Lacny ◽  
Wioletta Dziubek ◽  
Edyta Wolny-Rokicka ◽  
Grazyna Dabrowska ◽  
Marek Wozniewski
Keyword(s):  
Body Composition ◽  
Growth Factors ◽  
Older Men ◽  
Knee Extension ◽  
Functional Fitness ◽  
Muscle Properties ◽  
Walking Test ◽  
Age Related ◽  
Igf I ◽  
Age Related Changes

Aging is associated with a progressive decline of muscle mass and/or the qualitative impairment of the muscle tissue. There is growing evidence of the prominent role of low-grade chronic inflammation in age-related changes in the neuromuscular system. The purpose of the study was to identify the inflammatory mediators responsible for deficit in functional fitness and to explain whether inflammation is related to changes in body composition and the decline of muscle strength in older men. Thirty-three old-aged males (73.5 ± 6.3 years) and twenty young-aged males (21.2 ± 1.3 years) participated in the study. The body composition (bioelectrical impedance analysis), functional capacity (6-min walking test) and knee extension strength (isokinetic test) were estimated. In serum, circulating inflammatory markers H2O2, IL-1β, TNFα, and hsCRP as well as growth factors IGF-I and PDGFBB concentrations were determined (immunoenzymatic methods). The concentrations of H2O2, IL-1β, TNFα, and hsCRP were significantly higher in older than young men. The growth factors IGF-I and PDGFBB were twofold lower and related to high levels of IL-1β and TNFα in the elderly. The changes in cytokines and growth factors levels were correlated with age and peak torque (TQ at 60°/s and 180°/s) in the knee extension. The result of the 6-min walking test was inversely correlated with fat mass index (FMI, r = −.983; p < .001). The generation of inflammatory mediators in older men was related to changes in body composition, maximum strength muscle, and age-related changes in skeletal muscle properties responsible for deficit in functional fitness.

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