Surface Ultrastructure of Gram (−) Bacteria: II. Determination of the Gram Reaction in Antartic Soil Bacteria by Electron Microscopy

1974 ◽  
Vol 32 ◽  
pp. 178-179
Author(s):  
I. L. Uydess ◽  
Wolf Vishniac
Keyword(s):  
Staining Method ◽  
Staining Technique ◽  
Culture Conditions ◽  
Gram Stain ◽  
Accurate Identification ◽  
Surface Ultrastructure ◽  
Antarctic Soils ◽  
Cell Age ◽  
Pure Cultures

A modified negative staining technique (“OTPT”) has been described in the study of the surface topography of bacteria. This technique is useful in the rapid and accurate identification and discrimination of the Gram reaction of unknown bacteria under circumstances unfavorable to the direct application of the Gram stain. Cells of a particular genus exhibit the same basic topographical features independent of cell age, culture conditions or other cellular parameters.Several pure cultures of unclassified bacteria isolated from Antarctic soils were prepared by the OTPT staining method. Although initial attempts employing the Gram stain were often inconclusive, this technique allowed a positive identification of all samples. Two organisms were chosen for more detailed study.

scholarly journals A combined AgNOR-Feulgen staining technique.

1990 ◽  
Vol 38 (11) ◽  
pp. 1591-1597 ◽  
Author(s):  
J Foucrier ◽  
J P Rigaut ◽  
D Pechinot
Keyword(s):  
Transcriptional Activity ◽  
Staining Method ◽  
Staining Technique ◽  
Nucleolus Organizer ◽  
Optimal Conditions ◽  
Feulgen Staining ◽  
Rdna Genes ◽  
Nucleolus Organizer Regions ◽  
Agnor Staining

We describe a new staining technique (H-Ag-S) which allows observation and counting of active nucleolus organizer regions (NORs) and evaluation of the amount of DNA in the same cell nucleus. The procedure consists of combining a modified AgNOR staining method with the Feulgen reaction. A sequential procedure is proposed, based on the determination of optimal staining conditions. The technique, which was designed to allow studies of correlations between the transcriptional activity of rDNA genes and the cell ploidy, was primarily developed for rat liver smears. It should be applicable to most biological preparations, but the optimal conditions might be variable.

scholarly journals Coherent fluctuation nephelometry in clinical microbiology

2019 ◽  
Vol 9 (2) ◽  
pp. 385-392
Author(s):  
A. S. Gur’ev ◽  
O. Yu. Shalatova ◽  
E. V. Rusanova ◽  
I. V. Vasilenko ◽  
A. Yu. Volkov
Keyword(s):  
Antibiotic Susceptibility ◽  
Clinical Microbiology ◽  
Urine Analysis ◽  
Growth Curves ◽  
High Sensitivity ◽  
Pure Cultures ◽  
Susceptibility Tests ◽  
Express Methods ◽  
Coherent Fluctuation

In this article data concerning coherent fluctuation nephelometry (CFN) use in clinical microbiology is presented. CFN-analyzer allows to solve two important problems – fast urine screening for bacteriuria within 2-4 hours and antibiotic susceptibility testing within 3-6 hours. Altogether more than 650 urine samples were tested, and the effectivity of CFN-analyzer for preliminary selection of samples for further analysis was shown. Method allows to detect negative samples, reducing the number of urine analyses by 70-80%. Simultaneous analysis of growth curves and concentration of microorganisms shows high sensitivity and specificity (95.2% и 96.9%). Also more than 250 antibiotic susceptibility tests were performed using CFN-analyzer to show its effectiveness for determination of resistant properties of both pure cultures and urine microflora without isolation of bacteria. The agreement with traditional methods was from 84% to 88%. The use of CFN-analyzer with express methods of identification of microorganisms (chromogenic nutrient broths or mass-spectrometry) allows to make full urine analysis within 1-2 days. In the future CFN-analyzer gives an opportunity to screen different human biological liquids, and finds an application for other microbiological tasks, including standardization and speeding-up in sanitary bacteriology.

Adaptation of the ASTRIN staining technique for quantitative determination of the functional state of the cellular nucleus

1987 ◽  
Vol 81 (1) ◽  
pp. 95-IN2 ◽  
Author(s):  
Péter Kovács ◽  
György Csaba ◽  
László Kőhidai ◽  
Ottilie Török
Keyword(s):  
Quantitative Determination ◽  
Functional State ◽  
Staining Technique

Determination of the Arabian Sand Gazelle Sperm and Acrosomes Defects by Using the Spermac Staining Technique

2011 ◽  
Vol 4 (2) ◽  
pp. 138-147 ◽  
Author(s):  
Al-Eissa Mohammed S ◽  
A.R. Alhamidi ◽  
Saad Alkahtani ◽  
Mohamed Abd El-Kader Sandouka
Keyword(s):  
Staining Technique

Determination of Aphids in Hops

1967 ◽  
Vol 50 (3) ◽  
pp. 499-501
Author(s):  
R G Wright
Keyword(s):  
Staining Technique ◽  
Iodine Staining ◽  
Counting Procedure

Abstract The flotation procedure for determining aphids in hops has been improved over the previously described method (Method A, This Journal, 45, 670 (1962)) by the addition of an iodine staining technique to increase visibility of the aphids and a change in the counting procedure to include, as a whole aphid, any aphid part containing a head

A method for the design of 3D scaffolds for high-density cell attachment and determination of optimum perfusion culture conditions

2008 ◽  
Vol 41 (7) ◽  
pp. 1436-1449 ◽  
Author(s):  
Christophe Provin ◽  
Kiyoshi Takano ◽  
Yasuyuki Sakai ◽  
Teruo Fujii ◽  
Ryo Shirakashi
Keyword(s):  
Cell Attachment ◽  
Perfusion Culture ◽  
Culture Conditions ◽  
High Density ◽  
3D Scaffolds ◽  
High Density Cell

Simultaneous determination of gene expression and bacterial identity in single cells in defined mixtures of pure cultures.

1997 ◽  
Vol 63 (9) ◽  
pp. 3698-3702 ◽  
Author(s):  
L K Poulsen ◽  
H M Dalton ◽  
M L Angles ◽  
K C Marshall ◽  
S Molin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Simultaneous Determination ◽  
Single Cells ◽  
Pure Cultures

The Use of Aniline Blue for the Determination of Dead Phytoplankton, Zooplankton and Meroplankton in LC50 Testings After 96 Hours… A Re-Evaluation of the US Environmental Protection Agency Methodology

2004 ◽  
Vol 126 (3) ◽  
pp. 215-218 ◽  
Author(s):  
Balram Seepersad ◽  
Kelvin Ramnath ◽  
Shyam Dyal ◽  
Reeza Mohammed
Keyword(s):  
Environmental Protection ◽  
Environmental Protection Agency ◽  
Staining Technique ◽  
Aniline Blue ◽  
Protection Agency ◽  
Lc50 Value ◽  
The Us ◽  
Visual Observations

There is a need for a reliable staining technique to distinguish between live and dead organisms following LC50 tests. This is especially so in cases where organisms can be stressed or even become unconscious and appear dead to the aided or naked eyes. Visual observations under such conditions can result in an LC50 value shifting to the lower concentration thereby imposing stiffer guidelines for compliance. Aniline blue can only stain individuals which are physiologically dead imposing an accurate live-dead evaluation and producing a true LC50 value. Guidelines imposed using such data will facilitate compliance and provide an accurate value for an LC50.

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