Ultrastructure of NPC/HK1 cells heterotransplanted in athymic nude mice

1982 ◽  
Vol 40 ◽  
pp. 236-237
Author(s):  
E.C. Chew ◽  
C.L. Li ◽  
D.P. Huang ◽  
H.C. Ho ◽  
L.S. Mak ◽  
...  
Keyword(s):  
Cell Line ◽  
Nude Mice ◽  
Biopsy Specimen ◽  
Epithelial Cell Line ◽  
Present Communication ◽  
Recurrent Tumour ◽  
Athymic Nude Mice ◽  
Cell Line Establishment ◽  
Fine Structures ◽  
Well Differentiated

An epithelial cell line, NPC/HK1, has recently been established from a biopsy specimen of a recurrent tumour of the nasopharynx which was histologically diagnosed as a moderately to well differentiated squamous cell carcinoma. A definite decrease in the amount of tonofilaments and desmosomes in the NPC/HK1 cells during the cell line establishment was observed. The present communication reports on the fine structures of the NPC/HK1 cells heterotraneplanted in athymic nude mice.

An Established Epithelial Cell Line (NPC/HK1) from a Nasopharyngeal Carcinoma - II. A Sem Study

1982 ◽  
Vol 40 ◽  
pp. 224-225
Author(s):  
Li C.L. ◽  
Chew E.C. ◽  
Huang D.P. ◽  
Ho H.C. ◽  
Mak L.S. ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Epithelial Cell ◽  
Surface Morphology ◽  
Cell Line ◽  
Epithelial Cell Line ◽  
Present Communication ◽  
Cells In Culture ◽  
Sem Study ◽  
Well Differentiated

An epithelial cell line, NPC/HK1, has recently been successfully established from a nasopharyngeal carcinoma of the moderately to well differentiated squamous type. The present communication reports on the surface morphology of the NPC/HK1 cells in culture.

An Established Epithelial Cell Line (NPC/HK1) from a Nasopharyngeal Carcinoma - I. A Tem Study

1982 ◽  
Vol 40 ◽  
pp. 222-223
Author(s):  
C.L. Li ◽  
E.C. Chew ◽  
D.P. Huang ◽  
H.C. Ho ◽  
M. Lui ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Epithelial Cell ◽  
Cell Line ◽  
Squamous Carcinoma ◽  
Carcinoma Cells ◽  
Epithelial Cell Line ◽  
Original Tumour ◽  
Tumour Biopsy ◽  
Fine Structures ◽  
Well Differentiated

An epithelial cell line, NPC/HK1, has recently been successfully established from a moderately to well differentiated squamous carcinoma of the nasopharynx. This communication reports on the fine structures observed in the carcinoma cells of the original tumour biopsy and those of the NPC/HK1 cells from different culture generations derived from it.

scholarly journals Production of multiple cytokines and induction of cachexia in athymic nude mice by a new anaplastic thyroid carcinoma cell line

2003 ◽  
Vol 179 (3) ◽  
pp. 387-394 ◽  
Author(s):  
JW Chang ◽  
KY Yeh ◽  
YC Shen ◽  
JJ Hsieh ◽  
CK Chuang ◽  
...  
Keyword(s):  
Thyroid Cancer ◽  
Growth Factor ◽  
Cell Line ◽  
Cancer Cell ◽  
Nude Mice ◽  
Cancer Cell Line ◽  
Anaplastic Thyroid Cancer ◽  
Thyroid Cancer Cell ◽  
Athymic Nude Mice ◽  
Thyroid Cancer Cell Line

An anaplastic thyroid cancer cell line, Thena, was recently established in our laboratory following radical thyroidectomy of a patient with anaplastic thyroid cancer. Microscopically, Thena cells were spindle-shaped or small round cells. Thena cells were reactive with cytokeratin AE1/AE3 antibodies, epithelial membrane antigen, interleukin (IL)-6, epithelial growth factor receptor, transforming growth factor (TGF)-alpha, vascular endothelial growth factor, and vimentin. Thena cells secreted high levels of IL-6, leukemia inhibitor factor (LIF), tumor necrosis factor (TNF)-alpha, and TGF-beta1 in the culture supernatants, as determined by enzyme-linked immunosorbent assay. When subcutaneously injected with Thena cells, athymic nude mice developed tumor masses in the skin within 2 weeks. Furthermore, Thena cells induced cachexia in these tumor-bearing mice. High levels of human IL-6, LIF and TGF-beta1 were detected in the mouse sera. To our knowledge, the Thena cell line is the first thyroid cancer cell line reported to induce cachexia in nude mice. This cachectic animal model is worthy of further study to explore the treatment of thyroid cancer-induced cachexia.

New acceptor cell for transfected genomic DNA: oncogene transfer into a mouse mammary epithelial cell line

1985 ◽  
Vol 5 (1) ◽  
pp. 268-272
Author(s):  
N E Hynes ◽  
R Jaggi ◽  
S C Kozma ◽  
R Ball ◽  
D Muellener ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Cell Line ◽  
Nude Mice ◽  
Genomic Dna ◽  
Mammary Epithelial Cells ◽  
Human Tumor ◽  
Mammary Epithelial ◽  
Epithelial Cell Line ◽  
Exogenous Dna ◽  
Stable Transfectants

A line of mouse mammary epithelial cells (NMuMG) has been characterized for its ability to be stably transfected with exogenous DNA. A transfection frequency of at least 1 cell per 1,000 was obtained with the pSV2neo plasmid. Several thousand G418-resistant NMuMG cell clones can easily be generated in cotransfection of genomic DNA and pSV2neo. The NMuMG cells were isolated from normal mammary glands and do not form malignant lesions when injected into nude mice. We have cotransfected NMuMG cells with pSV2neo and genomic DNA from the human EJ bladder carcinoma line, a cell line which contains an activated c-rasH oncogene. When a pool of 4,700 G418-resistant colonies was injected into nude mice, tumors were obtained. These tumors contain a transfected human rasH gene. Genomic DNA transfection into a line of mouse epithelial cells, in combination with the selection of stable transfectants and tumor induction in nude mice, can be used to screen human tumor DNA for the presence of activated oncogenes.

scholarly journals New acceptor cell for transfected genomic DNA: oncogene transfer into a mouse mammary epithelial cell line.

1985 ◽  
Vol 5 (1) ◽  
pp. 268-272 ◽  
Author(s):  
N E Hynes ◽  
R Jaggi ◽  
S C Kozma ◽  
R Ball ◽  
D Muellener ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Cell Line ◽  
Nude Mice ◽  
Genomic Dna ◽  
Mammary Epithelial Cells ◽  
Human Tumor ◽  
Mammary Epithelial ◽  
Epithelial Cell Line ◽  
Exogenous Dna ◽  
Stable Transfectants

A line of mouse mammary epithelial cells (NMuMG) has been characterized for its ability to be stably transfected with exogenous DNA. A transfection frequency of at least 1 cell per 1,000 was obtained with the pSV2neo plasmid. Several thousand G418-resistant NMuMG cell clones can easily be generated in cotransfection of genomic DNA and pSV2neo. The NMuMG cells were isolated from normal mammary glands and do not form malignant lesions when injected into nude mice. We have cotransfected NMuMG cells with pSV2neo and genomic DNA from the human EJ bladder carcinoma line, a cell line which contains an activated c-rasH oncogene. When a pool of 4,700 G418-resistant colonies was injected into nude mice, tumors were obtained. These tumors contain a transfected human rasH gene. Genomic DNA transfection into a line of mouse epithelial cells, in combination with the selection of stable transfectants and tumor induction in nude mice, can be used to screen human tumor DNA for the presence of activated oncogenes.

The Effect of Radiation Therapy and Hyperthermia on a Human Prostatic Carcinoma Cell Line Grown in Athymic Nude Mice

1991 ◽  
Vol 145 (3) ◽  
pp. 654-656 ◽  
Author(s):  
Issac Kaver ◽  
Warren W. Koontz ◽  
John D. Wilson ◽  
John M. Guice ◽  
Joy L. Ware
Keyword(s):  
Radiation Therapy ◽  
Cell Line ◽  
Nude Mice ◽  
Prostatic Carcinoma ◽  
Carcinoma Cell ◽  
Carcinoma Cell Line ◽  
Athymic Nude Mice

scholarly journals T-cell lymphoma induction by radiation leukemia virus in athymic nude mice.

1978 ◽  
Vol 148 (5) ◽  
pp. 1292-1310 ◽  
Author(s):  
P Ricciardi-Castagnoli ◽  
M Lieberman ◽  
O Finn ◽  
H S Kaplan
Keyword(s):  
T Cell ◽  
Cell Line ◽  
Nude Mice ◽  
Leukemia Virus ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Target Cells ◽  
Transferase Activity ◽  
Athymic Nude Mice ◽  
Cell Markers

We report the development of extrathymic lymphoblastic lymphomas in RadLV-inoculated congenitally athymic nude mice. Thus, a leukemogenic virus which appears to require the presence of a thymus for its replication in normothymic mice can infect and transform target cells in the absence of this organ in the athymic host. The cells of one of these lymphomas have been established in vitro as a permanent cell line, BALB/Nu1. This cell line as well as a lymphoma induced in NIH/Swiss nude mice exhibit several T-cell markers, including terminal deoxynucleotidyl transferase activity, Thy-1.2, and Ly-2.2, but not Ly-1.2 nor TL. Ig determinants were not detected. The characteristics of the tumor cells support the view that cells with T-cell markers may normally exist in nude mice and undergo neoplastic transformation and clonal expansion after infection with a leukemogenic virus. The alternative possibility that virus-induced differentiation of prothymocytes may lead to the expression of Thy-1.2 and Ly-2.2 antigens is also considered. BALB/Nu1 cells release large numbers of type C viral particles. The virus, designated radiation leukemia virus (RadLV)/Nu1, has RTase activity and the protein profile characteristic of murine leukemia virus (MuLV). In radioimmunoassays, it cross-reacts completely with RadLV/VL3, a virus obtained from RadLV-induced C57BL/Ka thymic lymphoma cells in culture, and slightly with a xenotropic virus (BALB:virus-2) and with AKR MuLV. On inoculation into C57BL/Ka mice it has thymotropic and leukemogenic activity. In vitro it is B-tropic, poorly fibrotropic, and has limited xenotropic activity. Thus, RadLV/Nu1 appears to be biologically and serologically similar or identical to its parent virus, RadLV.

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