Origin of Hepatic Nuclear Inclusion Bodies

1973 ◽  
Vol 31 ◽  
pp. 426-427
Author(s):  
F. G. Zaki ◽  
J. A. Greenlee ◽  
C. H. Keysser
Keyword(s):  
Inclusion Bodies ◽  
Storage Disease ◽  
Glycogen Storage ◽  
Nutritional Deficiencies ◽  
Nuclear Inclusion ◽  
Electron Microscopic ◽  
Human Liver Cells ◽  
Microscopic Studies ◽  
Per Se ◽  
Experimental Liver

Nuclear inclusion bodies seen in human liver cells may appear in light microscopy as deposits of fat or glycogen resulting from various diseases such as diabetes, hepatitis, cholestasis or glycogen storage disease. These deposits have been also encountered in experimental liver injury and in our animals subjected to nutritional deficiencies, drug intoxication and hepatocarcinogens. Sometimes these deposits fail to demonstrate the presence of fat or glycogen and show PAS negative reaction. Such deposits are considered as viral products.Electron microscopic studies of these nuclei revealed that such inclusion bodies were not products of the nucleus per se but were mere segments of endoplasmic reticulum trapped inside invaginating nuclei (Fig. 1-3).

The Ultrastructure of Crystalliferous Cells in Some Lecythidaceae With a Discussion of Their Terminology

IAWA Journal ◽  
1984 ◽  
Vol 5 (3) ◽  
pp. 229-236 ◽  
Author(s):  
N. Parameswaran ◽  
H.-G. Richter
Keyword(s):  
Electron Microscopic ◽  
Axial Parenchyma ◽  
Individual Crystal ◽  
Microscopic Studies ◽  
Per Se ◽  
The Individual

On the basis of a light microscopic c1assification of the genera of the Lecythidaceae according to the presence of crystalliferous cells in the axial wood parenchyma an attempt was made to characterise these cells at the fine structurallevel. Electron microscopic studies of the genera Allantoma, Grias and Gustavia revealed normal cross walls, as well as septumIike walls separating the individual crystal-containing units in the axial parenchyma strand. Based on these findings the terminology of the crystalliferous cells per se is discussed at some length.

Epinephrine-induced biochemical and electron microscopic alterations in generalized glycogen storage disease

1965 ◽  
Vol 67 (5) ◽  
pp. 977
Author(s):  
George Hug ◽  
John C. Garancis ◽  
William K. Schubert ◽  
Samuel Kaplan
Keyword(s):  
Glycogen Storage Disease ◽  
Storage Disease ◽  
Glycogen Storage ◽  
Electron Microscopic

scholarly journals Lysosomal Storage Disease in an Emu (Dromaius novaehollandiae)

1996 ◽  
Vol 33 (3) ◽  
pp. 365-366 ◽  
Author(s):  
D. Y. Rim ◽  
D. Y. Cho ◽  
H. W. Taylor
Keyword(s):  
Spinal Cord ◽  
Lysosomal Storage Disease ◽  
Storage Disease ◽  
Histochemical Staining ◽  
Lysosomal Storage ◽  
Electron Microscopic ◽  
First Case ◽  
Dromaius Novaehollandiae ◽  
Microscopic Studies ◽  
The Brain

Lysosomal storage disease involving the brain, spinal cord, liver, and spleen was discovered in a 6-month-old male emu ( Dromaius novaehollandiae). The diagnosis was based on light and electron microscopic studies and histochemical staining characteristics. This is the first case of lysosomal storage disease reported in a ratite.

scholarly journals Cytoplasmic Inclusions in Acute Hemocytoblastic Leukemia: Case Report

Blood ◽  
1961 ◽  
Vol 17 (3) ◽  
pp. 334-344 ◽  
Author(s):  
L. G. SARAIVA ◽  
G. P. BOURROUL ◽  
M. SILVEIRA ◽  
J. D. PROSPERO ◽  
J. J. ANGULO
Keyword(s):  
Electron Microscopy ◽  
Bone Marrow ◽  
Case Report ◽  
Inclusion Bodies ◽  
Desoxyribonucleic Acid ◽  
Electron Microscopic ◽  
Cytoplasmic Inclusions ◽  
Negative Results ◽  
Microscopic Studies ◽  
Viral Nature

Abstract A case of hemocytoblastic leukemia in a 56 year old white man is reported which was characterized by cytoplasmic inclusions in some of the hemocytoblasts, reticulum cells and macrophages of the bone marrow as well as in some of the hemocytoblasts of the circulating blood. Phase and electron microscopic studies confirmed the corpuscular nature of the inclusions. Cytochemical stains gave negative results when tested for the presence of mucoproteins, lipids, phospholipids. neutral fat, desoxyribonucleic acid, peroxidase and phosphatases. The test for polysaccharides was slightly positive. Preliminary studies underway with electron microscopy suggest the possibility of a viral nature of the inclusion bodies.

Electron microscopic studies on ultrathin frozen sections of lactating mouse mammary gland

1978 ◽  
Vol 36 (2) ◽  
pp. 46-47
Author(s):  
Jan Zarzycki ◽  
Joseph Szroeder
Keyword(s):  
Mammary Gland ◽  
Protein Denaturation ◽  
Chemical Constituents ◽  
Freeze Substitution ◽  
Electron Microscopic Examination ◽  
Mouse Mammary Gland ◽  
Electron Microscopic ◽  
Preparation Methods ◽  
Microscopic Studies ◽  
Ultrathin Frozen Sections

The mammary gland ultrastructure in various functional states is the object of our investigations. The material prepared for electron microscopic examination by the conventional chemical methods has several limitations, the most important are the protein denaturation processes and the loss of large amounts of chemical constituents from the cells. In relevance to this,one can't be sure about a degree the observed images are adequate to the realy ultrastructure of a living cell. To avoid the disadvantages of the chemical preparation methods,some autors worked out alternative physical methods based on tissue freezing / freeze-drying, freeze-substitution, freeze-eatching techniqs/; actually the technique of cryoultraraicrotomy,i,e.cutting ultrathin sections from deep frozen specimens is assented as a complete alternative method. According to the limitations of the routine plastic embbeding methods we were interested to analize the mammary gland ultrastructure during lactation by the cryoultramicrotomy method.

Early Development of Drug-Induced Hepatic Necrosis

1971 ◽  
Vol 29 ◽  
pp. 576-577
Author(s):  
F. G. Zaki ◽  
E. Detzi ◽  
C. H. Keysser
Keyword(s):  
Early Development ◽  
Hepatic Necrosis ◽  
Screening Tests ◽  
Dense Matrix ◽  
Sprague Dawley ◽  
Electron Microscopic ◽  
Drug Induced ◽  
Hepatocellular Damage ◽  
Sprague Dawley Rats ◽  
Microscopic Studies

This study represents the first in a series of investigations carried out to elucidate the mechanism(s) of early hepatocellular damage induced by drugs and other related compounds. During screening tests of CNS-active compounds in rats, it has been found that daily oral administration of one of these compounds at a dose level of 40 mg. per kg. of body weight induced diffuse massive hepatic necrosis within 7 weeks in Charles River Sprague Dawley rats of both sexes. Partial hepatectomy enhanced the development of this peculiar type of necrosis (3 weeks instead of 7) while treatment with phenobarbital prior to the administration of the drug delayed the appearance of necrosis but did not reduce its severity.Electron microscopic studies revealed that early development of this liver injury (2 days after the administration of the drug) appeared in the form of small dark osmiophilic vesicles located around the bile canaliculi of all hepatocytes (Fig. 1). These structures differed from the regular microbodies or the pericanalicular multivesicular bodies. They first appeared regularly rounded with electron dense matrix bound with a single membrane. After one week on the drug, these vesicles appeared vacuolated and resembled autophagosomes which soon developed whorls of concentric lamellae or cisterns characteristic of lysosomes (Fig. 2). These lysosomes were found, later on, scattered all over the hepatocytes.

Electron Microscopic identification of Pre-B Lymphocytes in the Bone Marrow of a Patient with Chronic Myelocytic Leukemic in Blast Crisis

1982 ◽  
Vol 40 ◽  
pp. 346-347
Author(s):  
T. Mullin ◽  
G. Yee ◽  
M. Aheam ◽  
J. Trujillo
Keyword(s):  
Blast Crisis ◽  
Immunoglobulin M ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Transformation Theory ◽  
Electron Microscopic ◽  
Chemotherapeutic Sensitivity ◽  
Microscopic Studies ◽  
Hematopoietic Precursor ◽  
Lymphoblastic Transformation ◽  
B Lineage

There have been numerous reports in the current literature suggesting that hematopoietic precursor cells in some human chronic myelocytic leukemias (CML) undergo lymphoblastic transformation at the time of the acute blast crisis (BC) stage. The primary evidence offered in support of this transformation theory--lymphoblastic appearing morphology, increased terminal deoxynucleotidyl transferase (TdT) activity, and chemotherapeutic sensitivity to vincristine and prednisone--has been indirect, however, since these features may occur in nonlymphoid cells. More direct support for the Pre-B lineage of these cells has recently been provided by immunofluorescent light microscopic studies demonstrating the presence of intracytoplasmic immunoglobulin M (IgM) in these CML-BC cells.

Novel methods for demonstrating osseointegration of hydroxylapatite-coated titanium hip prostheses

1991 ◽  
Vol 49 ◽  
pp. 34-35 ◽  
Author(s):  
P. Frayssinet ◽  
J. Hanker ◽  
D. Hardy ◽  
B. Giammara
Keyword(s):  
Hip Prosthesis ◽  
Ethanol Concentration ◽  
Bone Matrix ◽  
Electron Microscopic Examination ◽  
Electron Microscopic ◽  
Hip Prostheses ◽  
Cellular Inclusions ◽  
Microscopic Studies ◽  
Diamond Saw ◽  
Plasma Sprayed

Prostheses implanted in hard tissues cannot be processed for electron microscopic examination or microanalysis in the same way as those in other tissues. For these reasons, we have developed methods allowing light and electron microscopic studies as well as microanalysis of the interface between bone and a metal biomaterial coated by plasma-sprayed hydroxylapatite(HA) ceramic.An HA-coated titanium hip prosthesis (Corail, Landos, France), which had been implanted for two years, was removed after death (unrelated to the orthopaedic problem). After fixation it was dehydrated in solutions of increasing ethanol concentration prior to embedment in polymethylmethacrylate(PMMA). Transverse femur sections were obtained with a diamond saw and the sections then carefully ground to a thickness of 200 microns. Plastic-embedded sections were stained for calcium with a silver methenamine modification of the von Kossa method for calcium staining and coated by carbon. They have been examined by back-scatter SEM on an ISI-SS60 operated at 25 KV. EDAX has been done on cellular inclusions and extracellular bone matrix.

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