The Ultrastructure of Crystalliferous Cells in Some Lecythidaceae With a Discussion of Their Terminology

IAWA Journal ◽  
1984 ◽  
Vol 5 (3) ◽  
pp. 229-236 ◽  
Author(s):  
N. Parameswaran ◽  
H.-G. Richter
Keyword(s):  
Electron Microscopic ◽  
Axial Parenchyma ◽  
Individual Crystal ◽  
Microscopic Studies ◽  
Per Se ◽  
The Individual

On the basis of a light microscopic c1assification of the genera of the Lecythidaceae according to the presence of crystalliferous cells in the axial wood parenchyma an attempt was made to characterise these cells at the fine structurallevel. Electron microscopic studies of the genera Allantoma, Grias and Gustavia revealed normal cross walls, as well as septumIike walls separating the individual crystal-containing units in the axial parenchyma strand. Based on these findings the terminology of the crystalliferous cells per se is discussed at some length.

Origin of Hepatic Nuclear Inclusion Bodies

1973 ◽  
Vol 31 ◽  
pp. 426-427
Author(s):  
F. G. Zaki ◽  
J. A. Greenlee ◽  
C. H. Keysser
Keyword(s):  
Inclusion Bodies ◽  
Storage Disease ◽  
Glycogen Storage ◽  
Nutritional Deficiencies ◽  
Nuclear Inclusion ◽  
Electron Microscopic ◽  
Human Liver Cells ◽  
Microscopic Studies ◽  
Per Se ◽  
Experimental Liver

Nuclear inclusion bodies seen in human liver cells may appear in light microscopy as deposits of fat or glycogen resulting from various diseases such as diabetes, hepatitis, cholestasis or glycogen storage disease. These deposits have been also encountered in experimental liver injury and in our animals subjected to nutritional deficiencies, drug intoxication and hepatocarcinogens. Sometimes these deposits fail to demonstrate the presence of fat or glycogen and show PAS negative reaction. Such deposits are considered as viral products.Electron microscopic studies of these nuclei revealed that such inclusion bodies were not products of the nucleus per se but were mere segments of endoplasmic reticulum trapped inside invaginating nuclei (Fig. 1-3).

scholarly journals Immuno-electron-microscopic studies on the subcellular distribution of rat liver epoxide hydrolase and the effect of phenobarbitone and 2-acetamidofluorene treatment

1982 ◽  
Vol 202 (3) ◽  
pp. 677-686 ◽  
Author(s):  
F Waechter ◽  
P Bentley ◽  
M Germann ◽  
F Oesch ◽  
W Stäubli
Keyword(s):  
Electron Microscopy ◽  
Rat Liver ◽  
Subcellular Distribution ◽  
Epoxide Hydrolase ◽  
Specific Binding ◽  
Subcellular Fractions ◽  
Electron Microscopic ◽  
Immuno Electron Microscopy ◽  
Microscopic Studies ◽  
The Individual

The distribution of rat liver epoxide hydrolase in various subcellular fractions was investigated by immuno-electron-microscopy. Ferritin-linked monospecific anti-(epoxide hydrolase) immunoglobulins bound specifically to the cytoplasmic surfaces of total microsomal preparations and smooth and rough microsomal fractions as well as the nuclear envelope. Specific binding was not observed when the ferritin conjugates were incubated with peroxisomes, lysosomes and mitochondria. The average specific ferritin load of the individual subcellular fractions correlated well with the measured epoxide hydrolase activities. This correlation was observed with fractions prepared from control, phenobarbitone-treated and 2-acetamidofluorene-treated rats.

Effect of formaldehyde inhalation on rat livers: A light and electron microscopic study

2010 ◽  
Vol 26 (2) ◽  
pp. 113-119 ◽  
Author(s):  
Selman Cikmaz ◽  
Tunc Kutoglu ◽  
Mehmet Kanter ◽  
Recep Mesut
Keyword(s):  
Liver Tissue ◽  
Exposure Period ◽  
Electron Microscopic Level ◽  
Albino Rats ◽  
Electron Microscopic ◽  
Tissue Samples ◽  
Microscopic Evaluation ◽  
Microscopic Studies ◽  
Wistar Albino Rats ◽  
The Individual

It is well known that formaldehyde (FA) is cytotoxic and potentially carcinogenic. Although the individual effects of this reactant on cells has been investigated, the cytotoxicity exerted by the coexistence of FA is poorly understood. The aim of this study was to investigate the effects of FA on the liver in rats, by light and electron microscopic level. We used 18 Wistar albino rats divided into three groups, exposed to 0 (control), 19.7 ppm FA gas for a total of 4 weeks, 8 h/day, 5 days a week (subacute) and 20.3 parts per million (ppm) FA gas for a total of 13 weeks, 8 h/day, 5 days a week (subchronic). After the completion of the exposure period, they were sacrificed by decapitation and their liver tissue samples were taken in order to be processed for light and electron microscopic studies. Light microscopic evaluation of liver tissue samples of FA-exposed rats revealed enlarged sinusoids filled with blood and mononuclear cell infiltration in the portal areas and around the central veins. In addition, some of the hepatocytes showed loss of cytoplasm, and some had a hyperchromatic nucleus. The cells of FA-exposed livers, on the other hand, showed an electron-lucent ground-cytoplasm and a hypertrophy of the smooth-surfaced endoplasmic reticulum. In conclusion, we observed that exposure FA caused diverse histopathological changes indicating the destruction in the liver tissue and this destruction has direct relationship with the length of the exposure period.

scholarly journals ELECTRON MICROSCOPIC STUDIES ON THE INDIRECT FLIGHT MUSCLES OFDROSOPHILAMELANOGASTER

1963 ◽  
Vol 17 (2) ◽  
pp. 363-0373 ◽  
Author(s):  
S. Ahmad Shafiq
Keyword(s):  
Cell Membranes ◽  
Plasma Membranes ◽  
Electron Microscopic ◽  
Cytoplasmic Inclusions ◽  
Fibrillar Material ◽  
Microscopic Studies ◽  
Flight Muscles ◽  
The Individual

The differentiation of the indirect flight muscles was studied in the various pupal stages of Drosophila. Fibrillar material originates in the young basophilic myoblasts in the form of short myofilamants distributed irregularly near the cell membranes. The filaments later become grouped into bundles (fibrils). Certain "Z bodies" appear to be important during this process. The "Z bodies" may possibly be centriolar derivatives and are the precursors of the Z bands. The first formed fibrils (having about 30 thick myofilaments) are already divided into sarcomeres by Z bands. These sarcomeres, however, seem to be shorter than those of the adult fibrils.The H band differentiates in fibrils having about 40 thick myofilaments; the fibrils constrict in the middle of each sarcomere during this process. The individual myofibrils increase from about 0.3 µ to 1.5 µ in diameter during development, apparently by addition of new filaments on the periphery of the fibrils. The ribosomes seem to be the only cytoplasmic inclusions which are closely associated with these growing myofibrils. Disintegration of the plasma membranes limiting individual myoblasts was commonly seen during development of flight muscles, supporting the view that the multinuclear condition of the fibers of these muscles is due to fusion of myoblasts.

Effect of Nickel and Aluminium on Solid State Transformations in SG Iron

1984 ◽  
Vol 34 ◽  
Author(s):  
A. C. Ganguli ◽  
A. K. Chakrabarti ◽  
S. C. Dasgupta
Keyword(s):  
Solid State ◽  
Solution Treatment ◽  
X Ray Diffraction ◽  
Electron Microscopic ◽  
X Ray ◽  
Temperature Range ◽  
Microscopic Studies ◽  
Sg Iron ◽  
The Individual ◽  
Kinetics Of

ABSTRACTThe individual and combined addition of nickel and aluminium to S.g. iron influences several solid state transformations. Nickel depresses the temperature range for reversion of ferrite and pearlite to austenite on heating, while aluminium raises it. Combined addition of nickel and aluminium restores the temperature range closer to that for unalloyed iron. But nickel enhances the kinetics of ferritisation during tempering and weakly retards recrystallisation of ferrite grains. Aluminium strongly retards both the ferritisation and recrystallisation processes. Ferritisation kinetics is enhanced and recrystallisation of ferrite grains is also hastened in alloys containing nickel and aluminium. Alloys containing nickel and aluminium also respond to agehardening treatment in the temperature range 450–550°C after a solution treatment-cum-ferritising anneal at 700°C. On the basis of X-ray diffraction and electron microscopic studies, a probable mechanism of ageing has been proposed.

Electron microscopic studies on ultrathin frozen sections of lactating mouse mammary gland

1978 ◽  
Vol 36 (2) ◽  
pp. 46-47
Author(s):  
Jan Zarzycki ◽  
Joseph Szroeder
Keyword(s):  
Mammary Gland ◽  
Protein Denaturation ◽  
Chemical Constituents ◽  
Freeze Substitution ◽  
Electron Microscopic Examination ◽  
Mouse Mammary Gland ◽  
Electron Microscopic ◽  
Preparation Methods ◽  
Microscopic Studies ◽  
Ultrathin Frozen Sections

The mammary gland ultrastructure in various functional states is the object of our investigations. The material prepared for electron microscopic examination by the conventional chemical methods has several limitations, the most important are the protein denaturation processes and the loss of large amounts of chemical constituents from the cells. In relevance to this,one can't be sure about a degree the observed images are adequate to the realy ultrastructure of a living cell. To avoid the disadvantages of the chemical preparation methods,some autors worked out alternative physical methods based on tissue freezing / freeze-drying, freeze-substitution, freeze-eatching techniqs/; actually the technique of cryoultraraicrotomy,i,e.cutting ultrathin sections from deep frozen specimens is assented as a complete alternative method. According to the limitations of the routine plastic embbeding methods we were interested to analize the mammary gland ultrastructure during lactation by the cryoultramicrotomy method.

Early Development of Drug-Induced Hepatic Necrosis

1971 ◽  
Vol 29 ◽  
pp. 576-577
Author(s):  
F. G. Zaki ◽  
E. Detzi ◽  
C. H. Keysser
Keyword(s):  
Early Development ◽  
Hepatic Necrosis ◽  
Screening Tests ◽  
Dense Matrix ◽  
Sprague Dawley ◽  
Electron Microscopic ◽  
Drug Induced ◽  
Hepatocellular Damage ◽  
Sprague Dawley Rats ◽  
Microscopic Studies

This study represents the first in a series of investigations carried out to elucidate the mechanism(s) of early hepatocellular damage induced by drugs and other related compounds. During screening tests of CNS-active compounds in rats, it has been found that daily oral administration of one of these compounds at a dose level of 40 mg. per kg. of body weight induced diffuse massive hepatic necrosis within 7 weeks in Charles River Sprague Dawley rats of both sexes. Partial hepatectomy enhanced the development of this peculiar type of necrosis (3 weeks instead of 7) while treatment with phenobarbital prior to the administration of the drug delayed the appearance of necrosis but did not reduce its severity.Electron microscopic studies revealed that early development of this liver injury (2 days after the administration of the drug) appeared in the form of small dark osmiophilic vesicles located around the bile canaliculi of all hepatocytes (Fig. 1). These structures differed from the regular microbodies or the pericanalicular multivesicular bodies. They first appeared regularly rounded with electron dense matrix bound with a single membrane. After one week on the drug, these vesicles appeared vacuolated and resembled autophagosomes which soon developed whorls of concentric lamellae or cisterns characteristic of lysosomes (Fig. 2). These lysosomes were found, later on, scattered all over the hepatocytes.

Electron Microscopic identification of Pre-B Lymphocytes in the Bone Marrow of a Patient with Chronic Myelocytic Leukemic in Blast Crisis

1982 ◽  
Vol 40 ◽  
pp. 346-347
Author(s):  
T. Mullin ◽  
G. Yee ◽  
M. Aheam ◽  
J. Trujillo
Keyword(s):  
Blast Crisis ◽  
Immunoglobulin M ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Transformation Theory ◽  
Electron Microscopic ◽  
Chemotherapeutic Sensitivity ◽  
Microscopic Studies ◽  
Hematopoietic Precursor ◽  
Lymphoblastic Transformation ◽  
B Lineage

There have been numerous reports in the current literature suggesting that hematopoietic precursor cells in some human chronic myelocytic leukemias (CML) undergo lymphoblastic transformation at the time of the acute blast crisis (BC) stage. The primary evidence offered in support of this transformation theory--lymphoblastic appearing morphology, increased terminal deoxynucleotidyl transferase (TdT) activity, and chemotherapeutic sensitivity to vincristine and prednisone--has been indirect, however, since these features may occur in nonlymphoid cells. More direct support for the Pre-B lineage of these cells has recently been provided by immunofluorescent light microscopic studies demonstrating the presence of intracytoplasmic immunoglobulin M (IgM) in these CML-BC cells.

Novel methods for demonstrating osseointegration of hydroxylapatite-coated titanium hip prostheses

1991 ◽  
Vol 49 ◽  
pp. 34-35 ◽  
Author(s):  
P. Frayssinet ◽  
J. Hanker ◽  
D. Hardy ◽  
B. Giammara
Keyword(s):  
Hip Prosthesis ◽  
Ethanol Concentration ◽  
Bone Matrix ◽  
Electron Microscopic Examination ◽  
Electron Microscopic ◽  
Hip Prostheses ◽  
Cellular Inclusions ◽  
Microscopic Studies ◽  
Diamond Saw ◽  
Plasma Sprayed

Prostheses implanted in hard tissues cannot be processed for electron microscopic examination or microanalysis in the same way as those in other tissues. For these reasons, we have developed methods allowing light and electron microscopic studies as well as microanalysis of the interface between bone and a metal biomaterial coated by plasma-sprayed hydroxylapatite(HA) ceramic.An HA-coated titanium hip prosthesis (Corail, Landos, France), which had been implanted for two years, was removed after death (unrelated to the orthopaedic problem). After fixation it was dehydrated in solutions of increasing ethanol concentration prior to embedment in polymethylmethacrylate(PMMA). Transverse femur sections were obtained with a diamond saw and the sections then carefully ground to a thickness of 200 microns. Plastic-embedded sections were stained for calcium with a silver methenamine modification of the von Kossa method for calcium staining and coated by carbon. They have been examined by back-scatter SEM on an ISI-SS60 operated at 25 KV. EDAX has been done on cellular inclusions and extracellular bone matrix.

Dendritic cells of the human epidermis: immuno-electron microscopic studies of la antigen reactivity

1978 ◽  
Vol 36 (2) ◽  
pp. 644-645
Author(s):  
G. Rowden ◽  
M. G. Lewis ◽  
T. M. Phillips
Keyword(s):  
Dendritic Cells ◽  
Langerhans Cells ◽  
Cell Types ◽  
Cell Type ◽  
Electron Microscopic ◽  
La Antigen ◽  
Microscopic Studies ◽  
A Cell ◽  
C3 Receptors ◽  
Antigen Reactivity

Langerhans cells of mammalian stratified squamous epithelial have proven to be an enigma since their discovery in 1868. These dendritic suprabasal cells have been considered as related to melanocytes either as effete cells, or as post divisional products. Although grafting experiments seemed to demonstrate the independence of the cell types, much confusion still exists. The presence in the epidermis of a cell type with morphological features seemingly shared by melanocytes and Langerhans cells has been especially troublesome. This so called "indeterminate", or " -dendritic cell" lacks both Langerhans cells granules and melanosomes, yet it is clearly not a keratinocyte. Suggestions have been made that it is related to either Langerhans cells or melanocyte. Recent studies have unequivocally demonstrated that Langerhans cells are independent cells with immune function. They display Fc and C3 receptors on their surface as well as la (immune region associated) antigens.

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