Scanning and Transmission Electron Microscopy of Endomentrium in Women Wearing TCu-380-A IUD 's

1977 ◽  
Vol 35 ◽  
pp. 666-667
Author(s):  
A. Gonzalez Angulo ◽  
R. Berlioz ◽  
R. Aznar
Keyword(s):  
Copper Wire ◽  
Uterine Cavity ◽  
Morphological Evidence ◽  
Secretory Phase ◽  
Ultrastructural Studies ◽  
Proliferative Phase ◽  
Contraceptive Devices ◽  
Transmission Electron ◽  
Intrauterine Contraceptive ◽  
Myelin Figure

Recent ultrastructural studies on endometrial tissues from women wearing copper, wire intrauterine devices have disclosed morphological evidence of impaired glycogen degradation and secretion resulting in interference with the viability of blastocysts. Reduced microapocrine secretion observed with the scanning electron microscope supports this (1). In addition, organelle modifications have been observed in the epithelial cells of these women. The changes are seen in biopsies taken in the proliferative phase of the cycle and consist of mitochondrial vacuolation and myelin figure formation. These modifications disappear in the secretory phase and therefore have been regarded as reversible (2).The aim of the present studies was to investigate surface epithelial changes as well as organelle modifications in relation to the site of contact with an IUD that releases greater amounts of copper. Endometrial tissue was obtained from the uterine cavity of four young women wearing TCu-380-A intrauterine contraceptive devices for 4-6 weeks.

scholarly journals WOMEN IN REPRODUCTIVE SCIENCE: My WOMBan’s life: understanding human endometrial function

Reproduction ◽  
2019 ◽  
Vol 158 (6) ◽  
pp. F55-F67 ◽  
Author(s):  
Lois A Salamonsen
Keyword(s):  
Uterine Cavity ◽  
Abnormal Uterine Bleeding ◽  
Endometrial Receptivity ◽  
Uterine Bleeding ◽  
Menstrual Disorders ◽  
Secretory Phase ◽  
Infertile Women ◽  
Proliferative Phase ◽  
Cellular Changes ◽  
Reproductive Science

The focus of my life in science has been to improve reproductive health for women, with an emphasis on the endometrium, the most dynamic tissue in the human body: its remarkable cyclical remodelling is essential for the establishment of pregnancy. The most notable events in a woman’s endometrial cycle are menstruation and endometrial repair, regeneration of the endometrium during the proliferative phase, attainment of receptivity by the mid-secretory phase of the cycle and the embryo–maternal interactions that initiate peri-implantation events within the microenvironment of the uterine cavity. I have contributed to understanding the molecular and cellular changes underpinning these events, and how disturbance of them leads to menstrual disorders, infertility and endometrial diseases including abnormal uterine bleeding, endometriosis and endometrial cancer. My team has contributed to changes in clinical IVF practice, to a new diagnostic for endometrial receptivity in infertile women and to enhancing endometrial repair. I have shared my world with many amazing younger scientists: it has indeed been a privileged journey.

Calcification of Intrauterine Contraceptive Devices (IUDs)

1990 ◽  
Vol 48 (3) ◽  
pp. 212-213
Author(s):  
Saeed R. Khan
Keyword(s):  
Electron Microscopy ◽  
Prolonged Exposure ◽  
X Ray Diffraction ◽  
X Ray ◽  
Contraceptive Devices ◽  
Inorganic Substances ◽  
Transmission Electron ◽  
Intrauterine Contraceptive ◽  
Uterine Fluid ◽  
Intrauterine Contraceptive Devices

Use of intrauterine contraceptive devices (IUDs) is an effective method of reproduction control. But prolonged exposure to the uterine fluid generally results in the depositon of various organic and inorganic substances on their surfaces. This crust can harbor bacteria which may be responsible for IUD associated infectious diseases. We examined used IUDs by light microscopy, scanning and transmission electron microscopy and analyzed their crust by x-ray microanalysis and x-ray diffraction to better understand the process of IUD encrustation.Plastic IUD’s of Lippes loop type extracted after uterine exposure of various lengths of time, with obvious surface encrustations were fixed and processed for electron microscopy. A brand new Lippes loop and a used one after crust removal were exposed to vapors of 1% osmium tetroxide for 2 hours and examined by naked eye as well as analyzed by x-ray microanalysis.IUD surfaces were unevenly encrusted, areas of heavy encrustation alternated with areas of no obvious encrustation at all.

scholarly journals Aberrant Expression of Fgl-1 and Lag-3 in Adenomyosis

2021 ◽  
Author(s):  
Wenjing Sun ◽  
Xiaoyan Qin ◽  
Guangxi Yuan ◽  
Na Li ◽  
Junhui Liang ◽  
...  
Keyword(s):  
Uterine Cavity ◽  
Lymphocyte Activation ◽  
Secretory Phase ◽  
Normal Endometrium ◽  
Aberrant Expression ◽  
Proliferative Phase ◽  
Cell Immunity ◽  
Significant Difference ◽  
Mechanical Factors ◽  
Ectopic Endometrium

Abstract Background: The presence of ectopic functional endometrial glands and stroma in the myometrium of the uterine cavity is considered as adenomyosis. Various inflammatory, vascular and mechanical factors are involved in the symptoms and evolution of this pathology. Lymphocyte-activation gene 3 (Lag-3) is an immune inhibitory receptor and fibrinogen-like protein 1 (Fgl-1) is a major functional ligand of Lag-3. The binding of Lag-3 and Fgl-1 leads to inhibition of T-cell immunity, which is an important target of immunotherapy. The objective of this study was to evaluate the expression of Lag-3 and Fgl-1 in normal endometrium and adenomyosis.Methods: The expression of the Lag-3 and Fgl-1 in normal endometrium (proliferative phase: n=15; secretory phase: n=15) and adenomyotic endometrium (proliferative phase: n=15; secretory phase: n=15) were determined using immunohistochemistry and immunofluorescence analysis.Results: In normal and adnomyotic endometrium, no significant difference of Fgl-1 expression was noted between proliferative and secretory phases. Compared with normal endometrium, eutopic and ectopic endometrium of adenomyosis showed increased expression of Fgl-1. Lag-3 was almost negative in endometrial glands of normal and adenomyosis. Compared with normal endometrium, Lag-3 positive T-lymphocytes were more common in the stroma of adenomyosis.Conclusions: Our data suggest that aberrant expression of Lag-3 and Fgl-1 is present in the eutopic and ectopic endometrium of adenomyosis. We conclude that Lag-3/Fgl-1 signaling may be involved in the pathogenesis and development of adenomyosis.

scholarly journals A prospective interventional study of intra-caesarean copper intrauterine contraceptive devices insertion in a teaching hospital in a rural medical college in Telangana, India

2020 ◽  
Vol 9 (4) ◽  
pp. 1470
Author(s):  
Gillella Vijayalakshmi ◽  
Kavitha Kothapally ◽  
Uma Bhashyakarla ◽  
Vasantha Kavati
Keyword(s):  
Uterine Cavity ◽  
Postpartum Contraception ◽  
Medical College ◽  
Contraceptive Devices ◽  
Intrauterine Contraceptive ◽  
One Year ◽  
Long Acting ◽  
Long Acting Reversible Contraceptive

Background: Providing quality contraceptive services to women is essential for achieving maternal and child health. Objective of this study was to evaluate the efficacy of intra-caesarean insertion of copper IUCDs as postpartum contraception. To study the side effects of intra caesarean copper device. To study the continuation rates of intra-caesarean copper IUCDs. To study the acceptability of intra-caesarean copper IUCD as immediate postpartum contraceptive.Methods: The prospective study was undertaken at Bhaskar medical college and general hospital, Yenkepally, Moinabad, Telangana, between January 2016 and March 2018 after ethical committee clearance. About 60 pregnant women were enrolled into the study after an informed written consent regarding the procedure, benefits and complications and the need for follow up for at least one year. Copper T 380A/multiload copper 375 was inserted into the uterine cavity after delivery of the placenta and membranes during caesarean section. Patients were followed up at 6 weeks, 6 months and one-year intervals for any complaints, visibility of threads and for ultrasound examination for position of copper IUCDs in the uterus. Data analysis was done using Microsoft excel 2016.Results: Nearly 48.33% continued intra-caesarean copper IUCDs for more than 1 year. 70% did not have any complaints. 86.67% came for more than one follow-up visit. 47% had copper IUD threads visible by one year. No case of perforation either during insertion or during continuation was noted. None conceived with copper IUCD in situ. Removal of copper IUCD was also easy and none required hysteroscopic removal.Conclusions: Intra-caesarean copper IUCD insertion is a safe and effective long acting reversible contraceptive method in the postpartum period.

Selection and Preparation of Specific Tissue Regions For Tem Using Large Epoxy-Embedded Blocks

1973 ◽  
Vol 31 ◽  
pp. 324-325 ◽  
Author(s):  
P. M. Lowrie ◽  
W. S. Tyler
Keyword(s):  
Electron Microscopy ◽  
Anatomical Structures ◽  
Ultrastructural Studies ◽  
Transmission Electron ◽  
Microscopic Evaluation ◽  
Embedded Blocks ◽  
Specific Tissue ◽  
Definition Of ◽  
Areas Of Interest ◽  
Selection Of

The importance of examining stained 1 to 2μ plastic sections by light microscopy has long been recognized, both for increased definition of many histologic features and for selection of specimen samples to be used in ultrastructural studies. Selection of specimens with specific orien ation relative to anatomical structures becomes of critical importance in ultrastructural investigations of organs such as the lung. The uantity of blocks necessary to locate special areas of interest by random sampling is large, however, and the method is lacking in precision. Several methods have been described for selection of specific areas for electron microscopy using light microscopic evaluation of paraffin, epoxy-infiltrated, or epoxy-embedded large blocks from which thick sections were cut. Selected areas from these thick sections were subsequently removed and re-embedded or attached to blank precasted blocks and resectioned for transmission electron microscopy (TEM).

Ultrastructural studies of the relationship between actin filaments and secretory granules

1990 ◽  
Vol 48 (3) ◽  
pp. 458-459
Author(s):  
Ellen Holm Nielsen
Keyword(s):  
Electron Microscopy ◽  
Colloidal Gold ◽  
Actin Filaments ◽  
Secretory Granules ◽  
Secretory Cells ◽  
Ultrastructural Studies ◽  
Transmission Electron ◽  
Granule Membrane ◽  
Ultrathin Sections ◽  
Lowicryl K4m

In secretory cells a dense and complex network of actin filaments is seen in the subplasmalemmal space attached to the cell membrane. During exocytosis this network is undergoing a rearrangement facilitating access of granules to plasma membrane in order that fusion of the membranes can take place. A filamentous network related to secretory granules has been reported, but its structural organization and composition have not been examined, although this network may be important for exocytosis.Samples of peritoneal mast cells were frozen at -70°C and thawed at 4°C in order to rupture the cells in such a gentle way that the granule membrane is still intact. Unruptured and ruptured cells were fixed in 2% paraformaldehyde and 0.075% glutaraldehyde, dehydrated in ethanol. For TEM (transmission electron microscopy) cells were embedded in Lowicryl K4M at -35°C and for SEM (scanning electron microscopy) they were placed on copper blocks, critical point dried and coated. For immunoelectron microscopy ultrathin sections were incubated with monoclonal anti-actin and colloidal gold labelled IgM. Ruptured cells were also placed on cover glasses, prefixed, and incubated with anti-actin and colloidal gold labelled IgM.

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