Electron Microscopy in Surgical Pathology

1972 ◽  
Vol 30 ◽  
pp. 86-87
Author(s):  
Joseph A. Lynn
Keyword(s):  
Electron Microscopy ◽  
Basement Membrane ◽  
Membranous Glomerulonephritis ◽  
Chronic Glomerulonephritis ◽  
Acute Glomerulonephritis ◽  
Diabetic Glomerulosclerosis ◽  
Electron Microscopic ◽  
Microscopic Evaluation ◽  
Focal Glomerulonephritis ◽  
Idiopathic Membranous Glomerulonephritis

Utilization of paraformaldehyde or even formalin fixed surgical specimens in combination with rapid (24 hour) processing techniques allow for electron microscopic evaluation of a number of difficult diagnostic problems in histopathology. The principle diagnostic uses of electron microscopy at the present time are in the following areas: 1) Renal biopsies with deviations from the normal observed primarily in glomeruli (tubular abnormalities have been subjected to less study) including, degree of cellularity, abnormalities (primarily thickening) of the glomerular basement membrane, deposits of various sorts in juxtaposition or within the basement membrane, accumulations of mesangial matrix or “glomerular scar fiber,” cellular changes including fusion of foot processes, and more recently demonstration of viral agents in the following lesions; acute glomerulonephritis, lipoid nephrosis, chronic glomerulonephritis, focal glomerulonephritis, idiopathic membranous glomerulonephritis, lupus nephritis, diabetic glomerulosclerosis, eclampsia, and amyloidosis;

The blister of porphyria cutanea tarda: A Scanning and Transmission Electron Microscopic study

1986 ◽  
Vol 44 ◽  
pp. 334-335
Author(s):  
Veronika Burmeister ◽  
R. Swaminathan
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Basement Membrane ◽  
Middle Age ◽  
Porphyria Cutanea Tarda ◽  
Minor Trauma ◽  
Electron Microscopic ◽  
Transmission Electron Microscopic Study ◽  
Transmission Electron ◽  
Transmission Electron Microscopic

Porphyria cutanea tarda (PCT) is a disorder of porphyrin metabolism which occurs most often during middle age. The disease is characterized by excessive production of uroporphyrin which causes photosensitivity and skin eruptions on hands and arms, due to minor trauma and exposure to sunlight. The pathology of the blister is well known, being subepidermal with epidermodermal separation, it is not always absolutely clear, whether the basal lamina is attached to the epidermis or the dermis. The purpose of our investigation was to study the attachment of the basement membrane in the blister by comparing scanning with transmission electron microscopy.

scholarly journals Analytical electron microscopic evaluation of the effects of paraformaldehyde pretreatment on the reaction of glutaraldehyde with biogenic amines.

1982 ◽  
Vol 30 (5) ◽  
pp. 481-486 ◽  
Author(s):  
R E McClung ◽  
J Wood
Keyword(s):  
Electron Microscopy ◽  
Analytical Electron Microscopy ◽  
Microscopic Level ◽  
Electron Microscopic Level ◽  
Spectrographic Analysis ◽  
Electron Microscopic ◽  
X Ray ◽  
Microscopic Evaluation ◽  
Analytical Electron ◽  
Dense Product

Analytical electron microscopy was used to determine the quantitative effects of paraformaldehyde pretreatment on the formation of the biogenic amine-glutaraldehyde-chrome complex. Pretreatment with paraformaldehyde prevented the glutaraldehyde-chrome reaction with norepinephrine in the rat adrenal medulla. In contrast to the effect of paraformaldehyde on norepinephrine, pretreatment did not prevent the chrome reaction in serotonin-containing argentaffin cells of the gut. X-Ray energy spectrographic analysis revealed a significant decrease in chrome content in the paraformaldehyde treated tissue, but sufficient chrome did react to produce an electron-dense product. Thus by treating tissue with paraformaldehyde prior to the glutaraldehyde chrome procedure, serotonergic sites may be differentiated from catecholaminergic areas at the electron microscopic level.

scholarly journals THE GLOMERULUS IN EXPERIMENTAL RENAL DISEASE IN RATS AS OBSERVED BY LIGHT AND ELECTRON MICROSCOPY

1955 ◽  
Vol 102 (5) ◽  
pp. 573-580 ◽  
Author(s):  
Carolyn F. Piel ◽  
Luther Dong ◽  
F.W.S. Modern ◽  
Joseph R. Goodman ◽  
Roger Moore
Keyword(s):  
Electron Microscopy ◽  
Endothelial Cells ◽  
Basement Membrane ◽  
Light Microscopy ◽  
Light And Electron Microscopy ◽  
Electron Microscopic ◽  
Crescent Formation ◽  
Colloidal Iron ◽  
Narrow Channels ◽  
Glomerular Capillary

Nephrotoxic serum disease in rats has been studied by light and electron microscopy from 1 hour to 10 weeks after production of the disease. By light microscopy leucocytic infiltration of the glomerular capillary was observed between the 3rd and 6th hour. At 6 hours an increase in colloidal iron-positive material was observed coating the extraluminal surface of the capillaries. Also at this time swelling of the endothelial cells becomes prominent. By 72 hours, thickening of the basement membrane was observed. Glomerular capillary thrombi were observed in approximately half the tissue examined in the first 2 weeks of disease. 50 per cent of the animals showed severe chronic lesions, exudation into the capsular space, crescent formation, and obliteration of glomeruli. At 1 hour electron microscopic pictures showed that osmophilic material may line the foot processes of the epithelial cells and obliterate all but narrow channels of the space between the feet. By 6 hours thickening of the basement membrane was prominent. This change persisted throughout 10 weeks of observation. The tissue from animals which had severe chronic alterations by light microscopy revealed changes which could not be interpreted at this time.

scholarly journals ELECTRON MICROSCOPIC LOCALIZATION OF THE NEPHROTOXIC ANTIBODY IN THE GLOMERULI OF THE RAT AFTER INTRAVENOUS APPLICATION OF PURIFIED NEPHRITOGENIC ANTIBODY-FERRITIN CONJUGATES

1968 ◽  
Vol 127 (5) ◽  
pp. 867-878 ◽  
Author(s):  
Arnold Vogt ◽  
Hermann Bockhorn ◽  
Keniti Kozima ◽  
Masamichi Sasaki
Keyword(s):  
Electron Microscopy ◽  
Basement Membrane ◽  
Intravenous Injection ◽  
Fluorescent Antibody ◽  
Fluorescent Antibody Technique ◽  
Electron Microscopic ◽  
Antibody Technique ◽  
Disappearance Time ◽  
Filtration Surface ◽  
Electron Microscopic Localization

Nephritis in rats was induced by intravenous injection of purified ferritin-conjugated rabbit and duck nephrotoxic globulin. Using the fluorescent antibody technique, the same capillary pattern was found as that in glomeruli of rats receiving uncoupled nephrotoxic globulin. Electron microscopy revealed a heavy accumulation of the basement membrane-fixed antibody almost exclusively at the endothelial side. A higher concentration of ferritin was demonstrable in the peripheral basement membrane. The once-fixed antibody remained at the site of reaction though decreasing with time. The half-disappearance time seemed to be shorter than that of the uncoupled nephrotoxic globulin. No difference in localization was observed between rabbit and duck antibody. At least 40 basement membrane-fixed antibody molecules from the rabbit per 3000 mµ2 of filtration surface were needed to cause immediate nephritis. To induce nephritis using duck antibody, a greater number of basement membrane-fixed antibody seemed to be necessary. No evidence of specific reaction with constituents of glomerular cells was obtained.

Sclerosing Glomerulonephritis

1975 ◽  
Vol 33 ◽  
pp. 408-409
Author(s):  
A. Mandal ◽  
K. Chrysant ◽  
J. Nordquist ◽  
S. Kraikitpanitch ◽  
D. Xoung ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Renal Failure ◽  
Retrospective Study ◽  
Light Microscopy ◽  
Membranous Glomerulonephritis ◽  
Chronic Glomerulonephritis ◽  
Previous Diagnosis ◽  
Renal Biopsies ◽  
End Stage ◽  
Made In

A small but undefined percentage of adults with idiopathic proteinuria, microscopic hematuria and hypertension with partial or no response to corticosteroid or immunosuppressive therapy, progress slowly to renal failure. Histological diagnosis of membranous, membranoproliferative or chronic glomerulonephritis were made in these patients. Retrospective reevaluation of renal pathology in such patients has resulted in the emergence of a new clinicopathological entity. We are reporting a retrospective study of renal biopsies using light microscopy (LM) and electron microscopy (EM) from six patients with previous diagnosis of proliferative glomerulonephritis (2 patients), membranous glomerulonephritis (2 patients), nephrosclerosis (1 patient) and end stage kidney (1 patient). These patients were aged between l6 and 51 years, four males and two females. They had initial average 24 hour proteinuria of 1.3 gm (range 0.5-3.4 gm) and blood urea nitrogen of 24 mgm percent (range 12-32 mgm percent).

The Connective Tissue Matrix of Cartilage as Revealed by Standard Fixation, Ruthenium Staining, High-Pressure Freezing, and Embedding in Water Soluble Media: A Comparison of Methods

1990 ◽  
Vol 48 (2) ◽  
pp. 326-327
Author(s):  
Douglas R. Keene
Keyword(s):  
Electron Microscopy ◽  
High Pressure ◽  
Freeze Substitution ◽  
Uranyl Acetate ◽  
Ruthenium Red ◽  
Water Soluble ◽  
High Pressure Freezing ◽  
Electron Microscopic ◽  
Microscopic Evaluation ◽  
Embedding Medium

Proteoglycan is a major component of the cartilage extracellular matrix, and the overall structure of this anionic molecule is highly dependent on the hydrated environment of cartilage. Without specific stabilization, proteoglycans are extracted or collapsed during deydration while processing for electron microscopy. The purpose of these experiments is to determine a method by which the structure of proteoglycans might be stabilized for electron microscopic evaluation.Chick sternal cartilage was prepared for transmission electron microscopy by the following methods and the resultant tissue ultrastructure compared: A) 1.5/1.5% gluteraldehyde/paraformaldehyde and 1% OsO4 fixation, dehydration in ethanol, propylene oxide, and embedding in Spurrs epoxy B) Fixation as in (A) directly followed by infiltration and embedding in Hexamethylol-melamine-methyl-ether (a water soluble embedding medium) trade name “nanoplast” C) Fixation by high pressure freezing followed by freeze substitution in acetone/OsO4 prior to embedding in epon 812. In variations of methods A and B above, ruthenium red (RR, 1500 ppm) or ruthenium hexamine trichloride (RHT, 6000 ppm) were added to the primary and secondary fixatives. All tissue sections were stained in uranyl acetate and lead citrate.

scholarly journals ELECTRON MICROSCOPIC STUDIES OF EXPERIMENTAL NEPHRITIS WITH FERRITIN-CONJUGATED ANTIBODY

1962 ◽  
Vol 115 (5) ◽  
pp. 929-936 ◽  
Author(s):  
Giuseppe A. Andres ◽  
Councilman Morgan ◽  
Konrad C. Hsu ◽  
Richard A. Rifkind ◽  
Beatrice C. Seegal
Keyword(s):  
Electron Microscopy ◽  
Basement Membrane ◽  
Rat Kidney ◽  
Fluorescent Antibody ◽  
Ultrastructural Level ◽  
Fluorescent Antibody Technique ◽  
Electron Microscopic ◽  
Antibody Technique ◽  
Experimental Nephritis ◽  
Microscopic Studies

Ferritin-conjugated antibody has been used to identify by electron microscopy the sites at which nephrotoxic globulins localize in rat kidney during acute experimental glomerulonephritis. Antibody was concentrated in the glomerular basement membrane and in basement membrane-like material contained in distended cisternae of the endoplasmic reticulum. These data confirm and amplify, at the ultrastructural level, the results of studies obtained with the fluorescent antibody technique, and are consistent with the hypothesis that the cisternae and capillary basement membrane possess common proteins.

A Scanning Electron Microscopic Study on the Initial Invasion of Ehrlich Ascites (EA) Tumor Cells in Peritoneal Layer

1982 ◽  
Vol 40 ◽  
pp. 228-229
Author(s):  
E.C. Chew ◽  
E.C.V. Ooi ◽  
O.W. Lam ◽  
S.M. Kwan
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Basement Membrane ◽  
Tumor Cells ◽  
Present Communication ◽  
Electron Microscopic ◽  
Scanning Electron Microscopic Study ◽  
Ehrlich Ascites ◽  
Scanning Electron Microscopic ◽  
Scanning Electron

Several investigators have reported the invasive processes of tumor cells on the exposed basement membrane or injured peritoneum, however the detail of how the tumor cells adhere to the mesothelium and basement membrane has not been demonstrated. The present communication reveals the detailed process of the adhesion of EA tumor cells in the peritoneal layer by scanning electron microscopy.

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