A Scanning Electron Microscopic Evaluation of Section and Film Mounting for Transmission Electron Microscopy

1973 ◽  
Vol 48 (6) ◽  
pp. 337-342 ◽  
Author(s):  
Robert G. Summers ◽  
Paul C. Rusanowski
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Electron Microscopic ◽  
Transmission Electron ◽  
Microscopic Evaluation ◽  
Scanning Electron Microscopic ◽  
Scanning Electron

A Scanning Electron Microscopic Study of the Nephrotic Kidney

1974 ◽  
Vol 32 ◽  
pp. 10-11
Author(s):  
Peter M. Andrews
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Electron Microscopic ◽  
Vascular Perfusion ◽  
Transmission Electron ◽  
Scanning Electron Microscopic Study ◽  
Scanning Electron Microscopic ◽  
Aminonucleoside Nephrosis ◽  
Scanning Electron

Scanning electron microscopy, supplemented with light microscopy and transmission electron microscopy, was used to study aminonucleoside nephrosis in rats. Aminonucleoside nephrosis was induced by daily injections of puromycin aminonucIeoside (1.5 mg/10 gm). When the rats exhibited considerable proteinuria (10 mg/ml after 14 days of injections), their kidneys were fixed by vascular perfusion, sectioned and critical point dried.

A Comparative Study of Fractographic Replicas

1974 ◽  
Vol 32 ◽  
pp. 566-567
Author(s):  
Loren Anderson ◽  
Pat Pizzo ◽  
Glen Haydon
Keyword(s):  
Electron Microscopy ◽  
Electron Microscopic Examination ◽  
Direct Examination ◽  
Electron Microscopic ◽  
Reliable Technique ◽  
Service Failures ◽  
Transmission Electron ◽  
Mode Of Failure ◽  
Scanning Electron Microscopic ◽  
Scanning Electron

Transmission electron microscopy of replicas has long been used to study the fracture surfaces of components which fail in service. Recently, the scanning electron microscope (SEM) has gained popularity because it allows direct examination of the fracture surface. However, the somewhat lower resolution of the SEM coupled with a restriction on the sample size has served to limit the use of this instrument in investigating in-service failures. It is the intent of this paper to show that scanning electron microscopic examination of conventional negative replicas can be a convenient and reliable technique for determining mode of failure.

scholarly journals Scanning Electron Microscopic Study of Modified Chloroplasts in Senescing Broccoli Florets

HortScience ◽  
2000 ◽  
Vol 35 (1) ◽  
pp. 99-103 ◽  
Author(s):  
Hirofumi Terai ◽  
Alley E. Watada ◽  
Charles A. Murphy ◽  
William P. Wergin
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Structural Changes ◽  
Freeze Fracture ◽  
Electron Microscopic ◽  
Transmission Electron ◽  
Scanning Electron Microscopic Study ◽  
Scanning Electron Microscopic ◽  
Scanning Electron

Structural changes in chloroplasts of broccoli (Brassica oleracea L., Italica group) florets during senescence were examined using light microscopy, scanning electron microscopy (SEM) with freeze-fracture technique, and transmission electron microscopy (TEM) to better understand the process of chloroplast degradation, particularly at the advanced stage of senescence. Light microscopy revealed that chloroplasts, which initially were intact and green, became obscure in shape, and their color faded during senescence. Small, colored particles appeared in cells as the florets approached the final stage of senescence and became full- to dark-yellow in color. Scanning electron microscopy showed that stroma thylakoids in the chloroplast initially were parallel to each other and grana thylakoids were tightly stacked. As senescence advanced, the grana thylakoids degenerated and formed globules. The globules became larger by aggregation as senescence progressed, and the large globules, called “thylakoid plexus,” formed numerous vesicles. The vesicles ultimately were expelled into the cytosol, and the light microscope revealed many colored particles in the senescent cells. These results indicate that the degradation of chloroplasts in broccoli florets progresses systematically, with the final product being colored particles, which are visible in yellow broccoli sepal cells.

A scanning electron microscopic method for assessing surface topography of bladders

1984 ◽  
Vol 42 ◽  
pp. 40-41
Author(s):  
Betty I. Tarnowski ◽  
Gregory R. Schonbaum
Keyword(s):  
Electron Microscopy ◽  
Electron Microscopic ◽  
Transmission Electron ◽  
Step Procedure ◽  
Critical Point Drying ◽  
Scanning Electron Microscopic ◽  
Sampling Procedures ◽  
Electron Microscopic Method ◽  
Scanning Electron ◽  
Sem Analyses

Neither light microscopy nor transmission electron microscopy lend themselves to an accurate assessment of focal changes of epithelium: both techniques are limited by sampling procedures. The same limitation, however, does not apply to scanning electron microscopy (SEM) (1,2) which permits statistically meaningful analyses on a larger number of samples. The usefulness of such an approach was explored in our studies on the induction of damage to rat urothelium by cyclophosphamide (3,4) and its prevention by adjunct therapy with 2,3-dimercaptopropane sulfonate (DMPS). Portions of the bladder were sampled from the dome, central region and trigone and the tissue was prepared for SEM by dehydration in acetone, followed by critical point drying and gold coating. SEM analyses were performed in a two-step procedure using a Novascan scanning electron microscope at low (20X) and high (100X) magnifications.

Lower extremity peripheral vascular disease. Part 2: Light and transmission electron microscopy

1989 ◽  
Vol 79 (12) ◽  
pp. 585-594
Author(s):  
DJ McCarthy ◽  
N Abell ◽  
T Reed
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Vascular Disease ◽  
Peripheral Vascular Disease ◽  
Peripheral Vascular ◽  
Electron Microscopic ◽  
Transmission Electron ◽  
Scanning Electron Microscopic ◽  
Microscopic Features ◽  
Conventional Light Microscopy

In spite of the most vigorous efforts to intervene medically and surgically when peripheral vascular disease threatens a patient, amputation of the extremity may be the only option left to arrest the progression of the disease. In a previous study, the authors assessed amputations, examined gross pathology, and identified scanning electron microscopic features associated with atherosclerotic disease. In the present study, the authors discuss this disease in terms of conventional light microscopy and transmission electron microscopy.

Electron Microscopic Study of the Polymyxin Treated Goat Erythrocytes

1984 ◽  
Vol 39 (7-8) ◽  
pp. 776-780
Author(s):  
T. K. Mandal ◽  
S. N. Chatterjee
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Spherical Shape ◽  
Polymyxin B ◽  
Electron Microscopic ◽  
Transmission Electron ◽  
Ultrathin Sections ◽  
Treatment Changes ◽  
Dose Dependent ◽  
Scanning Electron

Abstract Polymyxin B produced dose dependent changes in the surface topography of the goat erythrocyte cells. Transformation from the normal biconcave discs through crenated structures to the final rounded or spherical shape was recorded by scanning electron microscopy. A maxim um of three to four crenations per cell was recorded corresponding to a polymyxin dose of 15.62 ng/ml. Transmission electron microscopy of the ultrathin sections of treated or untreated erythrocytes indicated that the crenations were formed by protrusions of the plasma membrane, occurring presumably because of the local increase of membrane fluidity after polymyxin treatment. Changes in the shape of the erythrocytes to the ultimate rounded forms were also indicated by the transmission electron microscopy.

A comparative light and electron microscopic study of microspore and tapetal development in male fertile and cytoplasmic male sterile oilseed rape (Brassica napus)

1986 ◽  
Vol 64 (5) ◽  
pp. 1055-1068 ◽  
Author(s):  
I. Grant ◽  
W. D. Beversdorf ◽  
R. L. Peterson
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Brassica Napus ◽  
Microspore Development ◽  
Male Sterile ◽  
Electron Microscopic ◽  
Transmission Electron ◽  
Mother Cells ◽  
Scanning Electron

The cytological development of male cells and the tapetum of male fertile and combined cytoplasmic triazine-resistant cyto-plasmic-genetic male sterile (ctr) lines of B. napus L. was studied using light, scanning electron, and transmission electron microscopy. Development of the cytoplasmic-genetic male sterile anther was similar to the normal anther up to and including meiotic prophase I. After this stage, degeneration of the microspore mother cells occurs within the callose walls, and tetrads of microspores are not formed. These degenerating microspore mother cells appear to develop numerous endoplasmic reticulum derived vesiculated structures, which may be involved in lysis of organelles. Degeneration occurs simultaneously with a proliferation of the tapetum, which eventually fills the anther locule. It is not clear whether the abortion of the microspore mother cells during meiosis stimulates proliferation of the tapetum or whether the proliferating tapetum actually interferes with microspore development thereby causing degeneration. Dilated endoplasmic reticulum cisternae containing crystalline-like deposits, and plastids with osmiophilic bodies, are frequent in cells of the proliferated tapetum of cytoplasmic-genetic male sterile anthers.

Electron Microscopic Evaluation of Altered Collagen Structure Induced by N-Monochloroglycine (GK-101)

1977 ◽  
Vol 56 (12) ◽  
pp. 1539-1545 ◽  
Author(s):  
J.H. Kronman ◽  
M. Goldman ◽  
C.M. Habib ◽  
L. Mengel
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Collagen Fiber ◽  
Collagen Structure ◽  
Fiber Structure ◽  
Electron Microscopic ◽  
Transmission Electron ◽  
Microscopic Evaluation

The effects of N-monochloroglycine (NMG) solutions on the structure of collagen were studied by transmission electron microscopy. In addition to finding that collagen structure was altered by NMG solutions, the results offer evidence that buffer and water also exert some effect on collagen fiber structure.

Two- or three-way observations of the identical cell elements within the same sections by LM, TEM and/or SEM

1978 ◽  
Vol 36 (2) ◽  
pp. 82-83 ◽  
Author(s):  
Nakazo Watari ◽  
Yasuaki Hotta ◽  
Yoshio Mabuchi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fine Structure ◽  
Light Microscopy ◽  
Thin Sections ◽  
Transmission Electron ◽  
Identical Cell ◽  
Electron Microscopes ◽  
Scanning Electron

It is very useful if we can observe the identical cell elements within the same sections by light microscopy (LM), transmission electron microscopy (TEM) and/or scanning electron microscopy (SEM) sequentially, because, the cell fine structure can not be indicated by LM, while the color is; on the other hand, the cell fine structure can be very easily observed by EM, although its color properties may not. However, there is one problem in that LM requires thick sections of over 1 μm, while EM needs very thin sections of under 100 nm. Recently, we have developed a new method to observe the same cell elements within the same plastic sections using both light and transmission (conventional or high-voltage) electron microscopes.In this paper, we have developed two new observation methods for the identical cell elements within the same sections, both plastic-embedded and paraffin-embedded, using light microscopy, transmission electron microscopy and/or scanning electron microscopy (Fig. 1).

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