Aberrant Type C Virus Production in a Cell Line Derived from Balb/3T3

1972 ◽  
Vol 30 ◽  
pp. 286-287
Author(s):  
N. Savage ◽  
A. Hackett
Keyword(s):  
Electron Microscopy ◽  
Cell Line ◽  
Leukemia Virus ◽  
Morphological Characteristics ◽  
Virus Production ◽  
Oncogenic Viruses ◽  
Endogenous Virus ◽  
Virus Particles ◽  
Moloney Leukemia Virus ◽  
A Cell

A cell line, UC1-B, which was derived from Balb/3T3 cells, maintains the same morphological characteristics of the non-transformed parental culture, and shows no evidence of spontaneous virus production. Survey by electron microscopy shows that the cell line consists of spindle-shaped cells with no unusual features and no endogenous virus particles.UC1-B cells respond to Moloney leukemia virus (MLV) infection by a change in morphology and growth pattern which is typical of cells transformed by sarcoma virus. Electron microscopy shows that the cells are now variable in shape (rounded, rhomboid, and spindle), and each cell type has some microvilli. Virtually all (90%) of the cells show virus particles developing at the cell surface and within the cytoplasm. Maturing viruses, typical of the oncogenic viruses, are found along with atypical tubular forms in the same cell.

Expression of viral particles by raw 264.7 mouse macrophage cells after treatment with lipopolysaccharide

1990 ◽  
Vol 48 (3) ◽  
pp. 574-575
Author(s):  
Robert Blystone ◽  
Johnathan Kiel ◽  
Jill Parker ◽  
Chris Collumbs
Keyword(s):  
Leukemia Virus ◽  
Virus Production ◽  
Raw 264.7 ◽  
Mouse Macrophage ◽  
Type Virus ◽  
Virus Particles ◽  
Viral Particles ◽  
Moloney Leukemia Virus ◽  
Abelson Virus ◽  
Fibroblast Transformation

The RAW 264.7 mouse macrophage cell line “was established from the ascites of a tumor induced in a male BAB/14 mouse by the intraperitoneal injection of A-MuLV.” Abelson murine leukemia virus (A-MuLV) is an RNA retrovirus that can cause transformation of certain cells. The AMuLV RNA is 5,600 bases long and forms the familiar nucleoid associated with C-type virus morphology. Raschke et al. further stated that “the other macrophage line, RAW 264, fails to secrete detectable virus particles and is negative in the XC plaque formation assay, as well as the fibroblast transformation assay for Abelson virus but becomes positive for Abelson virus production after rescue by Moloney leukemia virus.” Contrary to the original citation, we are reporting that lipopolysaccharide treated RAW cells will shed C-type virus without a Moloney rescue.

Head Size and DNA Content in Bacteriophage T4

1972 ◽  
Vol 30 ◽  
pp. 200-201
Author(s):  
Fred Eiserling ◽  
A. H. Doermann ◽  
Linde Boehner
Keyword(s):  
Electron Microscopy ◽  
Dna Content ◽  
Bacteriophage T4 ◽  
Head Size ◽  
Virus Particles ◽  
Altered Protein ◽  
A Cell ◽  
Bacterial Viruses ◽  
A Site ◽  
Protein Shell

The control of form or shape inheritance can be approached by studying the morphogenesis of bacterial viruses. Shape variants of bacteriophage T4 with altered protein shell (capsid) size and nucleic acid (DNA) content have been found by electron microscopy, and a mutant (E920g in gene 66) controlling head size has been described. This mutant produces short-headed particles which contain 2/3 the normal DNA content and which are non-viable when only one particle infects a cell (Fig. 1).We report here the isolation of a new mutant (191c) which also appears to be in gene 66 but at a site distinct from E920g. The most striking phenotype of the mutant is the production of about 10% of the phage yield as “giant” virus particles, from 3 to 8 times longer than normal phage (Fig. 2).

scholarly journals Primer Binding Site-Dependent Restriction of Murine Leukemia Virus Requires HP1 Binding by TRIM28

2008 ◽  
Vol 82 (9) ◽  
pp. 4675-4679 ◽  
Author(s):  
Daniel Wolf ◽  
Florence Cammas ◽  
Régine Losson ◽  
Stephen P. Goff
Keyword(s):  
Binding Site ◽  
Cell Line ◽  
Murine Leukemia Virus ◽  
Leukemia Virus ◽  
Embryonic Stem ◽  
Transcriptional Silencing ◽  
Primer Binding Site ◽  
A Cell ◽  
Ec Cells ◽  
Murine Leukemia

ABSTRACT TRIM28 is a transcriptional corepressor which is required for primer binding site (PBS)-dependent restriction of murine leukemia virus (MLV) replication in embryonic stem and embryonic carcinoma (EC) cells. PBS-dependent restriction of MLV leads to transcriptional silencing of the integrated provirus and has been shown to correlate with TRIM28-mediated recruitment of HP1 to the silenced loci. Here we show, using a cell line with a point mutation in the HP1 binding domain of TRIM28, that interaction with HP1 is absolutely required for the PBS-dependent restriction of MLV in the F9 EC cell line.

Detection of Influenza Virus Particles in Throat Washings by Electron Microscopy

1970 ◽  
Vol 28 ◽  
pp. 340-341
Author(s):  
Mahadeo B. Pendharker ◽  
Kenneth A. Siegesmund ◽  
Harold D. Rose ◽  
Frank Piraino ◽  
Ross C. Kory
Keyword(s):  
Electron Microscopy ◽  
Influenza Virus ◽  
Ultrastructural Study ◽  
Filter Paper ◽  
Influenza A ◽  
Laboratory Tests ◽  
Cell Block ◽  
Virus Particles ◽  
Centrifuge Tube ◽  
A Cell

Although the symptomatology of influenza is well known and fairly typical in most cases, the exact diagnosis is often presumptive because of the technical difficulties in performing laboratory tests. We have recently developed a method for concentrating and processing throat washings to provide a cell block which can be sectioned for ultrastructural study. We have used this technique for studying the throat washings from five sero-logically proven patients with Influenza A2 Hong Kong infection. Two ml of throat washings were mixed with 0. 5 ml of a fixative containing 2% acrolein, 3% glutaraldehyde in phosphate buffer at pH 7. 2 in a centrifuge tube. A disc of filter paper (Whatman #41 ashless) 5 mm in diameter was placed in the centrifuge tube, and the samples were centrifuged at 18,000 rpm for one hour.

Establishment of cell clone of lymphoma and a cell line infected with leukemia virus and study on its inducted differentiation

1992 ◽  
Vol 4 (3) ◽  
pp. 61-65
Author(s):  
Li Cheng ◽  
Xianshou Kong ◽  
Xiaoyuan Liu ◽  
Han Xu ◽  
Ping Deng ◽  
...  
Keyword(s):  
Cell Line ◽  
Cell Clone ◽  
Leukemia Virus ◽  
A Cell

scholarly journals CrFK Feline Kidney Cells Produce an RD114-Like Endogenous Virus That Can Package Murine Leukemia Virus-Based Vectors

1998 ◽  
Vol 72 (9) ◽  
pp. 7685-7687 ◽  
Author(s):  
Jörg G. Baumann ◽  
Walter H. Günzburg ◽  
Brian Salmons
Keyword(s):  
Cell Line ◽  
Kidney Cell ◽  
Murine Leukemia Virus ◽  
Leukemia Virus ◽  
Moloney Murine Leukemia Virus ◽  
Viral Infectivity ◽  
Kidney Cells ◽  
Kidney Cell Line ◽  
Endogenous Virus ◽  
Murine Leukemia

ABSTRACT The feline kidney cell line CrFK is used extensively for viral infectivity assays and for study of the biology of various retroviruses and derived vectors. We demonstrate the production of an endogenous, RD114-like, infectious retrovirus from CrFK cells. This virus also is shown to efficiently package Moloney murine leukemia virus vectors.

Immunoelectron Microscopy of Type C Virus Particles Produced in a Cell Line Derived from Pleural Effusion of a Lymphoma Patient

1971 ◽  
Vol 29 ◽  
pp. 374-375
Author(s):  
T. Shigematsu ◽  
E. S. Priori ◽  
L. Dmochowski ◽  
J. R. Wilbur
Keyword(s):  
Pleural Effusion ◽  
Cell Line ◽  
Monolayer Culture ◽  
Human Tumor ◽  
Tumor Cell Line ◽  
Lymphoma Patient ◽  
Human Tumor Cell ◽  
Virus Particles ◽  
A Cell ◽  
Type C

A monolayer culture (ESP-1) was established with cells obtained from pleural effusion of a patient with Burkitt lymphoma (American type). Virus particles, similar to murine type C virus particles, were first observed in cells of the 10th passage of the culture. Mature and immature, including budding, virus particles were frequently found in all subsequent passages of this continuous culture (including 28th).A human tumor cell line continuously producing type C virus particles provides a valuable new system for immunological studies of such virus particles.

Sign in / Sign up

Export Citation Format

Share Document