Scanning Electron Microscopy of Fish Scales as an Adjunctive Aid in Speciation

1972 ◽  
Vol 30 ◽  
pp. 394-395
Author(s):  
Edward D. DeLamater ◽  
Walter R. Courtenay ◽  
Cecil Whitaker
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Lateral Line ◽  
Species Differences ◽  
Fish Scale ◽  
Fish Scales ◽  
Anterior Border ◽  
The University ◽  
Multiple Holes ◽  
Scanning Electron

Comparative scanning electron microscopy studies of fish scales of different orders, families, genera and species within genera have demonstrated differences which warrant elaboration. These differences in detail appear to be sufficient to act as “fingerprints”, at least, for family differences. To date, the lateral line scales have been primarily studied. These demonstrate differences in the lateral line canals; the pattern of ridging with or without secondary protuberances along the edges; the pattern of spines or their absence on the anterior border of the scales; the presence or absence of single or multiple holes on the ventral and dorsal sides of the lateral line canal covers. The distances between the ridges in the pattern appear likewise to be important.A statement of fish scale structure and a comparison of family and species differences will be presented.The authors wish to thank Dr. Donald Marzalek and Mr. Wallace Charm of the Marine and Atmospheric Laboratory of the University of Miami and Dr. Sheldon Moll and Dr. Richard Turnage of AMR for their exhaustive help in these preliminary studies.

SEM analysis of fish scale cross sections: A technique study

1992 ◽  
Vol 50 (1) ◽  
pp. 744-745
Author(s):  
M.E. Lee ◽  
A. Moller ◽  
P.S.O. Fouche ◽  
I.G Gaigher
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cross Sections ◽  
Soft Tissues ◽  
Close Association ◽  
Sem Analysis ◽  
Fish Scale ◽  
Fish Scales ◽  
Micro Structures ◽  
Scanning Electron

Scanning electron microscopy of fish scales has facilitated the application of micro-structures to systematics. Electron microscopy studies have added more information on the structure of the scale and the associated cells, many problems still remain unsolved, because of our incomplete knowledge of the process of calcification. One of the main purposes of these studies has been to study the histology, histochemistry, and ultrastructure of both calcified and decalcified scales, and associated cells, and to obtain more information on the mechanism of calcification in the scales. The study of a calcified scale with the electron microscope is complicated by the difficulty in sectioning this material because of the close association of very hard tissue with very soft tissues. Sections often shatter and blemishes are difficult to avoid. Therefore the aim of this study is firstly to develop techniques for the preparation of cross sections of fish scales for scanning electron microscopy and secondly the application of these techniques for the determination of the structures and calcification of fish scales.

scholarly journals A South American embalmed head

1970 ◽  
Vol 80 (1) ◽  
Author(s):  
Rudi Lallo ◽  
Roberto Testi ◽  
Massimo Massimelli ◽  
Raffaella Bianucci ◽  
Rosa Boano ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
3D Reconstruction ◽  
Ct Scanning ◽  
Facial Trauma ◽  
South American ◽  
X Rays ◽  
Age At Death ◽  
The University ◽  
Scanning Electron

The embalmed head of a young South American man is housed in the Museum of Anthropology and Ethnography of the University of Turin. This man, who suffered a violent facial trauma, was intentionally beheaded after his death, and his head was embalmed.   The aim of this study was to determine the age at death, the cause of death and the embalming techniques by means of CT scanning, 3D reconstruction, X-rays, histology, endoscopy and scanning electron microscopy followed by microanalysis.

The morphology of the pit organs and lateral line canal neuromasts of Mustelus antarcticus (Chondrichthyes: Triakidae)

2000 ◽  
Vol 80 (1) ◽  
pp. 155-162 ◽  
Author(s):  
Meredith B. Peach ◽  
Gregory W. Rouse
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Lateral Line ◽  
Hair Cells ◽  
Sensory System ◽  
Lateral Line Canal ◽  
Sensory Epithelia ◽  
Sensory Hairs ◽  
And Function ◽  
Scanning Electron

The pit organs (free neuromasts) of sharks are part of the lateral line sensory system, but there is still confusion about their exact morphology and function(s). This is partly because of reported physiological differences between the pit organs and the lateral line canal neuromasts, and partly because the morphology of pit organs has not been adequately documented. To compare their morphology, the pit organs and canal neuromasts of the gummy shark Mustelus antarcticus (Chondrichthyes: Triakidae) were examined using transmission and scanning electron microscopy. Both pit organs and canal neuromasts had hair cells with the `staircase' arrangement of sensory hairs (stereovilli) characteristic of vertebrate mechanoreceptors. Stereovilli bundles of different sizes were distributed haphazardly throughout the pit organs and canal neuromasts. The density of hair cells was similar in the pit organs and canal neuromasts, but differences in the overall size and/or shape of the sensory epithelia might account for some of the reported differences in mechanosensitivity.

Exine Ultrastructure of Myrtaceous Pollen

1982 ◽  
Vol 30 (1) ◽  
pp. 75 ◽  
Author(s):  
PA Gadek ◽  
HA Martin
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Species Differences ◽  
Transmission Electron Microscopy Analysis ◽  
Transmission Electron ◽  
Exine Ultrastructure ◽  
Electron Microscopy Analysis ◽  
Thin Electron ◽  
Microscopy Analysis ◽  
Scanning Electron

Exine ultrastructure has been determined by transmission electron microscopy analysis of the grains of a number of species of Myrtaceae, chosen to represent some of the diversity of sculpture and structure that can be observed using light microscopy and scanning electron microscopy. The species all show a typical angiosperm exine differentiation consisting of two chemically different layers, an electron-dense ektexine and a less dense endexine divided by a very thin electron-transparent lamella. They all differ, however, from the typical angiosperm architecture by the presence of a somewhat unstructured, granulate infratectal layer and a granular/alveolate endexinous layer around the pores. Species differences relate to the granulate coiumellate organization of the infractectal layer, the extent or density of tectal perforations and the presence and thickness of a foot layer around the grain.

scholarly journals Short Communication: Morphological study of the scales of Barbodes cf. binotatus (Cyprinidae) from Toba Lake, Indonesia by using Scanning Electron Microscopy-Energy Dispersive Spectroscopy (SEM-EDS)

2021 ◽  
Vol 22 (9) ◽  
Author(s):  
Khairiza Lubis ◽  
MUFTI SUDIBYO ◽  
ELIDA HAFNI SIREGAR ◽  
NURLAINI LAILI
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Lateral Line ◽  
Energy Dispersive Spectroscopy ◽  
Morphological Study ◽  
Short Communication ◽  
Morphological Characteristics ◽  
Energy Dispersive ◽  
Dorsal Fin ◽  
Scanning Electron

Abstract. Lubis K, Sudibyo M, Siregar EH, Laili N. 2021. Short Communication: Morphological study of the scales of Barbodes cf. binotatus (Cyprinidae) from Toba Lake, Indonesia by using Scanning Electron Microscopy-Energy Dispersive Spectroscopy (SEM-EDS). Biodiversitas 22: 3821-3828. Spotted barb (Barbodes cf binotatus) is a freshwater fish of Sumatra Island, especially found in the Toba. The study of morphological characteristics of the scales of Spotted barb (B. cf binotatus) is necessary in fish taxonomy. Therefore, the present study investigated the morphology and the chemical elements of the scales located between the dorsal fin and lateral line and those on the lateral line using SEM-EDS (Scanning Electron Microscope (SEM)- Energy Dispersive X-ray Spectroscopy (EDS). We found that the scales of Spotted barb (B. cf binotatus) have the general morphological characteristics of cycloid scales. The morphologies of the scales between the dorsal fin and lateral line and those on the lateral line are slightly different. Lateral line scales of Spotted barb (B. cf binotatus) have no focus area, replaced by a lateral line canal located along the anterior-posterior axis of the scales with two openings. The anterior opening is bigger and wider in compared to the posterior opening. The circuli of the dorsal fin scale is orientated towards the focus. However, the circuli of the lateral scale faces the lateral line canal. The scales contain some elements such as Ca, K, O, Mg, Na, and P.

Scanning Electron Microscopy of Scales and its Taxonomic Application in the Fish Genus Channa

2014 ◽  
Vol 20 (4) ◽  
pp. 1188-1197 ◽  
Author(s):  
Sudip Dey ◽  
Shyama P. Biswas ◽  
Samujjwal Dey ◽  
Shankar P. Bhattacharyya
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Relevant Literature ◽  
Radial Distance ◽  
Fish Scales ◽  
Taxonomic Significance ◽  
Circular Space ◽  
Space Width ◽  
Scanning Electron

AbstractScanning electron microscopy (SEM) of scales in six species of the fish genus Channa revealed certain features relevant to taxonomic significance. The location of focus, inter-radial distance and width of circuli, inter-circular space, width of radii, shape and size of lepidonts, etc. were found to be different in different species. The importance of SEM of scales in poorly understood taxonomy and phylogeny of the fish genus Channa is discussed with the help of relevant literature. Further, the role of SEM of fish scales for taxonomic applications is discussed in detail.

Pathogenesis of Human Hair Defects

1974 ◽  
Vol 32 ◽  
pp. 12-13
Author(s):  
P.S. Porter ◽  
T. Aoyagi ◽  
R. Matta
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Human Hair ◽  
Standard Techniques ◽  
Scanning Electron

Using standard techniques of scanning electron microscopy (SEM), over 1000 human hair defects have been studied. In several of the defects, the pathogenesis of the abnormality has been clarified using these techniques. It is the purpose of this paper to present several distinct morphologic abnormalities of hair and to discuss their pathogenesis as elucidated through techniques of scanning electron microscopy.

Ultrastructural Analysis of the Salivary Glands of Gromphadorhina Portentosa (Schaum)

1977 ◽  
Vol 35 ◽  
pp. 638-639
Author(s):  
P.J. Dailey
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Salivary Glands ◽  
Secretory Vesicles ◽  
The Past ◽  
Transmission Electron ◽  
Means Of Transmission ◽  
Gromphadorhina Portentosa ◽  
Scanning Electron ◽  
Topographical Relief

The structure of insect salivary glands has been extensively investigated during the past decade; however, none have attempted scanning electron microscopy (SEM) in ultrastructural examinations of these secretory organs. This study correlates fine structure by means of SEM cryofractography with that of thin-sectioned epoxy embedded material observed by means of transmission electron microscopy (TEM).Salivary glands of Gromphadorhina portentosa were excised and immediately submerged in cold (4°C) paraformaldehyde-glutaraldehyde fixative1 for 2 hr, washed and post-fixed in 1 per cent 0s04 in phosphosphate buffer (4°C for 2 hr). After ethanolic dehydration half of the samples were embedded in Epon 812 for TEM and half cryofractured and subsequently critical point dried for SEM. Dried specimens were mounted on aluminum stubs and coated with approximately 150 Å of gold in a cold sputtering apparatus.Figure 1 shows a cryofractured plane through a salivary acinus revealing topographical relief of secretory vesicles.

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