Re-Embedding of Specimens for Maximum Orientation

1972 ◽  
Vol 30 ◽  
pp. 696-697 ◽  
Author(s):  
Margot Brundage ◽  
Robert F. Dunn
Keyword(s):  
Tissue Block ◽  
Gas Flame

Re-embedding of plastic impregnated tissue blocks is easily accomplished when a combination of carnuba and Tenax dental waxes is used. This method is particularly helpful when combined with capsule flat end embedding, because the entire orientation range of the microtome can be utilized for final exact positioning of the specimen. A demonstration of both techniques will be given.The advantages of re-embedding are: 1, complete freedom in orientation of the tissue, and 2, multiple segments of the same block can be used for various orientations. Re-embedding involves: 1, removal of a tissue block from the surrounding plastic (Fig. 1), and 2, orientation and embedding of the tissue into wax which has suitable support properties for sectioning.Equipment and materials needed for re-embedding include: dissecting microscope; wax mixture ( carnuba 35%, Tenax 65%); empty gelatine capsules; dental carvers; dissecting needles; #7 Dupont forceps; Pasteur pipettes; specimen holders; razor blades; and gas flame.

A New Approach to the Demonstration of Oxidase-Localization Using Tannic Acid Or Catechin

1973 ◽  
Vol 31 ◽  
pp. 698-699
Author(s):  
V. Mizuhira ◽  
Y. Futaesaku
Keyword(s):  
Cross Section ◽  
Tannic Acid ◽  
Elastic Fiber ◽  
The Other ◽  
New Approach ◽  
Tissue Block ◽  
Active Reaction ◽  
The Cross

Previously we reported that tannic acid is a very effective fixative for proteins including polypeptides. Especially, in the cross section of microtubules, thirteen submits in A-tubule and eleven in B-tubule could be observed very clearly. An elastic fiber could be demonstrated very clearly, as an electron opaque, homogeneous fiber. However, tannic acid did not penetrate into the deep portion of the tissue-block. So we tried Catechin. This shows almost the same chemical natures as that of proteins, as tannic acid. Moreover, we thought that catechin should have two active-reaction sites, one is phenol,and the other is catechole. Catechole site should react with osmium, to make Os- black. Phenol-site should react with peroxidase existing perhydroxide.

Investigation of pre-sprayed powders for electric contact welding

2020 ◽  
pp. 143-149
Author(s):  
Dinar R. Masalimov ◽  
Roman R. Galiullin ◽  
Rinat N. Sayfullin ◽  
Azamat F. Fayurshin ◽  
Linar F. Islamov
Keyword(s):  
Adhesion Strength ◽  
Electrical Contact ◽  
Research Purpose ◽  
Flame Spraying ◽  
Powder Materials ◽  
Electric Contact ◽  
Metal Powders ◽  
Unstable Flow ◽  
Gas Flame ◽  
Contact Welding

There are a number of difficulties in the electrical contact welding of powder materials: shedding of powder from the surface of a cylindrical part, impossibility of hardening the layer during welding due to flushing of the powders with coolant and unstable flow of powder into the welding zone. One solution is pre-spraying the powder in some way. (Research purpose). The research purpose is investigating the possibility of electric contact welding of metal powders preliminarily sprayed by a gas-flame method, namely, adhesion strength and losses during preliminary gas-flame spraying of powders. (Materials and methods) Powders of grades PG-NA-01, PrKhIIG4SR, PRZh3.200.28 were sprayed onto flat samples of St3 steel, polished to a roughness of Ra 1.25. The strength of powder adhesion to the base was studied by the cut method. (Results and discussion) The percentage loss of the powder as a whole is 3-23 percent for all the distances studied. The greatest powder losses appear at a distance of more than 180 millimeter from the tip of the burner for powders of grades PG-NA-01 and PrKhIIG4SR. The smallest powder losses were observed for PrZh3.200.28 powder, which totaled 3-7 percent. The maximum adhesion strength of the sprayed powders to the surface was 22.1 megapascals' when spraying the PG-NA-01 powder. The adhesion strength of powders of the grades PrKhIIG4SR and PrZh3.200.28 is small and amounts to 0.2-3 megapascals'. (Conclusions) The use of preliminary flame spraying of powders for their further electric contact welding is possible using PG-NA-01 grade powder, while the best adhesion to the base (that is more than 20 megapascals') is achieved with a spraying distance of 120-140 millimeter. The smallest powder losses during flame spraying are achieved at a spraying distance of 100-160 centimeters', at which the powder loss for the studied grades was 4-12 percent.

Results of the experimental bits bench test, equipped with the compound built-up layers

2020 ◽  
pp. 52-56
Author(s):  
V.N. Gadalov ◽  
V. M. Brodsky ◽  
E.A. Filatov ◽  
A.V. Filonovic
Keyword(s):  
Tungsten Carbide ◽  
Laboratory Tests ◽  
Bench Test ◽  
Laboratory Studies ◽  
Induction Method ◽  
Gas Flame ◽  
Flame Method ◽  
Drilling Bit ◽  
Pilot Tests

The article shows the results of performance laboratory studies of compound built-up layers, as well as benches of the pilot tests of the drilling bit rolling cutters, equipped on the bit gage surface of the compound built-up layer; the hardening layers were located along the gage teeth contour. The cast tungsten carbide building-up welding on experimental bits was carried out using a gas-flame method. The induction method of building-up welding was implemented on the rolling cutters of the serial bits. The article shows the results of comparative indicators of serial and experimental bits when their processing on the bench. It was found that experimental bits were more efficient than the serial ones. The estimation of the performance laboratory tests results was confirmed by tests on the bench under pilot conditions of the rolling cutter bits drilling, equipped on the bit gage surface by the compound built-up layer.

scholarly journals Second harmonic generation imaging of fascia within thick tissue block

2007 ◽  
Vol 15 (12) ◽  
pp. 7296 ◽  
Author(s):  
Christian P. Pfeffer ◽  
Bjorn R. Olsen ◽  
François Légaré
Keyword(s):  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Second Harmonic ◽  
Tissue Block ◽  
Second Harmonic Generation Imaging

Paraffin tissue block radiography: adjunct to breast specimen radiography.

Radiology ◽  
1989 ◽  
Vol 173 (3) ◽  
pp. 695-696 ◽  
Author(s):  
M Rebner ◽  
M A Helvie ◽  
D R Pennes ◽  
H A Oberman ◽  
D M Ikeda ◽  
...  
Keyword(s):  
Tissue Block ◽  
Specimen Radiography ◽  
Paraffin Tissue

Inverse Calculation of Flame Impingement Heat Transfer

2006 ◽  
Author(s):  
Ikram Ahmed ◽  
Ildar Sabirov
Keyword(s):  
Heat Transfer ◽  
Radial Distance ◽  
Infrared Camera ◽  
Jet Impingement ◽  
Plasma Jet ◽  
Impingement Heat Transfer ◽  
Direct Measurements ◽  
Polynomial Series ◽  
Gas Flame ◽  
Volume Method

Inverse calculations are presented here for the estimation of heat transfer from an impinging flame on a flat surface. This work is a preliminary exercise for estimating heat transfer from an impinging plasma jet, where direct measurements can be very difficult and costly, and the correlations based on air or water jet impingement measurements may not be applicable because of the very high temperature (and property) gradients. As the gas flame impinges on an initially cold flat plate, the temperature evolution on the backside is recorded using an infrared camera. The time–temperature data thus obtained are then compared with those predicted by a finite volume method based code. The code uses a polynomial series for estimating the convection coefficient, which varies with radial distance. The coefficients of this polynomial are treated as a set of parameters to be estimated through the Levenberg-Marquardt approach. The results obtained so far indicate that it may be possible to use such an approach for estimating heat transfer from a plasma jet.

Corrosion Resistance of VC–FeCr and VC–FeCrСо Coatings Obtained by Supersonic Gas-Flame Spraying

2019 ◽  
Vol 54 (4) ◽  
pp. 535-541 ◽  
Author(s):  
М. М. Student ◽  
H. V. Pokhmurs’ka ◽  
Kh. R. Zadorozhna ◽  
H. H. Veselivs’ka ◽  
V. М. Hvozdets’kyi ◽  
...  
Keyword(s):  
Corrosion Resistance ◽  
Flame Spraying ◽  
Gas Flame
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