A New Approach to the Demonstration of Oxidase-Localization Using Tannic Acid Or Catechin

Author(s):  
V. Mizuhira ◽  
Y. Futaesaku

Previously we reported that tannic acid is a very effective fixative for proteins including polypeptides. Especially, in the cross section of microtubules, thirteen submits in A-tubule and eleven in B-tubule could be observed very clearly. An elastic fiber could be demonstrated very clearly, as an electron opaque, homogeneous fiber. However, tannic acid did not penetrate into the deep portion of the tissue-block. So we tried Catechin. This shows almost the same chemical natures as that of proteins, as tannic acid. Moreover, we thought that catechin should have two active-reaction sites, one is phenol,and the other is catechole. Catechole site should react with osmium, to make Os- black. Phenol-site should react with peroxidase existing perhydroxide.

Rodriguésia ◽  
2021 ◽  
Vol 72 ◽  
Author(s):  
Jaqueline Priscila Bispo de Almeida Cardoso Marcondes ◽  
Rafael Trevisan ◽  
Maria Cecilia de Chiara Moço ◽  
Cleusa Bona

Abstract Eleocharis (Cyperaceae) includes more than 300 species of perennial or annual herbs, frequently found on poorly drained soils. The species are morphologicaly similar, and the taxonomy is difficult because their vegetative and reproductive structures are very reduced. Previous study on the stem architecture in the subgenus Limnochloa showed that anatomical features help in the interpretation of the evolution, taxonomy and ecological aspects of the group. Our objectives were to add new characters from the stem (= culm) structure, to explore the characters in a greater number of Eleocharis species, representatives of the other subgenera, and add these data in a new phylogenetic analysis with molecular data. The study covered 68 species obtained from herbaria and fixed material. In addition to the stem architecture, the internal organization of the tissues, the cross-section format, the presence or absence of stretched cells in aerenchyma air gaps and the plant size were included in the morphological analysis. Our data confirm that spongy aerenchyma pattern is the ancestral condition while the mixed and septate patterns occurred independent and punctually. Only the cross-section format was variable among specimens while the other characters were uniform and relevant for taxonomic use.


1876 ◽  
Vol 24 (164-170) ◽  
pp. 1-32

When any portion of a conducting-wire which joins the two poles of a voltaic battei'y is increased in size, the resistance of the circuit is diminished and a greater current flows through the wire ; and as any increase in the cross section of any portion of the wire causes a corre­sponding increase in the current, it is clear that there is no part of the conducting-wire through which some portion of the current does not flow from the one pole to the other; and the outer boundary of such a wire will be a line of flow. A line of flow is a line drawn in a conductor which indicates at every point of it the direction of the current at that point. A portion of the conductor completely enclosed by lines of flow may be termed a tube of flow.


1992 ◽  
Vol 02 (04) ◽  
pp. 433-440 ◽  
Author(s):  
MASAE YUKAWA ◽  
T. KOBAYASHI ◽  
Y. QIU ◽  
N. KAMEDA ◽  
Y. ITO ◽  
...  

The distribution of elements in human spinal cord was investigated on unfixed frozen cord material using PIXE technique. Distribution of Cu, Zn and Fe were not uniform in the cross section of the spinal cord and concentrations of these elements were higher in the anterior gray horn than in the other areas, while K and Cl distributed uniformly. The content of K changed along the spinsl cord from the cervical to the lumbar level. These findings are discussed in relation to current understanding of the physiology of the spinal cord.


1996 ◽  
Vol 74 (7-8) ◽  
pp. 401-406 ◽  
Author(s):  
E. A. G. Armour

A key process in the muon-catalysed fusion cycle is a low-energy collision of a tμ atom with a DA molecule, where A is H, D, or T, which leads at appropriate incident energies, to the formation of a resonant complex containing dtμ. In this paper, methods of calculating the resonant formation rate of dtμ are discussed. A description is given of a new approach that makes use of coupled equations for the rearrangement scattering process and elements of Feshbach's theory of resonances to obtain an expression for the cross section for resonant dtμ formation. The insights gained from this approach are discussed.


2021 ◽  
Vol 93 (1) ◽  
pp. 26-37
Author(s):  
O.V. RADAYKIN ◽  
◽  
L.S. SABITOV ◽  
L.SH. AKHTYAMOVA ◽  
T.P. ARAKCHEEV ◽  
...  

In SP 63.13330 the general calculation case for the diagram method is oblique off-center compression, which takes into account only three components of internal force factors in the cross section: the longitudinal force-Nz – and two bending moments relative to the corresponding axes – Mx and My. The other three components-the QX and Qy transfer forces and the MZ torque – are left out of consideration. In addition, for this case, the search in the available literature, including the founders of the diagram method, for the output of calculation formulas was not successful – in all sources they are given in ready-made form without evidence. This article is intended to try to fill in these gaps. For this purpose, based on the expressions for rod displacements that are generally accepted in mechanics, in particular on the Mora integral of displacements, the resolving expressions of the diagram method are obtained in the most general form.


1954 ◽  
Vol 58 (524) ◽  
pp. 541-546 ◽  
Author(s):  
B. D. Henshall

In recent years interest has been revived in an apparatus which was first developed more than fifty years ago. This apparatus, which is known today as a shock tube, is a very useful research tool, the versatility of which is not generally appreciated in this country. In this paper the author attempts to give a clear physical description of the flow in a shock tube and a review of the problems which may be investigated by means of this apparatus.Basically, a shock tube consists of a simple fabricated duct which may be closed or open at one end, and closed at the other end. A diaphragm divides this duct into two compartments which initially contain gases at different pressures. In a conventional shock tube the cross-section is constant, the ends are closed, and air is used on both sides of the diaphragm (Fig. 1).


Fire Research ◽  
2019 ◽  
Vol 3 (1) ◽  
Author(s):  
António Correia ◽  
Paula Lopes ◽  
João Rodrigues ◽  
José Correia

The fire resistance of a steel column is highly affected by the contact between the columns and the walls, leading in general to a favorable effect due to the reduction of temperatures. However, it leads to the Thermal Bowing effect, which is not more than a differential heating in the steel cross sections, causing an inversion of bending moments and an inversion of the deflections of the column. Thus, it is necessary to accurately assess the evolution of the temperature field in the cross section of the steel elements in contact with walls. In Eurocode 3 part 1-2, the structural design of steel elements in fire situation is performed with expressions for the calculation of the section factor of steel profiles, but different cases of positioning the columns and the surrounding walls could be considered as causing extremely high thermal gradients. In this paper, a new approach for the calculation of section factors for cases not included in table 4.2 of Eurocode 3, part 1- 2 are presented. This was achieved using numerical models with finite element modelling with the ABAQUS program, varying the cross-section of the columns, orientation of the web in relation to the walls, and the position and thickness of the walls, to achieve the desired section factors.


2008 ◽  
Vol 06 (01) ◽  
pp. 11-22 ◽  
Author(s):  
GEORGES GRISO

In this paper, we study the asymptotic behavior of a structure made of curved rods of thickness 2δ when δ tends to 0. This study is carried on within the frame of linear elasticity by using the unfolding method. It is based on several decompositions of the structure displacements and on the passing to the limit in fixed domains. We show that any displacement of a structure is the sum of an elementary rods-structure displacement (e.r.s.d.) concerning the rods' cross sections and a residual one related to the deformation of the cross section. The e.r.s.d. coincides with rigid body displacements in the junctions. Any e.r.s.d. is given by two functions belonging to [Formula: see text] where [Formula: see text] is the skeleton structure (i.e. the set of rods with middle lines). One of this function [Formula: see text] is the skeleton displacement, the other [Formula: see text] gives the cross section rotation. We show that [Formula: see text] is the sum of an extensional displacement and an inextensional one. We establish a priori estimates and then, we characterize the unfolded limits of the rods-structure displacements. Eventually, we pass to the limit in the linearized elasticity system and using all results in [6], where on one hand, we obtain a variational problem that is satisfied by the limit extensional displacement, and on the other hand, a variational problem coupling the limit of inextensional displacement and the limit of the rod torsion angles.


1966 ◽  
Vol 21 (9) ◽  
pp. 1321-1327 ◽  
Author(s):  
Elmar Zeitler ◽  
Haakon Olsen

Results of calculations of cross sections for elastic electron and positron scattering are given in angular steps of 15 degrees for elements Z=6, 13, 29, 50, 82, and 92 and energies T=0.2, 0.4, 0.7, 1, 2, 4, and 10 MeV. The calculation is based on the separability of the cross section into two factors, one describing screening and the other, spin and relativistic effects. The first factor is obtained by the MOLIÈRE approximation 8. The second factor is taken from a paper by DOGGETT and SPENCER 5. Different screening potentials for Z=29 were applied.


2018 ◽  
Vol 7 (3.34) ◽  
pp. 339
Author(s):  
Nagasankar P ◽  
Tamilselvi P ◽  
Babu T V B ◽  
Jasim Salahudin S ◽  
Aravindhan S ◽  
...  

This paper is a description of an alternative fixture for the edge preparation of elbows and pipes before welding. The welding will not penetrate the cross section of the pipe if the thickness of the pipes is more than eight millimeter, so edge preparation is done before welding. Fixtures are used to hold the pipes during the process of edge preparation. This paper deals with two major issues from the design of previous fixtures.Requirement of manual handling of the part.Precise rotations are not possible. The fixtures being used currently require more setting up time and they require manual handling to machine the other side. This paper describes a fixture which can reduce manual handling and be able to rotate the elbow either manually or using a motor.  


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