Electron Microscopic and Optical Diffraction Analyses of Crystalline Intranuclear Inclusions in Human Osteosarcoma Cells

1981 ◽  
Vol 39 ◽  
pp. 436-437
Author(s):  
Gonpachiro Yasuzumi
Keyword(s):  
Electron Micrographs ◽  
Periodic Structures ◽  
Bragg Reflection ◽  
Optical Diffraction ◽  
Intranuclear Inclusions ◽  
Osteosarcoma Cells ◽  
Electron Microscopic ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cells ◽  
Diffraction Patterns

The fine structure of the crystalline intranuclear inclusions in the human osteosarcoma cells was studied by using a goniometer which tilted the specimen at angles of ±30° and ±40°. The results appear in the series of micrographs showing Fig. 1. At the point by the arrow, a helical structure is visible in two filaments. Optical diffrection patterns of selected areas of each negative electron micrograph film were taken by using a helium-neon laser as a source.A tentative model of the structure can be based on optical diffraction techniques applied to electron micrographs taken at different tilt angles. The diffraction pattern taken from image No. 0 in Fig. 1 is depicted in Fig. 2. Diffraction patterns from Nos. 3, 4 and 8 are shown in Figs. 3, 4 and 5. The contrast of some periodic structures can be eliminated in the image (extinction effect due to Bragg reflection of electron waves) by the interference of scattered waves from constituent elements. Hence it is rather important to interpret the electron micrographs and their optical diffraction patterns by considering the extinction effect of the image contrast.

scholarly journals Electron Microscopic Cytochemical Analysis of Paracystalline Intranuclear Inclusions in Human Osteosarcoma Cells

1982 ◽  
Vol 58 (4-6) ◽  
pp. 369-381
Author(s):  
FUMIOKI YASUZUMI ◽  
HIRONORI SAKAMOTO ◽  
YOSHIO MII ◽  
NORIKO YABUMOTO ◽  
GONPACHIRO YASUZUMI
Keyword(s):  
Intranuclear Inclusions ◽  
Osteosarcoma Cells ◽  
Electron Microscopic ◽  
Human Osteosarcoma ◽  
Cytochemical Analysis ◽  
Human Osteosarcoma Cells

scholarly journals An electron microscopic and optical diffraction analysis of the structure of Limulus telson muscle thick filaments.

1982 ◽  
Vol 92 (2) ◽  
pp. 443-451 ◽  
Author(s):  
R W Kensler ◽  
R J Levine
Keyword(s):  
Electron Microscopy ◽  
Diffraction Analysis ◽  
Electron Micrographs ◽  
Optical Diffraction ◽  
X Ray Diffraction ◽  
Electron Microscopic ◽  
X Ray ◽  
Thick Filaments ◽  
Diffraction Patterns ◽  
Cross Bridges

Long, thick filaments (greater than 4.0 micrometer) rapidly and gently isolated from fresh, unstimulated Limulus muscle by an improved procedure have been examined by electron microscopy and optical diffraction. Images of negatively stained filaments appear highly periodic with a well-preserved myosin cross-bridge array. Optical diffraction patterns of the electron micrographs show a wealth of detail and are consistent with a myosin helical repeat of 43.8 nm, similar to that observed by x-ray diffraction. Analysis of the optical diffraction patterns, in conjunction with the appearance in electron micrographs of the filaments, supports a model for the filament in which the myosin cross-bridges are arranged on a four-stranded helix, with 12 cross-bridges per turn or each helix, thus giving an axial repeat every third level of cross-bridges (43.8 nm).

Facile designed poly(lactic-co-glycolic acid)-containing nano-platform carried dual drug system to improve progressive combination cancer therapy against human osteosarcoma cells

2020 ◽  
Vol 10 (10) ◽  
pp. 1599-1606
Author(s):  
Pengyun Wang ◽  
Lei Wei ◽  
Shuye Yang ◽  
Xuebo Dong ◽  
Zhen Wang ◽  
...  
Keyword(s):  
Glycolic Acid ◽  
Spectroscopic Techniques ◽  
Synergistic Activity ◽  
Osteosarcoma Cells ◽  
Electron Microscopic ◽  
Transmission Microscopy ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cells ◽  
Dual Drug

We have designed a methodology to achieve treatment-actuated modifications in a tumor microenvironment by utilizing synergistic activity between two potential anticancer drugs. Dual drug delivery of Sorafenib (Sf) along with Cisplatin (Pt) exhibits a great anti-cancer potential, as Sf enhances the effect of Pt treatment of human osteosarcoma cells by providing microenvironment stability. However, encapsulation of Sf and Pt obsessed by poly(lactic-co-glycolic acid (PLGA)-based nanoparticles (PLGA-NPs) is incompetent owing to unsuitability between the binary free Sf and Pt moieties and the polymeric system. Now, we display that Pt can be prepared by hydrophobic covering of the drug centers with dioleoylphosphatidic acid (DOPA). The DOPA-covered Pt centers can be co-encapsulated in PLGA NPs alongside Sf to stimulate excellent anticancer property. The occurrence of the Pt centers suggestively enhanced the encapsulations of Sf into PLGA-NPs. Formation of the nanocomposite (Pt-Sf@PLGA-NPs) was confirmed by FTIR and X-ray spectroscopic techniques. Further, the morphology of Pt-Sf@PLGA-NPs and nanoparticle size was examined by scanning electron microscopic and transmission microscopy, respectively. Furthermore, Pt-Sf@PLGA-NPs induced significant apoptosis in human osteosarcoma MG-63 and U2OS cancer cells in vitro. The results suggest that Pt-Sf@PLGA-NPs are one of the promising human osteosarcoma cancer therapeutic candidates worthy of further investigations.

Light Optical Diffraction Studies of Macromolecular Ultrastructure

1970 ◽  
Vol 28 ◽  
pp. 258-259
Author(s):  
Glen B. Haydon
Keyword(s):  
High Resolution ◽  
Diffraction Analysis ◽  
Diffraction Pattern ◽  
Electron Micrographs ◽  
Optical Diffraction ◽  
Electron Micrograph ◽  
Its Analysis ◽  
Resolution Electron ◽  
Diffraction Patterns

Analysis of light optical diffraction patterns produced by electron micrographs can easily lead to much nonsense. Such diffraction patterns are referred to as optical transforms and are compared with transforms produced by a variety of mathematical manipulations. In the use of light optical diffraction patterns to study periodicities in macromolecular ultrastructures, a number of potential pitfalls have been rediscovered. The limitations apply to the formation of the electron micrograph as well as its analysis.(1) The high resolution electron micrograph is itself a complex diffraction pattern resulting from the specimen, its stain, and its supporting substrate. Cowley and Moodie (Proc. Phys. Soc. B, LXX 497, 1957) demonstrated changing image patterns with changes in focus. Similar defocus images have been subjected to further light optical diffraction analysis.

Antiproliferative properties of oleuropein in human osteosarcoma cells

2016 ◽  
Author(s):  
Jose M Moran ◽  
Olga Leal-Hernandez ◽  
Maria L Canal-Macias ◽  
Jesus M Lavado-Garcia ◽  
Raul Roncero-Martin ◽  
...  
Keyword(s):  
Osteosarcoma Cells ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cells

Copper(II) Complexes with Saccharinate and Glutamine as Antitumor Agents: Cytoand Genotoxicity in Human Osteosarcoma Cells

2017 ◽  
Vol 17 (3) ◽  
pp. 424-433 ◽  
Author(s):  
Juan Cadavid-Vargas ◽  
Ignacio Leon ◽  
Susana Etcheverry ◽  
Eduardo Santi ◽  
Maria Torre ◽  
...  
Keyword(s):  
Antitumor Agents ◽  
Osteosarcoma Cells ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cells

scholarly journals Assessment of the Nutraceutical Effects of Oleuropein and the Cytotoxic Effects of Adriamycin, When Administered Alone and in Combination, in MG-63 Human Osteosarcoma Cells

Nutrients ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 354
Author(s):  
Katerina Gioti ◽  
Anastasia Papachristodoulou ◽  
Dimitra Benaki ◽  
Nektarios Aligiannis ◽  
Alexios-Leandros Skaltsounis ◽  
...  
Keyword(s):  
Chemotherapeutic Agent ◽  
Molecular Techniques ◽  
Osteosarcoma Cells ◽  
Elisa Method ◽  
Chain Reaction ◽  
Cytotoxic Effects ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cells ◽  
Anti Cancer ◽  
Polymerase Chain

Oleuropein (OLEU) is the most distinguished phenolic compound found in olive fruit and the leaves of Olea europaea L., with several pharmacological properties, including anti-cancer actions. Adriamycin (ADR) is an anthracycline widely used as a chemotherapeutic agent, although it presents significant side effects. The aim of the present study was to investigate the effect of oleuropein alone (20 μg/mL) and in co-treatment with ADR (50 nM), in MG-63 human osteosarcoma cells. Therefore, cellular and molecular techniques, such as MTT assay, flow cytometry, real-time Polymerase Chain Reaction (PCR), western blot and Elisa method, as well as Nuclear Magnetic Resonance (NMR) spectroscopy, were applied to unveil changes in the signal transduction pathways involved in osteosarcoma cells survival. The observed alterations in gene, protein and metabolite levels denote that OLEU not only inhibits MG-63 cells proliferation and potentiates ADR’s cytotoxicity, but also exerts its action, at least in part, through the induction of autophagy.

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