Cone-beam 3D image reconstruction in x-ray microtomography

Author(s):  
G. Wang ◽  
P. C. Cheng ◽  
T. H. Lin ◽  
D. M. Shinozaki ◽  
H. Kim

An X-ray shadow projection microscope system using a scannable point source of X-rays is under development at AMIL-ARTS, SUNY at Buffalo, USA. The point source is generated by a focussed electron beam, which can be steered electromagnetically in a plane perpendicular to the optical axis of the microscope. A specimen is mounted on a rotatable mechanical stage for microtomography. Considering the hardware characteristics of this system and the limitations of current cone-beam reconstruction algorithms, a generalized Feldkamp’s cone-beam image reconstruction algorithm has been developed at our laboratories. In our cone-beam reconstruction, there are mainly two kinds of scanning scanning modes: planar and helix-like. A planar scanning locus is used to handle spherical or plate-like specimens. A typical case of planar scanning loci is a circle, which is used in Feldkamp’s cone-beam reconstruction. A helix-like scanning locus is used to deal with rod-shaped specimens. Without loss of generality, a locus turn of the X-ray source can be defined in cylindrical coordinates by the following equation:

1999 ◽  
Vol 5 (S2) ◽  
pp. 940-941
Author(s):  
Shih Ang ◽  
Wang Ge ◽  
Cheng Ping-Chin

Due to the penetration ability and absorption contrast mechanism, cone-beam X-ray microtomography is a powerful tool in studying 3D microstructures in opaque specimens. In contrast to the conventional parallel and fan-beam geometry, the cone-beam tomography set up is highly desirable for faster data acquisition, build-in magnification, better radiation utilization and easier hardware implementation. However, the major draw back of the cone-beam reconstruction is its computational complexity. In an effort to maximize the reconstruction speed, we have developed a generalized Feldkamp cone-beam reconstruction algorithm to optimize the reconstruction process. We report here the use of curved voxels in a cylindrical coordinate system and mapping tables to further improve the reconstruction efficiency.The generalized Feldkamp cone-beam image reconstruction algorithm is reformulated utilizing mapping table in the discrete domain as: , where .


10.14311/1312 ◽  
2011 ◽  
Vol 51 (1) ◽  
Author(s):  
V. Grinberg ◽  
I. Kreykenbohm ◽  
F. Fürst ◽  
J. Wilms ◽  
K. Pottschmidt ◽  
...  

INTEGRAL is one of the few instruments capable of detecting X-rays above 20 keV. It is therefore in principle well suited for studying X-ray variability in this regime. Because INTEGRAL uses coded mask instruments for imaging, the reconstruction of light curves of X-ray sources is highly non-trivial. We present results from a comparison of two commonly employed algorithms, which primarily measure flux from mask deconvolution (ii_lc_extract) and from calculating the pixel illuminated fraction (ii_light). Both methods agree well for timescales above about 10 s, the highest time resolution for which image reconstruction is possible. For higher time resolution, ii light produces meaningful results, although the overall variance of the lightcurves is not preserved.


1992 ◽  
Author(s):  
Ge Wang ◽  
T. H. Lin ◽  
Ping C. Cheng ◽  
D. M. Shinozaki ◽  
Hyo-Gun Kim

Author(s):  
D. A. Carpenter ◽  
Ning Gao ◽  
G. J. Havrilla

A monolithic, polycapillary, x-ray optic was adapted to a laboratory-based x-ray microprobe to evaluate the potential of the optic for x-ray micro fluorescence analysis. The polycapillary was capable of collecting x-rays over a 6 degree angle from a point source and focusing them to a spot approximately 40 µm diameter. The high intensities expected from this capillary should be useful for determining and mapping minor to trace elements in materials. Fig. 1 shows a sketch of the capillary with important dimensions.The microprobe had previously been used with straight and with tapered monocapillaries. Alignment of the monocapillaries with the focal spot was accomplished by electromagnetically scanning the focal spot over the beveled anode. With the polycapillary it was also necessary to manually adjust the distance between the focal spot and the polycapillary.The focal distance and focal spot diameter of the polycapillary were determined from a series of edge scans.


Author(s):  
Santosh Bhattacharyya

Three dimensional microscopic structures play an important role in the understanding of various biological and physiological phenomena. Structural details of neurons, such as the density, caliber and volumes of dendrites, are important in understanding physiological and pathological functioning of nervous systems. Even so, many of the widely used stains in biology and neurophysiology are absorbing stains, such as horseradish peroxidase (HRP), and yet most of the iterative, constrained 3D optical image reconstruction research has concentrated on fluorescence microscopy. It is clear that iterative, constrained 3D image reconstruction methodologies are needed for transmitted light brightfield (TLB) imaging as well. One of the difficulties in doing so, in the past, has been in determining the point spread function of the system.We have been developing several variations of iterative, constrained image reconstruction algorithms for TLB imaging. Some of our early testing with one of them was reported previously. These algorithms are based on a linearized model of TLB imaging.


2017 ◽  
Vol 2017 ◽  
pp. 1-10
Author(s):  
Hsuan-Ming Huang ◽  
Ing-Tsung Hsiao

Background and Objective. Over the past decade, image quality in low-dose computed tomography has been greatly improved by various compressive sensing- (CS-) based reconstruction methods. However, these methods have some disadvantages including high computational cost and slow convergence rate. Many different speed-up techniques for CS-based reconstruction algorithms have been developed. The purpose of this paper is to propose a fast reconstruction framework that combines a CS-based reconstruction algorithm with several speed-up techniques.Methods. First, total difference minimization (TDM) was implemented using the soft-threshold filtering (STF). Second, we combined TDM-STF with the ordered subsets transmission (OSTR) algorithm for accelerating the convergence. To further speed up the convergence of the proposed method, we applied the power factor and the fast iterative shrinkage thresholding algorithm to OSTR and TDM-STF, respectively.Results. Results obtained from simulation and phantom studies showed that many speed-up techniques could be combined to greatly improve the convergence speed of a CS-based reconstruction algorithm. More importantly, the increased computation time (≤10%) was minor as compared to the acceleration provided by the proposed method.Conclusions. In this paper, we have presented a CS-based reconstruction framework that combines several acceleration techniques. Both simulation and phantom studies provide evidence that the proposed method has the potential to satisfy the requirement of fast image reconstruction in practical CT.


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