Site of hemocyanin synthesis in the terrestrial isopod, Armadillidiom vulgare

1989 ◽  
Vol 47 ◽  
pp. 970-971
Author(s):  
L. Faso ◽  
E. Rappa ◽  
G. Vernon ◽  
R. Witkus
Keyword(s):  
Electron Microscope ◽  
Transmission Electron Microscope ◽  
Blood Cells ◽  
Osmium Tetroxide ◽  
Oxygen Binding ◽  
Sodium Cacodylate Buffer ◽  
Sodium Cacodylate ◽  
Astacus Astacus ◽  
Transmission Electron ◽  
Cacodylate Buffer

Although hemocyanin, an oxygen binding protein, is found freely dissolved in the hemolymph of isopods its site of synthesis is still unknown.Circulating blood cel Is such as granular hemocytes have been implicated in hemocyanin synthesis in a number of arthropods including Astacus astacus and Homarus vulgaris. Circulating blood cells of Armadillidium vulgare were examined using a transmission electron microscope (TEM) for evidence of hemocyanin synthesis.For each experiment hemolymph was collected from twenty adult A. vulgare and fixed for 1 hour in 200 uL of 3.5% glutaraldehyde in 0.1M sodium cacodylate buffer pH 7.4 with 0.05% calcium chloride added. Hemolymph was then centrifuged at 3000 rpm in an IEC-DPR-6000 centrifuge for 15 minutes at 15 degrees centigrade. The supernatant was removed, and the resulting pellet was washed with three changes of sodium cacodylate buffer. Postfixation of the pellet was done in 1% osmium tetroxide for 1 hour.

Bacterioids in the Rectal Organ of the Lantern Dug Pyrops Candelaria Linn (Homoptera: Fulgoridae)

1996 ◽  
Vol 54 ◽  
pp. 806-807
Author(s):  
W.W.K. Cheung ◽  
J.B. Wang
Keyword(s):  
Fine Structure ◽  
Electron Microscope ◽  
Osmium Tetroxide ◽  
Uranyl Acetate ◽  
Sodium Cacodylate Buffer ◽  
Lead Citrate ◽  
Sodium Cacodylate ◽  
Special Pair ◽  
Adult Females ◽  
Cacodylate Buffer

The lantern bug harbours three symbionts, namely a, x and i in its body. These microorganisms are supposed to be transmitted transovarially to the future progeny. The x-symbionts are found in a special pair of organs called the x-organ which bulges to form a rectal organ in adult females. The purpose of this study is to investigate into the fine structure of the x-symbionts. This will serve as a basis for understanding the interactions of this microorganism with its host.The rectum of the lantern bug Pyrops candelaria Linn, was dissected out in buffered insect saline and fixed in 2.5% glutaradehyde in 0.1M sodium cacodylate buffer (pH 7.2) for 1 hr. The rectal organ was subsequently post-fixed in 1% osmium tetroxide (pH 7.2) and dehydrated in alcohol/acetone series. These were blocked in Spurr resin and cut with a Reichert Ultratome. Sections were stained with uranyl acetate and lead citrate and examined with a JEOL JEM-1200EX electron microscope. Thick sections (1 μn) were stained with 1% toluidine blue and examined under a Nikon Optiphot light microscope.

Oligodendrocytes in Developing Hameter Cerebral Cortex

1976 ◽  
Vol 34 ◽  
pp. 126-127
Author(s):  
MB. Tank Buschmann
Keyword(s):  
Cerebral Cortex ◽  
Optic Nerve ◽  
Frontal Cortex ◽  
Osmium Tetroxide ◽  
Sodium Cacodylate Buffer ◽  
Sodium Cacodylate ◽  
Tissue Samples ◽  
Cacodylate Buffer ◽  
Layer V ◽  
Adult Hamster

Development of oligodendrocytes in rat corpus callosum was described as a sequential change in cytoplasmic density which progressed from light to medium to dark (1). In rat optic nerve, changes in cytoplasmic density were not observed, but significant changes in morphology occurred just prior to and during myelination (2). In our study, the ultrastructural development of oligodendrocytes was studied in newborn, 5-, 10-, 15-, 20-day and adult frontal cortex of the golden hamster (Mesocricetus auratus).Young and adult hamster brains were perfused with paraformaldehyde-glutaraldehyde in sodium cacodylate buffer at pH 7.3 according to the method of Peters (3). Tissue samples of layer V of the frontal cortex were post-fixed in 2% osmium tetroxide, dehydrated in acetone and embedded in Epon-Araldite resin.

Nonspecific structures seen in diagnostic muscle biopsies

1987 ◽  
Vol 45 ◽  
pp. 846-847
Author(s):  
R. C. Caughey ◽  
U. P. Kalyan-Raman
Keyword(s):  
Electron Microscope ◽  
Transmission Electron Microscope ◽  
Osmium Tetroxide ◽  
Clinical History ◽  
Uranyl Acetate ◽  
Ethanol Dehydration ◽  
Tubular Aggregates ◽  
En Bloc ◽  
Transmission Electron ◽  
Muscle Biopsies

In a period of two years we have analyzed 50 muscle biopsies using the transmission electron microscope. Six nonspecific structures consisting of filamentous bodies, tubular aggregates, paracrystalline mitochondrial inclusions, honeycomb arrays, concentric laminated bodies, and finger print profiles were observed in 47 of 50 cases. In order to know the significance of these structures in muscle biopsies, we correlated their occurrence with their clinical history, histological findings, and histochemistry.The biopsies were initially fixed in 2.5% glutaraldehyde (pH. 7.5, 500 mOsm), then randomly minced and post fixed in 1% osmium tetroxide. All biopsies were processed with and without uranyl acetate en bloc staining in Walpole's buffer before ethanol dehydration. They were embedded in Epon 812 epoxy resin, sectioned, and stained with uranyl acetate and lead citrate before evaluation with a JEOL, JEM 100 C Transmission Electron Microscope. All grid squares of six different blocks were scanned to evaluate the ultra-structural pathology.

Fine Structural Studies on the Scolex of Hymenolepis diminuta (Cestoda)

1974 ◽  
Vol 32 ◽  
pp. 184-185
Author(s):  
R. D. Specian ◽  
V. F. Allison ◽  
J. E. Ubelaker
Keyword(s):  
Electron Microscope ◽  
Transmission Electron Microscope ◽  
Osmium Tetroxide ◽  
Neurosecretory Cells ◽  
The Body ◽  
Hymenolepis Diminuta ◽  
Structural Studies ◽  
Major Area ◽  
Laboratory Rats ◽  
Transmission Electron

Although the scolex is the major area of contact between a cestode and its host, most fine structural studies have concentrated on the rest of the body. Davey and Breckenridge postulated, from histochemical data, that neurosecretory cells were present in the scolex of Hymenolepis diminuta. Such cells have been suggested to function in affecting growth and maturation of the strobila. Since a previous study by Rothman failed to determine such cells, the present study was undertaken.Specimens were collected from previously infected laboratory rats, fixed in paraformaldehyde, post-fixed in osmium tetroxide and embedded in Maraglas. Sections were examined on the Hitachi HU11B-2 transmission electron microscope.

A polymer designed to optimize antigen localization in immunoelectron microscopy

1990 ◽  
Vol 48 (3) ◽  
pp. 790-791
Author(s):  
J.B. Olesen ◽  
C.A. Heckman ◽  
A. Lukinius
Keyword(s):  
Electron Microscopy ◽  
Electron Microscope ◽  
Red Blood Cells ◽  
Immunoelectron Microscopy ◽  
Transmission Electron Microscope ◽  
Blood Cells ◽  
Room Temperature ◽  
Human Red Blood Cells ◽  
Transmission Electron ◽  
Antigen Localization

We have developed a new polymer for the purpose of retaining the maximal antigenicity of proteins in samples embedded for electron microscopy. HACH is a mixture of hydroxyhexanedial, carbohydrazide and hydrazine that polymerizes at room temperature within 24 h. Tissues embedded in the polymer were thin sectioned and the sections, mounted on formvar films, were viewed with a Zeiss IOC transmission electron microscope. The ultrastructural features of cells in HACH-embedded tissues appeared similar to those previously found in GACH-embedded tissues.The ability to preserve the antigenicity of a protein up to the polymerization stage has been tested in the following manner. Human red blood cells, from either Rh+ or Rh- donors, were first exposed to the HACH prepolymer and then rinsed and exposed to a commercially available anti-D antibody. After removal of the prepolymer, Rh+ red blood cells were clumped by anti-D antibody to the same extent as control, untreated cells (Fig. 1).

scholarly journals In situ Generation of Ruthenium Tetroxide and Osmium Tetroxide for the Physical Sciences and Their Reaction Indicators

2002 ◽  
Vol 10 (5) ◽  
pp. 20-23
Author(s):  
Paul Beauregard
Keyword(s):  
Electron Microscope ◽  
Transmission Electron Microscope ◽  
Osmium Tetroxide ◽  
Physical Sciences ◽  
Thin Sections ◽  
Ruthenium Tetroxide ◽  
Transmission Electron ◽  
In Situ Generation

Recently, there was a suggestion on the MSA listserver about the use of osmium tetroxide (OsO4 and how to handle it. One suggestion was that ampoules be scored, placed in a glass jar, and the ampoule smashed to release the contents. This seemed like a very unsafe way to use osmium tetroxide or ruthenium tetroxide. The purpose of this article is to suggest a way to generate smaller amounts of these compounds in a safer manner than smashing ampoules and wondering about what to do with the unused portion after staining or storing. Another purpose is to discuss a new reaction indicator for mainly osmium tetroxide. The use of a reaction specific indicator was mandatory for judging the level or degree to which staining had proceeded in thin sections for the transmission electron microscope (TEM).

Ultrastructural Localization of Cholesterol in Human Arteriosclerosis

1973 ◽  
Vol 31 ◽  
pp. 400-401
Author(s):  
Karta Sekhri ◽  
Clint Mills ◽  
Henry Younes
Keyword(s):  
Fine Structure ◽  
Electron Microscope ◽  
Free Cholesterol ◽  
Osmium Tetroxide ◽  
Clinical Evidence ◽  
Ultrastructural Localization ◽  
Sodium Cacodylate ◽  
Cacodylate Buffer

Several reports have appeared recently demonstrating a technique for the localization of cholesterol in a variety of tissues. The technique is based on the premise that free cholesterol reacts with digitonin to form cholesterol digitonide which appears as needle-like crystals or spicules under the electron microscope.In order to study the fine structure of the aorta, surgical biopsies from several patients (ranging in age from 45 to 68 years) and with clinical evidence of arteriosclerosis were fixed promptly in Flickinger's fixative (2% formaldehyde, 2.5% glutaraldehyde) containing 0.2% digitonin but buffered with .1M sodium cacodylate. After fixation for 3 hours aortic samples were washed in cacodylate buffer and post fixed in buffered osmium tetroxide for 2 hours & embedded in Epon-Araldite.

Scanning Electron Microscopy of Blaberus Giganteus (L.) Maxillary Palp Sensilla

1976 ◽  
Vol 34 ◽  
pp. 194-195
Author(s):  
P. Dailey
Keyword(s):  
Osmium Tetroxide ◽  
Ventral Surface ◽  
Maxillary Palp ◽  
Sodium Cacodylate Buffer ◽  
Sodium Cacodylate ◽  
Sensilla Basiconica ◽  
Critical Point Drying ◽  
Cacodylate Buffer ◽  
Maxillary Palps ◽  
Scanning Electron

The maxillary palps of B. giganteus are located on the maxillae which lie between the labrum and labium. They are modified appendages used in feeding. Each appendage bears 5 segments, the most distal of which contains the palp organ or sensory pad (Fig. 1). The maxillary palps were fixed in 2.5% glutaraldehyde in 0.05 M sodium cacodylate buffer (pH 7.1) containing .15 M sucrose and post-fixed in 1% osmium tetroxide in buffer. This was followed by dehydration and critical point drying. Cryofractography was performed on some of the palp organs prior to drying (1).The sensory pad is composed of an exocuticular membrane upon which lie numerous sensilla basiconica in an elliptical pattern on the ventral surface. The membrane enables the sensory pad to expand and, in living specimens it appears dome-like due to the pressure exerted on it by the hemolymph.

A Reproducible Selective Stain for Cardiac Sarcoplasmic Reticulum

1974 ◽  
Vol 32 ◽  
pp. 214-215
Author(s):  
R. A. Waugh ◽  
J. R. Sommer
Keyword(s):  
Complex System ◽  
Electron Microscope ◽  
Sarcoplasmic Reticulum ◽  
Transmission Electron Microscope ◽  
Electron Microscopic ◽  
Cardiac Sarcoplasmic Reticulum ◽  
Transmission Electron

Cardiac sarcoplasmic reticulum (SR) is a complex system of intracellular tubules that, due to their small size and juxtaposition to such electron-dense structures as mitochondria and myofibrils, are often inconspicuous in conventionally prepared electron microscopic material. This study reports a method with which the SR is selectively “stained” which facilitates visualizationwith the transmission electron microscope.

Sign in / Sign up

Export Citation Format

Share Document