Evaluation of hippocampus in rhesus monkeys after chronic (1-year) inhalation exposure to marijuana: II. Morphometric methods

Author(s):  
S.W. Wilson ◽  
A.M. Andrews ◽  
A.C. Scallet ◽  
S.F. Ali ◽  
J.R. Bailey ◽  
...  

An innovative method was used to collect data for morphometric analysis of synaptic measurements. Negatives of the CA3 region of the hippocampus were obtained as described by Andrews et al., 1990, and evaluated by computersupported image analysis. Negatives of 4000X and 40,000X magnification were used to measure synaptic density and width, respectively. The image analysis system (IAS) used an IBM-AT compatible computer with a frame grabber (Imaging Research Inc.). This particular system can measure either straight line distance (rubber line) or curved two point distances (draw line).For synaptic density, the IAS was calibrated in angstroms at a magnification of 4000X. A log file was created for each negative in which the measurement for each synapse was stored. The negative was placed on a transparency with grids 9 mm square to ensure that the entire negative was scanned. The image was displayed on a multicsync monitor using a high resolution camera (Applied Intelligent Systems Inc.). When a synapse came into view, the image was digitized on the monitor (Fig. 1 A and B) and stored in digital form.

Author(s):  
D.S. DeMiglio

Much progress has been made in recent years towards the development of closed-loop foundry sand reclamation systems. However, virtually all work to date has determined the effectiveness of these systems to remove surface clay and metal oxide scales by a qualitative inspection of a representative sampling of sand particles. In this investigation, particles from a series of foundry sands were sized and chemically classified by a Lemont image analysis system (which was interfaced with an SEM and an X-ray energy dispersive spectrometer) in order to statistically document the effectiveness of a reclamation system developed by The Pangborn Company - a subsidiary of SOHIO.The following samples were submitted: unreclaimed sand; calcined sand; calcined & mechanically scrubbed sand and unused sand. Prior to analysis, each sample was sprinkled onto a carbon mount and coated with an evaporated film of carbon. A backscattered electron photomicrograph of a field of scale-covered particles is shown in Figure 1. Due to a large atomic number difference between sand particles and the carbon mount, the backscattered electron signal was used for image analysis since it had a uniform contrast over the shape of each particle.


2020 ◽  
pp. 49-52
Author(s):  
Trine Aabo Andersen

A new fast measuring method for process optimization of sucrose crystallization using image analysis based on high quality images and algorithms is introduced. With the mobile, non-invasive at-line system all steps of the sucrose crystallization can be measured to determine the crystal size distribution. The image analysis system is easy to operate and is as well an efficient laboratory solution with user-friendly and customized software. In comparison to sieve analysis, image analyses performed with the ParticleTech Solution have been proven to be reliable.


1986 ◽  
Vol 21 (1) ◽  
pp. 130-140 ◽  
Author(s):  
Da-hong Li ◽  
J. J. Ganczarczyk

Abstract The computerized image analysis system has been successfully used for determination and statistical processing of the following geometric characteristics of activated sludge flocs: longest dimension, breadth, equivalent diameter, cross-sectional area, perimeter, elongation, and circularity. These parameters could be effectively and precisely determined by the system applied. In addition, the studied method, as compared to direct microscope observation and photography floc-sizing methods, was found to be more accurate, less time-consuming, and less dependent on the investigators.


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