Routine Digital Processing of Microscope Images

Author(s):  
E. Wisse ◽  
A. Geerts ◽  
R.B. De Zanger

The slowscan and TV signal of the Philips SEM 505 and the signal of a TV camera attached to a Leitz fluorescent microscope, were digitized by the data acquisition processor of a Masscomp 5520S computer, which is based on a 16.7 MHz 68020 CPU with 10 Mb RAM memory, a graphics processor with two frame buffers for images with 8 bit / 256 grey values, a high definition (HD) monitor (910 × 1150), two hard disks (70 and 663 Mb) and a 60 Mb tape drive. The system is equipped with Imaging Technology video digitizing boards: analog I/O, an ALU, and two memory mapped frame buffers for TV images of the IP 512 series. The Masscomp computer has an ethernet connection to other computers, such as a Vax PDP 11/785, and a Sun 368i with a 327 Mb hard disk and a SCSI interface to an Exabyte 2.3 Gb helical scan tape drive. The operating system for these computers is based on different versions of Unix, such as RTU 4.1 (including NFS) on the acquisition computer, bsd 4.3 for the Vax, and Sun OS 4.0.1 for the Sun (with NFS).

Author(s):  
David Svoboda ◽  
Marek Kašík ◽  
Martin Maška ◽  
Jan Hubený ◽  
Stanislav Stejskal ◽  
...  

2005 ◽  
Vol 38 (6-7) ◽  
pp. 616-624 ◽  
Author(s):  
Hiroyuki Osaki ◽  
Tetsuo Endo

Author(s):  
M. Yamada ◽  
T. Yoshihara ◽  
H. Arima ◽  
Y. Nimura ◽  
T. Kobayashi ◽  
...  

This paper presents the development of a new, fully digital image processing system for a field emission SEM (FE-SEM) which can eliminate fluctuations in the emission current. The system can display, in real time, images with 1280 × 1024 pixels on a viewing CRT, allowing direct observation of high definition SEM images on the FE-SEM.In the past, even though image was acquired and recorded (photographed) with the resolution higher than 1,000 x 1,000 pixels, it was generally observed on the viewing CRT at the resolution of only about 500 x 500 pixels for real-time observation. This created a difference in image quality between the two CRT's. Using a CRT of the same pixel resolution for observation as on the recording CRT, the difference in image quality can be minimised, provided that the characteristics (speed and resolution) of the image processing and scan generator system are improved and optimised for digital processing.


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