The incidence of virulence factors in mesophilicAeromonasspecies isolated from farm animals and their environment

SUMMARYSixty-one isolates ofAeromonasspp. from the faeces of pigs, cows and a variety of associated environmental sources were examined for the characteristics that are reputed to have roles in pathogenicity. Most isolates ofAeromonas hydrophilawere cytotoxic (96·4%) and were capable of producing cell elongation factor (75%) and haemagglutinins (67·9%). In contrast few of theAeromonas caviaeisolates produced these three markers (13·6 %, 27·3% and 36·4% respectively). In general,Aeromonas sobriaoccupied an intermediate position (36·4%, 27·3% and 54·5%), but they did produce the highest mean invasion index for HEp-2 cells. Statistical analysis revealed significant associations between the carriage of these factors and it was clear that many isolates of aeromonads from water and animals possessed the full battery of putative virulence factors.

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Isolation and identification of motile Aeromonas spp.from different sources

Journal of Biotechnology Research Center ◽

10.24126/jobrc.2011.5.3.185 ◽

2011 ◽

Vol 5 (3) ◽

pp. 56-65

Author(s):

Samira Y. Yousif ◽

Rasha Abid Ali Al-Khalidi

Keyword(s):

Aeromonas Hydrophila ◽

Antibiotic Susceptibility ◽

Morphological Characterization ◽

Isolation And Identification ◽

Aeromonas Sobria ◽

Aeromonas Caviae ◽

Susceptibility Tests ◽

Different Sources

Total of 507 samples (clinical, environmental, food) were collected from different hospitals in Baghdad, water, soil, and different food stuffs. Biochemical and morphological characterization tests showed that seventeen isolates were identified as Aeromonas spp.These were farther characterized as Aeromonas hydrophila 10 isolates, Aeromonas sobria 2 isolates, Aeromonas eucrenophila 3 isolates, one isolate belongs to Aeromonas caviae and another one belongs to Aeromonas schubertii. Antibiotic susceptibility tests of all the isolates towards fifteen antibiotics agents were carried out and results showed that all isolates 100% were resistant to penicillin, ampicillin, ampiclox, 99% were resistant to lincomycin, 76.7% to cephalothin, 52.9% to cefotaxime. All isolates except one isolate of Aeromonas eucrenophila were sensitive to meropenem.

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Identification of hydroxy fatty acids in Aeromonas hydrophila, Aeromonas sobria, and Aeromonas caviae.

Journal of Clinical Microbiology ◽

10.1128/jcm.22.6.1061-1062.1985 ◽

1985 ◽

Vol 22 (6) ◽

pp. 1061-1062 ◽

Cited By ~ 5

Author(s):

F P Canonica ◽

M A Pisano

Keyword(s):

Fatty Acids ◽

Aeromonas Hydrophila ◽

Hydroxy Fatty Acids ◽

Aeromonas Sobria ◽

Aeromonas Caviae

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Low Incidence of Aeromonas sp. in Livestock Feces

Journal of Food Protection ◽

10.4315/0362-028x-50.1.66 ◽

1987 ◽

Vol 50 (1) ◽

pp. 66-69 ◽

Cited By ~ 19

Author(s):

NORMAN J. STERN ◽

E. S. DRAZEK ◽

S. W. JOSEPH

Keyword(s):

Aeromonas Hydrophila ◽

Recovery Rate ◽

Agricultural Research ◽

Agar Medium ◽

Brain Heart Infusion ◽

Aeromonas Sobria ◽

Aeromonas Caviae ◽

Retail Meat ◽

Agar Media ◽

Low Incidence

Pig, beef, sheep and turkey fecal specimens were assayed for recovery of inoculated Aeromonas sp. by directly plating the samples on five different agar media. Of these, starch-ampicillin was optimal with respect to selectivity and ability to differentiate from other resident microflora. Generally, the numbers of inoculated Aeromonas sp. recovered on starch-ampicillin agar were similar to those recovered on brain heart infusion and blood ampicillin agar media, and were 101 to 103 greater than the recovery rate on either MacConkey-ampicillin or cefsulodinirgasan-novobiocin agars. The sensitivity for the direct recovery of Aeromonas sp. from inoculated beef feces with naturally contaminating microflora, using streaked starch-ampicillin agar medium, was between 102 and 103 cells per gram. Using starch-ampicillin agar, the incidence of Aeromonas detected from feces of beef, pig, sheep and turkey held at the Beltsville Agricultural Research Center was one of 32, none of 22, none of 24 and three of 21, respectively. Based upon current taxonomic criteria, the isolate from the beef feces had characteristics consistent with both Aeromonas sobria and Aeromonas caviae, whereas three isolates from turkey feces were identified as A. caviae or Aeromonas hydrophila. The organism was isolated from five of five packages of ground beef from retail sources. The discrepancy in the consistent presence of the organism in retail meat suggests that many of the food isolates are probably not of fecal origin.

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