scholarly journals Isolation and identification of motile Aeromonas spp.from different sources

2011 ◽  
Vol 5 (3) ◽  
pp. 56-65
Author(s):  
Samira Y. Yousif ◽  
Rasha Abid Ali Al-Khalidi
Keyword(s):  
Aeromonas Hydrophila ◽  
Antibiotic Susceptibility ◽  
Morphological Characterization ◽  
Isolation And Identification ◽  
Aeromonas Sobria ◽  
Aeromonas Caviae ◽  
Susceptibility Tests ◽  
Different Sources

Total of 507 samples (clinical, environmental, food) were collected from different hospitals in Baghdad, water, soil, and different food stuffs. Biochemical and morphological characterization tests showed that seventeen isolates were identified as Aeromonas spp.These were farther characterized as Aeromonas hydrophila 10 isolates, Aeromonas sobria 2 isolates, Aeromonas eucrenophila 3 isolates, one isolate belongs to Aeromonas caviae and another one belongs to Aeromonas schubertii. Antibiotic susceptibility tests of all the isolates towards fifteen antibiotics agents were carried out and results showed that all isolates 100% were resistant to penicillin, ampicillin, ampiclox, 99% were resistant to lincomycin, 76.7% to cephalothin, 52.9% to cefotaxime. All isolates except one isolate of Aeromonas eucrenophila were sensitive to meropenem.

Low Incidence of Aeromonas sp. in Livestock Feces

1987 ◽  
Vol 50 (1) ◽  
pp. 66-69 ◽  
Author(s):  
NORMAN J. STERN ◽  
E. S. DRAZEK ◽  
S. W. JOSEPH
Keyword(s):  
Aeromonas Hydrophila ◽  
Recovery Rate ◽  
Agricultural Research ◽  
Agar Medium ◽  
Brain Heart Infusion ◽  
Aeromonas Sobria ◽  
Aeromonas Caviae ◽  
Retail Meat ◽  
Agar Media ◽  
Low Incidence

Pig, beef, sheep and turkey fecal specimens were assayed for recovery of inoculated Aeromonas sp. by directly plating the samples on five different agar media. Of these, starch-ampicillin was optimal with respect to selectivity and ability to differentiate from other resident microflora. Generally, the numbers of inoculated Aeromonas sp. recovered on starch-ampicillin agar were similar to those recovered on brain heart infusion and blood ampicillin agar media, and were 101 to 103 greater than the recovery rate on either MacConkey-ampicillin or cefsulodinirgasan-novobiocin agars. The sensitivity for the direct recovery of Aeromonas sp. from inoculated beef feces with naturally contaminating microflora, using streaked starch-ampicillin agar medium, was between 102 and 103 cells per gram. Using starch-ampicillin agar, the incidence of Aeromonas detected from feces of beef, pig, sheep and turkey held at the Beltsville Agricultural Research Center was one of 32, none of 22, none of 24 and three of 21, respectively. Based upon current taxonomic criteria, the isolate from the beef feces had characteristics consistent with both Aeromonas sobria and Aeromonas caviae, whereas three isolates from turkey feces were identified as A. caviae or Aeromonas hydrophila. The organism was isolated from five of five packages of ground beef from retail sources. The discrepancy in the consistent presence of the organism in retail meat suggests that many of the food isolates are probably not of fecal origin.

Isolation and Identification of Aeromonas spp. from Ground Meats in Eastern Canada

1997 ◽  
Vol 60 (2) ◽  
pp. 125-130 ◽  
Author(s):  
UMADATT SINGH
Keyword(s):  
Ground Beef ◽  
Species Level ◽  
Ground Meat ◽  
Eastern Canada ◽  
Isolation And Identification ◽  
Isolation Medium ◽  
Aeromonas Sobria ◽  
Aeromonas Caviae ◽  
Meat Samples ◽  
Better Than

Two commercially available media, Ryan's aeromonas medium (RAM) and GSP agar pseudomonas aeromonas selective agar base (GSP) and one laboratory prepared medium, starch ampicillin agar (SAA), were compared for their ability to recover Aeromonas spp. from pure culture, raw ground beef, and spiked autoclaved ground beef samples. In all instances SAA medium proved to be superior for recovery of Aeromonas spp. Selectivity with SAA and GSP was better than with RAM with 100% of typical colonies confirming as Aeromonas spp. The incidence of motile Aeromonas spp. in ground meat samples in Eastern Canada was determined during a 1-year period using SAA as the isolation medium. Aeromonas spp. was found in 4 of 4 ground pork, chicken, turkey, and sausage samples and in 15 of 19 ground beef samples. Two hundred and ten presumptive Aeromonas isolates were characterized biochemically to the species level. Ninety-seven percent of the isolates from pork and 87% from ground beef were identified as Aeromonas hydrophila. Of the isolates from chicken and turkey, 40 and 56% respectively were found to be this latter species. The numbers of Aeromonas sobria and Aeromonas caviae isolated from these products were 30 and 20% for chicken and 8 and 16% for turkey respectively.

Survival of Aeromonas hydrophila, Aeromonas caviae and Aeromonas sobria in soil

1996 ◽  
Vol 81 (4) ◽  
pp. 439-444 ◽  
Author(s):  
G. Brandi ◽  
M. Sisti ◽  
G. F. Schiavano ◽  
L. Salvaggio ◽  
A. Albano
Keyword(s):  
Aeromonas Hydrophila ◽  
Aeromonas Sobria ◽  
Aeromonas Caviae

scholarly journals Antibiotic susceptibility and pathogenicity of Aeromonas hydrophila isolated from red hybrid tilapia (Oreochromis niloticus×Oreochromis mossambicus) in Malaysia

2020 ◽  
Vol 13 (10) ◽  
pp. 2166-2171 ◽  
Author(s):  
Nurfarahin Ahmad Pauzi ◽  
Nurliyana Mohamad ◽  
Mohamad Azzam-Sayuti ◽  
Ina Salwany Md. Yasin ◽  
Mohd Zamri Saad ◽  
...  
Keyword(s):  
Aeromonas Hydrophila ◽  
Antibiotic Susceptibility ◽  
Bacterial Infections ◽  
Infected Fish ◽  
The Body ◽  
Limited Information ◽  
Tubular Necrosis ◽  
Wide Range ◽  
Mar Index ◽  
Susceptibility Tests

Background and Aim: Aeromonas hydrophila is a major cause of bacterial infections affecting a wide range of warm water fishes worldwide. In Malaysia, A. hydrophila isolations from diseased fishes were previously reported; however, with limited information. The present study investigates the antibiotic susceptibility and pathogenicity of A. hydrophila isolated from farmed red hybrid tilapia (Oreochromis spp.) in Malaysia. Materials and Methods: A. hydrophila was biochemically identified and subjected to antibiotic susceptibility tests. The isolate was then intraperitoneally injected into red hybrid tilapia, and the mortality, clinicopathological changes, and LD50 were determined up to 240 h post-infection (hpi). Results: The isolate demonstrated multiple antibiotic resistances (MAR) toward amikacin, ampicillin, cefotaxime, amoxicillin, trimethoprim-sulfamethoxazole, erythromycin, and streptomycin, with a MAR index of 0.5. The experimental infection of A. hydrophila at 105 CFU/mL in the red hybrid tilapia resulted in 100% mortality at 240 hpi. The LD50 was determined at 1.1×104 CFU/mL. Infected fish demonstrated occasional erratic swimming patterns, localized hemorrhages and depigmentation on the body and operculum areas, fin erosion, enlargement of the gall bladder, and hemorrhage in internal organs. Microscopic observation of infected fish revealed brain congestion, tubular necrosis, and glomerular shrinkage in the kidneys, necrosis of hepatocytes, and congestion of blood vessels in the liver. Conclusion: The high virulence of A. hydrophila to the red hybrid tilapia emphasizes the importance of active, on-going monitoring of its prevalence in Malaysian tilapia farming.

Survival of Aeromonas hydrophila, Aeromonas caviae and Aeromonas sobria in soil

1996 ◽  
Vol 81 (4) ◽  
pp. 439-444
Author(s):  
G. Brandi ◽  
M. Sisti ◽  
G. F. Schiavano ◽  
L. Salvaggio ◽  
A. Albano
Keyword(s):  
Aeromonas Hydrophila ◽  
Aeromonas Sobria ◽  
Aeromonas Caviae
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