Isolation and characterization of an expressed napin gene fromBrassica rapa

1991 ◽  
Vol 1 (4) ◽  
pp. 209-219 ◽  
Author(s):  
Jean C. Kridl ◽  
David W. McCarter ◽  
Ronald E. Rose ◽  
Donna E. Scherer ◽  
Deborah S. Knutzon ◽  
...  
Keyword(s):  
Brassica Rapa ◽  
Storage Protein ◽  
Protein Gene ◽  
Coding Region ◽  
Coding Regions ◽  
Endogenous Expression ◽  
Isolation And Characterization ◽  
Glucuronidase Reporter ◽  
Transgenic Rapeseed

AbstractAn expressed napin storage protein gene fromBrassica rapa, BcNA1, has been cloned and sequenced. The gene is a member of a family of four to seven napin genes inB. rapaand is highly expressed in developing seeds. An expression cassette containing the DNA flanking the napin coding region of BcNA1 has been engineered and demonstrated to function appropriately, as compared with the gene's endogenous expression, in transgenic rapeseed using the β-glucuronidase reporter gene. TheB. rapaBcNA1 gene and aB. napusnapin gene, gNa, share extremely high nucleotide homology not only throughout their coding regions, but over a DNA locus comprising 4.3 kb. We suggest the gNa gene was contributed by the originalB. rapaprogenitor of the amphidiploidB. napus.

scholarly journals Identification of Six Putative Novel Human Papillomaviruses (HPV) and Characterization of Candidate HPV Type 87

2001 ◽  
Vol 75 (23) ◽  
pp. 11913-11919 ◽  
Author(s):  
Stefano Menzo ◽  
Alessia Monachetti ◽  
Caterina Trozzi ◽  
Andrea Ciavattini ◽  
Guido Carloni ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Biological Data ◽  
Human Papillomaviruses ◽  
The Novel ◽  
Tissue Cultures ◽  
Coding Region ◽  
Coding Regions ◽  
Immunodeficiency Virus ◽  
E6 And E7

ABSTRACT Six putative novel human papillomavirus (HPV) types were detected by using general primers for a conserved L1 HPV region in patients examined in gynecologic centers. One of the isolates, detected in samples from 4 patients with koilocytic atypia at cervical cytology (3 of whom were also infected with human immunodeficiency virus type 1), was completely sequenced, identified as a new HPV genotype, and designated candidate HPV87 (candHPV87) by the Reference Center for Human Papillomavirus. candHPV87 shows the classic HPV genome organization and the absence of a functional E5 coding region. Phylogenetic analysis documented that thecandHPV87 genome clusters within the A3 group of HPVs, together with HPV61, HPV72, HPV83, HPV84 and candHPV86, which have been completely sequenced, and a number of other putative novel genotypes (two of which are described in this work), which have been partially characterized. To address the growth-enhancing potential of candHPV87, the E6 and E7 putative coding regions were cloned and expressed in tissue cultures. The data indicate that both proteins stimulate cell division in tissue cultures more than those of low-risk HPVs, though not as much as those of HPV16. Taken together, the clinical, molecular, and biological data suggest that the novel papillomavirus characterized in the present study is a low- to intermediate-risk HPV.

Isolation and characterization of the human gene encodingI to: further diversity by alternative mRNA splicing

1998 ◽  
Vol 275 (6) ◽  
pp. H1963-H1970 ◽  
Author(s):  
Wei Kong ◽  
Sunny Po ◽  
Toshio Yamagishi ◽  
M. Dominique Ashen ◽  
Gail Stetten ◽  
...  
Keyword(s):  
Splice Variants ◽  
Phosphorylation Site ◽  
Coding Region ◽  
Α Subunit ◽  
Isolation And Characterization ◽  
Kinase C ◽  
Alternative Mrna Splicing ◽  
Carboxyl Terminal ◽  
Membrane Spanning

The transient outward K+ current ( I to) in the heart is responsible for the initial phase of repolarization and for setting the plateau voltage of the ventricular action potential. Recently, Kv4.3 has emerged as the leading candidate α-subunit gene that underlies I to in larger mammals such as dogs and humans. We have cloned the human Kv4.3 homolog and describe a carboxyl-terminal splice variant that inserts 19 amino acids with a consensus protein kinase C (PKC) phosphorylation site into the protein after the last membrane-spanning segment. The coding region of Kv4.3 is comprised of at least five exons and is located on chromosome 1p13.3. In the basal state the basic biophysical properties of both of the splice variants are identical.

Isolation and characterization of an auxin-binding protein gene from radish, and its expression in insect cells

1997 ◽  
Vol 101 (3) ◽  
pp. 606-611 ◽  
Author(s):  
Toyoaki Anai ◽  
Ryota Takai ◽  
Momo Miyata ◽  
Hidenobu Uchida ◽  
Seiji Kosemura ◽  
...  
Keyword(s):  
Insect Cells ◽  
Protein Gene ◽  
Binding Protein ◽  
Isolation And Characterization ◽  
Auxin Binding ◽  
Binding Protein Gene ◽  
Auxin Binding Protein

Isolation and characterization of an auxin-binding protein gene from radish, and its expression in insect cells

1997 ◽  
Vol 101 (3) ◽  
pp. 606-611 ◽  
Author(s):  
Toyoaki Anai ◽  
Ryota Takai ◽  
Momo Miyata ◽  
Hidenobu Uchida ◽  
Seiji Kosemura ◽  
...  
Keyword(s):  
Insect Cells ◽  
Protein Gene ◽  
Binding Protein ◽  
Isolation And Characterization ◽  
Auxin Binding ◽  
Binding Protein Gene ◽  
Auxin Binding Protein

Isolation and characterization of channel catfish natural resistance associated macrophage protein gene

2002 ◽  
Vol 26 (6) ◽  
pp. 517-531 ◽  
Author(s):  
Hai Chen ◽  
Geoffrey C. Waldbieser ◽  
Charles D. Rice ◽  
Banu Elibol ◽  
William R. Wolters ◽  
...  
Keyword(s):  
Channel Catfish ◽  
Protein Gene ◽  
Natural Resistance ◽  
Isolation And Characterization ◽  
Macrophage Protein

scholarly journals Molecular isolation and characterization of a novel occlusion body protein gene from Penaeus monodon nucleopolyhedrovirus

Virology ◽  
2008 ◽  
Vol 381 (2) ◽  
pp. 261-267 ◽  
Author(s):  
Parin Chaivisuthangkura ◽  
Chutithorn Tawilert ◽  
Thanawan Tejangkura ◽  
Sombat Rukpratanporn ◽  
Siwaporn Longyant ◽  
...  
Keyword(s):  
Protein Gene ◽  
Penaeus Monodon ◽  
Occlusion Body ◽  
Body Protein ◽  
Isolation And Characterization
Sign in / Sign up

Export Citation Format

Share Document