Distribution of Filipin in Skeletal Muscle Membranes
Filipin, a polyene antibiotic, forms complexes (FC) with cholesterol-like substances (1,2). These complexes were visible by EM as "caveolae" either in clusters or singly (cf.Figs.1,3). They covered the entire plasmalemma, including the transverse tubules (TT) and were present in some mitochondria, at the edges of lipid droplets, and in the sarcoplasmic reticulum (SR).In the SR they were found mainly in the intermediate cisternae (IC),chiefIy in the form of clusters (Fig.3) which were often in register with N lines. FCs were significantly less common in the junctional SR and free SR, where, as in the plasmalemma, they were more likely to be in the single form (Fig.2A). This distribution varied somewhat from fiber to fiber, but not within a sin-gle fiber, nor within 1 or 4 hrs exposure to Filipin.