2G Alters the Utricular Macula of Chick Embryos

1998 ◽  
Vol 4 (S2) ◽  
pp. 1168-1169
Author(s):  
Hirotaka Hara ◽  
César D. Fermin
Keyword(s):  
Constant Velocity ◽  
Imaging System ◽  
Osmic Acid ◽  
Chick Embryos ◽  
Utricular Macula ◽  
Original Apparatus ◽  
Semithin Sections ◽  
System 2 ◽  
Center Axis ◽  
Ultrathin Sections

Chick embryos (Gallus domesticus) exposed continuously from day one until 18 days of incubation to a 2G acceleration (hyperG chicks) force hatched with altered macular epithelia. Fertile eggs were placed in an incubator and exposed to 2G on a centrifuge by the original apparatus until 18 days of incubation. Effective 2G loads were obtained by rotating the apparatus at a constant velocity of 327.5 degree/sec with the incubator mounted at 55 cm from the center axis [1]. The vestibules of the newly hatch chicks were dissected and fixed in 2.5% glutaraldehyde. The utricles were rinsed in phosphate buffer, postfixed with 2% osmic acid and dehydrated in graded ethanol and embedded in plastic. Semithin sections were examined under Light microscopy with computerized video imaging system [2] and ultrathin sections were examined under TEM.

Hemoglobin crystals of the red blood cells in the human renal cortex: electron microscopic observations of the renal lithiasis

1978 ◽  
Vol 36 (2) ◽  
pp. 480-481
Author(s):  
Kosuke Ueda ◽  
Hiroto Washida ◽  
Nakazo Watari
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Renal Cortex ◽  
Uranyl Acetate ◽  
Osmic Acid ◽  
Renal Lithiasis ◽  
Electron Microscopic ◽  
Hemoglobin C ◽  
First Case ◽  
Ultrathin Sections

IntroductionHemoglobin crystals in the red blood cells were electronmicroscopically reported by Fawcett in the cat myocardium. In the human, Lessin revealed crystal-containing cells in the periphral blood of hemoglobin C disease patients. We found the hemoglobin crystals and its agglutination in the erythrocytes in the renal cortex of the human renal lithiasis, and these patients had no hematological abnormalities or other diseases out of the renal lithiasis. Hemoglobin crystals in the human erythrocytes were confirmed to be the first case in the kidney.Material and MethodsTen cases of the human renal biopsies were performed on the operations of the seven pyelolithotomies and three ureterolithotomies. The each specimens were primarily fixed in cacodylate buffered 3. 0% glutaraldehyde and post fixed in osmic acid, dehydrated in graded concentrations of ethanol, and then embedded in Epon 812. Ultrathin sections, cut on LKB microtome, were doubly stained with uranyl acetate and lead citrate.

Immunocytochemical localization of desmin and vimentin in chick embryonic myocardial cells

1990 ◽  
Vol 48 (3) ◽  
pp. 448-449
Author(s):  
Yukiko Sugi
Keyword(s):  
Sodium Methoxide ◽  
Intermediate Filament Protein ◽  
Chick Embryos ◽  
Immunocytochemical Localization ◽  
Myocardial Cells ◽  
Immunofluorescence Study ◽  
Immunofluorescent Localization ◽  
Rabbit Igg ◽  
Semithin Sections ◽  
Filament Protein

In cultured skeletal muscle cells of chick, one intermediate filament protein, vimentin, is primarily formed and then synthesis of desmin follows. Coexistence of vimentin and desmin has been immunocytochemically confirmed in chick embryonic skeletal musclecells. Immunofluorescent localization of vimentin and desmin has been described in developing myocardial cells of hamster. However, initial localization of desmin and vimentin in early embryonic heart has not been reported in detail. By quick-freeze deep-etch method a loose network of intermediate filaments was revealed to exist surrounding myofibrils. In this report, immunocytochemical localization of desmin and vimentin is visualized in early stages of chick embryonic my ocardium.Chick embryos, Hamburger-Hamilton (H-H) stage 8 to hatch, and 1 day old postnatal chicks were used in this study. For immunofluorescence study, each embryo was fixed with 4% paraformaldehyde and embedded in Epon 812. De-epoxinized with sodium methoxide, semithin sections were stained with primary antibodies (rabbit anti-desmin antibody and anti-vimentin antibody)and secondary antibody (RITC conjugated goat-anti rabbit IgG).

Microwave Polymerization of Embedding Resins for Biological/Biomedical Elecron Microscopy

1990 ◽  
Vol 189 ◽  
Author(s):  
B.L. Giammara ◽  
D.J. Birch ◽  
D.O. Harper
Keyword(s):  
Epoxy Resins ◽  
Polyester Resin ◽  
Unsaturated Polyester ◽  
Polymerization Time ◽  
Diagnostic Pathology ◽  
Rapid Methods ◽  
Convection Oven ◽  
Microwave Polymerization ◽  
Semithin Sections ◽  
Ultrathin Sections

ABSTRACTThe use of microwave energies for polymerization of epoxy and other commercially available resins, used routinely in electron microscopy methodologies, shows promise for diagnostic pathology where rapid methods can be crucial. Many desirable embedment properties, such as specimen infiltration, color, hardness, rough trimming, and ability to stain, are necesssary. The properties necessary for cutting 1 micron semithin sections and 700 Angstrom ultrathin sections (that can withstand pentration by a 100 kV electron beam for image formation) must be maintained.In this study, six epoxy resins and one unsaturated polyester resin were used, the latter in a variety of recipes. Each formulation was subjected to microwave power levels from 400 to 700 W for 1 to 15 minutes. Selected specimen embedments tested well and significantly reduced traditional convection oven polymerization time from two days to a few minutes.

Ultrastructure of the decidual cells of the basal plate of the human placenta at term

1978 ◽  
Vol 36 (2) ◽  
pp. 564-565
Author(s):  
C. OliVar ◽  
S. Castejon
Keyword(s):  
Human Placenta ◽  
Phosphate Buffer ◽  
Uranyl Acetate ◽  
Basal Plate ◽  
Osmic Acid ◽  
Intercellular Substance ◽  
Decidual Cells ◽  
Ultrathin Sections ◽  
Spontaneous Delivery

To understand the role of the decidual cells of the basal plate of the human placenta at term in the passage of the antigens and antibodies, in the production of hormones, in the nutrition of the embryo and its possible function in the formation of fibrilar proteins, a systematic study of their ultrastructure has been necessary.Immediately following a spontaneous delivery, samples of the decidua were taken and fixed by immersion in 2% g1utara1dehyde in phosphate buffer 0. 2 M (PH 7. 4). The specimens were washed in a similar buffer for 1 hour and postfixed in 2% osmic acid in phosphate buffer 0. 2 for 3 hours at 4°C. After dehydrating the samples in a graded series of ethanols and propylene oxide they were embedded in Araldite. Ultrathin sections were then stained with uranyl acetate and lead tricitrate and examined under the electron microscope. The submicroscopic observations show that: The plasmamembrane of these cells demonstrates numerous irregular, globulous or polyhedral extensions (Fig. 1a), which intermix with the intercellular substance or connect with adjacent cells by means of desmosomes (Fig. 1b). The hyaloplasmic matrix (Fig. 2) is of low electron density, homogenous, with numerous free ribosomes and cytoplasmic microfilaments (Fig. 4) which are arranged in bundles dispersed throughout the cytoplasm in different directions.

scholarly journals Immunocytochemical studies of cardiac myofibrillogenesis in early chick embryos. II. Generation of alpha-actinin dots within titin spots at the time of the first myofibril formation.

1987 ◽  
Vol 105 (6) ◽  
pp. 2795-2801 ◽  
Author(s):  
K T Tokuyasu ◽  
P A Maher
Keyword(s):  
The Other ◽  
Chick Embryos ◽  
Initial Length ◽  
Electron Microscopic ◽  
Somite Stage ◽  
Dense Bodies ◽  
Whole Mount ◽  
Ultrathin Sections ◽  
Per Se ◽  
Immunocytochemical Studies

In whole mount preparations of the 9 somite stage chick embryonic hearts that were immunofluorescently double labeled for titin and alpha-actinin, presumptive myofibrils were recognized as rows of several periodically aligned titin spots. Within these titin spots, smaller alpha-actinin dots were observed. These periodical arrangements of titin spots and alpha-actinin dots were not found in the 7 somite stage hearts. In wide myofibrils in the 10 somite stage hearts, the alpha-actinin dots and titin spots simultaneously became 'lines.' To study the ultrastructural features of the titin-positive regions in the 6-9 somite stage hearts, the thoracic portions of the embryos were immunofluorescently labeled for titin and embedded in resin. Ultrathin sections were mounted on electron microscopic grids and examined in immunofluorescence optics. The titin-positive regions thus identified were then examined in the electron microscope. No readily discernable specific ultrastructural features were found in titin-positive regions of the 6 somite stage cardiac primodia. Examination of the sections of the 9 somite stage hearts, on the other hand, revealed the occasional presence of small dense bodies, Z bodies, in the titin-positive regions. These observations strongly suggest that these Z bodies are the ultrastructural counterparts of the alpha-actinin dots seen by immunofluorescence optics and that they are formed nearly at the time of the formation of the first myofibrils. In some of the nascent myofibrils the Z bodies were found to be considerably narrower than the myofibrils, implying that the Z bodies are required not for the assembly of myofibrils per se but for their stabilization. Immunofluorescent labeling for titin and alpha-actinin revealed that the length of the shortest sarcomeres in the first myofibrils is approximately 1.5 micron, approximately the width of the A bands of mature myofibrils. The possibility that the A bands might define the initial length of nascent sarcomeres was indicated.

scholarly journals 445. Clinical Applications of Diffusion Imaging System (2)

1992 ◽  
Vol 48 (8) ◽  
pp. 1528
Author(s):  
Yoshimitsu Matsumura ◽  
Kiyoshi Oguro ◽  
Shusaku Harada ◽  
Suguru Kotaki
Keyword(s):  
Imaging System ◽  
Diffusion Imaging ◽  
Clinical Applications ◽  
System 2

Intranuclear inclusions in macrophages of lizards Lacerta agilis, experimentally infected by acanthocephalan Corynosoma strumosum

2018 ◽  
Vol 12 (1) ◽  
pp. 52-58
Author(s):  
V. P. Nikishin ◽  
E. M. Skorobrechova
Keyword(s):  
Methylene Blue ◽  
Microscopic Analysis ◽  
Spherical Shape ◽  
Paratenic Host ◽  
Electron Microscopic ◽  
Electronic Density ◽  
Parasitic Worm ◽  
Semithin Sections ◽  
Ultrathin Sections ◽  
Lacerta Agilis

The purpose of the research: to study the cell response of non-natural paratenic host and encapsulation process of acanthocephalan Corynosoma strumosum in experiment for further comparison with encapsulation mechanism of this acanthocephalan in natural paratenic host. Materials and methods. Experiments were carried out on 24 lizards Lacerta agilis and one L. viridis. 17 encapsulated acanthocephalans were received from 13 of them. Acanthocephalans with capsules were prepared for electron microscopic analysis according to standard methods and examined in light (semithin sections) and under electron (in ultrathin sections) microscopes. Semithin sections were stained with methylene blue or a mixture of methylene blue and crystal violet. Ultrathin sections were stained with lead citrate. All capsules received in the experiment were investigated with the use of the light microscope; 1,5 and 10 day capsules were examined under electron microscope. Results and discussion. All acanthocephalans studied in this paper including those discovered one and half day after the start of experiment were enclosed in the thick cellular capsule with prevailing mononuclear and multinuclear macrophages. Single electron-dense inclusions of regular rounded shape surrounded by hallo of moderately dense material were found in approximately half of both types of nuclei. Nature of inclusions remained unknown. In the interpretation of results, it is necessary to take into account: 1) the presence of these inclusions in macrophage nuclei only; 2) their strictly ordinary positioning in the nucleus; 3) strictly spherical shape; 4) very high electronic density of their material, that exceeds the density of the nucleolus and chromatin; 5) presence of halo; 6) absence of visible pathological signs in nuclei and cell’s cytoplasm where these inclusions had been found. Their appearance is supposed to be connected with the overactivity of lizard macrophages caused by invasion of a parasitic worm.

Electron microscopic study on the phagocytic lining cells in the cavernous body of the lamprey gill

1978 ◽  
Vol 36 (2) ◽  
pp. 448-449
Author(s):  
Kazuhito Yamaguchi ◽  
Kazuhiko Awaya
Keyword(s):  
Electron Microscope ◽  
Uranyl Acetate ◽  
Osmic Acid ◽  
Lead Citrate ◽  
Electron Microscopic ◽  
Embedded Blocks ◽  
Ultrathin Sections ◽  
Branchial Artery ◽  
Scanning Electron ◽  
Cavernous Body

The lining cells in the cavernous body, which is a branch of the afferent branchial artery, of lamprey gills were studied with transmission (TEM) and scanning electron microscope (SEM).Adult and larval lampreys, Lampertra planeri, were used in this study. Lamprey gills removed after perfusion were fixed in 2 % glutaraldehyde/formaldehyde (pH 7. 4) for 1 hour and postfixed in 1 % osmic acid. For TEM, the gills were dehydrated in ethanol and embedded in epon 812. Ultrathin sections were doubly stained with uranyl acetate and lead citrate. For SEM, most of the gills were dehydrated in ethanol and isoamylacetate and cracked in liquid nitrogen. Some of the gills dehydrated were embedded in stylene monomer. Stylene embedded blocks were cracked with a hummer and dissolved in propylene oxide. All the specimens were dried in a critical point dryer and coated 100 Å thickness with gold-paladium.The cavernous body is composed of trabeculae, between which are blood spaces, the lacunae.

Some Ultrastructural Aspects of Chronic Gastritis

1990 ◽  
Vol 48 (3) ◽  
pp. 198-199
Author(s):  
Hieronim Bartel ◽  
Stanislaw Orkisz ◽  
Jan Chojnacki
Keyword(s):  
Chronic Gastritis ◽  
Gastric Epithelium ◽  
Ultrastructural Changes ◽  
Campylobacter Pylori ◽  
Surface Epithelium ◽  
Biopsy Material ◽  
Campylobacter Pyloridis ◽  
Semithin Sections ◽  
Ultrathin Sections ◽  
Epithelium Cells

Despite numerous observations and publications on the aethiology and pathogenesis of chronic gastritis many questions remain unanswered (1,2,3,4,6). Views on the aethiolcgy of chronic gastritis changed when in 1982 Campylobacter pyloridis (later named Campylobacter pylori CP) was first isolated in Western Australia from the stomach of patients with gastritis lesions and peptic ulceration (5).The aim of this study, carried out with cooperation with gastroenterologist, has been to investigate the ultrastructural changes in gastric surface epithelium and lamina propria in selected patients endoscopicaUy diagnosed gastritis.Observations have been carried out on 20 patients (10 men and 10 women). Biopsy material was obtained during endoscopy. In cases when the presence of bacteria has been confirmed further E M investigations have been introduced. Semithin sections for LM observations and ultrathin sections for EM were prepared according to routine EM technique.CP was found both on the airface of gastric epithelium cells, and what has not been reported so far, inside the lumen of the fundic glands (Fig. 1).

Sign in / Sign up

Export Citation Format

Share Document