Negative Stain Transmission Electron Microscopy of Viruses and Virus-like Particles

Lagena radicicola Vanterpool & Ledingham is an obligate parasite inside root hairs and epidermal cells. It was cultured in a unifungal state on wheat in pots. The life cycle was examined by both light and transmission electron microscopy. The thallus developed inside a single host cell and formed either a single sporangium or one to four resting spores. Zoospore cleavage was completed in vesicles outside the root. The resting spores were similar to oospores in their development and cytology, but there was no evidence of cell fusion and sexuality. Virus-like particles were seen in 3- to 12-month-old cultures, and infected cells became degenerate. Key words: Oomycetes, ultrastructure, virus-like particles, biocontrol.

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Ultramicroscopic observation of recombinant adeno-associated virus type 2 on the surface of formvar-carbon coated copper grids under different relative humidity and incubation time using negative stain transmission electron microscopy

AFRICAN JOURNAL OF BIOTECHNOLOGY ◽

10.5897/ajb2009.000-9466 ◽

2009 ◽

Vol 8 (21) ◽

pp. 5790-5798

Author(s):

Liu Shu Peng ◽

Niu Chun Ying ◽

Li Xue Ling ◽

Chen Heng

Keyword(s):

Electron Microscopy ◽

Transmission Electron Microscopy ◽

Relative Humidity ◽

Virus Type ◽

Incubation Time ◽

Adeno Associated Virus ◽

Negative Stain ◽

Transmission Electron ◽

Carbon Coated

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Interfacing Microfluidics with Negative Stain Transmission Electron Microscopy

Analytical Chemistry ◽

10.1021/acs.analchem.5b03884 ◽

2015 ◽

Vol 88 (1) ◽

pp. 629-634 ◽

Cited By ~ 5

Author(s):

Nikita Mukhitov ◽

John M. Spear ◽

Scott M. Stagg ◽

Michael G. Roper

Keyword(s):

Electron Microscopy ◽

Transmission Electron Microscopy ◽

Negative Stain ◽

Transmission Electron

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Visualization of Meiotic Chromosomes by Negative-Stain Transmission Electron Microscopy: Preparation and Staining Methods Using Common Staining Reagents

Microscopy and Microanalysis ◽

10.1017/s1431927606066852 ◽

2006 ◽

Vol 12 (S02) ◽

pp. 284-285

Author(s):

MJ de la Cruz ◽

S Lockett ◽

K Nagashima

Keyword(s):

Electron Microscopy ◽

Transmission Electron Microscopy ◽

Meiotic Chromosomes ◽

Negative Stain ◽

Transmission Electron ◽

Staining Methods

Extended abstract of a paper presented at Microscopy and Microanalysis 2006 in Chicago, Illinois, USA, July 30 – August 3, 2005

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Characterization of virus-like particles in GARDASIL® by cryo transmission electron microscopy

Human Vaccines & Immunotherapeutics ◽

10.4161/hv.27316 ◽

2013 ◽

Vol 10 (3) ◽

pp. 734-739 ◽

Cited By ~ 34

Author(s):

Qinjian Zhao ◽

Clinton S Potter ◽

Bridget Carragher ◽

Gabriel Lander ◽

Jaime Sworen ◽

...

Keyword(s):

Electron Microscopy ◽

Transmission Electron Microscopy ◽

Virus Like Particles ◽

Transmission Electron

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Techniques for Transmission Electron Microscopy of Fiber-Matrix Interfaces in Aluminum Alloy-Boron Composites by Ion Erosio

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100065079 ◽

1971 ◽

Vol 29 ◽

pp. 204-205

Author(s):

G. G. Shaw

Keyword(s):

Electron Microscopy ◽

Transmission Electron Microscopy ◽

Aluminum Alloy ◽

Electron Beam ◽

Pure Aluminum ◽

Ion Milling ◽

Transmission Electron ◽

Fiber Matrix ◽

Ion Erosion ◽

Row Of Fibers

The morphology and composition of the fiber-matrix interface can best be studied by transmission electron microscopy and electron diffraction. For some composites satisfactory samples can be prepared by electropolishing. For others such as aluminum alloy-boron composites ion erosion is necessary.When one wishes to examine a specimen with the electron beam perpendicular to the fiber, preparation is as follows: A 1/8 in. disk is cut from the sample with a cylindrical tool by spark machining. Thin slices, 5 mils thick, containing one row of fibers, are then, spark-machined from the disk. After spark machining, the slice is carefully polished with diamond paste until the row of fibers is exposed on each side, as shown in Figure 1.In the case where examination is desired with the electron beam parallel to the fiber, preparation is as follows: Experimental composites are usually 50 mils or less in thickness so an auxiliary holder is necessary during ion milling and for easy transfer to the electron microscope. This holder is pure aluminum sheet, 3 mils thick.

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Direct Observation of Heat Exchanger Deposits by Cross Sectioning

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100061835 ◽

1967 ◽

Vol 25 ◽

pp. 364-365

Author(s):

R. W. Anderson ◽

D. L. Senecal

Keyword(s):

Electron Microscopy ◽

Transmission Electron Microscopy ◽

Heat Exchanger ◽

Direct Observation ◽

Cross Sections ◽

Preparation Method ◽

Specimen Preparation ◽

Flowing Water ◽

Transmission Electron ◽

Deposit Layer

A problem was presented to observe the packing densities of deposits of sub-micron corrosion product particles. The deposits were 5-100 mils thick and had formed on the inside surfaces of 3/8 inch diameter Zircaloy-2 heat exchanger tubes. The particles were iron oxides deposited from flowing water and consequently were only weakly bonded. Particular care was required during handling to preserve the original formations of the deposits. The specimen preparation method described below allowed direct observation of cross sections of the deposit layers by transmission electron microscopy.The specimens were short sections of the tubes (about 3 inches long) that were carefully cut from the systems. The insides of the tube sections were first coated with a thin layer of a fluid epoxy resin by dipping. This coating served to impregnate the deposit layer as well as to protect the layer if subsequent handling were required.

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Phase Transformations in Ti-7w/oMo-3w/oMe Alloy

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100052584 ◽

1974 ◽

Vol 32 ◽

pp. 514-515

Author(s):

S. Fujishiro

Keyword(s):

Electron Microscopy ◽

Mechanical Properties ◽

Transmission Electron Microscopy ◽

Tensile Strength ◽

Mechanical Processing ◽

Ray Method ◽

X Ray ◽

Transmission Electron ◽

Water Quench ◽

Alpha Beta

The mechanical properties of three titanium alloys (Ti-7Mo-3Al, Ti-7Mo- 3Cu and Ti-7Mo-3Ta) were evaluated as function of: 1) Solutionizing in the beta field and aging, 2) Thermal Mechanical Processing in the beta field and aging, 3) Solutionizing in the alpha + beta field and aging. The samples were isothermally aged in the temperature range 300° to 700*C for 4 to 24 hours, followed by a water quench. Transmission electron microscopy and X-ray method were used to identify the phase formed. All three alloys solutionized at 1050°C (beta field) transformed to martensitic alpha (alpha prime) upon being water quenched. Despite this heavily strained alpha prime, which is characterized by microtwins the tensile strength of the as-quenched alloys is relatively low and the elongation is as high as 30%.

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