The life cycle of Lagena radicicola, an oomycetous parasite of wheat roots

Lagena radicicola Vanterpool & Ledingham is an obligate parasite inside root hairs and epidermal cells. It was cultured in a unifungal state on wheat in pots. The life cycle was examined by both light and transmission electron microscopy. The thallus developed inside a single host cell and formed either a single sporangium or one to four resting spores. Zoospore cleavage was completed in vesicles outside the root. The resting spores were similar to oospores in their development and cytology, but there was no evidence of cell fusion and sexuality. Virus-like particles were seen in 3- to 12-month-old cultures, and infected cells became degenerate. Key words: Oomycetes, ultrastructure, virus-like particles, biocontrol.

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Negative Stain Transmission Electron Microscopy of Viruses and Virus-like Particles

Microscopy and Microanalysis ◽

10.1017/s1431927609094446 ◽

2009 ◽

Vol 15 (S2) ◽

pp. 376-377 ◽

Cited By ~ 1

Author(s):

C Humphrey

Keyword(s):

Electron Microscopy ◽

Transmission Electron Microscopy ◽

Virus Like Particles ◽

Negative Stain ◽

Transmission Electron

Extended abstract of a paper presented at Microscopy and Microanalysis 2009 in Richmond, Virginia, USA, July 26 – July 30, 2009

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The vegetative life-cycle of the clover pathogen Cymadothea trifolii as revealed by transmission electron microscopy

Mycological Research ◽

10.1017/s0953756205003035 ◽

2005 ◽

Vol 109 (7) ◽

pp. 764-778 ◽

Cited By ~ 11

Author(s):

Uwe K. Simon ◽

Robert Bauer ◽

Danny Rioux ◽

Marie Simard ◽

Franz Oberwinkler

Keyword(s):

Electron Microscopy ◽

Transmission Electron Microscopy ◽

Life Cycle ◽

Transmission Electron

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Life cycle ofRickettsia slovacain L929 cell line studied by quantitative real-time PCR and transmission electron microscopy

FEMS Microbiology Letters ◽

10.1111/j.1574-6968.2009.01510.x ◽

2009 ◽

Vol 293 (1) ◽

pp. 102-106 ◽

Cited By ~ 6

Author(s):

Vojtech BoldiÅ¡ ◽

Jasna Å trus ◽

Elena KocianovÃ¡ ◽

Magda TuÅ¡ek-Å½nidariÄ ◽

KatarÃna Å tefanidesovÃ¡ ◽

...

Keyword(s):

Electron Microscopy ◽

Transmission Electron Microscopy ◽

Life Cycle ◽

Cell Line ◽

Real Time ◽

Real Time Pcr ◽

L929 Cell ◽

Quantitative Real Time Pcr ◽

Transmission Electron ◽

L929 Cell Line

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Plasmodium falciparum: regional differences in lectin and cationized ferritin binding to the surface of the malaria-infected human erythrocyte

Parasitology ◽

10.1017/s0031182000049799 ◽

1986 ◽

Vol 93 (1) ◽

pp. 17-32 ◽

Cited By ~ 9

Author(s):

I. W. Sherman ◽

Jane R. T. Greenan

Keyword(s):

Electron Microscopy ◽

Transmission Electron Microscopy ◽

Plasmodium Falciparum ◽

Uniform Distribution ◽

Lectin Binding ◽

Red Cells ◽

Cationized Ferritin ◽

Transmission Electron ◽

Erythrocyte Surface ◽

Infected Cells

SUMMARYThe distribution of anionic residues on the surface of erythrocytes infected withPlasmodium falciparumwas studied using cationized ferritin (CF) and transmission electron microscopy. CF staining of uninfected erythrocytes or erythrocytes infected with a knobless variant resulted in a dense and uniform distribution of ferritin particles; however, when red cells infected with a knob-inducing variant were exposed to CF, aggregates of ferritin particles were observed in the region of membrane elevation. Lectin binding to the erythrocyte surface was visualized by transmission electron microscopy using ferritin-conjugated lectins and lectin-fetuin-gold. No differences were observed in the lectin-binding patterns of malaria-infected or uninfected erythrocytes using WGA (wheat-germ agglutinin), RCA (ricin), andLimax flavuslectin. In distinct contrast to the uniform distribution of ferritin particles seen with these lectins was the appearance of clusters of ferritin-ConA over the knobby regions. Localized aggregates of ConA were not seen in knob-free areas or on the surface of red cells infected with a knobless variant. No significant differences were found in the agglutination reactions of normal and infected cells with theCancer antennariuslectin specific forO-acylated sialic acids.

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Effects of miltefosine treatment in fibroblast cell cultures and in mice experimentally infected withNeospora caninumtachyzoites

Parasitology ◽

10.1017/s0031182012000066 ◽

2012 ◽

Vol 139 (7) ◽

pp. 934-944 ◽

Cited By ~ 15

Author(s):

KARIM DEBACHE ◽

ANDREW HEMPHILL

Keyword(s):

Electron Microscopy ◽

Transmission Electron Microscopy ◽

Treatment Group ◽

Neospora Caninum ◽

Fibroblast Cell ◽

Time Span ◽

Human Foreskin Fibroblasts ◽

Transmission Electron ◽

Infected Cells

SUMMARYMiltefosine was investigated for its activity againstNeospora caninumtachyzoitesin vitro,and was shown to inhibit the proliferation ofN. caninumtachyzoites cultured in human foreskin fibroblasts (HFF) with an IC50of 5·2μM. Treatment of infected cells with 25μM miltefosine for a period of 10 h had only a parasitostatic effect, while after 20 h of treatment parasiticidal effects were observed. This was confirmed by transmission electron microscopy ofN. caninum-infected and miltefosine-treated HFF. Administration of miltefosine toN. caninum-infected Balb/c female mice at 40 mg/kg/day for 14 days resulted in 6 out of 10 mice exhibiting weight loss, ruffled coat and apathy between days 7 and 13 post-infection. In the group that received placebo, only 2 out of 8 mice succumbed to infection, but the cerebral burden was significantly higher compared to the miltefosine treatment group. In a second experiment, the time-span of treatment was reduced to 5 days, and mice were maintained without further treatment for 4 weeks. Only 2 out of 9 mice in the miltefosine treatment group exhibited signs of disease, while 8 out of 10 mice succumbed to infection in the placebo group. These results showed that miltefosine hampered the dissemination of parasites into the CNS during experimentalN. caninuminfection in mice.

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Analysis of Mason-Pfizer Monkey Virus Gag Particles by Scanning Transmission Electron Microscopy

Journal of Virology ◽

10.1128/jvi.75.19.9543-9548.2001 ◽

2001 ◽

Vol 75 (19) ◽

pp. 9543-9548 ◽

Cited By ~ 20

Author(s):

Scott D. Parker ◽

Joseph S. Wall ◽

Eric Hunter

Keyword(s):

Electron Microscopy ◽

Transmission Electron Microscopy ◽

Scanning Transmission Electron Microscopy ◽

Particle Mass ◽

Average Mass ◽

Scanning Transmission Electron ◽

Transmission Electron ◽

Scanning Transmission ◽

Infected Cells ◽

The Masses

ABSTRACT Mason-Pfizer monkey virus immature capsids selected from the cytoplasm of baculovirus-infected cells were imaged by scanning transmission electron microscopy. The masses of individual selected Gag particles were measured, and the average mass corresponded to 1,900 to 2,100 Gag polyproteins per particle. A large variation in Gag particle mass was observed within each population measured.

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Effect of One Year of Cryopreservation on the Activity of Lysosomal Hydrolases from EBV-Transformed Lymphocytes

Journal of Biomedicine and Biotechnology ◽

10.1155/2011/132581 ◽

2011 ◽

Vol 2011 ◽

pp. 1-5

Author(s):

A. S. de Mello ◽

F. B. Mendes ◽

K. Michelin-Tireli ◽

M. V. Camelier ◽

J. C. Coelho

Keyword(s):

Electron Microscopy ◽

Transmission Electron Microscopy ◽

Cell Transformation ◽

Morphological Changes ◽

Lysosomal Storage ◽

Lysosomal Hydrolases ◽

Cell Bank ◽

Transmission Electron ◽

Infected Cells ◽

One Year

Background. The Epstein-Barr virus (EBV) was used as an agent of B lymphocyte proliferation for subsequent diagnosis of lysosomal storage disease. Due to the constant handling of long-preserved samples in our cell bank, we decided to observe the behavior and then compare cultured and frozen samples for at least one year's cryopreservation.Methods. Twenty-five samples from healthy individuals were used to assess the possible changes in activity of enzymesβ-galactosidase,β-glucosidase,α-iduronidase,α-galactosidase, andα-glucosidase. Transmission electron microscopy was used to confirm cell transformation of B lymphocytes into EBV-infected cells, generating lymphoblastoid cell lines.Results. Transmission electron microscopy findings confirmed previous reports in the literature that is, significant and evident morphological changes in the nucleus occur after day 12 and the consequent cell transformation into EBV-infected cells. After thawing and subsequent treatment with the five enzymes utilized, we observed no significant changes in samples cryopreserved for more than one year, as compared to samples cultured for 12 days.

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