Characterization of high anthocyanin-producing tetraploid potato cultivars selected for breeding using morphological traits and microsatellite markers

2015 ◽  
Vol 15 (2) ◽  
pp. 147-156 ◽  
Author(s):  
Roberto Tierno ◽  
Jose Ignacio Ruiz de Galarreta
Keyword(s):  
Microsatellite Markers ◽  
Ssr Markers ◽  
Morphological Traits ◽  
Potato Breeding ◽  
Potato Cultivars ◽  
Morphological Descriptors ◽  
Phytochemical Content ◽  
Potato Germplasm ◽  
Potato Breeding Programme

Purple- and red-fleshed potato cultivars constitute a great source of phenolic compounds, which may promote human health. Since the characterization of potato germplasm is a key step during the breeding process, the classification of high anthocyanin-producing tetraploid genotypes may facilitate the incorporation of phenolic-related traits in a potato breeding programme. A set of 18 high anthocyanin-producing underutilized tetraploid cultivars, which have been previously classified in terms of phytochemical content, have been characterized by both microsatellite markers (simple sequence repeat, SSR) and morphological descriptors. A wide genetic variability was found using 11 highly discriminatory SSR markers. The collection also displayed a large amount of variation for most morphological traits. The neighbour-joining trees defined by SSR markers and morphological descriptors revealed genetic and phenotypic relatedness of the potato genotypes. Despite the complexity of tetrasomic inheritance, high anthocyanin-producing tetraploid cultivars should be considered for potato breeding since they are adapted to long-day conditions and do not present undesirable characteristics that are found in native accessions or landraces.

scholarly journals Microsatellite markers for identification of a group of italian olive accessions

2009 ◽  
Vol 66 (5) ◽  
pp. 685-690 ◽  
Author(s):  
Innocenzo Muzzalupo ◽  
Francesca Stefanizzi ◽  
Amelia Salimonti ◽  
Rosanna Falabella ◽  
Enzo Perri
Keyword(s):  
Microsatellite Markers ◽  
Molecular Characterization ◽  
Ssr Markers ◽  
Olea Europaea ◽  
Fruit Trees ◽  
Ssr Primers ◽  
Olive Cultivars ◽  
Olea Europaéa

Cultivar characterization for fruit trees certification requires fast, efficient and reliable techniques. Microsatellite markers (SSR) were used in the molecular characterization of 23 genotypes of Olea europaea subsp europaea. The DNA from the olive cultivars was analyzed using nine pre-selected SSR primers (GAPU59, GAPU71A, GAPU71B, GAPU103A, UDO99-01, UDO99-12, UDO99-28 and UDO99-39) and revealed 29 alleles, which allowed each genotype to be identified. In the dendrogram, the nine primers allowed the 23 olive genotypes to be grouped into subgroups corresponding to the same cultivar denominations. SSR markers proved to be efficient and reliable for the molecular characterization of Italian olive cultivars.

Characterization of Tomentosa cherry (Prunus tomentosa Thunb.) genotypes using SSR markers and morphological traits

2008 ◽  
Vol 118 (1) ◽  
pp. 39-47 ◽  
Author(s):  
Qijing Zhang ◽  
Guijun Yan ◽  
Hongyan Dai ◽  
Xinzhong Zhang ◽  
Chunmin Li ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Morphological Traits

scholarly journals Characterization of Indian Mustard Germplasm on the Basis of Morphological Traits and SSR Markers

2020 ◽  
pp. 300-311
Author(s):  
Narendra Singh Rajpoot ◽  
M. K. Tripathi ◽  
Sushma Tiwari ◽  
R. S. Tomar ◽  
V. S. Kandalkar
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Morphological Traits ◽  
Indian Mustard ◽  
Morphological Characters ◽  
High Genetic Diversity ◽  
Average Value ◽  
Seed Crops ◽  
High Degree

The genus Brassica is one of the most important oil seed crops in India with high degree of genetic diversity. In present study, genetic diversity was studied in forty germplasm lines and eight cultivars of Indian mustard using morphological traits and SSR markers. Morphological characters were taken for days to 50% flowering, days to maturity, plant height (cm), length of main raceme (cm), number of primary branches/plant, number of secondary branches/plant, number of silique per plant, number of seeds per silique, 1000 seed weight (g) and seed yield per plant (g). Total 50 SSR markers were used for characterization of these lines, out of which 7 SSR markers were highly polymorphic between all the germplasms of mustard. An UPGMA phonogram was constructed for all 48 Germplasms and the similarity coefficient ranged from 0.00 to 0.91. Number of alleles ranged from 3 to 4, genetic diversity ranged from 71% to 65% with average value of 67%, heterozygosity raged from 20 to 10% with average of 12% and PIC value for markers ranged from 0.65 to 0.59 with mean PIC value 0.61. All seven SSR primers showed PIC value above 0.5 (50%) indicating high genetic diversity in the studied plant material.

scholarly journals Characterization of Genetic Diversity of Stone Fruit Rootstocks Used in Chile by Means of Microsatellite Markers

2012 ◽  
Vol 137 (5) ◽  
pp. 302-310 ◽  
Author(s):  
María José Arismendi ◽  
Patricio Hinrichsen ◽  
Ruben Almada ◽  
Paula Pimentel ◽  
Manuel Pinto ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Ssr Markers ◽  
Stone Fruit ◽  
Identification System ◽  
Expected Heterozygosity ◽  
Commercially Important ◽  
Taxonomic Groups ◽  
Simple Sequence

Stone fruit (Prunus L.) production in Chile covers ≈43,000 ha and includes a wide variety of soils and climates requiring a large diversity of rootstocks. The most commercially important rootstock cultivars are 26 genotypes from three different taxonomic groups belonging to the subgenera Amygdalus (L.) Benth. Hook. (peach group), Prunus Focke [= Prunophora (Neck.)] Focke (plum group), and Cerasus (Adans.) Focke (cherry group) with eight, seven, and 10 individuals, respectively. To determine their genetic diversity, characterization by microsatellite markers [simple sequence repeat (SSR)] was conducted. Of a total of 20 SSR markers evaluated, 12 generated amplified products that were consistent in the three taxonomic groups. The number of alleles per marker ranged from 18 for PSM-3 to four in CPPCT-002. Clustering analysis, by both traditional hierarchical and model-based approaches, indicate that all genotypes are clustered in their respective taxonomic groups, including the interspecific hybrids. Genetic diversity, measured as the average distances (expected heterozygosity) between individuals in the same cluster, was higher in Cerasus (0.78) followed by Prunus (0.72) and Amygdalus (0.64). Total number of alleles observed was 133, of which 14, 33, and 35 from six, 10, and 10 loci were unique for the peach, plum, and cherry rootstock groups, respectively. Alleles shared among peach/plum, plum/cherry, and peach/cherry rootstock genotypes were 13, 14, and 18 from nine, seven, and seven loci, respectively. Only six alleles from five loci were common to the three taxonomic groups. In addition, to develop a rootstock identification system based on SSR markers, a minimum set of three markers (PMS-3, BPPCT-037, and BPPCT-036) able to differentiate the 26 genotypes was identified. This study is the first step toward establishing a stone fruit rootstock breeding program in Chile.

scholarly journals Genetic diversity of different late blight responsive Indian potato cultivars as revealed by SSR markers

2020 ◽  
Vol 80 (02) ◽  
Author(s):  
Virupaksh U. Patil ◽  
G. Vanishree ◽  
Leena Saikia ◽  
Vinay Bhardwaj ◽  
S. K. Chakrabarti
Keyword(s):  
Genetic Diversity ◽  
Cluster Analysis ◽  
Late Blight ◽  
Microsatellite Markers ◽  
Ssr Markers ◽  
Potato Cultivars ◽  
Indian Potato Cultivars ◽  
Moderately Resistant ◽  
Resistant Genotypes ◽  
Very High

Forty seven resistant and susceptible to late blight Indian potato cultivars were used to assess genetic diversity present among them. A set of 24 microsatellite markers applied which generated 193 alleles with an average of 7.04 per loci and 4.10 per genotype. Eight alleles were specific to resistant cultivars, whereas 11 were specific to susceptible. Genetic diversity among the moderately resistant genotypes was found to be higher than the susceptible and resistant ones. The cluster analysis grouped the cultivars into 2 main and 4 sub clusters with late blight resistant genotypes distributed across the clusters. The diverisity among the genotypes was very high; however, diversity within groups viz., resistant, moderately resistant and susceptible was found to be very low (6%). The findings of the study would be of great help to the breeders in selecting genotypes for developing improved varieties of potato.

scholarly journals Potato cultivar identification using molecular markers

2010 ◽  
Vol 45 (1) ◽  
pp. 110-113 ◽  
Author(s):  
Patricia Marchezi Rosa ◽  
Tatiana de Campos ◽  
Adna Cristina Barbosa de Sousa ◽  
Danilo Augusto Sforça ◽  
Gisele Abigail Montan Torres ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Microsatellite Markers ◽  
Cultivar Identification ◽  
Potato Cultivar ◽  
Polyacrylamide Gel ◽  
Potato Cultivars ◽  
Varietal Identification ◽  
Identification And Characterization

The objective of this work was to evaluate a set of microsatellite markers for varietal identification and characterization of the most widespread potato cultivars in Brazil. The DNA from 14 potato cultivars was genotyped using microsatellite markers and the alleles were scored in silver-stained polyacrylamide gel. Twenty-four microsatellite markers were evaluated, and only one locus was monomorphic. Based on band patterns, a set of two microsatellites that were able to identify and differentiate all examined cultivars was obtained.

scholarly journals Characterization of Medicago populations under cold acclimation by morphological traits and microsatellite (SSR) markers

2014 ◽  
Vol 13 (27) ◽  
pp. 2704-2714
Author(s):  
Nourredine Yahia ◽  
Fyad Lameche ◽  
Zohra Fatima ◽  
Nacer Bakhti ◽  
Philippe Barre
Keyword(s):  
Cold Acclimation ◽  
Ssr Markers ◽  
Morphological Traits

scholarly journals Genetic variability for coloured caryopses in common wheat varieties determined by microsatellite markers

2013 ◽  
Vol 49 (No. 3) ◽  
pp. 116-122 ◽  
Author(s):  
M. Musilová ◽  
V. Trojan ◽  
T. Vyhnánek ◽  
L. Havel
Keyword(s):  
Microsatellite Markers ◽  
Ssr Markers ◽  
Polymorphic Information Content ◽  
Wheat Breeding ◽  
Wheat Varieties ◽  
Breeding Programmes ◽  
New Gene ◽  
Yellow Coloration ◽  
Selection Of

Products made from wheat are the most important components of the human diet, and could also become a source of functional foods and feed ingredients, e.g. minerals, vitamins and/or phytochemicals. The caryopses of certain wheat genotypes contain antioxidants, i.e. anthocyanins or carotenoids, which cause purple, blue or yellow coloration. The first step before the introduction of these traits into individual wheat cultivars is the characterization of relationships and the possibility of new gene combinations. In this study, relationships among 24 genotypes with different types of caryopsis colour were investigated by means of microsatellite markers. Using 44 SSR (Simple Sequence Repeat) markers it was possible to detect a total of 184 alleles; on average, approximately 4 alleles were detected at a microsatellite locus. Using a set of 5 SSR markers (Xgwm636, Xbarc077, Xwmc262, Xgwm397 and Xwmc219) with PIC (polymorphic information content) values higher than 0.70, it was possible to differentiate among all the genotypes analysed. A dendrogram was created on the basis of all SSR markers, and showed that the genotypes were divided into two groups. Three, and one genotype with purple and blue caryopsis, respectively, belonged to one cluster, while the remaining twenty formed the second, greater cluster, which was subdivided into 2 sub-clusters: one of them involved genotypes with blue caryopses, and the other those with yellow and red caryopses. The genotype of tall wheatgrass (Thinopyrum ponticum), as a possible donor of genes responsible for blue caryopses, was also classified. These results can be used in wheat breeding programmes aimed at the selection of functional foodstuffs.

scholarly journals The response of potato (Solanum tuberosum L) to the fungus Rhizoctonia solani

2010 ◽  
Vol 63 ◽  
pp. 277-277
Author(s):  
S.V. Raikar ◽  
F.A. Shah ◽  
A.R. Pitman ◽  
J.M.E. Jacobs
Keyword(s):  
Rhizoctonia Solani ◽  
Potato Breeding ◽  
Block Design ◽  
Stem Canker ◽  
Economic Loss ◽  
Black Scurf ◽  
Potato Cultivars ◽  
Potato Plants ◽  
Food Research ◽  
Potato Breeding Programme

Rhizoctonia solani is a soilborne fungal pathogen of many economically important crops Thirteen closely related anastomosis groups (AGs) of this pathogen have been identified each of which affects different plant species Previous studies have suggested that R solani AG3 is the main cause of disease in potato Rhizoctonia solani infection of potato plants can result in stem canker and tubers with black scurf Affected plants give a reduced yield while affected tubers are considered to have reduced quality resulting in economic loss The current study investigated the response of different potato cultivars and lines to R solani AG3 Thirtytwo potato cultivars/lines many actively used in the Plant Food Research potato breeding programme were screened in a shadehouse pot trial The trial was laid out in an adapted resolvable block design to ensure uniform exposure to environmental factors Each cultivar had six replicates four with and two without AG3 inoculum Plants were grown to maturity and the resulting tubers were assessed for disease (black scurf) Preliminary results show that none of the lines/cultivars tested were resistant but degrees of susceptibility varied In this experiment stem canker was not observed in any of the lines/cultivars

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