Development of chloroplast microsatellite markers for identification of Glycyrrhiza species

2018 ◽  
Vol 17 (1) ◽  
pp. 95-98 ◽  
Author(s):  
Kyung Jun Lee ◽  
Sebastin Raveendar ◽  
Ji Seon Choi ◽  
Jinsu Gil ◽  
Jeong Hoon Lee ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Traditional Medicine ◽  
Chloroplast Genome ◽  
Diversity Indices ◽  
Rapid Identification ◽  
Shannon Index ◽  
Medicinal Herbs ◽  
Medicinal Herb ◽  
Chloroplast Microsatellite

AbstractLicorice (Glycyrrhiza glabra) is an important medicinal herb and has long been used in traditional medicine for the treatment of several diseases worldwide. Understanding the genetic diversity within Glycyrrhiza species is important for the efficient conservation of these medicinal herbs. In this study, we have developed 20 polymorphic chloroplast microsatellite (cpSSR) markers using the chloroplast genome of G. lepidota. The cpSSR markers were tested on a total of 27 Glycyrrhiza individual plants. The number of alleles per locus ranged from two to eight among the Glycyrrhiza accessions. Overall, the Shannon index (I) for each cpSSR ranged from 0.315 to 1.694, the diversity indices (h) were 0.140–0.793 and the unbiased diversity indices (uh) were 0.145–0.825. In addition, the cpSSR markers were successfully divided and classified the 27 Glycyrrhiza individuals into four groups. The cpSSR markers developed in this study could be used in the assessment of genetic diversity and rapid identification of Glycyrrhiza species.

scholarly journals Evaluation of genetic variability among “Early Mature”Juglans regiausing microsatellite markers and morphological traits

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e3834 ◽  
Author(s):  
Aziz Ebrahimi ◽  
Abdolkarim Zarei ◽  
Mojtaba Zamani Fardadonbeh ◽  
Shaneka Lawson
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Normal Growth ◽  
Diversity Indices ◽  
Shannon Index ◽  
Phenotypic Trait ◽  
Phenotypic Traits ◽  
High Genetic Diversity ◽  
Early Maturation ◽  
Persian Walnut

Limiting the juvenile phase and reducing tree size are the two main challenges for breeders to improve most fruit crops. Early maturation and dwarf cultivars have been reported for many fruit species. “Early mature” and low vigor walnut genotypes were found among seedlings of Persian walnut. Nine microsatellite markers were used to evaluate genetic diversity among “Early Mature” Persian walnut accessions and provide a comparison with “normal growth” accessions. Six maturation related characteristics were also measured in “Early Mature” samples. Phenotypic traits and diversity indices showed relatively high levels of genetic diversity in “Early Mature” seedlings and indicated high differentiation between individuals. Seedling height, the most diverse phenotypic trait, has an important role in the clustering of “Early Mature” accessions. The “Early Mature” type had higher number of alleles, number of effective allele, and Shannon index compared to the “Normal Growth” group. The two types of studied walnuts had different alleles, with more than half of produced alleles specific to a specific group. “Early Mature” and “Normal Growth” walnuts had 27 and 17 private alleles, respectively. Grouping with different methods separated “Early Mature” and “Normal Growth” samples entirely. The presence of moderate to high genetic diversity in “Early Mature” walnuts and high genetic differentiation with “Normal Growth” walnuts, indicated that “Early Mature” walnuts were more diverse and distinct from “Normal Growth” samples. Moreover, our results showed SSR markers were useful for differentiating between “Early Mature” and “Normal Growth” walnuts. A number of identified loci have potential in breeding programs for identification of “Early Mature” walnuts at the germination phase.

Analysis of Genetic Diversity of Saccharum Complex Using Chloroplast Microsatellite Markers

Sugar Tech ◽  
2015 ◽  
Vol 18 (2) ◽  
pp. 141-148 ◽  
Author(s):  
Punnya Raj ◽  
A. Selvi ◽  
P. T. Prathima ◽  
N. V. Nair
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Saccharum Complex ◽  
Chloroplast Microsatellite

Nuclear and chloroplast microsatellite markers to assess genetic diversity and evolution in hazelnut species, hybrids and cultivars

2012 ◽  
Vol 60 (2) ◽  
pp. 543-568 ◽  
Author(s):  
Nahla Bassil ◽  
Paolo Boccacci ◽  
Roberto Botta ◽  
Joseph Postman ◽  
Shawn Mehlenbacher
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Chloroplast Microsatellite ◽  
Species Hybrids

scholarly journals Using microsatellite markers to analyze genetic diversity in 14 sheep types in Iran

2017 ◽  
Vol 60 (3) ◽  
pp. 183-189 ◽  
Author(s):  
Mohammad Taghi Vajed Ebrahimi ◽  
Mohammadreza Mohammadabadi ◽  
Ali Esmailizadeh
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Information Content ◽  
Gene Diversity ◽  
Polymorphic Information Content ◽  
Extraction Procedure ◽  
Shannon Index ◽  
Group Method ◽  
Salting Out ◽  
Pair Group

Abstract. Investigation of genetic relationship among populations has been traditionally based on the analysis of allele frequencies at different loci. The prime objective of this research was to measure the genetic polymorphism of five microsatellite markers (McMA2, BM6444, McMA26, HSC, and OarHH35) and study genetic diversity of 14 sheep types in Iran. Genomic DNA was extracted from blood samples of 565 individuals using an optimized salting-out DNA extraction procedure. The polymerase chain reaction (PCR) was successfully performed with the specific primers. Some locus–population combinations were not at Hardy–Weinberg equilibrium (P < 0. 05). The microsatellite analysis revealed high allelic and gene diversity in all 14 breeds. Pakistani and Arabi breeds showed the highest mean number of alleles (11.8 and 11 respectively), while the highest value for polymorphic information content was observed for the Arabi breed (0.88). A UPGMA (unweighted pair group method with arithmetic mean) dendrogram based on the Nei's standard genetic distance among studied breeds showed a separate cluster for Arabi and Pakistani breeds and another cluster for other breeds. The Shannon index (H0) for McMA2, BM6444, McMA26, HSC, and OarHH35 was 2.31, 2.17, 2.27, 2.04 and 2.18, respectively, and polymorphic information content (PIC) values were 0.88, 0.92, 0.87, 0.84, and 0.86 for McMA2, BM6444, McMA26, HSC, and OarHH35, respectively. The high degree of variability demonstrated within the studied sheep types implies that these populations are rich reservoirs of genetic diversity that must be preserved.

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