Laboratory Evaluation of Piper guineense for the Protection of Cowpea Against Callosobruchus maculatus

1988 ◽  
Vol 9 (01) ◽  
pp. 55-59 ◽  
Author(s):  
Julius I. Olaifa ◽  
Wilson O. Erhun
Keyword(s):  
Callosobruchus Maculatus ◽  
Laboratory Evaluation ◽  
Piper Guineense

scholarly journals Perforation index assessment of cowpea seeds against cowpea bruchid, Callosobruchus maculatus (Fabricius) [Coleoptera: Chrysomelidae], infestation using Piper guineense

2020 ◽  
Vol 81 (1) ◽  
Author(s):  
Kayode David Ileke ◽  
Jacobs Mobolade Adesina ◽  
Luke Chinaru Nwosu ◽  
Abimbola Olagunju
Keyword(s):  
Callosobruchus Maculatus ◽  
Adult Emergence ◽  
Adult Mortality ◽  
Contact Toxicity ◽  
Toxicity Assay ◽  
Piper Guineense ◽  
Cowpea Seeds ◽  
Leaf Powder ◽  
Human Use ◽  
Seed Extracts

Abstract Background Powders and extracts of Piper guineense seeds and leaves were assessed for insecticidal activities against Callosobruchus maculatus in the laboratory at temperature and relative humidity of 29.6 °C and 75.9%, respectively. Bioactive compounds in P. guineense leaves and seeds were also investigated. The powders were tested at rates 1.0, 2.0 and 4.0 g/20 g cowpea seeds while extracts were tested at 1.0, 2.0 and 3.0%. Results Results of contact toxicity assay of the seed powder caused 100% adult mortality at 96 h post-treatment period whereas leaf powder evoked 90% adult mortality within the same period at concentration of 1.0 g/20 g cowpea seeds. Low adult emergence was observed on cowpea seeds treated with 1 g of seed powder with percentage adult emergence of 10.0% and inhibition rate (IR) of 97.5%. Beetle Perforation Index (BPI) obtained from treated cowpea seeds was significantly different (P < 0.05) from BPI of untreated seeds. Extracts of P. guineense seed were more toxic than seed powder. Piper guineense seed extract caused 87.5% adult mortality of C. maculatus while leaf extract caused 70.0% adult mortality within 24 h of infestation at concentration of 1%. Progeny development of C. maculatus was completely inhibited in cowpea treated with 2% and 3% leaf and seed extracts of P. guineense. β-Pinene was the most abundant active compound in P. guineense seed (55.6%) and leaf (48.4%). β-Phellandrene occurred 38.2% in seeds while Ocimene had the least value of 0.2% in seed and 0.5% in leaf. Conclusion The study showed that P. guineense seed powder and extracts were more effective than leaf powder and extract. Utilization of plant products as alternative to synthetic insecticides in protecting cowpea seeds against C. maculatus should be encouraged for enhanced food safety and security. Piper guineense is used as spice and medicine and interestingly safe for human use.

scholarly journals Callosobruchus maculatus (Fab.) control by plant products in cowpea grains under storage: A review

2017 ◽  
Vol 1 ◽  
pp. 51 ◽  
Author(s):  
A. Muhammad ◽  
A. K. Bashir
Keyword(s):  
Callosobruchus Maculatus ◽  
Groundnut Oil ◽  
Plant Materials ◽  
Piper Guineense ◽  
Plant Products ◽  
Germination Process ◽  
Leaf Powder ◽  
Cowpea Seed ◽  
The Tropics ◽  
Better Than

The present review focuses on some major researches dealing with controlling Cowpea seed bruchid (CSB) due to Callosobruchus maculatus (Fab.) [Coleoptera: Bruchidae] by using some plant products. The objective of this review is to look in to the successes of the use of plant materials in the management of CSB especially in the tropics where bulk of the crop is cultivated and consumed. The review of available literature showed that, plants such as Neem, Azadirachta indica A. Juss; Garlic, Allium sativum (L.); West African pepper, Piper guineense Schumach; drum stick, Moringa oleifera Lam; African Basil, Ocimum gratissimum (L.); Moss plant; Barbula indica and Clausena anisata (Willd.) Hook has been used in CSB control. Available literatures showed that garlic, chilies and peppermint applied at the rate of 0.035–0.55g significantly (p≤0.05) reduced oviposition, respectively compared to the control. Similarly, powdered flowers of M. oleifera applied at the rate of 0.5 g per 30 g of seeds caused mortality of CSB better than the control 8 hours after infestation. The use of C. anisata and Permethrin showed percentage mortality of cowpea bruchids was high using Permethrin but was not significantly (p≥0.05) better than Clausena leaf powder. Groundnut oil applied at ˂ 4mls kg-1 does not affect germination process of cowpea stored for up to 12 weeks. However, increasing rate of application decreases germination. The review clearly indicated that plant products have potentials of controlling CSB in stored cowpea as they are safe and free from residue. They are hereby encouraged.

scholarly journals Efficacy of Piper guineense Schumach. seed powder in the control of Callosobruchus maculatus (Fabricius, 1775) (Coleoptera: Chrysomelidae: Bruchinae) and on the nutritional and organoleptic characteristics of stored cowpea Vigna unguiculata (L.) Walp.

2018 ◽  
Vol 87 (2) ◽  
pp. 119-140
Author(s):  
Frank O. Ojiako ◽  
Adeyinka A. Adesiyun ◽  
Gabriel O. Ihejirika ◽  
Sunday A. Dialoke
Keyword(s):  
Callosobruchus Maculatus ◽  
Adult Emergence ◽  
Adult Mortality ◽  
Proximate Analysis ◽  
Crude Fibre ◽  
Piper Guineense ◽  
Seed Damage ◽  
Cowpea Seeds ◽  
Organoleptic Characteristics ◽  
Cowpea Bruchid

Abstract Brown pepper (Piper guineense) seed powder, used for culinary and medicinal purposes, was evaluated in the laboratory with a conventional storage chemical, actellic 2% dust (Pirimiphos-methyl), as standard, to protect stored cowpea against the cowpea bruchid, Callosobruchus maculatus. Four rates of the seed powder were used (2.5, 5.0 and 10.0 g/100 g seed with a control, 0.0 g) while actellic dust was applied at 1.0, 2.0, 3.0 g/100 g seed and 0.0 g/100 g seed as control. Data on percentage mortality, oviposition, adult emergence and seed damage were collected over a six-month period. Weevil perforation indexes (WPI) were estimated with the seed damage data. All data were analysed for variance at P≤0.05. Actellic dust at the lowest dose inflicted adult mortality (90 – 100%) in the first 48 h after treatment and prevented oviposition (95 – 100%) in the first five months of storage compared to the control. The highest rate of Piper guineense application caused mortality of up to 90.0% in 48 h, reduced oviposition (70 – 80%), decreased adult emergence (15.90 to 28.40 insects) as against the control (104.7 – 272.7 insects) and reduced seed damage by 80 % with a WPI of between 33.3 and 10.0 %. Proximate analysis and germination tests indicated that the treatment materials increased the moisture and crude fibre content, decreased crude protein content but had no significant effect on the viability of stored seeds. Organoleptic tests demonstrated that treatment materials had no significant negative effect on taste, odour, texture, appearance and overall acceptability of cooked cowpea seeds after six months of storage. It can be safely concluded that the powdered form of P. guineense seed at the highest doses (5.0 and 10.0 g) gave significant protection of cowpea seeds against the cowpea bruchid, which compared favourably with actellic dust when applied topically.

LABORATORY EVALUATION OF BIOLOGICAL ACTIVITY OF CINNAMOMUM CASSIA TO FOUR SPECIES OF STORED-PRODUCT INSECTS1

1985 ◽  
Vol 20 (2) ◽  
pp. 247-253 ◽  
Author(s):  
Helen C. F. Su
Keyword(s):  
Biological Activity ◽  
Callosobruchus Maculatus ◽  
Sitophilus Oryzae ◽  
Acetone Extract ◽  
Laboratory Evaluation ◽  
Contact Toxicity ◽  
Lasioderma Serricorne ◽  
Class Iii ◽  
Cinnamomum Cassia ◽  
Application Rates

Dry bark and oil of Cinnamomum cassia Blume were evaluated for their biological activity against the adults of Callosobruchus maculatus (F.), Sitophilus oryzae (L.), Lasioderma serricorne (F.) and Tribolium confusum Jacquelin du Val. The acetone extract of the bark provided practically no contact toxicity to C. maculatus and T. confusum up to 50 μg/insect, but gave weak toxicity to S. oryzae and L. serricorne at 48 h after topical treatment, while the oil showed no toxicity to C. maculatus, very weak toxicity to T. confusum, and moderate toxicity to S. oryzae and L. serricorne at 30 and 50 μg/insect, respectively. Both the extract and the oil, applied to wheat at 0.2, 0.1 and 0.05% by weight, showed significant repellency against S. oryzae adults with the oil providing greater repellency than the extract. The repellency decreased as the concentration of treatment decreased. Both the extract and the oil provided the repellency to T. confusum when applied to paper. At the application rates of 600 and 400 μg/cm2, the extract showed a class III average repellency for 4 months. Repellency of the oil deteriorated at a faster rate, and showed only a class II average repellency for 4 months at the same dosages.

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