Comparison of bovine rumen liquor and faeces as sources of micro-organisms for the in vitro gas production technique assessed using twelve graminaceous forages

1998 ◽  
Vol 1998 ◽  
pp. 68-68
Author(s):  
R. Mauricio ◽  
A.L. Abdalla ◽  
F.L. Mould ◽  
U.R. Altaf ◽  
T. Smith ◽  
...  
Keyword(s):  
Pressure Transducer ◽  
Gas Production ◽  
Production Technique ◽  
Bovine Rumen ◽  
In Vitro Gas Production ◽  
Micro Organisms

The experiment was conducted using a range of forages with accurately predetermined OMD values (ADAS) to compare rumen liquor (RL) and faeces (FA) as sources of inocula in the pressure transducer technique (PTT) (Theodorou et al., 1994). Gas production results were examined in relation to OMD determined in vitro (PTT, Tilley and Terry) and in vivo.

Comparison of bovine rumen liquor and faeces as sources of micro-organisms for the in vitro gas production technique assessed using twelve graminaceous forages

1998 ◽  
Vol 1998 ◽  
pp. 68-68 ◽  
Author(s):  
R. Mauricio ◽  
A.L. Abdalla ◽  
F.L. Mould ◽  
U.R. Altaf ◽  
T. Smith ◽  
...  
Keyword(s):  
Pressure Transducer ◽  
Gas Production ◽  
Production Technique ◽  
Bovine Rumen ◽  
In Vitro Gas Production ◽  
Micro Organisms

The experiment was conducted using a range of forages with accurately predetermined OMD values (ADAS) to compare rumen liquor (RL) and faeces (FA) as sources of inocula in the pressure transducer technique (PTT) (Theodorou et al., 1994). Gas production results were examined in relation to OMD determined in vitro (PTT, Tilley and Terry) and in vivo.

Comparison of bovine rumen liquor and bovine faeces as sources of micro-organisms for the in vitro gas production technique for assessing silages of maize and maize plant fractions

1998 ◽  
Vol 1998 ◽  
pp. 60-60
Author(s):  
U.R. Altaf ◽  
R. Mauricio ◽  
F.L. Mould ◽  
T. Smith ◽  
E. Owen ◽  
...  
Keyword(s):  
Organic Matter ◽  
Gas Production ◽  
Maize Plant ◽  
Production Technique ◽  
Maize Silage ◽  
Bovine Rumen ◽  
In Vitro Gas Production ◽  
Organic Matter Digestibility ◽  
Micro Organisms

A previous study (Mauricio et al., 1998) with 12 forage substrates (straw, hay and dried grasses) showed a high correlation between rumen liquor and faeces for total gas production and in vitro organic matter digestibility (OMD). However parameters estimated using faecal inoculum were generally lower man when using rumen liquor. To confirm this observation, a second study was conducted using maize silage and silages made from maize plant fractions.

Relationship between in vitro gas production and in vivo energy digestibility in growing pigs

1996 ◽  
Vol 1996 ◽  
pp. 142-142
Author(s):  
A.C. Longland ◽  
S.P. Bray ◽  
A.E. Brooks ◽  
M.K. Theodorou ◽  
A.G. Low
Keyword(s):  
Pressure Transducer ◽  
Nutritive Value ◽  
Rumen Fluid ◽  
Gas Production ◽  
Growing Pigs ◽  
Digestible Energy ◽  
In Vitro Gas Production

In vitro methods which can be used to predict the nutritive value of feedstuflfs for livestock are attractive in terms of both speed and economy. The in vitro pressure transducer technique (PTT) whereby the gas evolved during the in vitro fermentation of feedstuffs in rumen fluid is quantified by a pressure transducer, has been used to predict the nutritive value of ruminant feedstuffs. Here the potential for the PTT in predicting the digestible energy contents of eight feedstuffs varying in non-starch polysaccharide (NSP) content fed to growing pigs, was investigated.

Comparison of rumen liquor and faeces, in UK and Brazil, as sources of microorganisms for in vitro gas production for assessing twelve forages

1999 ◽  
Vol 1999 ◽  
pp. 147-147 ◽  
Author(s):  
R. M. Mauricio ◽  
E. Owen ◽  
A.L Abdalla ◽  
I.C.S. Bueno ◽  
F. L. Mould ◽  
...  
Keyword(s):  
Pressure Transducer ◽  
Tropical Climate ◽  
Gas Production ◽  
Tropical Environment ◽  
Lag Phase ◽  
Production Technique ◽  
Grass Silage ◽  
In Vitro Gas Production ◽  
Reading Study

In a previous study in Reading (altitude 66 m) (Mauricio et al., 1997) the lag phase was greater when cow faeces was used as a source of microorganisms in the in vitro gas production technique instead of rumen liquor when twelve temperate forages were fermented for 96 h. In the Reading study faeces and rumen liquor were obtained from a cow fed grass silage and concentrate (60:40). The present study was done in Piracicaba, Brazil-BR (altitude 780 m) which has a tropical climate. Using the same forages as in Mauricio et al. (1998), the study examined whether the same differences between faeces and rumen liquor would occur in a tropical environment. In addition, the opportunity was taken to develop an equation relating pressure and volume for the semi-automated pressure transducer technique and compare it with the equation developed in UK by Mauricio et al. (1998).

Comparison of bovine rumen liquor and bovine faeces as sources of micro-organisms for thein vitrogas production technique for assessing silages of maize and maize plant fractions

1998 ◽  
Vol 1998 ◽  
pp. 60-60 ◽  
Author(s):  
U.R. Altaf ◽  
R. Mauricio ◽  
F.L. Mould ◽  
T. Smith ◽  
E. Owen ◽  
...  
Keyword(s):  
Organic Matter ◽  
Gas Production ◽  
Maize Plant ◽  
Production Technique ◽  
Maize Silage ◽  
Bovine Rumen ◽  
Organic Matter Digestibility ◽  
Micro Organisms

A previous study (Mauricioet al., 1998) with 12 forage substrates (straw, hay and dried grasses) showed a high correlation between rumen liquor and faeces for total gas production andin vitroorganic matter digestibility (OMD). However parameters estimated using faecal inoculum were generally lower man when using rumen liquor. To confirm this observation, a second study was conducted using maize silage and silages made from maize plant fractions.

Relationship between in vitro gas production and in vivo energy digestibility in growing pigs

1996 ◽  
Vol 1996 ◽  
pp. 142-142
Author(s):  
A.C. Longland ◽  
S.P. Bray ◽  
A.E. Brooks ◽  
M.K. Theodorou ◽  
A.G. Low
Keyword(s):  
Pressure Transducer ◽  
Nutritive Value ◽  
Rumen Fluid ◽  
Gas Production ◽  
Growing Pigs ◽  
Digestible Energy ◽  
In Vitro Gas Production

In vitro methods which can be used to predict the nutritive value of feedstuflfs for livestock are attractive in terms of both speed and economy. The in vitro pressure transducer technique (PTT) whereby the gas evolved during the in vitro fermentation of feedstuffs in rumen fluid is quantified by a pressure transducer, has been used to predict the nutritive value of ruminant feedstuffs. Here the potential for the PTT in predicting the digestible energy contents of eight feedstuffs varying in non-starch polysaccharide (NSP) content fed to growing pigs, was investigated.

The prediction of in vivo methane production and animal performance from the in vitro gas production technique

2003 ◽  
Vol 2003 ◽  
pp. 157-157
Author(s):  
A. Bortolozzo ◽  
D. K. Lovett ◽  
S. Lovell ◽  
L. Stack ◽  
F. P. O’Mara
Keyword(s):  
Chemical Composition ◽  
Methane Production ◽  
Gas Production ◽  
Animal Performance ◽  
Production Technique ◽  
In Vitro Gas Production ◽  
Ad Libitum

The in vivo determination of methane (CH4) production requires specialist equipment which is costly to maintain. Whilst the in vitro gas production technique has been demonstrated to show potential to rank diets for their methanongenic potential at maintenance planes of nutrition (Moss and Givens, 1997) no study has investigated this relationship when feedstuffs are fed ad libitum. The objective of this study was to assess the ability of the technique to predict in vivo CH4 production and animal performance from six diets differing in their chemical composition.

Factors affecting the amount of indirect gas produced by the in vitro gas production technique

1998 ◽  
Vol 22 ◽  
pp. 89-91 ◽  
Author(s):  
C. Rymer ◽  
A. R. Moss ◽  
E. R. Deaville ◽  
D. I. Givens
Keyword(s):  
Carbon Dioxide ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Gas Production ◽  
Production Technique ◽  
Factors Affecting ◽  
Bicarbonate Ions ◽  
In Vitro Gas Production ◽  
Micro Organisms

When a food is ingested by a ruminant animal, the carbohydrate fraction of the food is fermented by the rumen micro-organisms to produce gas (predominantly carbon dioxide and methane) as well as volatile fatty acids (VFA). The gas production technique simulates this fermentation process and provides an estimate of both the rate and extent of fermentation. Comparing the gas production (GP) profiles of foods enables a comparison to be made of the fermentative characteristics of different foods. However, the technique uses a bicarbonate-based medium system with the rumen liquor. This complicates the GP profile because of the production of ‘indirect’ gas resulting from the reaction between the VFA and the bicarbonate ions.Beuvink and Spoelstra (1992) measured the volume of gas produced from buffered rumen fluid when known amounts of VFA were added and observed that 20·8 ml gas were released per mmol VFA. However, there is variation between laboratories in terms of the composition of the medium that is used. Even when the same medium is used, significant differences have been observed in the GP profile when different types of apparatus were employed (Rymer and Givens, 1997). Media are gassed with carbon dioxide before they are added to the gas production system and it is possible that the concentration of carbon dioxide dissolved in the medium varies between experiments. The objective of this experiment, therefore, was to determine whether the volume of indirect gas produced was affected by the composition of the medium, the addition of carbon dioxide, and the technique employed to measure gas production.

Comparison of rumen liquor and faeces from cows as sources of micro-organisms for the in vitro gas production technique

1998 ◽  
Vol 22 ◽  
pp. 182-184 ◽  
Author(s):  
R. M. Mauricio ◽  
E. Owen ◽  
M. S. Dhanoa ◽  
M. K. Theodorou
Keyword(s):  
Present Experiment ◽  
Gas Production ◽  
Invasive Technique ◽  
Production Technique ◽  
Production Methods ◽  
Rumen Degradation ◽  
In Vitro Gas Production ◽  
Micro Organisms

Bioassay methods to estimate the digestibility of forages for ruminants, such as the in vitro digestibility technique (Tilley and Terry, 1963), the nylon bag technique (Ørskov et al., 1980) and the gas production methods of Menke and Steingass (1988) and Theodorou et al. (1994), require rumen fistulated animals, either to provide a suitable in situ environment or to provide rumen liquor as a source of inoculum. Not only is establishing and maintaining fistulated animals expensive, but fistulation is an invasive technique which is increasingly discouraged on animal welfare grounds. There is therefore a need to find an alternative to rumen liquor as a source of micro-organisms for bioassays.Although the Tilley and Terry (1963) technique is widely used, it is limited by being an end-point digestibility method. Ørskov et al. (1988) showed that intake of forages and their rate of digestion in the rumen are more correlated than intake and digestibility. Thus, since 1988, there has been much interest in determining rate of rumen degradability using the nylon bag technique (Huntington and Givens, 1995). However, as indicated earlier, this in vitro technique requires fistulated animals. Recently Sileshi et al. (1996) showed that the in vitro gas production technique of Theodorou et al. (1994), offers a possibility of assessing rate of rumen degradation.The purpose of the present experiment was to compare rumen liquor and faeces as sources of inoculum in the gas technique of Theodorou et al. (1994).

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