Comparison of rumen liquor and faeces, in UK and Brazil, as sources of microorganisms for in vitro gas production for assessing twelve forages

1999 ◽  
Vol 1999 ◽  
pp. 147-147 ◽  
Author(s):  
R. M. Mauricio ◽  
E. Owen ◽  
A.L Abdalla ◽  
I.C.S. Bueno ◽  
F. L. Mould ◽  
...  
Keyword(s):  
Pressure Transducer ◽  
Tropical Climate ◽  
Gas Production ◽  
Tropical Environment ◽  
Lag Phase ◽  
Production Technique ◽  
Grass Silage ◽  
In Vitro Gas Production ◽  
Reading Study

In a previous study in Reading (altitude 66 m) (Mauricio et al., 1997) the lag phase was greater when cow faeces was used as a source of microorganisms in the in vitro gas production technique instead of rumen liquor when twelve temperate forages were fermented for 96 h. In the Reading study faeces and rumen liquor were obtained from a cow fed grass silage and concentrate (60:40). The present study was done in Piracicaba, Brazil-BR (altitude 780 m) which has a tropical climate. Using the same forages as in Mauricio et al. (1998), the study examined whether the same differences between faeces and rumen liquor would occur in a tropical environment. In addition, the opportunity was taken to develop an equation relating pressure and volume for the semi-automated pressure transducer technique and compare it with the equation developed in UK by Mauricio et al. (1998).

Predicting the apparent disappearance of starch in the rumen of cows from the in vitro gas production profile of their diet

1999 ◽  
Vol 1999 ◽  
pp. 157-157 ◽  
Author(s):  
C. Rymer ◽  
D.I. Givens
Keyword(s):  
Latin Square ◽  
Gas Production ◽  
Holstein Cows ◽  
Production Technique ◽  
Grass Silage ◽  
Protein System ◽  
In Vitro Gas Production

Assessing the availability of a feed in the rumen is an important element of its characterisation. In the Metabolisable Protein system, it is assumed that all starch is available in the rumen, although clearly this is not the case. However, estimating the amount of starch that is apparently fermented in the rumen is difficult, expensive and cannot be routinely undertaken. The objective of this experiment was to determine whether the in vitro gas production technique could be used as a means of predicting the amount of starch that apparently disappears in the rumen (STA ADR).Four Holstein cows, each fitted with a rumen and duodenal cannula, were used. They were fed one of four diets in a 4x4 latin square design. The diets consisted of grass silage (GS), molassed sugarbeet feed (MSBF), fishmeal (FM) and minerals (MIN). They were also supplemented with either wheat (WHT) or maize (MZE).

Comparison of bovine rumen liquor and faeces as sources of micro-organisms for the in vitro gas production technique assessed using twelve graminaceous forages

1998 ◽  
Vol 1998 ◽  
pp. 68-68
Author(s):  
R. Mauricio ◽  
A.L. Abdalla ◽  
F.L. Mould ◽  
U.R. Altaf ◽  
T. Smith ◽  
...  
Keyword(s):  
Pressure Transducer ◽  
Gas Production ◽  
Production Technique ◽  
Bovine Rumen ◽  
In Vitro Gas Production ◽  
Micro Organisms

The experiment was conducted using a range of forages with accurately predetermined OMD values (ADAS) to compare rumen liquor (RL) and faeces (FA) as sources of inocula in the pressure transducer technique (PTT) (Theodorou et al., 1994). Gas production results were examined in relation to OMD determined in vitro (PTT, Tilley and Terry) and in vivo.

Comparison of bovine rumen liquor and faeces as sources of micro-organisms for the in vitro gas production technique assessed using twelve graminaceous forages

1998 ◽  
Vol 1998 ◽  
pp. 68-68 ◽  
Author(s):  
R. Mauricio ◽  
A.L. Abdalla ◽  
F.L. Mould ◽  
U.R. Altaf ◽  
T. Smith ◽  
...  
Keyword(s):  
Pressure Transducer ◽  
Gas Production ◽  
Production Technique ◽  
Bovine Rumen ◽  
In Vitro Gas Production ◽  
Micro Organisms

The experiment was conducted using a range of forages with accurately predetermined OMD values (ADAS) to compare rumen liquor (RL) and faeces (FA) as sources of inocula in the pressure transducer technique (PTT) (Theodorou et al., 1994). Gas production results were examined in relation to OMD determined in vitro (PTT, Tilley and Terry) and in vivo.

Estimation of fermentable energy by the automatic in vitro gas production technique for a selection of feedstuffs incubated alone and in combination

1998 ◽  
Vol 1998 ◽  
pp. 69-69
Author(s):  
S. Fakhri ◽  
A. R. Moss ◽  
D.I. Givens ◽  
E. Owen
Keyword(s):  
Organic Matter ◽  
Incubation Period ◽  
Gas Production ◽  
Production Technique ◽  
In Vitro Gas Production ◽  
Fe Content ◽  
The Individual ◽  
Gas Volume ◽  
Selection Of

The gas production (GP) technique has previously been used to estimate the gas volume (fermentable energy (FE)) of compound feed ingredients for ruminants (Newbold et al., 1996). It was shown that the FE content of feed mixtures was represented by the combination of the total gas from the incubation of the individual feeds. However this additivity might not be consistent throughout the incubation period. The objectives were to test whether 1. other GP parameters give better estimates of FE for simple mixtures and are they additive; 2. whether organic matter apparently degraded in the rumen (OMADR) explain differences in GP; and 3. to find out if there are any other better measures than OMADR for estimating FE.

Pre-incubation of microbes for use in the Pressure Transducer Technique (PTT) of feed evaluation - effect of basal feed composition

1996 ◽  
Vol 1996 ◽  
pp. 220-220
Author(s):  
D M Harris
Keyword(s):  
Pressure Transducer ◽  
Phase 1 ◽  
Barley Grain ◽  
Gas Production ◽  
Lag Phase ◽  
Feed Composition ◽  
Grass Silage ◽  
High Starch ◽  
Effect Of The Composition ◽  
High Cellulose

The use of the Pressure Transducer Technique (PTT) to evaluate feed degradation as described by Theodorou et al (1994) recognises the presence of a lag phase (1-2h) after introducing the microbial innocula to the feed under investigation. Previous work has shown that this can be reduced by incubating the microbes with a basal feed for 24h prior to the addition of a test feed. This work considered the effect of the composition of this basal feed.Four basal mixtures, Grass silage/conc(G), Hay/cone (H), Maize silage/conc: (M), Whole crop wheat/caustic wheat/cone (W) were prepared plus two mixtures based on purified nutrients to give high Starch (St) and high Cellulose (Ce) contents. 20ml strained, homogenised bovine rumen liquor was added to bottles containing 180ml of media and 1.5g DM of each basal mixture. After 24h of vented incubation at 39°C 1g DM of test feed, (either barley grain or straw both milled to 1 mm) was added and gas production measured at decreasing frequencies over the next 72h. Cumulative gas production at 72h were calculated (Table 1).

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