Addition of Lactic Acid Bacteria Can Promote the Quality and Feeding Value of Broussonetia papyrifera (Paper Mulberry) Silage

In this study, the influence of two lactic acid bacteria (LAB) strains [Lactobacillus rhamnosus BDy (LR-BDy) and Lactobacillus buchneri TSy (LB-TSy)] selected from Southwest China on the fermentation characteristics and in vitro gas production of Broussonetia papyrifera (paper mulberry) silage were experimentally explored. The experimental groups were a control group (C), an LB-TSy treatment (LB), an LR-BDy treatment (LR), and an LR-BDy + LB-TSy hybrid group (LR × LB). After the LAB were added, the pH value of paper mulberry silage significantly declined (p < 0.05), and the crude protein content was effectively preserved (p < 0.05). However, no significant changes were found in the levels of neutral detergent fiber, acid detergent fiber, and crude ash (p > 0.05). The lactic acid content in paper mulberry silage was evidently increased (p < 0.05). The in vitro gas production in the LR at 36, 48, and 72 h were markedly higher than that in the other treatments (p < 0.05). Owing to the addition of LAB, the microbial diversity in paper mulberry silage was reduced, while the relative bacterial abundance of Lactobacillus was enhanced. Hence, the addition of LAB selected from the warm and humid region in Southwest China can improve the quality of paper mulberry silage and elevate its feeding value in this region.

Effect of fermentation on mixed rumen contents and jackfruit peel using Aspergillus oryzae on in vitro gas production and digestibility

The objective of this research was to investigate the effect of Aspergillus oryzae on fermentation of mixture of rumen contents and jackfruit peel on in vitro gas production and digestibility. The method used in this study was an experiment using a randomized block design (RBD) of 4 treatments and 3 replications. The treatments including of T0 = 50% rumen contents + 50% jackfruit peel, T1 = 40 % rumen contents + 60% jackfruit peel + 0,4% Aspergillus oryzae, T2 = 30% rumen contents + 70% jackfruit peel + 0,4% Aspergillus oryzae, T3 = 20% rumen contents + 80% jackfruit peel + 0,4% Aspergillus oryzae. Variables observed were gas production, dry matter digestibility (DMD), and organic matter digestibility (OMD). Data were analyzed by using Analysis of Variance (ANOVA) from Randomized Block Design, if there were significant effect between the treatments then tested with least significant different (LSD). The result showed that fermentation of mixture of rumen contents and jackfruit peel have highly significant effect (P<0.01) on gas production, and significant effect (P<0.05) on DMD and OMD. Gas production, DMD and OMD of fermented mixture of rumen contents and jackfruit peel are higher than control without fermentation. It is concluded that the higher jackfruit peels the higher gas production, DMD and OMD.

Producción de metano in vitro y parámetros fermentativos de mezclas de ensilado de girasol silvestre y pasto elefante, inoculadas o no con cepas de bacterias ácido-lácticas epífitas

The present investigation was carried out to determine the extent of the incorporation of Tithonia diversifolia (TD) and the possibility of blending it with Pennisetum purpureum (PP) to obtain the maximum benefit for ensilability and for animal nutrition. Silage mixtures of wild sunflower (TD) and elephant grass (PP) were evaluated based on chemical composition, quantification of gas production, methane release and fermentation parameters. The silage blends were arranged in four T. diversifolia / P. purpureum proportions, namely: 100/0; 67/33; 33/67; and 0/100 (fresh weight). Silages with higher proportions of T. diversifolia increased crude protein content, in vitro digestibility while decreasing NDF and ADF fractions (P<0.05). High amounts of T. diversifolia showed the lowest gas production values (160.2 ml), while treatments with higher grass inclusion produced a greater amount of gas up to 194.5 ml. Methane production was higher by increasing the proportion of P. purpureum into the silage blends. The silage inoculum did not have any impact on in vitro gas production (P<0.05). Also, higher proportions of T. diversifolia reduced acidification process while P. purpureum inclusion facilitated lower pH values. Lactic acid bacteria inoculum tended to decrease pH of silages but no clear effects on silage temperature were observed. Silages with high proportions of T. diversifolia (67 % of inclusion) would be more palatable for animals and might also translate into larger animal performance due to greater protein supply and better digestibility than silages with larger proportion of P. purpureum (67 and 100 % of inclusion).

Kinetics of In Vitro Gas Production and Fitting Mathematical Models of Corn Silage

This study aimed at examining the effects of rumen inoculum of steers receiving different combinations of ionophore and probiotics in their diets on in vitro gas production of corn silage. The fitting of gas production was performed with five mathematical models and its kinetics was evaluated. Four crossbred steers (403.0 ± 75.5 kg body weight) with ruminal cannula were assigned to a 4 × 4 Latin square design. The additives used were Monensin sodium (Rumensin® 100, 3 g/day), Bacillus toyonensis (Micro-Cell Platinum® 109, 1 g/day) and Saccharomyces cerevisiae boulardii (ProTernative®20, 0.5 g/day). Additives were arranged into the following treatments, supplied daily into total mixed diet: (1) Monensin; (2) Monensin + B. toyonensis; (3) Monensin + S. boulardii; and (4) B. toyonensis + S. boulardii. The gas production data were fitted into the models of Gompertz, Groot, Ørskov, Brody, Richards, and Dual-pool Logistic. A perfect agreement between observed and predicted values in curves of accumulated in vitro gas production was observed in the Groot and Richards models, with higher coefficient of determination (R2 = 0.770 and 0.771, respectively), concordance correlation coefficient (CCC = 0.871 and 0.870, respectively), and root mean square error of prediction (RMSEP = 1.14 and 1.15, respectively). Evaluating the feed additives throughout the Groot model, the B. toyonensis + S. boulardii treatment presented higher VF (12.08 mL/100 mg of DM; p = 0.0022) than Monensin and Monensin + S. boulardii (9.16 and 9.22 mL/100 mg of DM, respectively). In addition, the fractional rate of gas production (k) was higher (p = 0.0193) in B. toyonensis + S. boulardii than in Monensin, not presenting a statistical difference (p > 0.05) from the other two treatments. Additionally, with the time of beginning to gas production, the lag time (λ), was greater (p < 0.001) with Monensin and Monensin + B. toyonensis than with Monensin + S. boulardii and B. toyonensis + S. boulardii. The combination of Monensin and probiotics (B. toyonensis + S. boulardii) resulted in better kinetics of degradation of corn silage, being that the Groot and Richards models had the best fit for estimates of the in vitro gas production data of corn silage tested with different feed additive combinations.

Comparison of three different rumen fluid as a source of inoculum to evaluate in vitro gas production and digestibility of elephant grass-concentrate mixture

This study aimed at elucidating the use of three different rumen fluid (RF) of indigenous cattle breeds i.e. Bali, Madura and Crossbred Ongole immediately after slaughtered at abattoir to evaluate the nutritive value of elephant grass( EG) -concentrate mixture using a standard in vitro gas production (IVGP) technique. Approximately 500 mg feed dry matter/syringe was added with 50 ml RF-buffer solution and incubated in a 39 0C water bath for 48 hours where gas production was observed at time intervals. Following termination of incubation the content was transferred into tare glass crucible to measure rumen dry matter (RDMD) and organic matter (ROMD) digestibility. The results showed that there was no significant different (P>0.05) in gas production parameters. In contrast, RDMD and ROMD differed significantly (P<0.01) among cattle breeds. RF from OCB resulted in the highest IVGP, RDMD and ROMD as compared with other RF sources. In conclusion, the use of RF from abattoir for IVGP measurement can be warranted using the same source of RF. The highest values resulted from OCB suggests that the abundance and variation in rumen microbiota may exist among cattle breeds.

In vitro gas production and cinetical fermentation of a few local feed in Kupang East Nusa Tenggara

Research objectives were to evaluate and to analyze in vitro gas production and cinetical fermentation of Kupang’s local feed. The feed materials were dried and grinded for nutrient and gas produced analysis. Collected data were chemical composition, in vitro gas production, cinetical fermentation, short chain fatty acids, energy metabolic, and organic matter digestation. The result showed that the materials have different gas production. Commonly, the higher the carbohydrate content the higher the gas production; the higher the protein and the fat content the lower the gas production. Total gas productions of each material after 96 hours incubation were 53.88 ml/0.5 g DM for the high fat content feed, 92.26 and 159.25 ml/0.5 DM for the high protein and high carbohydrate content. Regarding total production, the fermented fraction gas productions were 44.662; 82.54 and 153.462 ml/0.5 g DM. The short chain fatty acids produced were 0.31; 0.51 and 1.28 mM. The feed organic matter digestations produced were 42.2; 55.91 and 68.8 % for each feed with a high content of fat, protein, and carbohydrate. Conclusively comparing with the protein and carbohydrate feed sources, the higher the fat content the lower the gas production, short chain fatty acids, and organic matter digestion.

Effect of using different levels of cassava meal in a concentrate cassava peel diet on chemical composition, in vitro gas production, and rumen fermentation

This study was designed to evaluate effects of using different levels of cassava meal in a concentrate cassava peel diet on chemical composition, in vitro gas production (IVGP) and rumen fermentation. The treatments applied were: A=cassava peel (20%)+ cassava meal (70%)+cassava leaves (5%)+moringa leaves (5%); B=cassava peel (20%)+ cassava meal (60%)+cassava leaves (10%)+moringa leaves (10%); C=cassava peel (20%)+ cassava meal (50%)+cassava leaves (15%)+moringa leaves (15%) cassava meal; D=cassava peel (20%)+ cassava meal (40%)+cassava leaves (20%)+moringa leaves (20%); E=cassava peel (20%)+ cassava meal (30%)+cassava leaves (25%)+moringa leaves (25%) with 3 replications arranged in a Randomized Block Design (RBD). The result showed that the increasing levels of cassava meal in the ration significantly increased organic matter (OM) and nitrogen-free extract (NFE) content (P<0.05), but reduced crude protein (CP), ether extract (EE), crude fiber (CF), neutral detergent fiber (NDF), and acid detergent fiber (ADF) contents. Similarly, a significant increased (P<0.01) were found in values of cumulative in vitro gas production, dry matter and organic matter digestibility, but decreased NH3 concentration (P<0.05) due to the increased of cassava meal. It is concluded that increasing levels of cassava meal in concentrate has led to higher OM content of the ration and being available for rumen fermentation.

Chemical composition and in vitro gas production of cassava peel silage using different preservatives

This study aimed to evaluate the effects of using different types and levels of preservatives on chemical composition and in vitro gas production (IVGP) of cassava peel silage. The following treatments were applied: T0: cassava peel without preservative; T1: T0 + molasses 2%; T2: T0 + molasses 4%; T3: T0 + rice bran 2%; T4: T0 + rice bran 4%; T5: T0 + cassava meal 2% and T6:T0 + cassava meal 4% with 3 replications arranged in a completely randomized design (CRD) for the chemical composition variable and randomized block design (RBD) for IVGP variable. The results showed that treatments significantly affected (P<0.01) DM, OM, and EE contents, significantly affected (P<0,05) IVGP but did not significantly affect CP, CF contents, potential, and rate of gas production (P>0.05). Treatment T5 seems to have higher values of IVGP and ME (159.1±7.19 ml/500 mgDM; 11.1±0.36MJ/kg DM, respectively) compared to the other treatments that might have related to a higher OM available for in vitro fermentation process. It can be concluded that the use of cassava meal at 2% level can be considered as the most ideal preservative to use to produce a high-quality cassava peel silage.

PSX-B-8 Effect of supplementing red osier dogwood extract on in vitro gas production, feed digestibility and fermentation characteristics of high-forage diet

Red osier dogwood (ROD) is native shrub plant in Canada and rich in bioactive compounds. The objective of this study was to evaluate the effects of ROD extract supplementation in a high-forage (HF) diet on gas production (GP), dry matter (DM) disappearance (DMD) and fermentation characteristics in batch cultures with varying media pH. The study was a completely randomized design with 4 levels of ROD extract (0, 1, 3 and 5% of substrate) × 2 media pH (5.8 and 6.5) factorial arrangement. The study was conducted in three replicate experiments. Substrate contained 60% barley silage and 40% barley concentrate (DM basis). Inoculum was obtained from 2 ruminally fistulated beef heifers offered the HF diet. Substrate (0.5 g DM) ground (1 mm) was incubated for 48 h in a culture bottle. Data were analyzed using Mixed procedure of SAS with fixed effects of treatments and random effect of experiment. There was no interaction between media pH and level of ROD on GP, DMD and fermentation characteristics. Increased media pH (5.8 vs. 6.5) increased (P &lt; 0.01) GP (averaged 164 vs. 275 ml/g substrate), DMD (50.6 vs. 60.6%), and total VFA production (63 vs. 71 mM). Increasing ROD extract levels did not affect GP but linearly (P = 0.05) decreased DMD from 62.1 to 58.9% at pH 6.5. Although total VFA production was not affected, increasing ROD extract linearly (P &lt; 0.01) increased the proportion of acetate (47.9 to 49.7%) and propionate (20.5 to 22.3%) at pH 5.8; whereas at pH 6.5, acetate to propionate ratio quadratically (P = 0.04) changed to be lower with 3% ROD extract (2.08) than other treatments (2.21). These results indicated that supplementation of HF diet with ROD extract may improve fibre digestion at low media pH, and improve fermentation efficiency at high media pH.