scholarly journals Top-Down and Bottom-Up Mass Spectrometric Characterization of Human Myoglobin-Centered Free Radicals Induced by Oxidative Damage

2007 ◽  
Vol 79 (16) ◽  
pp. 6236-6248 ◽  
Author(s):  
Leesa J. Deterding ◽  
Suchandra Bhattacharjee ◽  
Dario C. Ramirez ◽  
Ronald P. Mason ◽  
Kenneth B. Tomer
Keyword(s):  
Free Radicals ◽  
Oxidative Damage ◽  
Mass Spectrometric ◽  
Top Down ◽  
Bottom Up ◽  
Human Myoglobin

Combined Top-Down and Bottom-Up Mass Spectrometric Approach to Characterization of Biomarkers for Renal Disease

2005 ◽  
Vol 77 (22) ◽  
pp. 7163-7171 ◽  
Author(s):  
Michael J. Chalmers ◽  
Colin Logan Mackay ◽  
Christopher L. Hendrickson ◽  
Stefan Wittke ◽  
Michael Walden ◽  
...  
Keyword(s):  
Renal Disease ◽  
Mass Spectrometric ◽  
Top Down ◽  
Bottom Up ◽  
Mass Spectrometric Approach

Glycoproteomics: a powerful tool for characterizing the diverse glycoforms of bacterial pilins and flagellins

2010 ◽  
Vol 38 (5) ◽  
pp. 1307-1313 ◽  
Author(s):  
Paul G. Hitchen ◽  
Katie Twigger ◽  
Esmeralda Valiente ◽  
Rebecca H. Langdon ◽  
Brendan W. Wren ◽  
...  
Keyword(s):  
Structural Analysis ◽  
Current Knowledge ◽  
Genomic Data ◽  
High Sensitivity ◽  
Mass Spectrometric ◽  
Top Down ◽  
Bottom Up ◽  
Bacterial Proteins

With glycosylation now firmly established across both Archaeal and bacterial proteins, a wide array of glycan diversity has become evident from structural analysis and genomic data. These discoveries have been built in part on the development and application of mass spectrometric technologies to the bacterial glycoproteome. This review highlights recent findings using high sensitivity MS of the large variation of glycans that have been reported on flagellin and pilin proteins of bacteria, using both ‘top down’ and ‘bottom up’ approaches to the characterization of these glycoproteins. We summarize current knowledge of the sugar modifications that have been observed on flagellins and pilins, in terms of both the diverse repertoire of monosaccharides observed, and the assemblage of moieties that decorate many of these sugars.

scholarly journals An integrated strategy reveals complex glycosylation of erythropoietin using top-down and bottom-up mass spectrometry

2021 ◽  
Author(s):  
Yudong Guan ◽  
Min Zhang ◽  
Manasi Gaikwad ◽  
Hannah Voss ◽  
Ramin Fazel ◽  
...  
Keyword(s):  
First Generation ◽  
Mass Spectrometric ◽  
Protein Glycosylation ◽  
Qualitative And Quantitative Analysis ◽  
Top Down ◽  
Bottom Up ◽  
Qualitative And Quantitative ◽  
Integrated Strategy ◽  
Mass Shifts

ABSTRACTThe characterization of glycoproteins, like erythropoietin, is challenging due to the structural micro- and macro-heterogeneity of the protein glycosylation. This study presents an in-depth strategy for glycosylation analysis of a first-generation erythropoietin (epoetin beta), including a developed top-down mass spectrometric workflow for N-glycan analysis, bottom-up mass spectrometric methods for site-specific N-glycosylation and a LC-MS approach for O-glycan identification. Permethylated N-glycans, peptides and enriched glycopeptides of erythropoietin were analyzed by nanoLC-MS/MS and de-N-glycosylated erythropoietin was measured by LC-MS, enabling the qualitative and quantitative analysis of glycosylation and different glycan modifications (e.g., phosphorylation and O-acetylation). Extending the coverage of our newly developed Python script to phosphorylated N-glycans enabled the identification of 140 N-glycan compositions (237 N-glycan structures) from erythropoietin. The site-specificity of N-glycans was revealed at glycopeptide level by pGlyco software using different proteases. In total, 215 N-glycan compositions were identified from N-glycan and glycopeptide analysis. Moreover, LC-MS analysis of de-N-glycosylated erythropoietin species identified two different O-glycan compositions, based on the mass shifts between non-O-glycosylated and O-glycosylated species. This integrated strategy allows the in-depth glycosylation analysis of a therapeutic glycoprotein to understand its pharmacological properties and improving the manufacturing processes.

Characterization of the 70S Ribosome fromRhodopseudomonaspalustrisUsing an Integrated “Top-Down” and “Bottom-Up” Mass Spectrometric Approach

2004 ◽  
Vol 3 (5) ◽  
pp. 965-978 ◽  
Author(s):  
Michael Brad Strader ◽  
Nathan C. VerBerkmoes ◽  
David L. Tabb ◽  
Heather M. Connelly ◽  
John W. Barton ◽  
...  
Keyword(s):  
Mass Spectrometric ◽  
Top Down ◽  
Bottom Up ◽  
Mass Spectrometric Approach ◽  
70S Ribosome

Ambivalences of populism: The case of Catalan independentism

2018 ◽  
Vol 57 (4) ◽  
pp. 573-587 ◽  
Author(s):  
Daniel Gamper Sachse
Keyword(s):  
Social Mobilization ◽  
Top Down ◽  
Bottom Up ◽  
Democratic Experience ◽  
Procedural Safeguards

Most of the Spanish media have portrayed Catalan independentism as nationalism and populism. This article argues that this political and social mobilization does not fit into the usual definitions of populism as anti-elitist, anti-liberal and anti-pluralistic movement. Catalan independentism is here interpreted by stressing its democratic features, namely as a form of democratic populism: horizontally organized, and critical of procedural safeguards and counter-majoritarian powers. The popular organization of the referendum on 1 October 2017 and the vigorous democratic experience by almost half of the population of Catalonia allow for a characterization of this populism as a hybrid phenomenon that includes bottom-up and top-down dynamics, thereby contrasting with the usual leader-centred understanding of populism.

scholarly journals Fragmentation methods on the balance: unambiguous top–down mass spectrometric characterization of oxaliplatin–ubiquitin binding sites

2011 ◽  
Vol 402 (8) ◽  
pp. 2655-2662 ◽  
Author(s):  
Samuel M. Meier ◽  
Yury O. Tsybin ◽  
Paul J. Dyson ◽  
Bernhard K. Keppler ◽  
Christian G. Hartinger
Keyword(s):  
Binding Sites ◽  
Mass Spectrometric ◽  
Top Down ◽  
Ubiquitin Binding

Characterization of Human Sperm Protamine Proteoforms through a Combination of Top-Down and Bottom-Up Mass Spectrometry Approaches

2019 ◽  
Vol 19 (1) ◽  
pp. 221-237 ◽  
Author(s):  
Ada Soler-Ventura ◽  
Marina Gay ◽  
Meritxell Jodar ◽  
Mar Vilanova ◽  
Judit Castillo ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Human Sperm ◽  
Top Down ◽  
Bottom Up

scholarly journals A functional context for heterogeneity of the circadian clock in cells

PLoS Biology ◽  
2020 ◽  
Vol 18 (10) ◽  
pp. e3000927
Author(s):  
Martha Merrow ◽  
Mary Harrington
Keyword(s):  
Circadian Clock ◽  
Building Blocks ◽  
Circadian Period ◽  
Top Down ◽  
Bottom Up ◽  
Stochastic Generation ◽  
Circadian Phase ◽  
Functional Context ◽  
Definition Of

Characterization of circadian systems at the organism level—a top-down approach—has led to definition of unifying properties, a hallmark of the science of chronobiology. The next challenge is to use a bottom-up approach to show how the molecular workings of the cellular circadian clock work as building blocks of those properties. We review new studies, including a recently published PLOS Biology paper by Nikhil and colleagues, that show how programmed but also stochastic generation of variation in cellular circadian period explain important adaptive features of entrained circadian phase.

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