scholarly journals High-Throughput Method Development for Sensitive, Accurate, and Reproducible Quantification of Therapeutic Monoclonal Antibodies in Tissues Using Orthogonal Array Optimization and Nano Liquid Chromatography/Selected Reaction Monitoring Mass Spectrometry

2012 ◽  
Vol 84 (10) ◽  
pp. 4373-4382 ◽  
Author(s):  
Xiaotao Duan ◽  
Lubna Abuqayyas ◽  
Lipeng Dai ◽  
Joseph P. Balthasar ◽  
Jun Qu
Keyword(s):  
Mass Spectrometry ◽  
Monoclonal Antibodies ◽  
Liquid Chromatography ◽  
Method Development ◽  
Selected Reaction Monitoring ◽  
Reaction Monitoring ◽  
High Throughput Method ◽  
Array Optimization ◽  
Nano Liquid Chromatography ◽  
Therapeutic Monoclonal Antibodies

scholarly journals LC-MS/MS Method Development for the Discovery and Identification of Amphidinols Produced by Amphidinium

Marine Drugs ◽  
2020 ◽  
Vol 18 (10) ◽  
pp. 497
Author(s):  
Marvin Wellkamp ◽  
Francisco García-Camacho ◽  
Lorena M. Durán-Riveroll ◽  
Jan Tebben ◽  
Urban Tillmann ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Method Development ◽  
Neutral Loss ◽  
Tandem Mass ◽  
Reaction Monitoring ◽  
Fish Kills ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
Fragmentation Patterns

Amphidinols are polyketides produced by dinoflagellates suspected of causing fish kills. Here, we demonstrate a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the identification and quantification of amphidinols (AM). Novel AM were detected by neutral loss (NL) scan and then quantified together with known AM by selection reaction monitoring (SRM). With the new method, AM were detected in four of eight analyzed strains with a maximum of 3680 fg toxin content per cell. In total, sixteen novel AM were detected by NL scan and characterized via their fragmentation patterns. Of these, two substances are glycosylated forms. This is the first detection of glycosylated AM.

Assessment of semi-permanent hair dyes in wash water from beauty salons by liquid chromatography-tandem mass spectrometry-selected reaction monitoring (LC-MS/MS-SRM)

2020 ◽  
Vol 12 (44) ◽  
pp. 5415-5423
Author(s):  
Jefferson Honorio Franco ◽  
Bianca F. da Silva ◽  
Maria V. B. Zanoni
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Wash Water ◽  
Selected Reaction Monitoring ◽  
Tandem Mass ◽  
Reaction Monitoring ◽  
Hair Dyes ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

Herein, we present an approach for the quantification of semi-permanent hair dyes in wash water samples released during washing of dyed hair employing a liquid chromatography-tandem mass spectrometry-selected reaction monitoring (LC-MS/MS-SRM).

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