Nomogram for adjusting mobile phase composition in reverse phase high pressure liquid chromatography

1980 ◽  
Vol 52 (8) ◽  
pp. 1370-1371 ◽  
Author(s):  
James L. Meek
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Phase Composition ◽  
High Pressure Liquid Chromatography ◽  
Mobile Phase ◽  
Mobile Phase Composition ◽  
Reverse Phase

A Simple Procedure for the Determination of Carbamazepine in Plasma by High Pressure-Liquid Chromatography

1979 ◽  
Vol 16 (1-6) ◽  
pp. 213-216 ◽  
Author(s):  
C. K. Johnston ◽  
G. H. Lester
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
Mobile Phase ◽  
Simple Procedure ◽  
Internal Standard ◽  
Reverse Phase ◽  
Coefficients Of Variation ◽  
Initial Results

We describe a simple, rapid procedure for the estimation of carbamazepine in plasma. Protein is precipitated, and extraction is achieved by the addition of acetonitrile containing the internal standard N-acetyltryptophan ethyl ester. Separation is by reverse-phase high-pressure liquid chromatography with an acetonitrile: water mobile phase, and detection is by UV absorption at 280 nm. Total retention time is less than 7 minutes. Initial results gave within-batch and between-batch coefficients of variation of less than 2 %, and mean recovery of 97 %. The method is free from interference by other common anticonvulsant drugs.

Simultaneous Determination of Vitamins A and D in Dosage Forms by High Pressure Liquid Chromatography

1982 ◽  
Vol 65 (3) ◽  
pp. 619-623
Author(s):  
Maria Ines ◽  
R M Santoro ◽  
João F Magalhães ◽  
Erika R M Hackmann
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Vitamin A ◽  
Petroleum Ether ◽  
High Pressure Liquid Chromatography ◽  
Mobile Phase ◽  
Dosage Forms ◽  
Reverse Phase ◽  
Solvent Residues

Abstract Vitamins A and D were determined simultaneously in oily solutions, ointments, and elixirs, but only vitamin A could be determined in capsules. Samples were saponified with KOH in isopropanol-water, using hydroquinone as antioxidant, and extracted with ether-petroleum ether (1 + 1). After evaporation of solvent, residues were dissolved in isopropanol. Vitamins in these solutions were determined by reverse phase high pressure liquid chromatography, using methanol-water as mobile phase and detection at 254 nm. The reproducibility, using external standards, was 1.6-2.5% and 1.2-3.8% for vitamins A and D, respectively.

MAGIC-LC/FT-IR Spectrometry: Preliminary Studies

1988 ◽  
Vol 42 (8) ◽  
pp. 1365-1368 ◽  
Author(s):  
R. M. Robertson ◽  
J. A. De Haseth ◽  
J. D. Kirk ◽  
R. F. Browner
Keyword(s):  
Phase Composition ◽  
Mobile Phase ◽  
Mobile Phase Composition ◽  
Reverse Phase ◽  
Ir Spectrometry ◽  
Aerosol Generation ◽  
Mobile Phases ◽  
Ft Ir ◽  
Effluent Stream ◽  
Phase Water

A new solvent elimination interface based on the Monodisperse Aerosol Generation Interface for Combining Liquid Chromatography with Fourier transform infrared (MAGIC-LC/FT-IR) spectrometry is described. The solvent elimination efficiency of MAGIC-LC/FT-IR was studied by varying the mobile-phase composition from 100% methanol to 100% water. As the mobile-phase composition was varied, erythrosin B was injected into the interface and deposited on a KBr window after the solvent removal. Spectra were obtained which compared favorably with reference spectra, even as the mobile-phase water content was increased. A reverse-phase separation was completed to demonstrate that readily identifiable spectra can be obtained from mobile phases containing high percentages of water, without heating of the effluent stream.

Screening Method for Vitamins A and D in Fortified Skim Milk, Chocolate Milk, and Vitamin D Liquid Concentrates

1979 ◽  
Vol 62 (6) ◽  
pp. 1358-1360
Author(s):  
Susan K Henderson ◽  
Lucia A Mclean
Keyword(s):  
Vitamin D ◽  
High Pressure ◽  
Liquid Chromatography ◽  
Vitamin A ◽  
Mobile Phase ◽  
Screening Method ◽  
Skim Milk ◽  
Chocolate Milk ◽  
Reverse Phase ◽  
Milk Chocolate

Abstract Vitamin A was determined in fortified chocolate milk and skim milk; vitamin D was determined in fortified chocolate milk, skim milk, and vitamin D concentrates, using reverse phase high pressure liquid chromatography (HPLC). The sample is saponified, extracted with hexane, and chromatographed in an HPLC system on a 10 μm Vydac TP reverse phase C18 column, using acetonitrile-methanol (9+1) as the mobile phase. For 6 replicates, the recoveries of vitamins A and D, using this procedure, were 99 and 98%, respectively.

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