Sensitive Surface-Enhanced Raman Spectroscopy (SERS) Detection of Organochlorine Pesticides by Alkyl Dithiol-Functionalized Metal Nanoparticles-Induced Plasmonic Hot Spots

2014 ◽  
Vol 87 (1) ◽  
pp. 663-669 ◽  
Author(s):  
Jana Kubackova ◽  
Gabriela Fabriciova ◽  
Pavol Miskovsky ◽  
Daniel Jancura ◽  
Santiago Sanchez-Cortes
Keyword(s):  
Raman Spectroscopy ◽  
Metal Nanoparticles ◽  
Organochlorine Pesticides ◽  
Hot Spots ◽  
Surface Enhanced Raman Spectroscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Sers Detection

Multivariate Analysis Aided Surface-Enhanced Raman Spectroscopy (MVA-SERS) Multiplex Quantitative Detection of Trace Fentanyl in Illicit Drug Mixtures Using a Handheld Raman Spectrometer

2021 ◽  
pp. 000370282110329
Author(s):  
Ling Wang ◽  
Mario O. Vendrell-Dones ◽  
Chiara Deriu ◽  
Sevde Doğruer ◽  
Peter de B. Harrington ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Principal Component ◽  
Surface Enhanced Raman Spectroscopy ◽  
Quantitative Detection ◽  
Least Squares Regression ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Sers Detection ◽  
Current Screening

Recently there has been upsurge in reports that illicit seizures of cocaine and heroin have been adulterated with fentanyl. Surface-enhanced Raman spectroscopy (SERS) provides a useful alternative to current screening procedures that permits detection of trace levels of fentanyl in mixtures. Samples are solubilized and allowed to interact with aggregated colloidal nanostars to produce a rapid and sensitive assay. In this study, we present the quantitative determination of fentanyl in heroin and cocaine using SERS, using a point-and-shoot handheld Raman system. Our protocol is optimized to detect pure fentanyl down to 0.20 ± 0.06 ng/mL and can also distinguish pure cocaine and heroin at ng/mL levels. Multiplex analysis of mixtures is enabled by combining SERS detection with principal component analysis and super partial least squares regression discriminate analysis (SPLS-DA), which allow for the determination of fentanyl as low as 0.05% in simulated seized heroin and 0.10% in simulated seized cocaine samples.

scholarly journals Porous carbon nanowire array for surface-enhanced Raman spectroscopy

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Nan Chen ◽  
Ting-Hui Xiao ◽  
Zhenyi Luo ◽  
Yasutaka Kitahama ◽  
Kotaro Hiramatsu ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Porous Carbon ◽  
Hot Spots ◽  
Strong Dependence ◽  
Nanowire Array ◽  
Surface Enhanced Raman Spectroscopy ◽  
Localized Surface Plasmon ◽  
Sers Substrate ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

Abstract Surface-enhanced Raman spectroscopy (SERS) is a powerful tool for vibrational spectroscopy as it provides several orders of magnitude higher sensitivity than inherently weak spontaneous Raman scattering by exciting localized surface plasmon resonance (LSPR) on metal substrates. However, SERS can be unreliable for biomedical use since it sacrifices reproducibility, uniformity, biocompatibility, and durability due to its strong dependence on “hot spots”, large photothermal heat generation, and easy oxidization. Here, we demonstrate the design, fabrication, and use of a metal-free (i.e., LSPR-free), topologically tailored nanostructure composed of porous carbon nanowires in an array as a SERS substrate to overcome all these problems. Specifically, it offers not only high signal enhancement (~106) due to its strong broadband charge-transfer resonance, but also extraordinarily high reproducibility due to the absence of hot spots, high durability due to no oxidization, and high compatibility to biomolecules due to its fluorescence quenching capability.

Probing the Location of Hot Spots by Surface-Enhanced Raman Spectroscopy: Toward Uniform Substrates

ACS Nano ◽  
2013 ◽  
Vol 8 (1) ◽  
pp. 528-536 ◽  
Author(s):  
Xiang Wang ◽  
Maohua Li ◽  
Lingyan Meng ◽  
Kaiqiang Lin ◽  
Jiamin Feng ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Hot Spots ◽  
Surface Enhanced Raman Spectroscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

scholarly journals Optimization of Surface-Enhanced Raman Spectroscopy Detection Conditions for Interaction between Gonyautoxin and Its Aptamer

Toxins ◽  
2022 ◽  
Vol 14 (1) ◽  
pp. 49
Author(s):  
Yan Liu ◽  
Lijuan He ◽  
Yunli Zhao ◽  
Yongbing Cao ◽  
Zhiguo Yu ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Surface Enhanced Raman Spectroscopy ◽  
Colloid Solution ◽  
Silver Colloid ◽  
Enrichment Technique ◽  
Surface Enhanced ◽  
G Quadruplex ◽  
Surface Enhanced Raman ◽  
Sers Detection ◽  
Exponential Enrichment

This study aimed to optimize the detection conditions for surface-enhanced Raman spectroscopy (SERS) of single-stranded DNA (ssDNA) in four different buffers and explore the interaction between gonyautoxin (GTX1/4) and its aptamer, GO18. The influence of the silver colloid solution and MgSO4 concentration (0.01 M) added under four different buffered conditions on DNA SERS detection was studied to determine the optimum detection conditions. We explored the interaction between GTX1/4 and GO18 under the same conditions as those in the systematic evolution of ligands by exponential enrichment technique, using Tris-HCl as the buffer. The characteristic peaks of GO18 and its G-quadruplex were detected in four different buffer solutions. The change in peak intensity at 1656 cm−1 confirmed that the binding site between GTX1/4 and GO18 was in the G-quadruplex plane. The relative intensity of the peak at 1656 cm−1 was selected for the GTX1/4–GO18 complex (I1656/I1099) to plot the ratio of GTX1/4 in the Tris-HCl buffer condition (including 30 μL of silver colloid solution and 2 μL of MgSO4), and a linear relationship was obtained as follows: Y = 0.1867X + 1.2205 (R2 = 0.9239). This study provides a basis for subsequent application of SERS in the detection of ssDNA, as well as the binding of small toxins and aptamers.

scholarly journals Ag@Au Core–Shell Porous Nanocages with Outstanding SERS Activity for Highly Sensitive SERS Immunoassay

Sensors ◽  
2019 ◽  
Vol 19 (7) ◽  
pp. 1554 ◽  
Author(s):  
Yaqi Huang ◽  
Dajie Lin ◽  
Mengting Li ◽  
Dewu Yin ◽  
Shun Wang ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Raman Spectroscopy ◽  
Hot Spots ◽  
Signal Amplification ◽  
Surface Enhanced Raman Spectroscopy ◽  
Core Shell ◽  
Chloroauric Acid ◽  
Highly Sensitive ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

A highly sensitive immunoassay of biomarkers has been achieved using 4-mercaptobenzoic acid-labeled Ag@Au core–shell porous nanocage tags and α-fetoprotein immuno-sensing chips. The Ag@Au porous nanocages were uniquely synthesized by using an Ag core as a self-sacrificial template and reducing agent, where the slow reaction process led to the formation of a porous Au layer. The size of the remaining Ag core and surface roughness of the Au shell were controlled by adjusting the chloroauric acid concentration. The porous cage exhibited excellent surface-enhanced Raman spectroscopy (SERS) activity, presumably due to a synergetic interaction between newly generated hot spots in the rough Au shell and the retained SERS activity of the Ag core. Using α-fetoprotein as a model analyte for immunoassay, the SERS signal had a wide linear range of 0.20 ng mL−1 to 500.0 ng mL−1 with a detection limit of 0.12 ng mL−1. Without the need of further signal amplification, the as-prepared Ag@Au bimetallic nanocages can be directly used for highly sensitive SERS assays of other biomarkers in biomedical research, diagnostics, etc.

Sign in / Sign up

Export Citation Format

Share Document