Emulsion PCR Significantly Improves Nonequilibrium Capillary Electrophoresis of Equilibrium Mixtures-Based Aptamer Selection: Allowing for Efficient and Rapid Selection of Aptamer to Unmodified ABH2 Protein

A single-round DNA aptamer selection for mammalian cells was successfully achieved for the first time using a capillary electrophoresis (CE)-based methodology.

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Detection and classification of paraproteins by capillary immunofixation/subtraction

Clinical Chemistry ◽

10.1093/clinchem/44.4.760 ◽

1998 ◽

Vol 44 (4) ◽

pp. 760-764 ◽

Cited By ~ 37

Author(s):

Xavier Bossuyt ◽

Ann Bogaerts ◽

Gilberte Schiettekatte ◽

Norbert Blanckaert

Keyword(s):

Capillary Electrophoresis ◽

Cellulose Acetate ◽

Cellulose Acetate Electrophoresis ◽

Detection And Identification ◽

Agarose Electrophoresis ◽

Oligoclonal Banding ◽

Capillary Zone ◽

Electrophoresis System ◽

Selection Of

Abstract A selection of 58 specimens with a monoclonal component identified by immunoelectrophoresis and/or immunofixation was analyzed with the immunosubtraction procedure on the Paragon 2000 capillary electrophoresis system. The capillary system detected 93% of the paraproteins and, using immunosubtraction, correctly identified 91% of the paraproteins. Paraproteins that were detected by immunofixation and/or immunoelectrophoresis but not by capillary electrophoresis were also missed by agarose electrophoresis and cellulose acetate electrophoresis. Cellulose acetate electrophoresis was the least sensitive method for detection of paraproteins. Only 74% of the monoclonal components were detected by this technique, whereas 86% were revealed by agarose electrophoresis. In addition to monoclonal paraproteins, we also studied biclonal paraproteins and oligoclonal banding. Capillary electrophoresis and immunosubtraction correctly detected and identified three specimens containing biclonal paraproteins. In one specimen, capillary zone electrophoresis detected only one band, whereas agarose gel electrophoresis detected two bands. The sensitivity for detection and identification of oligoclonal banding by capillary electrophoresis was inferior to immunofixation.

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Selection of Smart Aptamers by Methods of Kinetic Capillary Electrophoresis

Analytical Chemistry ◽

10.1021/ac060144h ◽

2006 ◽

Vol 78 (9) ◽

pp. 3171-3178 ◽

Cited By ~ 89

Author(s):

Andrei P. Drabovich ◽

Maxim Berezovski ◽

Victor Okhonin ◽

Sergey N. Krylov

Keyword(s):

Capillary Electrophoresis ◽

Kinetic Capillary Electrophoresis ◽

Selection Of

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High-efficiency selection of aptamers for bovine lactoferrin by capillary electrophoresis and its aptasensor application in milk powder

Talanta ◽

10.1016/j.talanta.2019.06.088 ◽

2019 ◽

Vol 205 ◽

pp. 120088 ◽

Cited By ~ 6

Author(s):

Chao Zhu ◽

Linsen Li ◽

Ge Yang ◽

Muhammad Irfan ◽

Zijian Wang ◽

...

Keyword(s):

Capillary Electrophoresis ◽

High Efficiency ◽

Milk Powder ◽

Bovine Lactoferrin ◽

Selection Of

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In Silico Selection of Gp120 ssDNA Aptamer to HIV-1

SLAS DISCOVERY Advancing Life Sciences ◽

10.1177/2472555220923331 ◽

2020 ◽

Vol 25 (9) ◽

pp. 1087-1093

Author(s):

Hamideh Sepehri Zarandi ◽

Mandana Behbahani ◽

Hassan Mohabatkar

Keyword(s):

In Silico ◽

Dna Aptamer ◽

Surface Glycoprotein ◽

Aptamer Selection ◽

Glycoprotein Gp120 ◽

Systematic Evolution ◽

Exponential Enrichment ◽

Hiv 1 ◽

Aptamer Sequence ◽

Selection Of

Nucleic acid aptamers that specifically bind to other molecules are mostly obtained through the systematic evolution of ligands by exponential enrichment (SELEX). Because SELEX is a time-consuming procedure, the in silico design of specific aptamers has recently become a progressive approach. HIV-1 surface glycoprotein gp120, which is involved in the early stages of HIV-1 infection, is an attractive target for RNA and DNA aptamer selection. In this study, four single-stranded DNA aptamers, referred to as HD2, HD3, HD4, and HD5, that had the ability of HIV-1 inhibition were designed in silico. In a proposed non-SELEX approach, some parts of the B40 aptamer sequence, which interacted with gp120, were isolated and considered as a separate aptamer sequence. Then, to obtain the best docking scores of the HDOCK server and Hex software, some modifications, insertions, and deletions were applied to each selected sequence. Finally, the cytotoxicity and HIV inhibition of the selected aptamers were evaluated experimentally. Results demonstrated that the selected aptamers could inhibit HIV-1 infection by up to 80%, without any cytotoxicity. Therefore, this new non-SELEX approach could be considered a simple, fast, and efficient method for aptamer selection.

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Determination of Histamine in Tomatoes by Capillary Electrophoresis

Journal of AOAC International ◽

10.1093/jaoac/83.1.89 ◽

2000 ◽

Vol 83 (1) ◽

pp. 89-94 ◽

Cited By ~ 1

Author(s):

Elek Bolygo ◽

Paul A Cooper ◽

K Michael Jessop ◽

Frank Moffatt

Keyword(s):

Capillary Electrophoresis ◽

Signal To Noise Ratio ◽

High Sensitivity ◽

Flow Cell ◽

Voltage Gradient ◽

Signal To Noise ◽

Tomato Fruits ◽

Endogenous Level ◽

Selection Of

Abstract An improved capillary electrophoresis assay for histamine in crude extracts was developed and used to determine histamine levels in a selection of tomato fruits and pastes. Performance in terms of reproducibility and sensitivity was optimized by use of a high sensitivity detector flow cell, sodium hydroxide rinses, and a voltage gradient. The method was linear down to 0.2 μg/mL (signal-to-noise ratio = 4:1), which was below the endogenous level in all samples.

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